LOCUS       sequence-180313-       10648 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses SypHer in plant cells-biosensor for pH.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10648)
  AUTHORS   Exposito-Rodriguez M, Laissue PP, Yvon-Durocher G, Smirnoff N, 
            Mullineaux PM
  TITLE     Photosynthesis-dependent H2O2 transfer from chloroplasts to nuclei 
            provides a high-light signalling mechanism.
  JOURNAL   Nat Commun. 2017 Jun 29;8(1):49. doi: 10.1038/s41467-017-00074-w.
  PUBMED    28663550
REFERENCE   2  (bases 1 to 10648)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2017-06-29"; volume: "8(1):49. doi"; pages: " 
            10.1038/s41467-017-00074-w"
COMMENT     Sequence Label: pUB-cSypHer
FEATURES             Location/Qualifiers
     source          1..10648
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(56..72)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(56..72)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(69..91)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    80..96
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(104..110,111..128,129..134))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    149..170
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    335..359
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             1690..2319
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             2748..3821
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      3887..4081
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(4317..4339)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    4425..4565
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     4480..4499
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4580..4597)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4751..5339)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4831..4850)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(5585..6376)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_feature    6897..6921
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(7014..7266)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(7286..7837)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(7879..8058)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     protein_bind    8480..8504
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      9963..9972
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(9989..10013)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ctttgacgcc gtttattact gactcgtcgg gtaccgagct cgaattcgta atcatgtcat
       61 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
      121 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
      181 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
      241 aacgcgcggg gagaggcggt ttgcgtattg gctagagcag cttgagcttg gatcagattg
      301 tcgtttcccg ccttcagttt aaactatcag tgtttgacag gatatattgg cgggtaaacc
      361 taagagaaaa gagcgtttat tagaataatc ggatatttaa aagggcgtga aaaggtttat
      421 ccgttcgtcc atttgtatgt gcatgccaac cacagggttc ccctcgggat caaagtactt
      481 taaagtactt taaagtactt taaagtactt tgatccaacc cctccgctgc tatagtgcag
      541 tcggcttctg acgttcagtg cagccgtctt ctgaaaacga catgtcgcac aagtcctaag
      601 ttacgcgaca ggctgccgcc ctgccctttt cctggcgttt tcttgtcgcg tgttttagtc
      661 gcataaagta gaatacttgc gactagaacc ggagacatta cgccatgaac aagagcgccg
      721 ccgctggcct gctgggctat gcccgcgtca gcaccgacga ccaggacttg accaaccaac
      781 gggccgaact gcacgcggcc ggctgcacca agctgttttc cgagaagatc accggcacca
      841 ggcgcgaccg cccggagctg gccaggatgc ttgaccacct acgccctggc gacgttgtga
      901 cagtgaccag gctagaccgc ctggcccgca gcacccgcga cctactggac attgccgagc
      961 gcatccagga ggccggcgcg ggcctgcgta gcctggcaga gccgtgggcc gacaccacca
     1021 cgccggccgg ccgcatggtg ttgaccgtgt tcgccggcat tgccgagttc gagcgttccc
     1081 taatcatcga ccgcacccgg agcgggcgcg aggccgccaa ggcccgaggc gtgaagtttg
     1141 gcccccgccc taccctcacc ccggcacaga tcgcgcacgc ccgcgagctg atcgaccagg
     1201 aaggccgcac cgtgaaagag gcggctgcac tgcttggcgt gcatcgctcg accctgtacc
     1261 gcgcacttga gcgcagcgag gaagtgacgc ccaccgaggc caggcggcgc ggtgccttcc
     1321 gtgaggacgc attgaccgag gccgacgccc tggcggccgc cgagaatgaa cgccaagagg
     1381 aacaagcatg aaaccgcacc aggacggcca ggacgaaccg tttttcatta ccgaagagat
     1441 cgaggcggag atgatcgcgg ccgggtacgt gttcgagccg cccgcgcacg tctcaaccgt
     1501 gcggctgcat gaaatcctgg ccggtttgtc tgatgccaag ctggcggcct ggccggccag
     1561 cttggccgct gaagaaaccg agcgccgccg tctaaaaagg tgatgtgtat ttgagtaaaa
     1621 cagcttgcgt catgcggtcg ctgcgtatat gatgcgatga gtaaataaac aaatacgcaa
     1681 ggggaacgca tgaaggttat cgctgtactt aaccagaaag gcgggtcagg caagacgacc
     1741 atcgcaaccc atctagcccg cgccctgcaa ctcgccgggg ccgatgttct gttagtcgat
     1801 tccgatcccc agggcagtgc ccgcgattgg gcggccgtgc gggaagatca accgctaacc
     1861 gttgtcggca tcgaccgccc gacgattgac cgcgacgtga aggccatcgg ccggcgcgac
     1921 ttcgtagtga tcgacggagc gccccaggcg gcggacttgg ctgtgtccgc gatcaaggca
     1981 gccgacttcg tgctgattcc ggtgcagcca agcccttacg acatatgggc caccgccgac
     2041 ctggtggagc tggttaagca gcgcattgag gtcacggatg gaaggctaca agcggccttt
     2101 gtcgtgtcgc gggcgatcaa aggcacgcgc atcggcggtg aggttgccga ggcgctggcc
     2161 gggtacgagc tgcccattct tgagtcccgt atcacgcagc gcgtgagcta cccaggcact
     2221 gccgccgccg gcacaaccgt tcttgaatca gaacccgagg gcgacgctgc ccgcgaggtc
     2281 caggcgctgg ccgctgaaat taaatcaaaa ctcatttgag ttaatgaggt aaagagaaaa
     2341 tgagcaaaag cacaaacacg ctaagtgccg gccgtccgag cgcacgcagc agcaaggctg
     2401 caacgttggc cagcctggca gacacgccag ccatgaagcg ggtcaacttt cagttgccgg
     2461 cggaggatca caccaagctg aagatgtacg cggtacgcca aggcaagacc attaccgagc
     2521 tgctatctga atacatcgcg cagctaccag agtaaatgag caaatgaata aatgagtaga
     2581 tgaattttag cggctaaagg aggcggcatg gaaaatcaag aacaaccagg caccgacgcc
     2641 gtggaatgcc ccatgtgtgg aggaacgggc ggttggccag gcgtaagcgg ctgggttgtc
     2701 tgccggccct gcaatggcac tggaaccccc aagcccgagg aatcggcgtg agcggtcgca
     2761 aaccatccgg cccggtacaa atcggcgcgg cgctgggtga tgacctggtg gagaagttga
     2821 aggccgcgca ggccgcccag cggcaacgca tcgaggcaga agcacgcccc ggtgaatcgt
     2881 ggcaagcggc cgctgatcga atccgcaaag aatcccggca accgccggca gccggtgcgc
     2941 cgtcgattag gaagccgccc aagggcgacg agcaaccaga ttttttcgtt ccgatgctct
     3001 atgacgtggg cacccgcgat agtcgcagca tcatggacgt ggccgttttc cgtctgtcga
     3061 agcgtgaccg acgagctggc gaggtgatcc gctacgagct tccagacggg cacgtagagg
     3121 tttccgcagg gccggccggc atggccagtg tgtgggatta cgacctggta ctgatggcgg
     3181 tttcccatct aaccgaatcc atgaaccgat accgggaagg gaagggagac aagcccggcc
     3241 gcgtgttccg tccacacgtt gcggacgtac tcaagttctg ccggcgagcc gatggcggaa
     3301 agcagaaaga cgacctggta gaaacctgca ttcggttaaa caccacgcac gttgccatgc
     3361 agcgtacgaa gaaggccaag aacggccgcc tggtgacggt atccgagggt gaagccttga
     3421 ttagccgcta caagatcgta aagagcgaaa ccgggcggcc ggagtacatc gagatcgagc
     3481 tagctgattg gatgtaccgc gagatcacag aaggcaagaa cccggacgtg ctgacggttc
     3541 accccgatta ctttttgatc gatcccggca tcggccgttt tctctaccgc ctggcacgcc
     3601 gcgccgcagg caaggcagaa gccagatggt tgttcaagac gatctacgaa cgcagtggca
     3661 gcgccggaga gttcaagaag ttctgtttca ccgtgcgcaa gctgatcggg tcaaatgacc
     3721 tgccggagta cgatttgaag gaggaggcgg ggcaggctgg cccgatccta gtcatgcgct
     3781 accgcaacct gatcgagggc gaagcatccg ccggttccta atgtacggag cagatgctag
     3841 ggcaaattgc cctagcaggg gaaaaaggtc gaaaaggtct ctttcctgtg gatagcacgt
     3901 acattgggaa cccaaagccg tacattggga accggaaccc gtacattggg aacccaaagc
     3961 cgtacattgg gaaccggtca cacatgtaag tgactgatat aaaagagaaa aaaggcgatt
     4021 tttccgccta aaactcttta aaacttatta aaactcttaa aacccgcctg gcctgtgcat
     4081 aactgtctgg ccagcgcaca gccgaagagc tgcaaaaagc gcctaccctt cggtcgctgc
     4141 gctccctacg ccccgccgct tcgcgtcggc ctatcgcggc cgctggccgc tcaaaaatgg
     4201 ctggcctacg gccaggcaat ctaccagggc gcggacaagc cgcgccgtcg ccactcgacc
     4261 gccggcgccc acatcaaggc accctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc
     4321 tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga
     4381 caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag
     4441 tcacgtagcg atagcggagt gtatactggc ttaactatgc ggcatcagag cagattgtac
     4501 tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca
     4561 tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     4621 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     4681 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     4741 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     4801 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     4861 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     4921 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     4981 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     5041 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     5101 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     5161 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga
     5221 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     5281 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     5341 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     5401 ggattttggt catgcatgat atatctccca atttgtgtag ggcttattat gcacgcttaa
     5461 aaataataaa agcagacttg acctgatagt ttggctgtga gcaattatgt gcttagtgca
     5521 tctaacgctt gagttaagcc gcgccgcgaa gcggcgtcgg cttgaacgaa tttctagcta
     5581 gacattattt gccgactacc ttggtgatct cgcctttcac gtagtggaca aattcttcca
     5641 actgatctgc gcgcgaggcc aagcgatctt cttcttgtcc aagataagcc tgtctagctt
     5701 caagtatgac gggctgatac tgggccggca ggcgctccat tgcccagtcg gcagcgacat
     5761 ccttcggcgc gattttgccg gttactgcgc tgtaccaaat gcgggacaac gtaagcacta
     5821 catttcgctc atcgccagcc cagtcgggcg gcgagttcca tagcgttaag gtttcattta
     5881 gcgcctcaaa tagatcctgt tcaggaaccg gatcaaagag ttcctccgcc gctggaccta
     5941 ccaaggcaac gctatgttct cttgcttttg tcagcaagat agccagatca atgtcgatcg
     6001 tggctggctc gaagatacct gcaagaatgt cattgcgctg ccattctcca aattgcagtt
     6061 cgcgcttagc tggataacgc cacggaatga tgtcgtcgtg cacaacaatg gtgacttcta
     6121 cagcgcggag aatctcgctc tctccagggg aagccgaagt ttccaaaagg tcgttgatca
     6181 aagctcgccg cgttgtttca tcaagcctta cggtcaccgt aaccagcaaa tcaatatcac
     6241 tgtgtggctt caggccgcca tccactgcgg agccgtacaa atgtacggcc agcaacgtcg
     6301 gttcgagatg gcgctcgatg acgccaacta cctctgatag ttgagtcgat acttcggcga
     6361 tcaccgcttc ccccatgatg tttaactttg ttttagggcg actgccctgc tgcgtaacat
     6421 cgttgctgct ccataacatc aaacatcgac ccacggcgta acgcgcttgc tgcttggatg
     6481 cccgaggcat agactgtacc ccaaaaaaac agtcataaca agccatgaaa accgccactg
     6541 cgccgttacc accgctgcgt tcggtcaagg ttctggacca gttgcgtgag cgcatacgct
     6601 acttgcatta cagcttacga accgaacagg cttatgtcca ctgggttcgt gcccgaattg
     6661 atcacaggca gcaacgctct gtcatcgtta caatcaacat gctaccctcc gcgagatcat
     6721 ccgtgtttca aacccggcag cttagttgcc gttcttccga atagcatcgg taacatgagc
     6781 aaagtctgcc gccttacaac ggctctcccg ctgacgccgt cccggactga tgggctgcct
     6841 gtatcgagtg gtgattttgt gccgagctgc cggtcgggga gctgttggct ggctggtggc
     6901 aggatatatt gtggtgtaaa caaattgacg cttagacaac ttaataacac attgcggacg
     6961 tttttaatgt actgaattaa cgccgaattg aattatcagc ttgcatgccg gtcgatctag
     7021 taacatagat gacaccgcgc gcgataattt atcctagttt gcgcgctata ttttgttttc
     7081 tatcgcgtat taaatgtata attgcgggac tctaatcata aaaacccatc tcataaataa
     7141 cgtcatgcat tacatgttaa ttattacatg cttaacgtaa ttcaacagaa attatatgat
     7201 aatcatcgca agaccggcaa caggattcaa tcttaagaaa ctttattgcc aaatgtttga
     7261 acgatctgct tgactctagg ggtcatcaga tttcggtgac gggcaggacc ggacggggcg
     7321 gcaccggcag gctgaagtcc agctgccaga aacccacgtc atgccagttc ccgtgcttga
     7381 agccggccgc ccgcagcatg ccgcgggggg catatccgag cgcctcgtgc atgcgcacgc
     7441 tcgggtcgtt gggcagcccg atgacagcga ccacgctctt gaagccctgt gcctccaggg
     7501 acttcagcag gtgggtgtag agcgtggagc ccagtcccgt ccgctggtgg cggggggaga
     7561 cgtacacggt cgactcggcc gtccagtcgt aggcgttgcg tgccttccag ggacccgcgt
     7621 aggcgatgcc ggcgacctcg ccgtccacct cggcgacgag ccagggatag cgctcccgca
     7681 gacggacgag gtcgtccgtc cactcctgcg gttcctgcgg ctcggtacgg aagttgaccg
     7741 tgcttgtctc gatgtagtgg ttgacgatgg tgcagaccgc cggcatgtcc gcctcggtgg
     7801 cacggcggat gtcggccggg cgtcgttctg ggctcatggt agatcccctc gatcgagttg
     7861 agagtgaata tgagactcta attggatacc gaggggaatt tatggaacgt cagtggagca
     7921 tttttgacaa gaaatatttg ctagctgata gtgaccttag gcgacttttg aacgcgcaat
     7981 aatggtttct gacgtatgtg cttagctcat taaactccag aaacccgcgg ctcagtggct
     8041 ccttcaacgt tgcggttctg tcagttccaa acgtaaaacg gcttgtcccg cgtcatcggc
     8101 gggggtcata acgtgactcc cttaattctc cgctcatgat cgataattcg cggtacccgg
     8161 ggatcctcta gagggcccga cgtcgcatgc ctgcaggtca ctggattttg gttttaggaa
     8221 ttagaaattt tattgataga agtattttac aaatacaaat acatactaag ggtttcttat
     8281 atgctcaaca catgagcgaa accctataag aaccctaatt cccttatctg ggaactactc
     8341 acacattatt ctggagaaaa atagagagag atagatttgt agagagagac tggtgatttt
     8401 tgcggactct agcatggccg cttataaact agttggatat ctcgagtcga cacgcgtaga
     8461 tctccgcgga cgtcccatca ccactttgta caagaaagct gggttcagtt acctagatcc
     8521 ggtggatcct cacactgctt gcttcaaaac cttatcaaag tgaccatcca tcctggctct
     8581 tatagcctct gctaactgtt cataccttga tctcaatggt gaacctggcc tgtagaccaa
     8641 tccaatggtc cttcttggct ctggtttaat gcatggaaga taaactacac catccctctt
     8701 cctctctggt ggcacggcaa gtgctggtaa caaagttatt ccacttcctg cggctaccat
     8761 gttcctcaag gtttctagtg atgtagccct gaagtgagta tcctcgtcag caccggcttc
     8821 gaagcaaaat ccggttccgt tgtactctag cttgtgtcca agaatatttc cgtcctcttt
     8881 gaatccgata cctttaagct ctattctatt caccaaagta tctccctcaa acttcacttc
     8941 tgccctagtt ttataattac cgtcgtcttt gaagaatata gtcctctctt gcacatatcc
     9001 ctcaggcata gcactcttga agaagtcatg ttgcttcata tggtcaggat atcttgcgaa
     9061 gcactttaaa ccgtaaccta aggttgttac taatgttggc catgggacag gaagttttcc
     9121 agttgtacag attaatttca atgttagttt tccgtaggtt gcgtcaccct caccttctcc
     9181 ggagactgaa aatttgtgac cgttcacgtc accatcaagt tctactaata ttgggacaac
     9241 tcctgtgaac aactcttctc ccttggaacc tgtacctcca ctacctccgt ctacattgta
     9301 tagctcatcc ataccaagag taataccggc agcggttacg aactcaagca acaccatgtg
     9361 atcccttttc tcatttgggt ctttggatag gacagactga aaagataggt agtggttatc
     9421 tggaagtaaa acaggtccgt caccgattgg ggtgttctgt tgataatggt ctgctagttg
     9481 tacggaacca tcttccacgt tatgtctaat tttgaagttg gcctttatac cattcttctg
     9541 tttgtcggcc atgatataga cgttatctga attgtaaccg gcggacatag cttgatccct
     9601 caaggagtgt ccatcctcaa gcattagaag tttctcaccg gccaaatctg ccattggaac
     9661 gcattctcta ttggcccatg ggtgatcttc atagattgct aaaagcatag gttcatcgaa
     9721 aagaggcact tctatgaatg cctcggattc ctttaccaaa gctagtatga cacaatcaag
     9781 tttaccacta tctaactggg ccaatagttg atgagtctgt gcctcgtgaa ggtacatttc
     9841 aagtttaggg aaagtttggt gtagcattgg gataatgtga ggaagtaagt aaggtccaac
     9901 tgttgggatt agaccgatat gcaatggtcc tgacatagtc tcaccctgtt gagaagccat
     9961 ttccatggtg gcgaccggta gcgctagcag cctgcttttt tgtacaaact tgtgatgggc
    10021 cctctagagg atcccgcact cgagtgttaa tcagaaaaac tcagattaat ctacaaattc
    10081 gatcgcacaa actagaaact aacaccagat ctagatagaa atcacaaatc gaagagtaat
    10141 tattcgacaa aactcaaatt atttgaacaa atcggatgat atctatgaaa ccctaatcga
    10201 gaattaagat gatatctaac gatcaaaccc agaaaatcgt cttcgatcta agattaacag
    10261 aatctaaacc aaagaacata tacgaaattg ggatcgaacg aaaacaaaat cgaagatttt
    10321 gagagaataa ggaacacaga aatttacctt gatcacggta gagagaattg agagaaagtt
    10381 tttaagattt tgagaaattg aaatctgaat tgtgaagaag aagagctctt tgggtattgt
    10441 tttatagaag aagaagaaga aaagacgagg acgactaggt cacgagaaag ctaaggcggt
    10501 gaagcaatag ctaataataa aatgacacgt gtattgagcg ttgtttacac gcaaagttgt
    10561 ttttggctaa ttgccttatt tttaggttga ggaaaagtat ttgtgctttg agttgataaa
    10621 cacgactcgt gtgtgccggc tgcaacca
//
