LOCUS       sequence-180315-       10154 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses pHRed in plant cells-biosensor for pHRed targeted to 
            chloroplasts stoma.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10154)
  AUTHORS   Exposito-Rodriguez M, Laissue PP, Yvon-Durocher G, Smirnoff N, 
            Mullineaux PM
  TITLE     Photosynthesis-dependent H2O2 transfer from chloroplasts to nuclei 
            provides a high-light signalling mechanism.
  JOURNAL   Nat Commun. 2017 Jun 29;8(1):49. doi: 10.1038/s41467-017-00074-w.
  PUBMED    28663550
REFERENCE   2  (bases 1 to 10154)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2017-06-29"; volume: "8(1):49. doi"; pages: " 
            10.1038/s41467-017-00074-w"
COMMENT     Sequence Label: pUB-spHRed
FEATURES             Location/Qualifiers
     source          1..10154
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    66..90
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             1421..2050
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             2479..3552
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      3618..3812
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(4048..4070)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    4156..4296
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     4211..4230
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4311..4328)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4482..5070)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4562..4581)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(5316..6107)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_feature    6628..6652
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(6745..6997)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(7017..7568)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(7610..7789)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     protein_bind    8211..8235
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(join(8297..8956,8957..8959))
                     /codon_start=1
                     /product="red fluorescent protein that serves as a 
                     ratiometric pH sensor (Tantama et al., 2011)"
                     /label=pHRed
                     /note="contains an A213S mutation relative to the parental 
                     mKeima protein"
                     /translation="VSVIAKQMTYKVYMSGTVNGHYFEVEGDGKGKPYEGEQTVKLTVT
                     KGGPLPFAWDILSPQLQYGSIPFTKYPEDIPDYFKQSFPEGYTWERSMNFEDGAVCTVS
                     NDSSIQGNCFIYNVKISGENFPPNGPVMQKKTQGWEPSTERLFARDGMLIGNDYMALKL
                     EGGGHYLCEFKSTYKAKKPVRMPGRHEIDRKLDVTSHNRDYTSVEQCEISIARHSLLG"
     protein_bind    complement(9226..9250)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(9941..9957)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9941..9957)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9954..9976)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9965..9981
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9989..9995,9996..10013,10014..10019))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10034..10055
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 ggctagagca gcttgagctt ggatcagatt gtcgtttccc gccttcagtt taaactatca
       61 gtgtttgaca ggatatattg gcgggtaaac ctaagagaaa agagcgttta ttagaataat
      121 cggatattta aaagggcgtg aaaaggttta tccgttcgtc catttgtatg tgcatgccaa
      181 ccacagggtt cccctcggga tcaaagtact ttaaagtact ttaaagtact ttaaagtact
      241 ttgatccaac ccctccgctg ctatagtgca gtcggcttct gacgttcagt gcagccgtct
      301 tctgaaaacg acatgtcgca caagtcctaa gttacgcgac aggctgccgc cctgcccttt
      361 tcctggcgtt ttcttgtcgc gtgttttagt cgcataaagt agaatacttg cgactagaac
      421 cggagacatt acgccatgaa caagagcgcc gccgctggcc tgctgggcta tgcccgcgtc
      481 agcaccgacg accaggactt gaccaaccaa cgggccgaac tgcacgcggc cggctgcacc
      541 aagctgtttt ccgagaagat caccggcacc aggcgcgacc gcccggagct ggccaggatg
      601 cttgaccacc tacgccctgg cgacgttgtg acagtgacca ggctagaccg cctggcccgc
      661 agcacccgcg acctactgga cattgccgag cgcatccagg aggccggcgc gggcctgcgt
      721 agcctggcag agccgtgggc cgacaccacc acgccggccg gccgcatggt gttgaccgtg
      781 ttcgccggca ttgccgagtt cgagcgttcc ctaatcatcg accgcacccg gagcgggcgc
      841 gaggccgcca aggcccgagg cgtgaagttt ggcccccgcc ctaccctcac cccggcacag
      901 atcgcgcacg cccgcgagct gatcgaccag gaaggccgca ccgtgaaaga ggcggctgca
      961 ctgcttggcg tgcatcgctc gaccctgtac cgcgcacttg agcgcagcga ggaagtgacg
     1021 cccaccgagg ccaggcggcg cggtgccttc cgtgaggacg cattgaccga ggccgacgcc
     1081 ctggcggccg ccgagaatga acgccaagag gaacaagcat gaaaccgcac caggacggcc
     1141 aggacgaacc gtttttcatt accgaagaga tcgaggcgga gatgatcgcg gccgggtacg
     1201 tgttcgagcc gcccgcgcac gtctcaaccg tgcggctgca tgaaatcctg gccggtttgt
     1261 ctgatgccaa gctggcggcc tggccggcca gcttggccgc tgaagaaacc gagcgccgcc
     1321 gtctaaaaag gtgatgtgta tttgagtaaa acagcttgcg tcatgcggtc gctgcgtata
     1381 tgatgcgatg agtaaataaa caaatacgca aggggaacgc atgaaggtta tcgctgtact
     1441 taaccagaaa ggcgggtcag gcaagacgac catcgcaacc catctagccc gcgccctgca
     1501 actcgccggg gccgatgttc tgttagtcga ttccgatccc cagggcagtg cccgcgattg
     1561 ggcggccgtg cgggaagatc aaccgctaac cgttgtcggc atcgaccgcc cgacgattga
     1621 ccgcgacgtg aaggccatcg gccggcgcga cttcgtagtg atcgacggag cgccccaggc
     1681 ggcggacttg gctgtgtccg cgatcaaggc agccgacttc gtgctgattc cggtgcagcc
     1741 aagcccttac gacatatggg ccaccgccga cctggtggag ctggttaagc agcgcattga
     1801 ggtcacggat ggaaggctac aagcggcctt tgtcgtgtcg cgggcgatca aaggcacgcg
     1861 catcggcggt gaggttgccg aggcgctggc cgggtacgag ctgcccattc ttgagtcccg
     1921 tatcacgcag cgcgtgagct acccaggcac tgccgccgcc ggcacaaccg ttcttgaatc
     1981 agaacccgag ggcgacgctg cccgcgaggt ccaggcgctg gccgctgaaa ttaaatcaaa
     2041 actcatttga gttaatgagg taaagagaaa atgagcaaaa gcacaaacac gctaagtgcc
     2101 ggccgtccga gcgcacgcag cagcaaggct gcaacgttgg ccagcctggc agacacgcca
     2161 gccatgaagc gggtcaactt tcagttgccg gcggaggatc acaccaagct gaagatgtac
     2221 gcggtacgcc aaggcaagac cattaccgag ctgctatctg aatacatcgc gcagctacca
     2281 gagtaaatga gcaaatgaat aaatgagtag atgaatttta gcggctaaag gaggcggcat
     2341 ggaaaatcaa gaacaaccag gcaccgacgc cgtggaatgc cccatgtgtg gaggaacggg
     2401 cggttggcca ggcgtaagcg gctgggttgt ctgccggccc tgcaatggca ctggaacccc
     2461 caagcccgag gaatcggcgt gagcggtcgc aaaccatccg gcccggtaca aatcggcgcg
     2521 gcgctgggtg atgacctggt ggagaagttg aaggccgcgc aggccgccca gcggcaacgc
     2581 atcgaggcag aagcacgccc cggtgaatcg tggcaagcgg ccgctgatcg aatccgcaaa
     2641 gaatcccggc aaccgccggc agccggtgcg ccgtcgatta ggaagccgcc caagggcgac
     2701 gagcaaccag attttttcgt tccgatgctc tatgacgtgg gcacccgcga tagtcgcagc
     2761 atcatggacg tggccgtttt ccgtctgtcg aagcgtgacc gacgagctgg cgaggtgatc
     2821 cgctacgagc ttccagacgg gcacgtagag gtttccgcag ggccggccgg catggccagt
     2881 gtgtgggatt acgacctggt actgatggcg gtttcccatc taaccgaatc catgaaccga
     2941 taccgggaag ggaagggaga caagcccggc cgcgtgttcc gtccacacgt tgcggacgta
     3001 ctcaagttct gccggcgagc cgatggcgga aagcagaaag acgacctggt agaaacctgc
     3061 attcggttaa acaccacgca cgttgccatg cagcgtacga agaaggccaa gaacggccgc
     3121 ctggtgacgg tatccgaggg tgaagccttg attagccgct acaagatcgt aaagagcgaa
     3181 accgggcggc cggagtacat cgagatcgag ctagctgatt ggatgtaccg cgagatcaca
     3241 gaaggcaaga acccggacgt gctgacggtt caccccgatt actttttgat cgatcccggc
     3301 atcggccgtt ttctctaccg cctggcacgc cgcgccgcag gcaaggcaga agccagatgg
     3361 ttgttcaaga cgatctacga acgcagtggc agcgccggag agttcaagaa gttctgtttc
     3421 accgtgcgca agctgatcgg gtcaaatgac ctgccggagt acgatttgaa ggaggaggcg
     3481 gggcaggctg gcccgatcct agtcatgcgc taccgcaacc tgatcgaggg cgaagcatcc
     3541 gccggttcct aatgtacgga gcagatgcta gggcaaattg ccctagcagg ggaaaaaggt
     3601 cgaaaaggtc tctttcctgt ggatagcacg tacattggga acccaaagcc gtacattggg
     3661 aaccggaacc cgtacattgg gaacccaaag ccgtacattg ggaaccggtc acacatgtaa
     3721 gtgactgata taaaagagaa aaaaggcgat ttttccgcct aaaactcttt aaaacttatt
     3781 aaaactctta aaacccgcct ggcctgtgca taactgtctg gccagcgcac agccgaagag
     3841 ctgcaaaaag cgcctaccct tcggtcgctg cgctccctac gccccgccgc ttcgcgtcgg
     3901 cctatcgcgg ccgctggccg ctcaaaaatg gctggcctac ggccaggcaa tctaccaggg
     3961 cgcggacaag ccgcgccgtc gccactcgac cgccggcgcc cacatcaagg caccctgcct
     4021 cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac
     4081 agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt
     4141 tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc gatagcggag tgtatactgg
     4201 cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata
     4261 ccgcacagat gcgtaaggag aaaataccgc atcaggcgct cttccgcttc ctcgctcact
     4321 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     4381 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     4441 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     4501 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     4561 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     4621 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     4681 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     4741 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     4801 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     4861 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     4921 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     4981 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     5041 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     5101 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgcatga tatatctccc
     5161 aatttgtgta gggcttatta tgcacgctta aaaataataa aagcagactt gacctgatag
     5221 tttggctgtg agcaattatg tgcttagtgc atctaacgct tgagttaagc cgcgccgcga
     5281 agcggcgtcg gcttgaacga atttctagct agacattatt tgccgactac cttggtgatc
     5341 tcgcctttca cgtagtggac aaattcttcc aactgatctg cgcgcgaggc caagcgatct
     5401 tcttcttgtc caagataagc ctgtctagct tcaagtatga cgggctgata ctgggccggc
     5461 aggcgctcca ttgcccagtc ggcagcgaca tccttcggcg cgattttgcc ggttactgcg
     5521 ctgtaccaaa tgcgggacaa cgtaagcact acatttcgct catcgccagc ccagtcgggc
     5581 ggcgagttcc atagcgttaa ggtttcattt agcgcctcaa atagatcctg ttcaggaacc
     5641 ggatcaaaga gttcctccgc cgctggacct accaaggcaa cgctatgttc tcttgctttt
     5701 gtcagcaaga tagccagatc aatgtcgatc gtggctggct cgaagatacc tgcaagaatg
     5761 tcattgcgct gccattctcc aaattgcagt tcgcgcttag ctggataacg ccacggaatg
     5821 atgtcgtcgt gcacaacaat ggtgacttct acagcgcgga gaatctcgct ctctccaggg
     5881 gaagccgaag tttccaaaag gtcgttgatc aaagctcgcc gcgttgtttc atcaagcctt
     5941 acggtcaccg taaccagcaa atcaatatca ctgtgtggct tcaggccgcc atccactgcg
     6001 gagccgtaca aatgtacggc cagcaacgtc ggttcgagat ggcgctcgat gacgccaact
     6061 acctctgata gttgagtcga tacttcggcg atcaccgctt cccccatgat gtttaacttt
     6121 gttttagggc gactgccctg ctgcgtaaca tcgttgctgc tccataacat caaacatcga
     6181 cccacggcgt aacgcgcttg ctgcttggat gcccgaggca tagactgtac cccaaaaaaa
     6241 cagtcataac aagccatgaa aaccgccact gcgccgttac caccgctgcg ttcggtcaag
     6301 gttctggacc agttgcgtga gcgcatacgc tacttgcatt acagcttacg aaccgaacag
     6361 gcttatgtcc actgggttcg tgcccgaatt gatcacaggc agcaacgctc tgtcatcgtt
     6421 acaatcaaca tgctaccctc cgcgagatca tccgtgtttc aaacccggca gcttagttgc
     6481 cgttcttccg aatagcatcg gtaacatgag caaagtctgc cgccttacaa cggctctccc
     6541 gctgacgccg tcccggactg atgggctgcc tgtatcgagt ggtgattttg tgccgagctg
     6601 ccggtcgggg agctgttggc tggctggtgg caggatatat tgtggtgtaa acaaattgac
     6661 gcttagacaa cttaataaca cattgcggac gtttttaatg tactgaatta acgccgaatt
     6721 gaattatcag cttgcatgcc ggtcgatcta gtaacataga tgacaccgcg cgcgataatt
     6781 tatcctagtt tgcgcgctat attttgtttt ctatcgcgta ttaaatgtat aattgcggga
     6841 ctctaatcat aaaaacccat ctcataaata acgtcatgca ttacatgtta attattacat
     6901 gcttaacgta attcaacaga aattatatga taatcatcgc aagaccggca acaggattca
     6961 atcttaagaa actttattgc caaatgtttg aacgatctgc ttgactctag gggtcatcag
     7021 atttcggtga cgggcaggac cggacggggc ggcaccggca ggctgaagtc cagctgccag
     7081 aaacccacgt catgccagtt cccgtgcttg aagccggccg cccgcagcat gccgcggggg
     7141 gcatatccga gcgcctcgtg catgcgcacg ctcgggtcgt tgggcagccc gatgacagcg
     7201 accacgctct tgaagccctg tgcctccagg gacttcagca ggtgggtgta gagcgtggag
     7261 cccagtcccg tccgctggtg gcggggggag acgtacacgg tcgactcggc cgtccagtcg
     7321 taggcgttgc gtgccttcca gggacccgcg taggcgatgc cggcgacctc gccgtccacc
     7381 tcggcgacga gccagggata gcgctcccgc agacggacga ggtcgtccgt ccactcctgc
     7441 ggttcctgcg gctcggtacg gaagttgacc gtgcttgtct cgatgtagtg gttgacgatg
     7501 gtgcagaccg ccggcatgtc cgcctcggtg gcacggcgga tgtcggccgg gcgtcgttct
     7561 gggctcatgg tagatcccct cgatcgagtt gagagtgaat atgagactct aattggatac
     7621 cgaggggaat ttatggaacg tcagtggagc atttttgaca agaaatattt gctagctgat
     7681 agtgacctta ggcgactttt gaacgcgcaa taatggtttc tgacgtatgt gcttagctca
     7741 ttaaactcca gaaacccgcg gctcagtggc tccttcaacg ttgcggttct gtcagttcca
     7801 aacgtaaaac ggcttgtccc gcgtcatcgg cgggggtcat aacgtgactc ccttaattct
     7861 ccgctcatga tcgataattc gcggtacccg gggatcctct agagggcccg acgtcgcatg
     7921 cctgcaggtc actggatttt ggttttagga attagaaatt ttattgatag aagtatttta
     7981 caaatacaaa tacatactaa gggtttctta tatgctcaac acatgagcga aaccctataa
     8041 gaaccctaat tcccttatct gggaactact cacacattat tctggagaaa aatagagaga
     8101 gatagatttg tagagagaga ctggtgattt ttgcggactc tagcatggcc gcttataaac
     8161 tagttggata tctcgagtcg acacgcgtag atctccgcgg acgtcccatc accactttgt
     8221 acaagaaagc tgggtcggcg cgcccaccct ttcatctaga accaccagag ccaccaccgt
     8281 cggcggagtt gcctgtcccc agcagagagt ggcgggcgat agagatttca cactgctcaa
     8341 cgctggtgta gtcgcggttg tgagaggtaa catccagctt gcggtcgatt tcgtggcggc
     8401 caggcatgcg aacaggcttt ttagccttgt aagtgctctt gaactcgcac aggtagtgac
     8461 cgccgccttc cagtttcagg gccatataat cgttaccaat cagcataccg tcgcgggcaa
     8521 acaggcgttc ggtgctgggc tcccagcctt gggttttctt ctgcataact gggccgttag
     8581 gtgggaagtt ctcgccggaa atcttaacgt tgtagatgaa gcagttaccc tggatggagc
     8641 tgtcgttgga aacggtacaa actgcgccgt cctcgaagtt catagagcgc tcccaggtgt
     8701 agccctctgg gaagctctgc ttaaagtagt cagggatgtc ctcggggtat ttggtgaaag
     8761 ggatggagcc gtattggagc tgaggggaca ggatatccca ggcgaaaggc agtgggccgc
     8821 ctttggtaac ggtcagctta acggtctgct cgccctcgta aggcttgccc ttgccgtcac
     8881 cttcaacctc gaagtagtgg ccgttaacgg tgccagacat ataaacttta taggtcattt
     8941 gctttgcgat aacggaaaca agcttttcgg aatcggtaag gtcaggaagg taagagagag
     9001 tctcaaactt cttctttcca atcggaggcc acacctgcat gcagttaact cttccgccgt
     9061 tgcttgtgat ggaagtaatg tcgttgttag ccttgcgggt ggctgggaag gcagcggagg
     9121 acttaagtcc gttgaaagga gcgaccatag tggcctgagc cggagaggca accatagtag
     9181 cggaagagag catagaggaa gccatggtga agggggcggc cgcggagcct gcttttttgt
     9241 acaaacttgt gatgggccct ctagaggatc ccgcactcga gtgttaatca gaaaaactca
     9301 gattaatcta caaattcgat cgcacaaact agaaactaac accagatcta gatagaaatc
     9361 acaaatcgaa gagtaattat tcgacaaaac tcaaattatt tgaacaaatc ggatgatatc
     9421 tatgaaaccc taatcgagaa ttaagatgat atctaacgat caaacccaga aaatcgtctt
     9481 cgatctaaga ttaacagaat ctaaaccaaa gaacatatac gaaattggga tcgaacgaaa
     9541 acaaaatcga agattttgag agaataagga acacagaaat ttaccttgat cacggtagag
     9601 agaattgaga gaaagttttt aagattttga gaaattgaaa tctgaattgt gaagaagaag
     9661 agctctttgg gtattgtttt atagaagaag aagaagaaaa gacgaggacg actaggtcac
     9721 gagaaagcta aggcggtgaa gcaatagcta ataataaaat gacacgtgta ttgagcgttg
     9781 tttacacgca aagttgtttt tggctaattg ccttattttt aggttgagga aaagtatttg
     9841 tgctttgagt tgataaacac gactcgtgtg tgccggctgc aaccactttg acgccgttta
     9901 ttactgactc gtcgggtacc gagctcgaat tcgtaatcat gtcatagctg tttcctgtgt
     9961 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
    10021 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
    10081 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
    10141 gcggtttgcg tatt
//
