LOCUS       sequence-180316-       19265 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Simultaneous expresion of stromal HyPer2 and stromal Ascorbate 
            peroxidase (APX) in plant cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 19265)
  AUTHORS   Exposito-Rodriguez M, Laissue PP, Yvon-Durocher G, Smirnoff N, 
            Mullineaux PM
  TITLE     Photosynthesis-dependent H2O2 transfer from chloroplasts to nuclei 
            provides a high-light signalling mechanism.
  JOURNAL   Nat Commun. 2017 Jun 29;8(1):49. doi: 10.1038/s41467-017-00074-w.
  PUBMED    28663550
REFERENCE   2  (bases 1 to 19265)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2017-06-29"; volume: "8(1):49. doi"; pages: " 
            10.1038/s41467-017-00074-w"
COMMENT     Sequence Label: pGem-nHyPer:sAPX
FEATURES             Location/Qualifiers
     source          1..19265
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    56..80
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1236..1260)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      1292..1544
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      complement(1973..2225)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(2693..3718)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(2693..3718)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(3728..4072)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(4214..4231)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4214..4230)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4223..4245)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(4370..4389)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4580..4601
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    4848..4872
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(6468..7616)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(7915..8709)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  8901..9668
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      10291..11002
                     /label=oriV
                     /note="incP origin of replication"
     CDS             complement(11129..11500)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            11533..11642
                     /note="incP origin of transfer"
     primer_bind     11865..11884
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12118..12135
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(12323..12973)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    13438..13462
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        13586..13765
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        13618..13801
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             13822..14616
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(13876..13895)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     14486..14505
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      15006..15258
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    15807..15828
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(15843..15848,15849..15866,15867..15873)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    15881..15897
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     15886..15908
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     15905..15921
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     15905..15921
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     terminator      complement(15952..16204)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    16279..16303
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(16323..16343)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(17775..17795)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    complement(17819..17843)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        join(18954..19265,1..34)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
ORIGIN
        1 tatataagga agttcatttc atttggagag aacacggggg actctagagt tatcaacaag
       61 tttgtacaaa aaagcaggct ccgcggccgc ccccttcacc atggcagagc gtgtgtctct
      121 cacactcaac ggaaccctcc tttctcctcc tcccacaaca acaacaacaa caatgtcttc
      181 ttctctccga tctaccaccg ccgcttctct tctcctccgc tcctcctcct cctcctccag
      241 atccactctc actctctccg cttcctcctc tctctccttc gtcagatctc tcgtctcttc
      301 tccacgtctc tcttcttctt catccctctc tcagaagaaa tatcgaattg cgtcggtgaa
      361 tcggagtttc aactccacca ccgccgctac taaatcttca tcttcagatc ctgatcagtt
      421 gaagaacgct agagaagata tcaaagagct tctcagcacc aaattctgcc atccgatttt
      481 ggttagatta ggttggcatg atgctggtac gtataacaag aacattaagg aatggccaca
      541 aagaggtgga gctaatggaa gtttgagatt cgatattgag cttaaacatg ctgctaacgc
      601 tggtcttgtg aatgctttaa acttgattaa ggatatcaaa gagaaatact ctgggatcag
      661 ttatgctgat ttgttccagt tagctagtgc tactgctata gaggaagctg gaggaccgaa
      721 aataccgatg aaatatggta gagttgatgc ctctggtcct gaggattgtc cagaagaagg
      781 aaggcttcca gatgctggtc ctccttcacc tgctactcat ctcagagaag ttttttacag
      841 aatgggatta gatgacaagg acatagttgc attatctggt gctcacacgt taggaagatc
      901 taggccagaa cgtagtggtt gggggaagcc tgagacgaag tacacgaaag aaggacctgg
      961 agcaccagga ggacagtcat ggacaccaga gtggctgaag tttgataatt cttacttcaa
     1021 ggagatcaag gaaaagagag atgaagatct ccttgtccta cccactgatg ctgccatctt
     1081 cgaagattct tctttcaagg tctatgctga aaagtatgct gcagatcagg atgcattttt
     1141 caaggattac gctgtagccc atgcgaagct cagcaatctt ggagcagaat ttaaccctcc
     1201 ggagggtatc gttatctaaa agggtgggcg cgccgaccca gctttcttgt acaaagtggt
     1261 tgataacagc gcttagagct cgaatttccc cgatcgttca aacatttggc aataaagttt
     1321 cttaagattg aatcctgttg ccggtcttgc gatgattatc atataatttc tgttgaatta
     1381 cgttaagcat gtaataatta acatgtaatg catgacgtta tttatgagat gggtttttat
     1441 gattagagtc ccgcaattat acatttaata cgcgatagaa aacaaaatat agcgcgcaaa
     1501 ctaggataaa ttatcgcgcg cggtgtcatc tatgttacta gatcgggaat tagcttcatc
     1561 aacgcaagac atgcgcacga ccgtctgaca ggagaggaat ttccgacgag cacagaaagg
     1621 acttgctctt ggacgtaggc ctatttctca ggcacatgta tcaagtgttc ggacgtgggt
     1681 tttcgatggt gtatcagccg ccgccaactg ggagatgagg aggctttctt ggggggcagt
     1741 cagcagttca tttcacaaga cagaggaact tgtaaggaga tgcactgatt tatcttggcg
     1801 caaaccagca ggacgaatta gtgggaatag cccgcgaata tctaagttat gcctgtcggc
     1861 atgagcagaa acttccaatt cgaaacagtt tggagaggtt gtttttgggc ataccttttg
     1921 ttagtcagcc tctcgattgc tcatcgtcat tacacagtac cgaagtttga tcgatctagt
     1981 aacatagatg acaccgcgcg cgataattta tcctagtttg cgcgctatat tttgttttct
     2041 atcgcgtatt aaatgtataa ttgcgggact ctaatcataa aaacccatct cataaataac
     2101 gtcatgcatt acatgttaat tattacatgc ttaacgtaat tcaacagaaa ttatatgata
     2161 atcatcgcaa gaccggcaac aggattcaat cttaagaaac tttattgcca aatgtttgaa
     2221 cgatctgctt cgacgcactc cttctttact ccaccatctc gtccttattg aaaacgtggg
     2281 tagcaccaaa acgaatcaag tcgctggaac tgaagttacc aatcacgctg gatgatttgc
     2341 cagttggatt aatcttgcct ttccccgcat gaataatatt gatgaatgca tgcgtgaggg
     2401 gtatttcgat tttggcaata gctgcaattg ccgcgacatc ctccaacgag cataattctt
     2461 cagaaaaata gcgatgttcc atgttgtcag ggcatgcatg atgcacgtta tgaggtgacg
     2521 gtgctaggca gtattccctc aaagtttcat agtcagtatc atattcatca ttgcattcct
     2581 gcaagagaga attgagacgc aatccacacg ctgcggcaac cttccggcgt tcgtggtcta
     2641 tttgctcttg gacgttgcaa acgtaagtgt tggatcccgg tcggcatcta ctctattcct
     2701 ttgccctcgg acgagtgctg gggcgtcggt ttccactatc ggcgagtact tctacacagc
     2761 catcggtcca gacggccgcg cttctgcggg cgatttgtgt acgcccgaca gtcccggctc
     2821 cggatcggac gattgcgtcg catcgaccct gcgcccaagc tgcatcatcg aaattgccgt
     2881 caaccaagct ctgatagagt tggtcaagac caatgcggag catatacgcc cggagccgcg
     2941 gcgatcctgc aagctccgga tgcctccgct cgaagtagcg cgtctgctgc tccatacaag
     3001 ccaaccacgg cctccagaag aagatgttgg cgacctcgta ttgggaatcc ccgaacatcg
     3061 cctcgctcca gtcaatgacc gctgttatgc ggccattgtc cgtcaggaca ttgttggagc
     3121 cgaaatccgc gtgcacgagg tgccggactt cggggcagtc ctcggcccaa agcatcagct
     3181 catcgagagc ctgcgcgacg gacgcactga cggtgtcgtc catcacagtt tgccagtgat
     3241 acacatgggg atcagcaatc gcgcatatga aatcacgcca tgtagtgtat tgaccgattc
     3301 cttgcggtcc gaatgggccg aacccgctcg tctggctaag atcggccgca gcgatcgcat
     3361 ccatggcctc cgcgaccggc tgcagaacag cgggcagttc ggtttcaggc aggtcttgca
     3421 acgtgacacc ctgtgcacgg cgggagatgc aataggtcag gctctcgctg aattccccaa
     3481 tgtcaagcac ttccggaatc gggagcgcgg ccgatgcaaa gtgccgataa acataacgat
     3541 ctttgtagaa accatcggcg cagctattta cccgcaggac atatccacgc cctcctacat
     3601 cgaagctgaa agcacgagat tcttcgccct ccgagagctg catcaggtcg gagacgctgt
     3661 cgaacttttc gatcagaaac ttctcgacag acgtcgcggt gagttcaggc tttttcatat
     3721 cggggtcgtc ctctccaaat gaaatgaact tccttatata gaggaagggt cttgcgaagg
     3781 atagtgggat tgtgcgtcat cccttacgtc agtggagata tcacatcaat ccacttgctt
     3841 tgaagacgtg gttggaacgt cttctttttc cacgatgctc ctcgtgggtg ggggtccatc
     3901 tttgggacca ctgtcggcag aggcatcttg aacgatagcc tttcctttat cgcaatgatg
     3961 gcatttgtag gtgccacctt ccttttctac tgtccttttg atgaagtgac agatagctgg
     4021 gcaatggaat ccgaggaggt ttcccgatat taccctttgt tgaaaagtct caatagccct
     4081 ttggtcttct gagactgtat ctttgatatt cttggagtag acgagagtgt cgtgctccac
     4141 catgttgacg gatctctagg acgcgtcgac gcgtcgacgc gtcgacgcgt cgacgcgtcc
     4201 tagaagctaa ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta
     4261 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg
     4321 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgcccgc tcctttcgct
     4381 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     4441 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgatttg
     4501 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     4561 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
     4621 tcgggctatt cttttgattt ataagggatt ttgccgattt cggaaccacc atcaaacagg
     4681 attttcgcct gctggggcaa accagcgtgg accgcttgct gcaactctct cagggccagg
     4741 cggtgaaggg caatcagctg ttgcccgtct cactggtgaa aagaaaaacc accccagtac
     4801 attaaaaacg tccgcaatgt gttattaagt tgtctaagcg tcaatttgtt tacaccacaa
     4861 tatatcctgc caccagccag ccaacagctc cccgaccggc agctcggcac aaaatcacca
     4921 ctcgatacag gcagcccatc agtccgggac ggcgtcagcg ggagagccgt tgtaaggcgg
     4981 cagactttgc tcatgttacc gatgctattc ggaagaacgg caactaagct gccgggtttg
     5041 aaacacggat gatctcgcgg agggtagcat gttgattgta acgatgacag agcgttgctg
     5101 cctgtgatca aatatcatct ccctcgcaga gatccgaatt atcagccttc ttattcattt
     5161 ctcgcttaac cgtgacaggc tgtcgatctt gagaactatg ccgacataat aggaaatcgc
     5221 tggataaagc cgctgaggaa gctgagtggc gctatttctt tagaagtgaa cgttgacgat
     5281 atcaactccc ctatccattg ctcaccgaat ggtacaggtc ggggacccga agttccgact
     5341 gtcggcctga tgcatccccg gctgatcgac cccagatctg gggctgagaa agcccagtaa
     5401 ggaaacaact gtaggttcga gtcgcgagat cccccggaac caaaggaagt aggttaaacc
     5461 cgctccgatc aggccgagcc acgccaggcc gagaacattg gttcctgtag gcatcgggat
     5521 tggcggatca aacactaaag ctactggaac gagcagaagt cctccggccg ccagttgcca
     5581 ggcggtaaag gtgagcagag gcacgggagg ttgccacttg cgggtcagca cggttccgaa
     5641 cgccatggaa accgcccccg ccaggcccgc tgcgacgccg acaggatcta gcgctgcgtt
     5701 tggtgtcaac accaacagcg ccacgcccgc agttccgcaa atagccccca ggaccgccat
     5761 caatcgtatc gggctaccta gcagagcggc agagatgaac acgaccatca gcggctgcac
     5821 agcgcctacc gtcgccgcga ccccgcccgg caggcggtag accgaaataa acaacaagct
     5881 ccagaatagc gaaatattaa gtgcgccgag gatgaagatg cgcatccacc agattcccgt
     5941 tggaatctgt cggacgatca tcacgagcaa taaacccgcc ggcaacgccc gcagcagcat
     6001 accggcgacc cctcggcctc gctgttcggg ctccacgaaa acgccggaca gatgcgcctt
     6061 gtgagcgtcc ttggggccgt cctcctgttt gaagaccgac agcccaatga tctcgccgtc
     6121 gatgtaggcg ccgaatgcca cggcatctcg caaccgttca gcgaacgcct ccatgggctt
     6181 tttctcctcg tgctcgtaaa cggacccgaa catctctgga gctttcttca gggccgacaa
     6241 tcggatctcg cggaaatcct gcacgtcggc cgctccaagc cgtcgaatct gagccttaat
     6301 cacaattgtc aattttaatc ctctgtttat cggcagttcg tagagcgcgc cgtgcgtccc
     6361 gagcgatact gagcgaagca agtgcgtcga gcagtgcccg cttgttcctg aaatgccagt
     6421 aaagcgctgg ctgctgaacc cccagccgga actgacccca caaggcccta gcgtttgcaa
     6481 tgcaccaggt catcattgac ccaggcgtgt tccaccaggc cgctgcctcg caactcttcg
     6541 caggcttcgc cgacctgctc gcgccacttc ttcacgcggg tggaatccga tccgcacatg
     6601 aggcggaagg tttccagctt gagcgggtac ggctcccggt gcgagctgaa atagtcgaac
     6661 atccgtcggg ccgtcggcga cagcttgcgg tacttctccc atatgaattt cgtgtagtgg
     6721 tcgccagcaa acagcacgac gatttcctcg tcgatcagga cctggcaacg ggacgttttc
     6781 ttgccacggt ccaggacgcg gaagcggtgc agcagcgaca ccgattccag gtgcccaacg
     6841 cggtcggacg tgaagcccat cgccgtcgcc tgtaggcgcg acaggcattc ctcggccttc
     6901 gtgtaatacc ggccattgat cgaccagccc aggtcctggc aaagctcgta gaacgtgaag
     6961 gtgatcggct cgccgatagg ggtgcgcttc gcgtactcca acacctgctg ccacaccagt
     7021 tcgtcatcgt cggcccgcag ctcgacgccg gtgtaggtga tcttcacgtc cttgttgacg
     7081 tggaaaatga ccttgttttg cagcgcctcg cgcgggattt tcttgttgcg cgtggtgaac
     7141 agggcagagc gggccgtgtc gtttggcatc gctcgcatcg tgtccggcca cggcgcaata
     7201 tcgaacaagg aaagctgcat ttccttgatc tgctgcttcg tgtgtttcag caacgcggcc
     7261 tgcttggcct cgctgacctg ttttgccagg tcctcgccgg cggtttttcg cttcttggtc
     7321 gtcatagttc ctcgcgtgtc gatggtcatc gacttcgcca aacctgccgc ctcctgttcg
     7381 agacgacgcg aacgctccac ggcggccgat ggcgcgggca gggcaggggg agccagttgc
     7441 acgctgtcgc gctcgatctt ggccgtagct tgctggacca tcgagccgac ggactggaag
     7501 gtttcgcggg gcgcacgcat gacggtgcgg cttgcgatgg tttcggcatc ctcggcggaa
     7561 aaccccgcgt cgatcagttc ttgcctgtat gccttccggt caaacgtccg attcattcac
     7621 cctccttgcg ggattgcccc gactcacgcc ggggcaatgt gcccttattc ctgatttgac
     7681 ccgcctggtg ccttggtgtc cagataatcc accttatcgg caatgaagtc ggtcccgtag
     7741 accgtctggc cgtccttctc gtacttggta ttccgaatct tgccctgcac gaataccagc
     7801 gaccccttgc ccaaatactt gccgtgggcc tcggcctgag agccaaaaca cttgatgcgg
     7861 aagaagtcgg tgcgctcctg cttgtcgccg gcatcgttgc gccacatcta ggtactaaaa
     7921 caattcatcc agtaaaatat aatattttat tttctcccaa tcaggcttga tccccagtaa
     7981 gtcaaaaaat agctcgacat actgttcttc cccgatatcc tccctgatcg accggacgca
     8041 gaaggcaatg tcataccact tgtccgccct gccgcttctc ccaagatcaa taaagccact
     8101 tactttgcca tctttcacaa agatgttgct gtctcccagg tcgccgtggg aaaagacaag
     8161 ttcctcttcg ggcttttccg tctttaaaaa atcatacagc tcgcgcggat ctttaaatgg
     8221 agtgtcttct tcccagtttt cgcaatccac atcggccaga tcgttattca gtaagtaatc
     8281 caattcggct aagcggctgt ctaagctatt cgtataggga caatccgata tgtcgatgga
     8341 gtgaaagagc ctgatgcact ccgcatacag ctcgataatc ttttcagggc tttgttcatc
     8401 ttcatactct tccgagcaaa ggacgccatc ggcctcactc atgagcagat tgctccagcc
     8461 atcatgccgt tcaaagtgca ggacctttgg aacaggcagc tttccttcca gccatagcat
     8521 catgtccttt tcccgttcca catcataggt ggtcccttta taccggctgt ccgtcatttt
     8581 taaatatagg ttttcatttt ctcccaccag cttatatacc ttagcaggag acattccttc
     8641 cgtatctttt acgcagcggt atttttcgat cagttttttc aattccggtg atattctcat
     8701 tttagccatt tattatttcc ttcctctttt ctacagtatt taaagatacc ccaagaagct
     8761 aattataaca agacgaactc caattcactg ttccttgcat tctaaaacct taaataccag
     8821 aaaacagctt tttcaaagtt gttttcaaag ttggcgtata acatagtatc gacggagccg
     8881 attttgaaac cacaattatg ggtgatgctg ccaacttact gatttagtgt atgatggtgt
     8941 ttttgaggtg ctccagtggc ttctgtttct atcagctgtc cctcctgttc agctactgac
     9001 ggggtggtgc gtaacggcaa aagcaccgcc ggacatcagc gctatctctg ctctcactgc
     9061 cgtaaaacat ggcaactgca gttcacttac accgcttctc aacccggtac gcaccagaaa
     9121 atcattgata tggccatgaa tggcgttgga tgccgggcaa cagcccgcat tatgggcgtt
     9181 ggcctcaaca cgattttacg tcacttaaaa aactcaggcc gcagtcggta acctcgcgca
     9241 tacagccggg cagtgacgtc atcgtctgcg cggaaatgga cgaacagtgg ggctatgtcg
     9301 gggctaaatc gcgccagcgc tggctgtttt acgcgtatga cagtctccgg aagacggttg
     9361 ttgcgcacgt attcggtgaa cgcactatgg cgacgctggg gcgtcttatg agcctgctgt
     9421 caccctttga cgtggtgata tggatgacgg atggctggcc gctgtatgaa tcccgcctga
     9481 agggaaagct gcacgtaatc agcaagcgat atacgcagcg aattgagcgg cataacctga
     9541 atctgaggca gcacctggca cggctgggac ggaagtcgct gtcgttctca aaatcggtgg
     9601 agctgcatga caaagtcatc gggcattatc tgaacataaa acactatcaa taagttggag
     9661 tcattaccca attatgatag aatttacaag ctataaggtt attgtcctgg gtttcaagca
     9721 ttagtccatg caagttttta tgctttgccc attctataga tatattgata agcgcgctgc
     9781 ctatgccttg ccccctgaaa tccttacata cggcgatatc ttctatataa aagatatatt
     9841 atcttatcag tattgtcaat atattcaagg caatctgcct cctcatcctc ttcatcctct
     9901 tcgtcttggt agctttttaa atatggcgct tcatagagta attctgtaaa ggtccaattc
     9961 tcgttttcat acctcggtat aatcttacct atcacctcaa atggttcgct gggtttatcg
    10021 cacccccgaa cacgagcacg gcacccgcga ccactatgcc aagaatgccc aaggtaaaaa
    10081 ttgccggccc cgccatgaag tccgtgaatg ccccgacggc cgaagtgaag ggcaggccgc
    10141 cacccaggcc gccgccctca ctgcccggca cctggtcgct gaatgtcgat gccagcacct
    10201 gcggcacgtc aatgcttccg ggcgtcgcgc tcgggctgat cgcccatccc gttactgccc
    10261 cgatcccggc aatggcaagg actgccagcg ctgccatttt tggggtgagg ccgttcgcgg
    10321 ccgaggggcg cagcccctgg ggggatggga ggcccgcgtt agcgggccgg gagggttcga
    10381 gaaggggggg cacccccctt cggcgtgcgc ggtcacgcgc acagggcgca gccctggtta
    10441 aaaacaaggt ttataaatat tggtttaaaa gcaggttaaa agacaggtta gcggtggccg
    10501 aaaaacgggc ggaaaccctt gcaaatgctg gattttctgc ctgtggacag cccctcaaat
    10561 gtcaataggt gcgcccctca tctgtcagca ctctgcccct caagtgtcaa ggatcgcgcc
    10621 cctcatctgt cagtagtcgc gcccctcaag tgtcaatacc gcagggcact tatccccagg
    10681 cttgtccaca tcatctgtgg gaaactcgcg taaaatcagg cgttttcgcc gatttgcgag
    10741 gctggccagc tccacgtcgc cggccgaaat cgagcctgcc cctcatctgt caacgccgcg
    10801 ccgggtgagt cggcccctca agtgtcaacg tccgcccctc atctgtcagt gagggccaag
    10861 ttttccgcga ggtatccaca acgccggcgg ccgcggtgtc tcgcacacgg cttcgacggc
    10921 gtttctggcg cgtttgcagg gccatagacg gccgccagcc cagcggcgag ggcaaccagc
    10981 ccggtgagcg tcggaaaggc gctcggtctt gccttgctcg tcggtgatgt acttcaccag
    11041 ctccgcgaag tcgctcttct tgatggagcg catggggacg tgcttggcaa tcacgcgcac
    11101 cccccggccg ttttagcggc taaaaaagtc atggctctgc cctcgggcgg accacgccca
    11161 tcatgacctt gccaagctcg tcctgcttct cttcgatctt cgccagcagg gcgaggatcg
    11221 tggcatcacc gaaccgcgcc gtgcgcgggt cgtcggtgag ccagagtttc agcaggccgc
    11281 ccaggcggcc caggtcgcca ttgatgcggg ccagctcgcg gacgtgctca tagtccacga
    11341 cgcccgtgat tttgtagccc tggccgacgg ccagcaggta ggccgacagg ctcatgccgg
    11401 ccgccgccgc cttttcctca atcgctcttc gttcgtctgg aaggcagtac accttgatag
    11461 gtgggctgcc cttcctggtt ggcttggttt catcagccat ccgcttgccc tcatctgtta
    11521 cgccggcggt agccggccag cctcgcagag caggattccc gttgagcacc gccaggtgcg
    11581 aataagggac agtgaagaag gaacacccgc tcgcgggtgg gcctacttca cctatcctgc
    11641 ccggctgacg ccgttggata caccaaggaa agtctacacg aaccctttgg caaaatcctg
    11701 tatatcgtgc gaaaaaggat ggatataccg aaaaaatcgc tataatgacc ccgaagcagg
    11761 gttatgcagc ggaaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
    11821 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
    11881 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
    11941 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
    12001 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
    12061 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
    12121 gagtcagtga gcgaggaagc ggaagagcgc cagaaggccg ccagagaggc cgagcgcggc
    12181 cgtgaggctt ggacgctagg gcagggcatg aaaaagcccg tagcgggctg ctacgggcgt
    12241 ctgacgcggt ggaaaggggg aggggatgtt gtctacatgg ctctgctgta gtgagtgggt
    12301 tgcgctccgg cagcggtcct gatcaatcgt caccctttct cggtccttca acgttcctga
    12361 caacgagcct ccttttcgcc aatccatcga caatcaccgc gagtccctgc tcgaacgctg
    12421 cgtccggacc ggcttcgtcg aaggcgtcta tcgcggcccg caacagcggc gagagcggag
    12481 cctgttcaac ggtgccgccg cgctcgccgg catcgctgtc gccggcctgc tcctcaagca
    12541 cggccccaac agtgaagtag ctgattgtca tcagcgcatt gacggcgtcc ccggccgaaa
    12601 aacccgcctc gcagaggaag cgaagctgcg cgtcggccgt ttccatctgc ggtgcgcccg
    12661 gtcgcgtgcc ggcatggatg cgcgcgccat cgcggtaggc gagcagcgcc tgcctgaagc
    12721 tgcgggcatt cccgatcaga aatgagcgcc agtcgtcgtc ggctctcggc accgaatgcg
    12781 tatgattctc cgccagcatg gcttcggcca gtgcgtcgag cagcgcccgc ttgttcctga
    12841 agtgccagta aagcgccggc tgctgaaccc ccaaccgttc cgccagtttg cgtgtcgtca
    12901 gaccgtctac gccgacctcg ttcaacaggt ccagggcggc acggatcact gtattcggct
    12961 gcaactttgt catgcttgac actttatcac tgataaacat aatatgtcca ccaacttatc
    13021 agtgataaag aatccgcgcg ttcaatcgga ccagcggagg ctggtccgga ggccagacgt
    13081 gaaacccaac atacccctga tcgtaattct gagcactgtc gcgctcgacg ctgtcggcat
    13141 cggcctgatt atgccggtgc tgccgggcct cctgcgcgat ctggttcact cgaacgacgt
    13201 caccgcccac tatggcattc tgctggcgct gtatgcgttg gtgcaatttg cctgcgcacc
    13261 tgtgctgggc gcgctgtcgg atcgtttcgg gcggcggcca atcttgctcg tctcgctggc
    13321 cggcgccaga tctggggaac cctgtggttg gcatgcacat acaaatggac gaacggataa
    13381 accttttcac gcccttttaa atatccgatt attctaataa acgctctttt ctcttaggtt
    13441 tacccgccaa tatatcctgt caaacactga tagtttaaac tgaaggcggg aaacgacaat
    13501 ctgatcatga gcggagaatt aagggagtca cgttatgacc cccgccgatg acgcgggaca
    13561 agccgtttta cgtttggaac tgacagaacc gcaacgttga aggagccact cagccgcggg
    13621 tttctggagt ttaatgagct aagcacatac gtcagaaacc attattgcgc gttcaaaagt
    13681 cgcctaaggt cactatcagc tagcaaatat ttcttgtcaa aaatgctcca ctgacgttcc
    13741 ataaattccc ctcggtatcc aattagagtc tcatattcac tctcaatcca aataatctgc
    13801 accggatctg gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc
    13861 gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat
    13921 gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg
    13981 tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct ggccacgacg
    14041 ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta
    14101 ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta
    14161 tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc
    14221 gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc
    14281 gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg
    14341 ctcaaggcgc gcatgcccga cggcgatgat ctcgtcgtga cccatggcga tgcctgcttg
    14401 ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt
    14461 gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc
    14521 ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc
    14581 atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgaaatga
    14641 ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg
    14701 aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg
    14761 atctcatgct ggagttcttc gcccacggga tctctgcgga acaggcggtc gaaggtgccg
    14821 atatcattac gacagcaacg gccgacaagc acaacgccac gatcctgagc gacaatatga
    14881 tcgggcccgg cgtccacatc aacggcgtcg gcggcgactg cccaggcaag accgagatgc
    14941 accgcgatat cttgctgcgt tcggatattt tcgtggagtt cccgccacag acccggatga
    15001 tccccgatcg ttcaaacatt tggcaataaa gtttcttaag attgaatcct gttgccggtc
    15061 ttgcgatgat tatcatataa tttctgttga attacgttaa gcatgtaata attaacatgt
    15121 aatgcatgac gttatttatg agatgggttt ttatgattag agtcccgcaa ttatacattt
    15181 aatacgcgat agaaaacaaa atatagcgcg caaactagga taaattatcg cgcgcggtgt
    15241 catctatgtt actagatcgg gcctcctgtc aatgctggcg gcggctctgg tggtggttct
    15301 ggtggcggct ctgagggtgg tggctctgag ggtggcggtt ctgagggtgg cggctctgag
    15361 ggaggcggtt ccggtggtgg ctctggttcc ggtgattttg attatgaaaa gatggcaaac
    15421 gctaataagg gggctatgac cgaaaatgcc gatgaaaacg cgctacagtc tgacgctaaa
    15481 ggcaaacttg attctgtcgc tactgattac ggtgctgcta tcgatggttt cattggtgac
    15541 gtttccggcc ttgctaatgg taatggtgct actggtgatt ttgctggctc taattcccaa
    15601 atggctcaag tcggtgacgg tgataattca cctttaatga ataatttccg tcaatattta
    15661 ccttccctcc ctcaatcggt tgaatgtcgc ccttttgtct ttggcccaat acgcaaaccg
    15721 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
    15781 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag
    15841 gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt
    15901 cacacaggaa acagctatga ccatgattac gccaagcttc ctaggatccc cgatctagta
    15961 acatagatga caccgcgcgc gataatttat cctagtttgc gcgctatatt ttgttttcta
    16021 tcgcgtatta aatgtataat tgcgggactc taatcataaa aacccatctc ataaataacg
    16081 tcatgcatta catgttaatt attacatgct taacgtaatt caacagaaat tatatgataa
    16141 tcatcgcaag accggcaaca ggattcaatc ttaagaaact ttattgccaa atgtttgaac
    16201 gatcgggaat tggatccggt accgagctcg gatccactag taacggccgc cagtgtgctg
    16261 gaattcgccc ttggatccac cactttgtac aagaaagctg ggtcggcgcg cccacccttt
    16321 caaacctttc tcttcttctt aggcactgct tgcttcaaaa ccttatcaaa gtgaccatcc
    16381 atcctggctc ttatagcctc tgctaactgt tcataccttg atctcaatgg tgaacctggc
    16441 ctgtagacca atccaatggt ccttcttggc tctggtttaa tgcatggaag ataaactaca
    16501 ccatccctct tcctctctgg tggcacggca agtgctggta acaaagttat tccacttcca
    16561 acggctacca tgttcctcaa ggtttctagt gatgtagccc tgaagtgagt atcctcgtca
    16621 gcaccggctt cgaagcaaaa tccggttccg ttgtactcta gcttgtgtcc aagaatattt
    16681 ccgtcctctt tgaatccgat acctttaagc tctattctat tcaccaaagt atctccctca
    16741 aacttcactt ctgccctagt tttataatta ccgtcgtctt tgaagaatat agtcctctct
    16801 tgcacatatc cctcaggcat agcactcttg aagaagtcat gttgcttcat atggtcagga
    16861 tatcttgcga agcactttaa accgtaacct aaggttgtta ctaatgttgg ccatgggaca
    16921 ggaagttttc cagttgtaca gattaatttc aatgttagtt ttccgtaggt tgcgtcaccc
    16981 tcaccttctc cggagactga aaatttgtga ccgttcacgt caccatcaag ttctactaat
    17041 attgggacaa ctcctgtgaa caactcttct cccttggaac ctgtacctcc actacctccg
    17101 tctacattgt atagctcatc cataccaaga gtaataccgg cagcggttac gaactcaagc
    17161 aacaccatgt gatccctttt ctcatttggg tctttggata ggacagactg aaaagatagg
    17221 tagtggttat ctggaagtaa aacaggtccg tcaccgattg gggtgttctg ttgataatgg
    17281 tctgctagtt gtacggaacc atcttccacg ttatgtctaa ttttgaagtt ggcctttata
    17341 ccattcttct gtttgtcggc catgatatag acgttatctg aattgtaacc ggcggacata
    17401 gcttgatccc tcaagcagtg tccatcctca agcattagaa gtttctcacc ggccaaatct
    17461 gccattggaa cgcattctct attggcccat gggtgatctt catagattgc taaaagcata
    17521 ggttcatcga aaagaggcac ttctatgaat gcctcggatt cctttaccaa agctagtatg
    17581 acacaatcaa gtttaccact atctaactgg gccaatagtt gatgagtctg tgcctcgtga
    17641 aggtacattt caagtttagg gaaagtttgg tgtagcattg ggataatgtg aggaagtaag
    17701 taaggtccaa ctgttgggat tagaccgata tgcaatggtc ctgacatagt ctcaccctgt
    17761 tgagaagcca tttcaacctt tctcttcttc ttaggcatgg tgaagggggc ggccgcggag
    17821 cctgcttttt tgtacaaact tgttctagaa ggattggttg agtatctgat gatccttcaa
    17881 atgggaatga atgccttctt atatagaggg aattcttttg tggtcgtcac tgcgttcgtc
    17941 atacgcatta gtgagtgggc tgtcaggaca gctcttttcc acgttatttt gttccccact
    18001 tgtactagag gaatctgctt tatctttgca ataaaggcaa agatgctttt ggtaggtgcg
    18061 cctaacaatt ctgcaccatt ccttttttgt ctggtcccca caagccagct gctcgatgtt
    18121 gacaagatta ctttcaaaga tgcccactaa ctttaagtct tcggtggatg tctttttctg
    18181 aaacttactg accatgatgc atgtgctgga acagtagttt actttgattg aagattcttc
    18241 attgatctcc tgtagctttt ggctaatggt ttggagactc tgtaccctga ccttgttgag
    18301 gctttggact gagaattctt ccttacaaac ctttgaggat gggagttcct tcttggtttt
    18361 ggcgatacca atttgaataa agtgatatgg ctcgtacctt gttgattgaa cccaatctgg
    18421 aatgctgcta aatcctgagc tcaagcttgc atgcctgcag gtccccagat tagccttttc
    18481 aatttcagaa agaatgctaa cccacagatg gttagagagg cttacgcagc aggtctcatc
    18541 aagacgatct acccgagcaa taatctccag gaaatcaaat accttcccaa gaaggttaaa
    18601 gatgcagtca aaagattcag gactaactgc atcaagaaca cagagaaaga tatatttctc
    18661 aagatcagaa gtactattcc agtatggacg attcaaggct tgcttcacaa accaaggcaa
    18721 gtaatagaga ttggagtctc taaaaaggta gttcccactg aatcaaaggc catggagtca
    18781 aagattcaaa tagaggacct aacagaactc gccgtaaaga ctggcgaaca gttcatacag
    18841 agtctcttac gactcaatga caagaagaaa atcttcgtca acatggtgga gcacgacaca
    18901 cttgtctact ccaaaaatat caaagataca gtctcagaag accaaagggc aattgagact
    18961 tttcaacaaa gggtaatatc cggaaacctc ctcggattcc attgcccagc tatctgtcac
    19021 tttattgtga agatagtgga aaaggaaggt ggctcctaca aatgccatca ttgcgataaa
    19081 ggaaaggcca tcgttgaaga tgcctctgcc gacagtggtc ccaaagatgg acccccaccc
    19141 acgaggagca tcgtggaaaa agaagacgtt ccaaccacgt cttcaaagca agtggattga
    19201 tgtgatatct ccactgacgt aagggatgac gcacaatccc actatccttc gcaagaccct
    19261 tcctc
//
