LOCUS       sequence-180317-       19466 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Simultaneous expresion of stromal sHyPer and stromal Ascorbate 
            peroxidase (APX) in plant cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 19466)
  AUTHORS   Exposito-Rodriguez M, Laissue PP, Yvon-Durocher G, Smirnoff N, 
            Mullineaux PM
  TITLE     Photosynthesis-dependent H2O2 transfer from chloroplasts to nuclei 
            provides a high-light signalling mechanism.
  JOURNAL   Nat Commun. 2017 Jun 29;8(1):49. doi: 10.1038/s41467-017-00074-w.
  PUBMED    28663550
REFERENCE   2  (bases 1 to 19466)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2017-06-29"; volume: "8(1):49. doi"; pages: " 
            10.1038/s41467-017-00074-w"
COMMENT     Sequence Label: pGem-sHyper:sAPX
FEATURES             Location/Qualifiers
     source          1..19466
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(430..454)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        1565..1910
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    1932..1956
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3673..3697)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      3729..3981
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      complement(4410..4662)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(5130..6155)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(5130..6155)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(6165..6509)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(6651..6668)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6651..6667)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6660..6682)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(6807..6826)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7017..7038
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    7285..7309
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(8905..10053)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(10352..11146)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  11338..12105
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      12728..13439
                     /label=oriV
                     /note="incP origin of replication"
     CDS             complement(13566..13937)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            13970..14079
                     /note="incP origin of transfer"
     primer_bind     14302..14321
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     14555..14572
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(14760..15410)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    15875..15899
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        16023..16202
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        16055..16238
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             16259..17053
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(16313..16332)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     16923..16942
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      17443..17695
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    18244..18265
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(18280..18285,18286..18303,18304..18310)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    18318..18334
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     18323..18345
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     18342..18358
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     18342..18358
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     terminator      complement(18389..18641)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    18716..18740
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 taccagcatc atgccaacct aatctaacca aaatcggatg gcagaatttg gtgctgagaa
       61 gctctttgat atcttctcta gcgttcttca actgatcagg atctgaagat gaagatttag
      121 tagcggcggt ggtggagttg aaactccgat tcaccgacgc aattcgatat ttcttctgag
      181 agagggatga agaagaagag agacgtggag aagagacgag agatctgacg aaggagagag
      241 aggaggaagc ggagagagtg agagtggatc tggaggagga ggaggaggag cggaggagaa
      301 gagaagcggc ggtggtagat cggagagaag aagacattgt tgttgttgtt gttgtgggag
      361 gaggagaaag gagggttccg ttgagtgtga gagacacacg ctctgccatg gtgaaggggg
      421 cggccgcgga gcctgctttt ttgtacaaac ttgttctaga aggattggtt gagtatctga
      481 tgatccttca aatgggaatg aatgccttct tatatagagg gaattctttt gtggtcgtca
      541 ctgcgttcgt catacgcatt agtgagtggg ctgtcaggac agctcttttc cacgttattt
      601 tgttccccac ttgtactaga ggaatctgct ttatctttgc aataaaggca aagatgcttt
      661 tggtaggtgc gcctaacaat tctgcaccat tccttttttg tctggtcccc acaagccagc
      721 tgctcgatgt tgacaagatt actttcaaag atgcccacta actttaagtc ttcggtggat
      781 gtctttttct gaaacttact gaccatgatg catgtgctgg aacagtagtt tactttgatt
      841 gaagattctt cattgatctc ctgtagcttt tggctaatgg tttggagact ctgtaccctg
      901 accttgttga ggctttggac tgagaattct tccttacaaa cctttgagga tgggagttcc
      961 ttcttggttt tggcgatacc aatttgaata aagtgatatg gctcgtacct tgttgattga
     1021 acccaatctg gaatgctgct aaatcctgag ctcaagcttg catgcctgca ggtccccaga
     1081 ttagcctttt caatttcaga aagaatgcta acccacagat ggttagagag gcttacgcag
     1141 caggtctcat caagacgatc tacccgagca ataatctcca ggaaatcaaa taccttccca
     1201 agaaggttaa agatgcagtc aaaagattca ggactaactg catcaagaac acagagaaag
     1261 atatatttct caagatcaga agtactattc cagtatggac gattcaaggc ttgcttcaca
     1321 aaccaaggca agtaatagag attggagtct ctaaaaaggt agttcccact gaatcaaagg
     1381 ccatggagtc aaagattcaa atagaggacc taacagaact cgccgtaaag actggcgaac
     1441 agttcataca gagtctctta cgactcaatg acaagaagaa aatcttcgtc aacatggtgg
     1501 agcacgacac acttgtctac tccaaaaata tcaaagatac agtctcagaa gaccaaaggg
     1561 caattgagac ttttcaacaa agggtaatat ccggaaacct cctcggattc cattgcccag
     1621 ctatctgtca ctttattgtg aagatagtgg aaaaggaagg tggctcctac aaatgccatc
     1681 attgcgataa aggaaaggcc atcgttgaag atgcctctgc cgacagtggt cccaaagatg
     1741 gacccccacc cacgaggagc atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc
     1801 aagtggattg atgtgatatc tccactgacg taagggatga cgcacaatcc cactatcctt
     1861 cgcaagaccc ttcctctata taaggaagtt catttcattt ggagagaaca cgggggactc
     1921 tagagttatc aacaagtttg tacaaaaaag caggctccgc ggccgccccc ttcaccatgg
     1981 cttcctctat gctctcttcc gctactatgg ttgcctctcc ggctcaggcc actatggtcg
     2041 ctcctttcaa cggacttaag tcctccgctg ccttcccagc cacccgcaag gctaacaacg
     2101 acattacttc catcacaagc aacggcggaa gagttaactg catgcaggtg tggcctccga
     2161 ttggaaagaa gaagtttgag actctctctt accttcctga ccttaccgat tccgaaaagc
     2221 ttgaaatggc ttctcaacag ggtgagacta tgtcaggacc attgcatatc ggtctaatcc
     2281 caacagttgg accttactta cttcctcaca ttatcccaat gctacaccaa actttcccta
     2341 aacttgaaat gtaccttcac gaggcacaga ctcatcaact attggcccag ttagatagtg
     2401 gtaaacttga ttgtgtcata ctagctttgg taaaggaatc cgaggcattc atagaagtgc
     2461 ctcttttcga tgaacctatg cttttagcaa tctatgaaga tcacccatgg gccaatagag
     2521 aatgcgttcc aatggcagat ttggccggtg agaaacttct aatgcttgag gatggacact
     2581 gcttgaggga tcaagctatg tccgccggtt acaattcaga taacgtctat atcatggccg
     2641 acaaacagaa gaatggtata aaggccaact tcaaaattag acataacgtg gaagatggtt
     2701 ccgtacaact agcagaccat tatcaacaga acaccccaat cggtgacgga cctgttttac
     2761 ttccagataa ccactaccta tcttttcagt ctgtcctatc caaagaccca aatgagaaaa
     2821 gggatcacat ggtgttgctt gagttcgtaa ccgctgccgg tattactctt ggtatggatg
     2881 agctatacaa tgtagacgga ggtagtggag gtacaggttc caagggagaa gagttgttca
     2941 caggagttgt cccaatatta gtagaacttg atggtgacgt gaacggtcac aaattttcag
     3001 tctccggaga aggtgagggt gacgcaacct acggaaaact aacattgaaa ttaatctgta
     3061 caactggaaa acttcctgtc ccatggccaa cattagtaac aaccttaggt tacggtttaa
     3121 agtgcttcgc aagatatcct gaccatatga agcaacatga cttcttcaag agtgctatgc
     3181 ctgagggata tgtgcaagag aggactatat tcttcaaaga cgacggtaat tataaaacta
     3241 gggcagaagt gaagtttgag ggagatactt tggtgaatag aatagagctt aaaggtatcg
     3301 gattcaaaga ggacggaaat attcttggac acaagctaga gtacaacgga accggatttt
     3361 gcttcgaagc cggtgctgac gaggatactc acttcagggc tacatcacta gaaaccttga
     3421 ggaacatggt agccgcagga agtggaataa ctttgttacc agcacttgcc gtgccaccag
     3481 agaggaagag ggatggtgta gtttatcttc catgcattaa accagagcca agaaggacca
     3541 ttggattggt ctacaggcca ggttcaccat tgagatcaag gtatgaacag ttagcagagg
     3601 ctataagagc caggatggat ggtcactttg ataaggtttt gaagcaagca gtgtgaaagg
     3661 gtgggcgcgc cgacccagct ttcttgtaca aagtggttga taacagcgct tagagctcga
     3721 atttccccga tcgttcaaac atttggcaat aaagtttctt aagattgaat cctgttgccg
     3781 gtcttgcgat gattatcata taatttctgt tgaattacgt taagcatgta ataattaaca
     3841 tgtaatgcat gacgttattt atgagatggg tttttatgat tagagtcccg caattataca
     3901 tttaatacgc gatagaaaac aaaatatagc gcgcaaacta ggataaatta tcgcgcgcgg
     3961 tgtcatctat gttactagat cgggaattag cttcatcaac gcaagacatg cgcacgaccg
     4021 tctgacagga gaggaatttc cgacgagcac agaaaggact tgctcttgga cgtaggccta
     4081 tttctcaggc acatgtatca agtgttcgga cgtgggtttt cgatggtgta tcagccgccg
     4141 ccaactggga gatgaggagg ctttcttggg gggcagtcag cagttcattt cacaagacag
     4201 aggaacttgt aaggagatgc actgatttat cttggcgcaa accagcagga cgaattagtg
     4261 ggaatagccc gcgaatatct aagttatgcc tgtcggcatg agcagaaact tccaattcga
     4321 aacagtttgg agaggttgtt tttgggcata ccttttgtta gtcagcctct cgattgctca
     4381 tcgtcattac acagtaccga agtttgatcg atctagtaac atagatgaca ccgcgcgcga
     4441 taatttatcc tagtttgcgc gctatatttt gttttctatc gcgtattaaa tgtataattg
     4501 cgggactcta atcataaaaa cccatctcat aaataacgtc atgcattaca tgttaattat
     4561 tacatgctta acgtaattca acagaaatta tatgataatc atcgcaagac cggcaacagg
     4621 attcaatctt aagaaacttt attgccaaat gtttgaacga tctgcttcga cgcactcctt
     4681 ctttactcca ccatctcgtc cttattgaaa acgtgggtag caccaaaacg aatcaagtcg
     4741 ctggaactga agttaccaat cacgctggat gatttgccag ttggattaat cttgcctttc
     4801 cccgcatgaa taatattgat gaatgcatgc gtgaggggta tttcgatttt ggcaatagct
     4861 gcaattgccg cgacatcctc caacgagcat aattcttcag aaaaatagcg atgttccatg
     4921 ttgtcagggc atgcatgatg cacgttatga ggtgacggtg ctaggcagta ttccctcaaa
     4981 gtttcatagt cagtatcata ttcatcattg cattcctgca agagagaatt gagacgcaat
     5041 ccacacgctg cggcaacctt ccggcgttcg tggtctattt gctcttggac gttgcaaacg
     5101 taagtgttgg atcccggtcg gcatctactc tattcctttg ccctcggacg agtgctgggg
     5161 cgtcggtttc cactatcggc gagtacttct acacagccat cggtccagac ggccgcgctt
     5221 ctgcgggcga tttgtgtacg cccgacagtc ccggctccgg atcggacgat tgcgtcgcat
     5281 cgaccctgcg cccaagctgc atcatcgaaa ttgccgtcaa ccaagctctg atagagttgg
     5341 tcaagaccaa tgcggagcat atacgcccgg agccgcggcg atcctgcaag ctccggatgc
     5401 ctccgctcga agtagcgcgt ctgctgctcc atacaagcca accacggcct ccagaagaag
     5461 atgttggcga cctcgtattg ggaatccccg aacatcgcct cgctccagtc aatgaccgct
     5521 gttatgcggc cattgtccgt caggacattg ttggagccga aatccgcgtg cacgaggtgc
     5581 cggacttcgg ggcagtcctc ggcccaaagc atcagctcat cgagagcctg cgcgacggac
     5641 gcactgacgg tgtcgtccat cacagtttgc cagtgataca catggggatc agcaatcgcg
     5701 catatgaaat cacgccatgt agtgtattga ccgattcctt gcggtccgaa tgggccgaac
     5761 ccgctcgtct ggctaagatc ggccgcagcg atcgcatcca tggcctccgc gaccggctgc
     5821 agaacagcgg gcagttcggt ttcaggcagg tcttgcaacg tgacaccctg tgcacggcgg
     5881 gagatgcaat aggtcaggct ctcgctgaat tccccaatgt caagcacttc cggaatcggg
     5941 agcgcggccg atgcaaagtg ccgataaaca taacgatctt tgtagaaacc atcggcgcag
     6001 ctatttaccc gcaggacata tccacgccct cctacatcga agctgaaagc acgagattct
     6061 tcgccctccg agagctgcat caggtcggag acgctgtcga acttttcgat cagaaacttc
     6121 tcgacagacg tcgcggtgag ttcaggcttt ttcatatcgg ggtcgtcctc tccaaatgaa
     6181 atgaacttcc ttatatagag gaagggtctt gcgaaggata gtgggattgt gcgtcatccc
     6241 ttacgtcagt ggagatatca catcaatcca cttgctttga agacgtggtt ggaacgtctt
     6301 ctttttccac gatgctcctc gtgggtgggg gtccatcttt gggaccactg tcggcagagg
     6361 catcttgaac gatagccttt cctttatcgc aatgatggca tttgtaggtg ccaccttcct
     6421 tttctactgt ccttttgatg aagtgacaga tagctgggca atggaatccg aggaggtttc
     6481 ccgatattac cctttgttga aaagtctcaa tagccctttg gtcttctgag actgtatctt
     6541 tgatattctt ggagtagacg agagtgtcgt gctccaccat gttgacggat ctctaggacg
     6601 cgtcgacgcg tcgacgcgtc gacgcgtcga cgcgtcctag aagctaattc actggccgtc
     6661 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
     6721 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
     6781 cagttgcgca gcctgaatgg cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     6841 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     6901 tgctttacgg cacctcgacc ccaaaaaact tgatttgggt gatggttcac gtagtgggcc
     6961 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     7021 actcttgttc caaactggaa caacactcaa ccctatctcg ggctattctt ttgatttata
     7081 agggattttg ccgatttcgg aaccaccatc aaacaggatt ttcgcctgct ggggcaaacc
     7141 agcgtggacc gcttgctgca actctctcag ggccaggcgg tgaagggcaa tcagctgttg
     7201 cccgtctcac tggtgaaaag aaaaaccacc ccagtacatt aaaaacgtcc gcaatgtgtt
     7261 attaagttgt ctaagcgtca atttgtttac accacaatat atcctgccac cagccagcca
     7321 acagctcccc gaccggcagc tcggcacaaa atcaccactc gatacaggca gcccatcagt
     7381 ccgggacggc gtcagcggga gagccgttgt aaggcggcag actttgctca tgttaccgat
     7441 gctattcgga agaacggcaa ctaagctgcc gggtttgaaa cacggatgat ctcgcggagg
     7501 gtagcatgtt gattgtaacg atgacagagc gttgctgcct gtgatcaaat atcatctccc
     7561 tcgcagagat ccgaattatc agccttctta ttcatttctc gcttaaccgt gacaggctgt
     7621 cgatcttgag aactatgccg acataatagg aaatcgctgg ataaagccgc tgaggaagct
     7681 gagtggcgct atttctttag aagtgaacgt tgacgatatc aactccccta tccattgctc
     7741 accgaatggt acaggtcggg gacccgaagt tccgactgtc ggcctgatgc atccccggct
     7801 gatcgacccc agatctgggg ctgagaaagc ccagtaagga aacaactgta ggttcgagtc
     7861 gcgagatccc ccggaaccaa aggaagtagg ttaaacccgc tccgatcagg ccgagccacg
     7921 ccaggccgag aacattggtt cctgtaggca tcgggattgg cggatcaaac actaaagcta
     7981 ctggaacgag cagaagtcct ccggccgcca gttgccaggc ggtaaaggtg agcagaggca
     8041 cgggaggttg ccacttgcgg gtcagcacgg ttccgaacgc catggaaacc gcccccgcca
     8101 ggcccgctgc gacgccgaca ggatctagcg ctgcgtttgg tgtcaacacc aacagcgcca
     8161 cgcccgcagt tccgcaaata gcccccagga ccgccatcaa tcgtatcggg ctacctagca
     8221 gagcggcaga gatgaacacg accatcagcg gctgcacagc gcctaccgtc gccgcgaccc
     8281 cgcccggcag gcggtagacc gaaataaaca acaagctcca gaatagcgaa atattaagtg
     8341 cgccgaggat gaagatgcgc atccaccaga ttcccgttgg aatctgtcgg acgatcatca
     8401 cgagcaataa acccgccggc aacgcccgca gcagcatacc ggcgacccct cggcctcgct
     8461 gttcgggctc cacgaaaacg ccggacagat gcgccttgtg agcgtccttg gggccgtcct
     8521 cctgtttgaa gaccgacagc ccaatgatct cgccgtcgat gtaggcgccg aatgccacgg
     8581 catctcgcaa ccgttcagcg aacgcctcca tgggcttttt ctcctcgtgc tcgtaaacgg
     8641 acccgaacat ctctggagct ttcttcaggg ccgacaatcg gatctcgcgg aaatcctgca
     8701 cgtcggccgc tccaagccgt cgaatctgag ccttaatcac aattgtcaat tttaatcctc
     8761 tgtttatcgg cagttcgtag agcgcgccgt gcgtcccgag cgatactgag cgaagcaagt
     8821 gcgtcgagca gtgcccgctt gttcctgaaa tgccagtaaa gcgctggctg ctgaaccccc
     8881 agccggaact gaccccacaa ggccctagcg tttgcaatgc accaggtcat cattgaccca
     8941 ggcgtgttcc accaggccgc tgcctcgcaa ctcttcgcag gcttcgccga cctgctcgcg
     9001 ccacttcttc acgcgggtgg aatccgatcc gcacatgagg cggaaggttt ccagcttgag
     9061 cgggtacggc tcccggtgcg agctgaaata gtcgaacatc cgtcgggccg tcggcgacag
     9121 cttgcggtac ttctcccata tgaatttcgt gtagtggtcg ccagcaaaca gcacgacgat
     9181 ttcctcgtcg atcaggacct ggcaacggga cgttttcttg ccacggtcca ggacgcggaa
     9241 gcggtgcagc agcgacaccg attccaggtg cccaacgcgg tcggacgtga agcccatcgc
     9301 cgtcgcctgt aggcgcgaca ggcattcctc ggccttcgtg taataccggc cattgatcga
     9361 ccagcccagg tcctggcaaa gctcgtagaa cgtgaaggtg atcggctcgc cgataggggt
     9421 gcgcttcgcg tactccaaca cctgctgcca caccagttcg tcatcgtcgg cccgcagctc
     9481 gacgccggtg taggtgatct tcacgtcctt gttgacgtgg aaaatgacct tgttttgcag
     9541 cgcctcgcgc gggattttct tgttgcgcgt ggtgaacagg gcagagcggg ccgtgtcgtt
     9601 tggcatcgct cgcatcgtgt ccggccacgg cgcaatatcg aacaaggaaa gctgcatttc
     9661 cttgatctgc tgcttcgtgt gtttcagcaa cgcggcctgc ttggcctcgc tgacctgttt
     9721 tgccaggtcc tcgccggcgg tttttcgctt cttggtcgtc atagttcctc gcgtgtcgat
     9781 ggtcatcgac ttcgccaaac ctgccgcctc ctgttcgaga cgacgcgaac gctccacggc
     9841 ggccgatggc gcgggcaggg cagggggagc cagttgcacg ctgtcgcgct cgatcttggc
     9901 cgtagcttgc tggaccatcg agccgacgga ctggaaggtt tcgcggggcg cacgcatgac
     9961 ggtgcggctt gcgatggttt cggcatcctc ggcggaaaac cccgcgtcga tcagttcttg
    10021 cctgtatgcc ttccggtcaa acgtccgatt cattcaccct ccttgcggga ttgccccgac
    10081 tcacgccggg gcaatgtgcc cttattcctg atttgacccg cctggtgcct tggtgtccag
    10141 ataatccacc ttatcggcaa tgaagtcggt cccgtagacc gtctggccgt ccttctcgta
    10201 cttggtattc cgaatcttgc cctgcacgaa taccagcgac cccttgccca aatacttgcc
    10261 gtgggcctcg gcctgagagc caaaacactt gatgcggaag aagtcggtgc gctcctgctt
    10321 gtcgccggca tcgttgcgcc acatctaggt actaaaacaa ttcatccagt aaaatataat
    10381 attttatttt ctcccaatca ggcttgatcc ccagtaagtc aaaaaatagc tcgacatact
    10441 gttcttcccc gatatcctcc ctgatcgacc ggacgcagaa ggcaatgtca taccacttgt
    10501 ccgccctgcc gcttctccca agatcaataa agccacttac tttgccatct ttcacaaaga
    10561 tgttgctgtc tcccaggtcg ccgtgggaaa agacaagttc ctcttcgggc ttttccgtct
    10621 ttaaaaaatc atacagctcg cgcggatctt taaatggagt gtcttcttcc cagttttcgc
    10681 aatccacatc ggccagatcg ttattcagta agtaatccaa ttcggctaag cggctgtcta
    10741 agctattcgt atagggacaa tccgatatgt cgatggagtg aaagagcctg atgcactccg
    10801 catacagctc gataatcttt tcagggcttt gttcatcttc atactcttcc gagcaaagga
    10861 cgccatcggc ctcactcatg agcagattgc tccagccatc atgccgttca aagtgcagga
    10921 cctttggaac aggcagcttt ccttccagcc atagcatcat gtccttttcc cgttccacat
    10981 cataggtggt ccctttatac cggctgtccg tcatttttaa atataggttt tcattttctc
    11041 ccaccagctt atatacctta gcaggagaca ttccttccgt atcttttacg cagcggtatt
    11101 tttcgatcag ttttttcaat tccggtgata ttctcatttt agccatttat tatttccttc
    11161 ctcttttcta cagtatttaa agatacccca agaagctaat tataacaaga cgaactccaa
    11221 ttcactgttc cttgcattct aaaaccttaa ataccagaaa acagcttttt caaagttgtt
    11281 ttcaaagttg gcgtataaca tagtatcgac ggagccgatt ttgaaaccac aattatgggt
    11341 gatgctgcca acttactgat ttagtgtatg atggtgtttt tgaggtgctc cagtggcttc
    11401 tgtttctatc agctgtccct cctgttcagc tactgacggg gtggtgcgta acggcaaaag
    11461 caccgccgga catcagcgct atctctgctc tcactgccgt aaaacatggc aactgcagtt
    11521 cacttacacc gcttctcaac ccggtacgca ccagaaaatc attgatatgg ccatgaatgg
    11581 cgttggatgc cgggcaacag cccgcattat gggcgttggc ctcaacacga ttttacgtca
    11641 cttaaaaaac tcaggccgca gtcggtaacc tcgcgcatac agccgggcag tgacgtcatc
    11701 gtctgcgcgg aaatggacga acagtggggc tatgtcgggg ctaaatcgcg ccagcgctgg
    11761 ctgttttacg cgtatgacag tctccggaag acggttgttg cgcacgtatt cggtgaacgc
    11821 actatggcga cgctggggcg tcttatgagc ctgctgtcac cctttgacgt ggtgatatgg
    11881 atgacggatg gctggccgct gtatgaatcc cgcctgaagg gaaagctgca cgtaatcagc
    11941 aagcgatata cgcagcgaat tgagcggcat aacctgaatc tgaggcagca cctggcacgg
    12001 ctgggacgga agtcgctgtc gttctcaaaa tcggtggagc tgcatgacaa agtcatcggg
    12061 cattatctga acataaaaca ctatcaataa gttggagtca ttacccaatt atgatagaat
    12121 ttacaagcta taaggttatt gtcctgggtt tcaagcatta gtccatgcaa gtttttatgc
    12181 tttgcccatt ctatagatat attgataagc gcgctgccta tgccttgccc cctgaaatcc
    12241 ttacatacgg cgatatcttc tatataaaag atatattatc ttatcagtat tgtcaatata
    12301 ttcaaggcaa tctgcctcct catcctcttc atcctcttcg tcttggtagc tttttaaata
    12361 tggcgcttca tagagtaatt ctgtaaaggt ccaattctcg ttttcatacc tcggtataat
    12421 cttacctatc acctcaaatg gttcgctggg tttatcgcac ccccgaacac gagcacggca
    12481 cccgcgacca ctatgccaag aatgcccaag gtaaaaattg ccggccccgc catgaagtcc
    12541 gtgaatgccc cgacggccga agtgaagggc aggccgccac ccaggccgcc gccctcactg
    12601 cccggcacct ggtcgctgaa tgtcgatgcc agcacctgcg gcacgtcaat gcttccgggc
    12661 gtcgcgctcg ggctgatcgc ccatcccgtt actgccccga tcccggcaat ggcaaggact
    12721 gccagcgctg ccatttttgg ggtgaggccg ttcgcggccg aggggcgcag cccctggggg
    12781 gatgggaggc ccgcgttagc gggccgggag ggttcgagaa gggggggcac cccccttcgg
    12841 cgtgcgcggt cacgcgcaca gggcgcagcc ctggttaaaa acaaggttta taaatattgg
    12901 tttaaaagca ggttaaaaga caggttagcg gtggccgaaa aacgggcgga aacccttgca
    12961 aatgctggat tttctgcctg tggacagccc ctcaaatgtc aataggtgcg cccctcatct
    13021 gtcagcactc tgcccctcaa gtgtcaagga tcgcgcccct catctgtcag tagtcgcgcc
    13081 cctcaagtgt caataccgca gggcacttat ccccaggctt gtccacatca tctgtgggaa
    13141 actcgcgtaa aatcaggcgt tttcgccgat ttgcgaggct ggccagctcc acgtcgccgg
    13201 ccgaaatcga gcctgcccct catctgtcaa cgccgcgccg ggtgagtcgg cccctcaagt
    13261 gtcaacgtcc gcccctcatc tgtcagtgag ggccaagttt tccgcgaggt atccacaacg
    13321 ccggcggccg cggtgtctcg cacacggctt cgacggcgtt tctggcgcgt ttgcagggcc
    13381 atagacggcc gccagcccag cggcgagggc aaccagcccg gtgagcgtcg gaaaggcgct
    13441 cggtcttgcc ttgctcgtcg gtgatgtact tcaccagctc cgcgaagtcg ctcttcttga
    13501 tggagcgcat ggggacgtgc ttggcaatca cgcgcacccc ccggccgttt tagcggctaa
    13561 aaaagtcatg gctctgccct cgggcggacc acgcccatca tgaccttgcc aagctcgtcc
    13621 tgcttctctt cgatcttcgc cagcagggcg aggatcgtgg catcaccgaa ccgcgccgtg
    13681 cgcgggtcgt cggtgagcca gagtttcagc aggccgccca ggcggcccag gtcgccattg
    13741 atgcgggcca gctcgcggac gtgctcatag tccacgacgc ccgtgatttt gtagccctgg
    13801 ccgacggcca gcaggtaggc cgacaggctc atgccggccg ccgccgcctt ttcctcaatc
    13861 gctcttcgtt cgtctggaag gcagtacacc ttgataggtg ggctgccctt cctggttggc
    13921 ttggtttcat cagccatccg cttgccctca tctgttacgc cggcggtagc cggccagcct
    13981 cgcagagcag gattcccgtt gagcaccgcc aggtgcgaat aagggacagt gaagaaggaa
    14041 cacccgctcg cgggtgggcc tacttcacct atcctgcccg gctgacgccg ttggatacac
    14101 caaggaaagt ctacacgaac cctttggcaa aatcctgtat atcgtgcgaa aaaggatgga
    14161 tataccgaaa aaatcgctat aatgaccccg aagcagggtt atgcagcgga aaagcgccac
    14221 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
    14281 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
    14341 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
    14401 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
    14461 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
    14521 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
    14581 agagcgccag aaggccgcca gagaggccga gcgcggccgt gaggcttgga cgctagggca
    14641 gggcatgaaa aagcccgtag cgggctgcta cgggcgtctg acgcggtgga aagggggagg
    14701 ggatgttgtc tacatggctc tgctgtagtg agtgggttgc gctccggcag cggtcctgat
    14761 caatcgtcac cctttctcgg tccttcaacg ttcctgacaa cgagcctcct tttcgccaat
    14821 ccatcgacaa tcaccgcgag tccctgctcg aacgctgcgt ccggaccggc ttcgtcgaag
    14881 gcgtctatcg cggcccgcaa cagcggcgag agcggagcct gttcaacggt gccgccgcgc
    14941 tcgccggcat cgctgtcgcc ggcctgctcc tcaagcacgg ccccaacagt gaagtagctg
    15001 attgtcatca gcgcattgac ggcgtccccg gccgaaaaac ccgcctcgca gaggaagcga
    15061 agctgcgcgt cggccgtttc catctgcggt gcgcccggtc gcgtgccggc atggatgcgc
    15121 gcgccatcgc ggtaggcgag cagcgcctgc ctgaagctgc gggcattccc gatcagaaat
    15181 gagcgccagt cgtcgtcggc tctcggcacc gaatgcgtat gattctccgc cagcatggct
    15241 tcggccagtg cgtcgagcag cgcccgcttg ttcctgaagt gccagtaaag cgccggctgc
    15301 tgaaccccca accgttccgc cagtttgcgt gtcgtcagac cgtctacgcc gacctcgttc
    15361 aacaggtcca gggcggcacg gatcactgta ttcggctgca actttgtcat gcttgacact
    15421 ttatcactga taaacataat atgtccacca acttatcagt gataaagaat ccgcgcgttc
    15481 aatcggacca gcggaggctg gtccggaggc cagacgtgaa acccaacata cccctgatcg
    15541 taattctgag cactgtcgcg ctcgacgctg tcggcatcgg cctgattatg ccggtgctgc
    15601 cgggcctcct gcgcgatctg gttcactcga acgacgtcac cgcccactat ggcattctgc
    15661 tggcgctgta tgcgttggtg caatttgcct gcgcacctgt gctgggcgcg ctgtcggatc
    15721 gtttcgggcg gcggccaatc ttgctcgtct cgctggccgg cgccagatct ggggaaccct
    15781 gtggttggca tgcacataca aatggacgaa cggataaacc ttttcacgcc cttttaaata
    15841 tccgattatt ctaataaacg ctcttttctc ttaggtttac ccgccaatat atcctgtcaa
    15901 acactgatag tttaaactga aggcgggaaa cgacaatctg atcatgagcg gagaattaag
    15961 ggagtcacgt tatgaccccc gccgatgacg cgggacaagc cgttttacgt ttggaactga
    16021 cagaaccgca acgttgaagg agccactcag ccgcgggttt ctggagttta atgagctaag
    16081 cacatacgtc agaaaccatt attgcgcgtt caaaagtcgc ctaaggtcac tatcagctag
    16141 caaatatttc ttgtcaaaaa tgctccactg acgttccata aattcccctc ggtatccaat
    16201 tagagtctca tattcactct caatccaaat aatctgcacc ggatctggat cgtttcgcat
    16261 gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg
    16321 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc
    16381 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca
    16441 ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct
    16501 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga
    16561 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg
    16621 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat
    16681 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga
    16741 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg
    16801 cgatgatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg
    16861 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat
    16921 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct
    16981 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga
    17041 cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg
    17101 ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt
    17161 ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc
    17221 cacgggatct ctgcggaaca ggcggtcgaa ggtgccgata tcattacgac agcaacggcc
    17281 gacaagcaca acgccacgat cctgagcgac aatatgatcg ggcccggcgt ccacatcaac
    17341 ggcgtcggcg gcgactgccc aggcaagacc gagatgcacc gcgatatctt gctgcgttcg
    17401 gatattttcg tggagttccc gccacagacc cggatgatcc ccgatcgttc aaacatttgg
    17461 caataaagtt tcttaagatt gaatcctgtt gccggtcttg cgatgattat catataattt
    17521 ctgttgaatt acgttaagca tgtaataatt aacatgtaat gcatgacgtt atttatgaga
    17581 tgggttttta tgattagagt cccgcaatta tacatttaat acgcgataga aaacaaaata
    17641 tagcgcgcaa actaggataa attatcgcgc gcggtgtcat ctatgttact agatcgggcc
    17701 tcctgtcaat gctggcggcg gctctggtgg tggttctggt ggcggctctg agggtggtgg
    17761 ctctgagggt ggcggttctg agggtggcgg ctctgaggga ggcggttccg gtggtggctc
    17821 tggttccggt gattttgatt atgaaaagat ggcaaacgct aataaggggg ctatgaccga
    17881 aaatgccgat gaaaacgcgc tacagtctga cgctaaaggc aaacttgatt ctgtcgctac
    17941 tgattacggt gctgctatcg atggtttcat tggtgacgtt tccggccttg ctaatggtaa
    18001 tggtgctact ggtgattttg ctggctctaa ttcccaaatg gctcaagtcg gtgacggtga
    18061 taattcacct ttaatgaata atttccgtca atatttacct tccctccctc aatcggttga
    18121 atgtcgccct tttgtctttg gcccaatacg caaaccgcct ctccccgcgc gttggccgat
    18181 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc
    18241 aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc
    18301 tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca
    18361 tgattacgcc aagcttccta ggatccccga tctagtaaca tagatgacac cgcgcgcgat
    18421 aatttatcct agtttgcgcg ctatattttg ttttctatcg cgtattaaat gtataattgc
    18481 gggactctaa tcataaaaac ccatctcata aataacgtca tgcattacat gttaattatt
    18541 acatgcttaa cgtaattcaa cagaaattat atgataatca tcgcaagacc ggcaacagga
    18601 ttcaatctta agaaacttta ttgccaaatg tttgaacgat cgggaattgg atccggtacc
    18661 gagctcggat ccactagtaa cggccgccag tgtgctggaa ttcgcccttg gatccaccac
    18721 tttgtacaag aaagctgggt cggcgcgccc acccttttag ataacgatac cctccggagg
    18781 gttaaattct gctccaagat tgctgagctt cgcatgggct acagcgtaat ccttgaaaaa
    18841 tgcatcctga tctgcagcat acttttcagc atagaccttg aaagaagaat cttcgaagat
    18901 ggcagcatca gtgggtagga caaggagatc ttcatctctc ttttccttga tctccttgaa
    18961 gtaagaatta tcaaacttca gccactctgg tgtccatgac tgtcctcctg gtgctccagg
    19021 tccttctttc gtgtacttcg tctcaggctt cccccaacca ctacgttctg gcctagatct
    19081 tcctaacgtg tgagcaccag ataatgcaac tatgtccttg tcatctaatc ccattctgta
    19141 aaaaacttct ctgagatgag tagcaggtga aggaggacca gcatctggaa gccttccttc
    19201 ttctggacaa tcctcaggac cagaggcatc aactctacca tatttcatcg gtattttcgg
    19261 tcctccagct tcctctatag cagtagcact agctaactgg aacaaatcag cataactgat
    19321 cccagagtat ttctctttga tatccttaat caagtttaaa gcattcacaa gaccagcgtt
    19381 agcagcatgt ttaagctcaa tatcgaatct caaacttcca ttagctccac ctctttgtgg
    19441 ccattcctta atgttcttgt tatacg
//
