LOCUS       sequence-180320-       10275 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For nucleus-specific turboRFP labeling in single cells, pK263 should
            be used with pK031..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10275)
  AUTHORS   Luo W, Mizuno H, Iwata R, Nakazawa S, Yasuda K, Itohara S, Iwasato T
  TITLE     Supernova: A Versatile Vector System for Single-Cell Labeling and 
            Gene Function Studies in vivo.
  JOURNAL   Sci Rep. 2016 Oct 24;6:35747. doi: 10.1038/srep35747.
  PUBMED    27775045
REFERENCE   2  (bases 1 to 10275)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-10-24"; volume: "6:35747. doi"; pages: " 
            10.1038/srep35747"
COMMENT     Sequence Label: pK263.CAG-loxP-stop-loxP-NLSturboRFP-ires-tTA-...
FEATURES             Location/Qualifiers
     source          1..10275
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        10..389
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        391..666
                     /label=chicken beta-actin promoter
     intron          667..1679
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1603..1625
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1687..1706
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    complement(1736..1769)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     intron          2303..2368
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             2498..2518
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    2943..3077
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2980..2999)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3034..3053
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    complement(3119..3152)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             3168..3188
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3192..3884
                     /codon_start=1
                     /product="red fluorescent protein from Entacmaea 
                     quadricolor"
                     /label=TurboRFP
                     /note="mammalian codon-optimized"
                     /translation="SELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMKIKVV
                     EGGPLPFAFDILATSFMYGSKAFINHTQGIPDFFKQSFPEGFTWERITTYEDGGVLTAT
                     QDTSFQNGCIIYNVKINGVNFPSNGPVMQKKTRGWEANTEMLYPADGGLRGHSQMALKL
                     VGGGYLHCSFKTTYRSKKPAKNLKMPGFHFVDHRLERIKEADKETYVEQHEMAVAKYCD
                     LPSKLGHR"
     primer_bind     complement(4094..4111)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4321..4340
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(4493..5110,5111..5116,5117..5152,5153..5155,5156..5191,
                     5192..5194,5195..5230,5231..5239)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=tTA-Advanced
                     /note="In the Tet-Off(R) system, tTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the absence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(5326..5346)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5744..5755)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5884..5903)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    5949..6004
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6003..6022)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     rep_origin      complement(6388..6976)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6468..6487)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    7305..7352
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     7377..7396
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(7584..8378)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(7584..8378)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7695..7714)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     8305..8324
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     complement(8477..8496)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     enhancer        8575..8766
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     8723..8743
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     CDS             complement(join(8826..9617,9618..9686))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9449..9468
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9687..9791)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      9818..10273
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9905..9924)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10115..10136
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gcgcgcgttg acattgatta ttgactagtt attaatagta atcaattacg gggtcattag
       61 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
      121 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
      181 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
      241 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
      301 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
      361 tctacgtatt agtcatcgct attaccatgg tcgaggtgag ccccacgttc tgcttcactc
      421 tccccatctc ccccccctcc ccacccccaa ttttgtattt atttattttt taattatttt
      481 gtgcagcgat gggggcgggg gggggggggg cgcgcgccag gcggggcggg gcggggcgag
      541 gggcggggcg gggcgaggcg gagaggtgcg gcggcagcca atcagagcgg cgcgctccga
      601 aagtttcctt ttatggcgag gcggcggcgg cggcggccct ataaaaagcg aagcgcgcgg
      661 cgggcgggag tcgctgcgtt gccttcgccc cgtgccccgc tccgcgccgc ctcgcgccgc
      721 ccgccccggc tctgactgac cgcgttactc ccacaggtga gcgggcggga cggcccttct
      781 cctccgggct gtaattagcg cttggtttaa tgacggctcg tttcttttct gtggctgcgt
      841 gaaagcctta aagggctccg ggagggccct ttgtgcgggg gggagcggct cggggggtgc
      901 gtgcgtgtgt gtgtgcgtgg ggagcgccgc gtgcggcccg cgctgcccgg cggctgtgag
      961 cgctgcgggc gcggcgcggg gctttgtgcg ctccgcgtgt gcgcgagggg agcgcggccg
     1021 ggggcggtgc cccgcggtgc gggggggctg cgaggggaac aaaggctgcg tgcggggtgt
     1081 gtgcgtgggg gggtgagcag ggggtgtggg cgcggcggtc gggctgtaac ccccccctgc
     1141 acccccctcc ccgagttgct gagcacggcc cggcttcggg tgcggggctc cgtgcggggc
     1201 gtggcgcggg gctcgccgtg ccgggcgggg ggtggcggca ggtgggggtg ccgggcgggg
     1261 cggggccgcc tcgggccggg gagggctcgg gggaggggcg cggcggcccc cggagcgccg
     1321 gcggctgtcg aggcgcggcg agccgcagcc attgcctttt atggtaatcg tgcgagaggg
     1381 cgcagggact tcctttgtcc caaatctgtg cggagccgaa atctgggagg cgccgccgca
     1441 ccccctctag cgggcgcggg gcgaagcggt gcggcgccgg caggaaggaa atgggcgggg
     1501 agggccttcg tgcgtcgccg cgccgccgtc cccttctccc tctccagcct cggggctgtc
     1561 cgcgggggga cggctgcctt cgggggggac ggggcagggc ggggttcggc ttctggcgtg
     1621 tgaccggcgg ctctagagcc tctgctaacc atgttcatgc cttcttcttt ttcctacagc
     1681 tcctgggcaa cgtgctggtt attgtgctgt ctcatcattt tggcaaagaa ttctaataac
     1741 ttcgtatagc atacattata cgaagttata ttaagggttc cggatcctcg gggacaccaa
     1801 atatggcgat ctcggccttt tcgtttcttg gagctgggac atgtttgcca tcgatccatc
     1861 taccaccaga acggccgtta gatctgctgc caccgttgtt tccaccgaag aaaccaccgt
     1921 tgccgtaacc accacgacgg ttgttgctaa agaagctgcc accgccacgg ccaccgttgt
     1981 agccgccgtt gttgttattg tagttgctac tgttatttct ggcacttctt ggttttcctc
     2041 ttaagtgagg aggaacataa ccattctcgt tgttgtcgtt gatgcttaaa ttttgcactt
     2101 gttcgctcag ttcagccata atatgaaatg cttttcttgt tgttcttacg gaataccact
     2161 tgccacctat caccacaact aactttttcc cgttcctcca tctcttttat attttttttc
     2221 tcgagggatc tttgtgaagg aaccttactt ctgtggtgtg acataattgg acaaactacc
     2281 tacagagatt taaagctcta aggtaaatat aaaattttta agtgtataat gtgttaaact
     2341 actgattcta attgtttgtg tattttagat tccaacctat ggaactgatg aatgggagca
     2401 gtggtggaat gcctttaatg aggaaaacct gttttgctca gaagaaatgc catctagtga
     2461 tgatgaggct actgctgact ctcaacattc tactcctcca aaaaagaaga gaaaggtaga
     2521 agaccccaag gactttcctt cagaattgct aagttttttg agtcatgctg tgtttagtaa
     2581 tagaactctt gcttgctttg ctatttacac cacaaaggaa aaagctgcac tgctatacaa
     2641 gaaaattatg gaaaaatatt ctgtaacctt tataagtagg cataacagtt ataatcataa
     2701 catactgttt tttcttactc cacacaggca tagagtgtct gctattaata actatgctca
     2761 aaaattgtgt acctttagct ttttaatttg taaaggggtt aataaggaat atttgatgta
     2821 tagtgccttg actagagatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt
     2881 taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg
     2941 ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     3001 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     3061 cttatcatgt ctggatctga catggtaagt aagcttgggc tgcaggtcga gggacctaat
     3121 aacttcgtat agcatacatt atacgaagtt atatgtcgac catgggccca aagaagaaga
     3181 gaaaggtttc gagcgagctg atcaaggaga acatgcacat gaagctgtac atggagggca
     3241 ccgtgaacaa ccaccacttc aagtgcacat ccgagggcga aggcaagccc tacgagggca
     3301 cccagaccat gaagatcaag gtggtcgagg gcggccctct ccccttcgcc ttcgacatcc
     3361 tggctaccag cttcatgtac ggcagcaaag ccttcatcaa ccacacccag ggcatccccg
     3421 acttctttaa gcagtccttc cctgagggct tcacatggga gagaatcacc acatacgaag
     3481 acgggggcgt gctgaccgct acccaggaca ccagcttcca gaacggctgc atcatctaca
     3541 acgtcaagat caacggggtg aacttcccat ccaacggccc tgtgatgcag aagaaaacac
     3601 gcggctggga ggccaacacc gagatgctgt accccgctga cggcggcctg agaggccaca
     3661 gccagatggc cctgaagctc gtgggcgggg gctacctgca ctgctccttc aagaccacat
     3721 acagatccaa gaaacccgct aagaacctca agatgcccgg cttccacttc gtggaccaca
     3781 gactggaaag aatcaaggag gccgacaaag agacctacgt cgagcagcac gagatggctg
     3841 tggccaagta ctgcgacctc cctagcaaac tggggcacag atgatgcaga tatcctagat
     3901 cgagttaatt aaggattccg cccccccccc tctccctccc ccccccctaa cgttactggc
     3961 cgaagccgct tggaataagg ccggtgtgcg tttgtctata tgttattttc caccatattg
     4021 ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg tcttcttgac gagcattcct
     4081 aggggtcttt cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca
     4141 gttcctctgg aagcttcttg aagacaaaca acgtctgtag cgaccctttg caggcagcgg
     4201 aaccccccac ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata agatacacct
     4261 gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga tagttgtgga aagagtaaaa
     4321 tggctctcct caagcgtatt caacaagggg ctgaaggatg cccagaaggt accccattgt
     4381 atgggatctg atctggggcc tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa
     4441 aaacgtctag gccccccgaa ccacggggac gtggtttcct ttgaaaaaca cgatgtctag
     4501 actggacaag agcaaagtca taaactctgc tctggaatta ctcaatgaag tcggtatcga
     4561 aggcctgacg acaaggaaac tcgctcaaaa gctgggagtt gagcagccta ccctgtactg
     4621 gcacgtgaag aacaagcggg ccctgctcga tgccctggca atcgagatgc tggacaggca
     4681 tcatacccac ttctgccccc tggaaggcga gtcatggcaa gactttctgc ggaacaacgc
     4741 caagtcattc cgctgtgctc tcctctcaca tcgcgacggg gctaaagtgc atctcggcac
     4801 ccgcccaaca gagaaacagt acgaaaccct ggaaaatcag ctcgcgttcc tgtgtcagca
     4861 aggcttctcc ctggagaacg cactgtacgc tctgtccgcc gtgggccact ttacactggg
     4921 ctgcgtattg gaggatcagg agcatcaagt agcaaaagag gaaagagaga cacctaccac
     4981 cgattctatg cccccacttc tgagacaagc aattgagctg ttcgaccatc agggagccga
     5041 acctgccttc cttttcggcc tggaactaat catatgtggc ctggagaaac agctaaagtg
     5101 cgaaagcggc gggccggccg acgcccttga cgattttgac ttagacatgc tcccagccga
     5161 tgcccttgac gactttgacc ttgatatgct gcctgctgac gctcttgacg attttgacct
     5221 tgacatgctc cccgggtaac taagtaagcg gccgcgaatt cgcccttcgg ccgctcaacc
     5281 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac
     5341 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt
     5401 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt
     5461 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg
     5521 cattgccacc acctgtcagc tcctttccgg aactttcgct ttccccctcc ctattgccac
     5581 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac
     5641 tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctatgt
     5701 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc
     5761 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg
     5821 ccctcagacg agtcggatct ccctttgggc cgcctccccg cagcggccgc ggcgactcga
     5881 ggatcttttt ccctctgcca aaaattatgg ggacatcatg aagccccttg agcatctgac
     5941 ttctggctaa taaaggaaat ttattttcat tgcaatagtg tgttggaatt ttttgtgtct
     6001 ctcactcgga aggacatatg ggagggcaaa tcatttaaaa catcagaatg agtatttggt
     6061 ttagagtttg gcaacatatg cccatatgct ggctgccatg aacaaaggtt ggctataaag
     6121 aggtcatcag tatatgaaac agccccctgc tgtccattcc ttattccata gaaaagcctt
     6181 gacttgaggt tagatttttt tatattttgt tttgtgttat ttttttcttt aacatcccta
     6241 aaattttcct tacatgtttt actagccaga tttttcctcc tctcctgact actcccagtc
     6301 atagctgtcc ctcttctctt atgaagccat gtgagcaaaa ggccagcaaa aggccaggaa
     6361 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     6421 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     6481 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     6541 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     6601 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     6661 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     6721 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     6781 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     6841 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     6901 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     6961 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     7021 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     7081 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aacctgaggc
     7141 tatggcaggg cctgccgccc cgacgttggc tgcgagccct gggccttcac ccgaacttgg
     7201 ggggtggggt ggggaaaagg aagaaacgcg ggcgtattgg ccccaatggg gtctcggtgg
     7261 ggtatcgaca gagtgccagc cctgggaccg aaccccgcgt ttatgaacaa acgacccaac
     7321 accgtgcgtt ttattctgtc tttttattgc cgtcatagcg cgggttcctt ccggtattgt
     7381 ctccttccgt gtttcagtta gcctccccct agggtgggcg aagaactcca gcatgagatc
     7441 cccgcgctgg aggatcatcc agccggcgtc ccggaaaacg attccgaagc ccaacctttc
     7501 atagaaggcg gcggtggaat cgaaatctcg tgatggcagg ttgggcgtcg cttggtcggt
     7561 catttcgaac cccagagtcc cgctcagaag aactcgtcaa gaaggcgata gaaggcgatg
     7621 cgctgcgaat cgggagcggc gataccgtaa agcacgagga agcggtcagc ccattcgccg
     7681 ccaagctctt cagcaatatc acgggtagcc aacgctatgt cctgatagcg gtccgccaca
     7741 cccagccggc cacagtcgat gaatccagaa aagcggccat tttccaccat gatattcggc
     7801 aagcaggcat cgccatgggt cacgacgaga tcctcgccgt cgggcatgct cgccttgagc
     7861 ctggcgaaca gttcggctgg cgcgagcccc tgatgctctt cgtccagatc atcctgatcg
     7921 acaagaccgg cttccatccg agtacgtgct cgctcgatgc gatgtttcgc ttggtggtcg
     7981 aatgggcagg tagccggatc aagcgtatgc agccgccgca ttgcatcagc catgatggat
     8041 actttctcgg caggagcaag gtgagatgac aggagatcct gccccggcac ttcgcccaat
     8101 agcagccagt cccttcccgc ttcagtgaca acgtcgagca cagctgcgca aggaacgccc
     8161 gtcgtggcca gccacgatag ccgcgctgcc tcgtcttgca gttcattcag ggcaccggac
     8221 aggtcggtct tgacaaaaag aaccgggcgc ccctgcgctg acagccggaa cacggcggca
     8281 tcagagcagc cgattgtctg ttgtgcccag tcatagccga atagcctctc cacccaagcg
     8341 gccggagaac ctgcgtgcaa tccatcttgt tcaatcatgc gaaacgatcc tcatcctgtc
     8401 tcttgatcga tctttgcaaa agcctaggcc tccaaaaaag cctcctcact acttctggaa
     8461 tagctcagag gccgggcctc ggcctctgca taaataaaaa aaattagtca gccatggggc
     8521 ggagaatggg cggaactggg cggagttagg ggcgggatgg gcggagttag gggcgggact
     8581 atggttgctg actaattgag atgcatgctt tgcatacttc tgcctgctgg ggagcctggg
     8641 gactttccac acctggttgc tgactaattg agatgcatgc tttgcatact tctgcctgct
     8701 ggggagcctg gggactttcc acaccctaac tgacacacat tccacagctg gttctttccg
     8761 cctcaggact cttccttttt caataaatca atctaaagta tatatgagta aacttggtct
     8821 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     8881 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     8941 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     9001 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     9061 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     9121 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     9181 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     9241 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     9301 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     9361 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     9421 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     9481 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     9541 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     9601 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     9661 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     9721 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     9781 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg cgccctgtag cggcgcatta
     9841 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     9901 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     9961 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
    10021 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
    10081 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
    10141 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
    10201 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
    10261 acgcttacaa tttac
//
