LOCUS       sequence-180324-        8815 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector with dox inducible expression of shRNA targeting 
            human FOXM1..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8815)
  TITLE     FOXM1 toolbox
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8815)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: Tet-pLKO-FOXM1-shRNA-#2
FEATURES             Location/Qualifiers
     source          1..8815
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(17..36)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     246..263
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     CDS             complement(465..1085)
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     993..1012
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     promoter        complement(1096..1114)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1142..1161)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     intron          1169..1741
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     promoter        complement(1750..2260)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(1813..1831)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     misc_feature    2309..2426
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        complement(2526..2620)
                     /label=H1-2O2 promoter
                     /note="doxycycline-inducible variant of the human H1 RNA 
                     promoter (Henriksen et al., 2007)"
     protein_bind    2526..2544
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2554..2572
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(2590..2609)
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     CDS             complement(2909..2953)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3138..3371
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    3864..3989
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             4036..4216
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(4217..4443)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(4471..4491)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(4471..4489)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4510..4526)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4510..4526)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4523..4545)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4534..4550
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4558..4564,4565..4582,4583..4588))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4603..4624
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4741..4758)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4912..5500)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4992..5011)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5671..6462,6463..6531))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6294..6313
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6532..6636)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(6662..7117)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6799..6820)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7011..7030
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7244..7266
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7258..7275
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7259..7275
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        7285..7303
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      7324..7459
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    7499..7620
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7510..7529)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7564..7583
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             7692..7925
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     CDS             complement(8053..8652)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8136..8156)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     8633..8652
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     misc_feature    join(8672..8815,1..430)
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
ORIGIN
        1 cttcagcccc ttgttgaata cgcttgagga gagccatttg actctttcca caactatcca
       61 actcacaacg tggcactggg gttgtgccgc ctttgcaggt gtatcttata cacgtggctt
      121 ttggccgcag aggcacctgt cgccaggtgg ggggttccgc tgcctgcaaa gggtcgctac
      181 agacgttgtt tgtcttcaag aagcttccag aggaactgct tccttcacga cattcaacag
      241 accttgcatt cctttggcga gaggggaaag acccctagga atgctcgtca agaagacagg
      301 gccaggtttc cgggccctca cattgccaaa agacggcaat atggtggaaa ataacatata
      361 gacaaacgca caccggcctt attccaagcg gcttcggcca gtaacgttag ggggggggga
      421 gggagagggg cagatcccta gaactcccgg gagccgctgt acgcggaccc actttcacat
      481 ttaagttgtt tttctaatcc gcatatgatc aattcaaggc cgaataagaa ggctggctct
      541 gcaccttggt gatcaaataa ttcgatagct tgtcgtaata atggcggcat actatcagta
      601 gtaggtgttt ccctttcttc tttagcgact tgatgctctt gatcttccaa tacgcaacct
      661 aaagtaaaat gccccacagc gctgagtgca tataatgcat tctctagtga aaaaccttgt
      721 tggcataaaa aggctaattg attttcgaga gtttcatact gtttttctgt aggccgtgta
      781 cctaaatgta cttttgctcc atcgcgatga cttagtaaag cacatctaaa acttttagcg
      841 ttattacgta aaaaatcttg ccagctttcc ccttctaaag ggcaaaagtg agtatggtgc
      901 ctatctaaca tctcaatggc taaggcgtcg agcaaagccc gcttattttt tacatgccaa
      961 tacaatgtag gctgctctac acctagcttc tgggcgagtt tacgggttgt taaaccttcg
     1021 attccgacct cattaagcag ctctaatgcg ctgttaatca ctttactttt atctaatcta
     1081 gacatatcaa ttcgccctat agtgagtcgt attacaattc tttgccaaaa tgatgagaca
     1141 gcacaataac cagcacgttg cccaggagct gtaggaaaaa gaagaaggca tgaacatggt
     1201 tagcagaggg gcccggtttg gactcagagt attttatcct catctcaaac agtgtatatc
     1261 attgtaacca taaagagaaa ggcaggatga tgaccaggat gtagttgttt ctaccaataa
     1321 gaatatttcc acgccagcca gaatttatat gcagaaatat tctaccttat catttaatta
     1381 taacaattgt tctctaaaac tgtgctgaag tacaatataa tataccctga ttgccttgaa
     1441 aaaaaagtga ttagagaaag tacttacaat ctgacaaata aacaaaagtg aatttaaaaa
     1501 ttcgttacaa atgcaagcta aagtttaacg aaaaagttac agaaaatgaa aagaaaataa
     1561 gaggagacaa tggttgtcaa cagagtagaa agtgaaagaa acaaaattat catgagggtc
     1621 catggtgata caagggacat cttcccattc taaacaacac cctgaaaact ttgccccctc
     1681 catataacat gaattttaca atagcgaaaa agaaagaaca atcaagggtc cccaaactca
     1741 cagatccccc tggggagaga ggtcggtgat tcggtcaacg agggagccga ctgccgacgt
     1801 gcgctccgga ggcttgcaga atgcggaaca ccgcgcgggc aggaacaggg cccacactac
     1861 cgccccacac cccgcctccc gcaccgcccc ttcccggccg ctgctctcgg cgcgccctgc
     1921 tgagcagccg ctattggcca cagcccatcg cggtcggcgc gctgccattg ctccctggcg
     1981 ctgtccgtct gcgagggtac tagtgagacg tgcggcttcc gtttgtcacg tccggcacgc
     2041 cgcgaaccgc aaggaacctt cccgacttag gggcggagca ggaagcgtcg ccggggggcc
     2101 cacaagggta gcggcgaaga tccgggtgac gctgcgaacg gacgtgaaga atgtgcgaga
     2161 cccagggtcg gcgccgctgc gtttcccgga accacgccca gagcagccgc gtccctgcgc
     2221 aaacccaggg ctgccttgga aaaggcgcaa ccccaacccc ctcgagccgc ggccaaagtg
     2281 gatctctgct gtccctgtaa taaacccgaa aattttgaat ttttgtaatt tgtttttgta
     2341 attctttagt ttgtatgtct gttgctatta tgtctactat tctttcccct gcactgtacc
     2401 ccccaatccc cccttttctt ttaaaattgt ggatgaatac tgccatttgt ctcgaggtcg
     2461 agaattcaaa aacctttccc tgcacgacat gtctcttgaa catgtcgtgc agggaaaggc
     2521 cggtgtctct atcactgata gggaacttat aagtctctat cactgatagg gatttcacgt
     2581 ttatggtgat ttcccagaac acatagcgac atgcaaatat tgcggccgct cgaggctagt
     2641 ctcgtgatcg ataccgtcga gatccgttca ctaatcgaat ggatctgtct ctgtctctct
     2701 ctccaccttc ttcttctatt ccttcgggcc tgtcgggtcc cctcggggtt gggaggtggg
     2761 tctgaaacga taatggtgaa tatccctgcc taactctatt cactatagaa agtacagcaa
     2821 aaactattct taaacctacc aagcctccta ctatcattat gaataatttt atataccaca
     2881 gccaatttgt tatgttaaac caattccaca aacttgccca tttatctaat tccaataatt
     2941 cttgttcatt cttttcttgc tggttttgcg attcttcaat taaggagtgt attaagcttg
     3001 tgtaattgtt aatttctctg tcccactcca tccaggtcgt gtgattccaa atctgttcca
     3061 gagatttatt actccaacta gcattccaag gcacagcagt ggtgcaaatg agttttccag
     3121 agcaacccca aatccccagg agctgttgat cctttaggta tctttccaca gccaggattc
     3181 ttgcctggag ctgcttgatg ccccagactg tgagttgcaa cagatgctgt tgcgcctcaa
     3241 tagccctcag caaattgttc tgctgctgca ctataccaga caataattgt ctggcctgta
     3301 ccgtcagcgt cattgacgct gcgcccatag tgcttcctgc tgctcccaag aacccaagga
     3361 acaaagctcc tattcccact gctctttttt ctctctgcac cactcttctc tttgccttgg
     3421 tgggtgctac tcctaatggt tcaattttta ctactttata tttatataat tcacttctcc
     3481 aattgtccct catatctcct cctccaggtc tgaagatcag cggccgcttg ctgtgcggtg
     3541 gtcttacttt tgttttgctc ttcctctatc ttgtctaaag cttccttggt gtcttttatc
     3601 tctatccttt gatgcacaca atagagggtt gctactgtat tatataatga tctaagttct
     3661 tctgatcctg tctgaaggga tggttgtagc tgtcccagta tttgtctaca gccttctgat
     3721 gtttctaaca ggccaggatt aactgcgaat cgttctagct ccctgcttgc ccatactata
     3781 tgttttaatt tatatttttt ctttccccct ggccttaacc gaattttttc ccatcgcgat
     3841 ctaattctcc cccgcttaat actgacgctc tcgcacccat ctctctcctt ctagcctccg
     3901 ctagtcaaaa tttttggcgt actcaccagt cgccgcccct cgcctcttgc cgtgcgcgct
     3961 tcagcaagcc gagtcctgcg tcgagagagc tcctctggtt tccctttcgc tttcaagtcc
     4021 ctgttcgggc gccactgcta gagattttcc acactgacta aaagggtctg agggatctct
     4081 agttaccaga gtcacacaac agacgggcac acactacttg aagcactcaa ggcaagcttt
     4141 attgaggctt aagcagtggg ttccctagtt agccagagag ctcccaggct cagatctggt
     4201 ctaaccagag agacccgttt atgtatcgag ctaggcactt aaatacaata tctctgcaat
     4261 gcggcaattc agtggttcgt ccaatccatg tcagacccgt ctgttgcctt cctaataagg
     4321 cacgatcgta ccaccttact tccaccaatc ggcatgcacg gtgctttttc tctccttgta
     4381 aggcatgttg ctaactcatc gttaccatgt tgcaagacta caagagtatt gcataagact
     4441 acattaagct tgcagctcca gcttttgttc cctttagtga gggttaattg cgcgcttggc
     4501 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     4561 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     4621 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     4681 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     4741 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     4801 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     4861 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     4921 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     4981 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     5041 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     5101 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     5161 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     5221 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     5281 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     5341 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     5401 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     5461 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     5521 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     5581 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     5641 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     5701 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     5761 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     5821 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     5881 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     5941 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     6001 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     6061 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     6121 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     6181 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     6241 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     6301 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     6361 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     6421 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     6481 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     6541 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     6601 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     6661 taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca
     6721 ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag
     6781 atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc
     6841 aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc
     6901 taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc
     6961 ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa
     7021 gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc
     7081 acacccgccg cgcttaatgc gccgctacag ggcgcgtccc attcgccatt caggctgcgc
     7141 aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg
     7201 ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt
     7261 aaaacgacgg ccagtgagcg cgcgtaatac gactcactat agggcgaatt gggtacgtcc
     7321 ctaggcctcc aaaaaagcct cctcactact tctggaatag ctcagaggcc gaggcggcct
     7381 cggcctctgc ataaataaaa aaaattagtc agccatgggg cggagaatgg gcggaactgg
     7441 gcggagttag gggcgggatg ggcggagtta ggggcgggat agctagagcc agacatgata
     7501 agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt
     7561 tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt
     7621 cctctcactc tctgatattc atttctttgc aagttataaa tactgaataa taagatgaca
     7681 tgaactacta ctgctagaga ttttccacac tgactaaaag ggtctgaggg atctctagtt
     7741 accagagtca cacaacagac gggcacacac tacttgaagc actcaaggca agctttattg
     7801 aggcttaagc agtgggttcc ctagttagcc agagagctcc caggctcaga tctggtctaa
     7861 ccagagagac ccagtacaag caaaaagcag atcttgtctt cgttgggagt gaattagccc
     7921 ttccagtccc cccttttctt ttaaaaagtg gctaagatct acagctgcct tgtaagtcat
     7981 tggtcttaaa ggtaccgatg catggggtcg tgcgctcctt tcggtcgggc gctgcgggtc
     8041 gtggggcggg cgtcaggcac cgggcttgcg ggtcatgcac caggtgcgcg gtccttcggg
     8101 cacctcgacg tcggcggtga cggtgaagcc gagccgctcg tagaagggga ggttgcgggg
     8161 cgcggaggtc tccaggaagg cgggcacccc ggcgcgctcg gccgcctcca ctccggggag
     8221 cacgacggcg ctgcccagac ccttgccctg gtggtcgggc gagacgccga cggtggccag
     8281 gaaccacgcg ggctccttgg gccggtgcgg cgccaggagg ccttccatct gttgctgcgc
     8341 ggccagccgg gaaccgctca actcggccat gcgcgggccg atctcggcga acaccgcccc
     8401 cgcttcgacg ctctccggcg tggtccagac cgccaccgcg gcgccgtcgt ccgcgaccca
     8461 caccttgccg atgtcgagcc cgacgcgcgt gaggaagagt tcttgcagct cggtgacccg
     8521 ctcgatgtgg cggtccggat cgacggtgtg gcgcgtggcg gggtagtcgg cgaacgcggc
     8581 ggcgagggtg cgtacggccc tggggacgtc gtcgcgggtg gcgaggcgca ccgtgggctt
     8641 gtactcggtc atggtaagct ccggtggatc cttatcatcg tgtttttcaa aggaaaacca
     8701 cgtccccgtg gttcgggggg cctagacgtt tttttaacct cgactaaaca catgtaaagc
     8761 atgtgcaccg aggccccaga tcagatccca tacaatgggg taccttctgg gcatc
//
