LOCUS       sequence-180327-        3854 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial expression vector for Trx1 SSAAA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3854)
  AUTHORS   Schwertassek U, Balmer Y, Gutscher M, Weingarten L, Preuss M, 
            Engelhard J, Winkler M, Dick TP
  TITLE     Selective redox regulation of cytokine receptor signaling by 
            extracellular thioredoxin-1.
  JOURNAL   EMBO J. 2007 Jul 11;26(13):3086-97. Epub 2007 Jun 7.
  PUBMED    17557078
REFERENCE   2  (bases 1 to 3854)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J."; 
            date: "2007-07-11"; volume: "26(13)"; pages: "3086-97. Epub 2007 Jun
            7"
COMMENT     Sequence Label: pQE60 Trx1 SSAAA
FEATURES             Location/Qualifiers
     source          1..3854
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(10..24,25..30,31..47,48..54)
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    30..46
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    62..78
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             97..108
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             436..549
                     /codon_start=1
                     /product="streptavidin-binding peptide"
                     /label=SBP
                     /note="selected from a peptide library; binds streptavidin 
                     with nanomolar affinity (Keefe et al., 2001)"
                     /translation="MDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREP"
     CDS             556..573
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      596..690
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     complement(800..819)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      1462..1548
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(1596..1618)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    1704..1844
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     1759..1778
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(1859..1876)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2030..2618)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2110..2129)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2789..3580,3581..3649))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3412..3431
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3650..3754)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3822..3840
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ctcgagaaat cataaaaaat ttatttgctt tgtgagcgga taacaattat aatagattca
       61 attgtgagcg gataacaatt tcacacagaa ttcattaaag aggagaaatt aaccatggtg
      121 aagcagatcg aaagcaagac tgcttttcag gaagccttgg acgctgcagg tgacaaactt
      181 gtagtagttg acttctcagc cacgtggagt gggccttcca aaatgatcaa gcctttcttt
      241 cattccctct ctgaaaagta ttccaacgtg atattccttg aagtagatgt ggatgacgct
      301 caggatgttg cttcagaggc tgaagtcaaa gccatgccaa cattccagtt ttttaagaag
      361 ggacaaaagg tgggtgaatt ttctggagcc aataaggaaa agcttgaagc caccattaat
      421 gaattagtca gatctatgga cgaaaagacc accggctggc gcggcggcca cgtggtggaa
      481 ggcctggccg gcgaactgga acagctgcgc gcccgcctgg aacaccaccc acagggccag
      541 cgcgaaccag gatctcatca ccatcaccat cactaagctt aattagctga gcttggactc
      601 ctgttgatag atccagtaat gacctcagaa ctccatctgg atttgttcag aacgctcggt
      661 tgccgccggg cgttttttat tggtgagaat ccaagctagc ttggcgagat tttcaggagc
      721 taaggaagct aaaatggaga aaaaaatcac tggatatacc accgttgata tatcccaatg
      781 gcatcgtaaa gaacattttg aggcatttca gtcagttgct caatgtacct ataaccagac
      841 cgttcagctg gatattacgg cctttttaaa gaccgtaaag aaaaataagc acaagtttta
      901 tccggccttt attcacattc ttgcccgcct gatgaatgct catccggaat ttcgtatggc
      961 aatgaaagac ggtgagctgg tgatatggga tagtgttcac ccttgttaca ccgttttcca
     1021 tgagcaaact gaaacgtttt catcgctctg gagtgaatac cacgacgatt tccggcagtt
     1081 tctacacata tattcgcaag atgtggcgtg ttacggtgaa aacctggcct atttccctaa
     1141 agggtttatt gagaatatgt ttttcgtctc agccaatccc tgggtgagtt tcaccagttt
     1201 tgatttaaac gtggccaata tggacaactt cttcgccccc gttttcacca tgcatgggca
     1261 aatattatac gcaaggcgac aaggtgctga tgccgctggc gattcaggtt catcatgccg
     1321 tctgtgatgg cttccatgtc ggcagaatgc ttaatgaatt acaacagtac tgcgatgagt
     1381 ggcagggcgg ggcgtaattt ttttaaggca gttattggtg cccttaaacg cctggggtaa
     1441 tgactctcta gcttgaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt
     1501 cgttttatct gttgtttgtc ggtgaacgct ctcctgagta ggacaaatcc gccgctctag
     1561 agctgcctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag
     1621 acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca
     1681 gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga tagcggagtg
     1741 tatactggct taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt
     1801 gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgctct tccgcttcct
     1861 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     1921 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     1981 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     2041 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     2101 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     2161 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     2221 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     2281 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     2341 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     2401 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     2461 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     2521 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     2581 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     2641 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     2701 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     2761 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     2821 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     2881 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     2941 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     3001 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     3061 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     3121 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     3181 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     3241 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     3301 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     3361 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     3421 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     3481 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     3541 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     3601 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     3661 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     3721 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     3781 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc
     3841 cctttcgtct tcac
//
