LOCUS       sequence-180360-       10216 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutively expresses estradiol-inducible synthetic 
            transcriptional regulator in yeast. Transcriptional regulator 
            activates transcription of promoters containing Gal4 binding sites..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10216)
  AUTHORS   Aranda-Diaz A, Mace K, Zuleta I, Harrigan P, El-Samad H
  TITLE     Robust Synthetic Circuits for Two-Dimensional Control of Gene 
            Expression in Yeast.
  JOURNAL   ACS Synth Biol. 2016 Dec 27. doi: 10.1021/acssynbio.6b00251.
  PUBMED    27930885
REFERENCE   2  (bases 1 to 10216)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 Dec 27. doi: 10.1021/acssynbio.6b00251."
COMMENT     Sequence Label: pHES839
FEATURES             Location/Qualifiers
     source          1..10216
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    12..30
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    33..51
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             104..544
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECCYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLGMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     CDS             590..1525
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     CDS             638..1528
                     /codon_start=1
                     /gene="ESR1"
                     /product="estrogen nuclear receptor alpha ligand-binding 
                     domain"
                     /label=ER-LBD
                     /translation="KRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMM
                     GLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPVKL
                     LFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTF
                     LSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNK
                     GMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSL
                     QKYYITGEAEGFPATV"
     primer_bind     complement(3707..3726)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3708..3726)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3733..3750)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3733..3749)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3742..3764)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3890..4345
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3977..3996)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4187..4208
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4701..4720)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4820..4842
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4880..4898)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4983..5087
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5088..5156,5157..5948)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5306..5325)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6119..6707
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6608..6627
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6861..6878
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6995..7016
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7031..7036,7037..7054,7055..7061)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7069..7085
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7074..7096
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7093..7109
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7093..7109
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        7130..7148
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7436..7843
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
ORIGIN
        1 atagacctga atccctatca gtgatagaga tctccctatc agtgatagag attcttcctt
       61 gtttcttttt ctgcaggtcg acaaacaaat gctcgacata aaaatgaagc tactgtcttc
      121 tatcgaacaa gcatgcgata tttgccgact taaaaagctc aagtgctcca aagaaaaacc
      181 gaagtgcgcc aagtgtctga agaacaactg ggagtgttgc tactctccca aaaccaaaag
      241 gtctccgctg actagggcac atctgacaga agtggaatca aggctagaaa gactggaaca
      301 gctatttcta ctgatttttc ctcgagaaga ccttggcatg attttgaaaa tggattcttt
      361 acaggatata aaagcattgt taacaggatt atttgtacaa gataatgtga ataaagatgc
      421 cgtcacagat agattggctt cagtggagac tgatatgcct ctaacattga gacagcatag
      481 aataagtgcg acatcatcat cggaagagag tagtaacaaa ggtcaaagac agttgactgt
      541 atcgattgac tcggcaggat ccggtgacgg tgctggttta attaactctg ctggagacat
      601 gagagctgcc aacctttggc caagcccgct catgatcaaa cgctctaaga agaacagcct
      661 ggccttgtcc ctgacggccg accagatggt cagtgccttg ttggatgctg agccccccat
      721 actctattcc gagtatgatc ctaccagacc cttcagtgaa gcttcgatga tgggcttact
      781 gaccaacctg gcagacaggg agctggttca catgatcaac tgggcgaaga gggtgccagg
      841 ctttgtggat ttgaccctcc atgatcaggt ccaccttcta gaatgtgcct ggctagagat
      901 cctgatgatt ggtctcgtct ggcgctccat ggagcaccca gtgaagctac tgtttgctcc
      961 taacttgctc ttggacagga accagggaaa atgtgtagag ggcatggtgg agatcttcga
     1021 catgctgctg gctacatcat ctcggttccg catgatgaat ctgcagggag aggagtttgt
     1081 gtgcctcaaa tctattattt tgcttaattc tggagtgtac acatttctgt ccagcaccct
     1141 gaagtctctg gaagagaagg accatatcca ccgagtcctg gacaagatca cagacacttt
     1201 gatccacctg atggccaagg caggcctgac cctgcagcag cagcaccagc ggctggccca
     1261 gctcctcctc atcctctccc acatcaggca catgagtaac aaaggcatgg agcatctgta
     1321 cagcatgaag tgcaagaacg tggtgcccct ctatgacctg ctgctggaga tgctggacgc
     1381 ccaccgccta catgcgccca ctagccgtgg aggggcatcc gtggaggaga cggaccaaag
     1441 ccacttggcc actgcgggct ctacttcatc gcattccttg caaaagtatt acatcacggg
     1501 ggaggcagag ggtttccctg ccacagtcgc ggccgcaggt gacggtgctg gtttaattaa
     1561 catgacggtc gaccatgatt tcaatagcga agatatttta ttccccatag aaagcatgag
     1621 tagtatacaa tacgtggaga ataataaccc aaataatatt aacaacgatg ttatcccgta
     1681 ttctctagat atcaaaaaca ctgtcttaga tagtgcggat ctcaatgaca ttcaaaatca
     1741 agaaacttca ctgaatttgg ggcttcctcc actatctttc gactctccac tgcccgtaac
     1801 ggaaacgata ccatccacta ccgataacag cttgcatttg aaagctgata gcaacaaaaa
     1861 tcgcgatgca agaactattg aaaatgatag tgaaattaag agtactaata atgctagtgg
     1921 ctctggggca aatcaataca caactcttac ttcaccttat cctatgaacg acattttgta
     1981 caacatgaac aatccgttac aatcaccgtc accttcatcg gtacctcaaa atccgactat
     2041 aaatcctccc ataaatacag caagtaacga aactaattta tcgcctcaaa cttcaaatgg
     2101 taatgaaact cttatatctc ctcgagccca acaacatacg tccattaaag ataatcgtct
     2161 gtccttacct aatggtgcta attcgaatct tttcattgac actaacccaa acaatttgaa
     2221 cgaaaaacta agaaatcaat tgaactcaga tacaaattca tattctaact ccatttctaa
     2281 ttcaaactcc aattctacgg gtaatttaaa ttccagttat tttaattcac tgaacataga
     2341 ctccatgcta gatgattacg tttctagtga tctcttattg aatgatgatg atgatgacac
     2401 taatttatca cgccgaagat ttagcgacgt tataacaaac caagagctct aagcaaatag
     2461 ctaaattata tacgaattaa tattatgatt aagtgtttac gtgagtgcga tatttttatt
     2521 actatcttat acagttgtat atactctata aaatgagttg tctattaatt aacgcgatga
     2581 atgctttctg ggtttacctc tccaacaact ctagtttact tctcaataca ttcaattgta
     2641 tttgatttgt caatacttca tcattaatca attctatagt tttgtttttc tcgtttattt
     2701 ccaaatttaa tgcatcaatt ttattattca atttgtcgtt gattttggtt aatgatttta
     2761 tggtttgatc tctggcattg attgtttgtg ttagtttttc attattgata attaaattat
     2821 ttaagttagt tatcaactcg gtgttttcaa gtttcaagtt ttcaatttct ttagagttta
     2881 ttagatttgt caaagtttct gaattgcttg attggtcctg tagaagagta tttgttgttg
     2941 tggataattg attcaatttt tgagacaatt gctggaaggc gttgaaatat ctagcatcaa
     3001 tctcatggtt tttttcccga gagtctcgta gattcaattg ttttaatata tcttgggacc
     3061 actcttgatt tgaactcatg gaaattaaac tgggtgttgt gttgtggtgt aatgattgta
     3121 ccccctttgc ttataattgt gtggccaccg aagtcggtga tgctgtcgcc gaagaagtta
     3181 agaaaatcct tgcttaaaaa gattctcttt ttttatgata tttgtacata aactttataa
     3241 atgaaattca taatagaaac gacacgaaat tacaaaatgg aatatgttca tagggtagac
     3301 gaaactatat acgcaatcta catacattta tcaagaagga gaaaaaggag gatgtaaagg
     3361 aatacaggta agcaaattga tactaatggc tcaacgtgat aaggaaaaag aattgcactt
     3421 taacattaat attgacaagg aggagggcac cacacaaaaa gttaggtgta acagaaaatc
     3481 atgaaactat gattcctaat ttatatattg gaggattttc tctaaaaaaa aaaaaataca
     3541 acaaataaaa aacactcaat gacctgacca tttgatggag tttaagtcaa tacctccttg
     3601 aaccatttcc cataatggtg aaagttccct caagaatttt actctgtcag aaacggcctt
     3661 aacgacgtag gccggccatt taaatgttta aacggccacg ctggccccct atagtgagtc
     3721 gtattacaat tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac
     3781 ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc
     3841 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatgga cgcgccctgt
     3901 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     3961 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     4021 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     4081 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     4141 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     4201 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     4261 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
     4321 aacaaaatat taacgcttac aatttcctga tgcggtattt tctccttacg catctgtgcg
     4381 gtatttcaca ccgcataggg taataactga tataattaaa ttgaagctct aatttgtgag
     4441 tttagtatta ggatctctac ccttggcgaa aagtcctctg ccaacaatga tgatatctga
     4501 tccaccactt acaacttcgt cgacggttct gtactgctga cccaatatgc gtatatatac
     4561 caatctaagt ctgtgctcct tccttcgttc ttccttctgt tcggagatta ccgaatcaaa
     4621 aaaatttcaa ggaaaccgaa atcaaaaaaa agaataaaaa aaaaatgatg aattgaaaag
     4681 gtggtatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga
     4741 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac
     4801 agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg
     4861 aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
     4921 ataatggttt cttagacgtc ggcgcgccga ggacgtcagg tggcactttt cggggaaatg
     4981 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     5041 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     5101 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     5161 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     5221 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     5281 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     5341 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     5401 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     5461 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     5521 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     5581 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     5641 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     5701 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     5761 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     5821 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     5881 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     5941 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     6001 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     6061 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     6121 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     6181 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     6241 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     6301 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     6361 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     6421 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     6481 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     6541 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     6601 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     6661 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     6721 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     6781 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     6841 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     6901 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
     6961 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
     7021 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
     7081 aacaatttca cacaggaaac agctatgacc atgattacgc caagctcgga attaaccctc
     7141 actaaaggga acaaaagctg ggccggccat ttaaatgttt aaacggccac gctggccgag
     7201 gagaacttct agtatatcta catacctaat attattgcct tattaaaaat ggaatcccaa
     7261 cgattacatc aaaatccaca ttctcttcaa aatcaattgt cctgtacttc cttgttcatg
     7321 tgtgttcaaa aacgttatat ttataggata attatactct atttctcaac aagtaattgg
     7381 ttgtttggcc gagcggtcta aggcgcctga ttcaagaaat atcttgaccg cagttaactg
     7441 tgggaatact caggtatcgt aagatgcaag agttcgaatc tcttagcaac cattattttt
     7501 ttcctcaaca taacgagaac acacaggggc gctatcgcac agaatcaaat tcgatgactg
     7561 gaaatttttt gttaatttca gaggtcgcct gacgcatata cctttttcaa ctgaaaaatt
     7621 gggagaaaaa ggaaaggtga gagcgccgga accggctttt catatagaat agagaagcgt
     7681 tcatgactaa atgcttgcat cacaatactt gaagttgaca atattattta aggacctatt
     7741 gttttttcca ataggtggtt agcaatcgtc ttactttcta acttttctta ccttttacat
     7801 ttcagcaata tatatatata tatttcaagg atataccatt ctaatgtctg cccctaagtt
     7861 accaatagat agcggtttaa tataaggttt caacaagaaa tattttgtga aaatcaaaag
     7921 ttaatagttt cactgtcgaa aatatgaaag tttaagtttt cagttcatcc cattgtagtt
     7981 atcaaatttc tgtgtttccc gtatatgtgt gctctgtatt catcgctatc ttgaaactgg
     8041 ttatgatcgt tgcgcatgtg acctgtatcg ctcaaaagag cccggcaccg gtaccgggca
     8101 aaagccccta gcaagctaaa atgattcaac tttcgtagta atttgttcaa ccggtcacag
     8161 taaagtcaat gactaacttt tgaatcgagg atgtatttcc aattgaaagc aatgtatata
     8221 taaaccattg tgttgaagct tgtcttacag ttttcttgta tcctcccatt gttcatatag
     8281 tacttaaagt accataatcc ttaaagaaca aacttgaaag agaatacact atacacaact
     8341 aataaataaa gaataatcat ggctgtgacc aagacaattg tagttctacc aggtgaccat
     8401 gttggtcaag aaatcactga agaggccatt aaagttttaa atgctattca ggaatgtcgt
     8461 ccagacaagg tcaatttcaa gtttgatcat catttgatcg gtggtgctgc aattgatgcc
     8521 actggtgttc cattaccaga cgaagctttg gaggcctcta agaaagctga tgccgtgctt
     8581 ctgggtgctg ttggtggtcc aaaatggggt actggcgctg tcagaccaga acaaggtctt
     8641 ttgaaaatcc gtaaggagtt gcaattgtat gctaatctaa gaccatgtaa ttttgcatct
     8701 gattccttac tagatctatc gccattgaag cctgaaattg caagaggtac agatttcgtc
     8761 gttgttagag aactagtggg tggtatttat ttcggggaga gaaaagaaga tgaaggtgat
     8821 ggtgtcgcct gggatagcga aaagtattct gtgcccgaag ttcaaagaat cactagaatg
     8881 gctgctttca tggctctgca acacaaccca cctttaccta tttggtctct agataaagcc
     8941 aacgttctag catcatcaag attatggaga aaaacagtgg aagagacgat taagaatgaa
     9001 ttcccacaat tgactgttca acatcaattg attgattccg ctgctatgat cttggttaag
     9061 aacccaactc atctaaatgg tattattatc acaaacaata tgtttggtga tattatctcc
     9121 gatgaagcct ccgtgatccc aggttcgtta ggattacttc catctgcctc cttggcatct
     9181 cttccagata agaatacggc atttggttta tatgagccat gtcatggctc tgctccagac
     9241 ttaccaaagg gaaaggttaa ccccgtagca acaatcctat ctgctgctat gatgttgaag
     9301 ttgtctctgg acctgttcga agaaggtgaa attatagaac aagcagtcaa gaaagtgttg
     9361 gattctggta tcagaacagc ggatctaaag ggtaccaact ctactactga ggtcggtgat
     9421 gcagtagcga aagctgtcag ggaactatta gcttagactt agtagatgta cagttgcata
     9481 cataagtctg aacagaaaga agtaaatatc atatctagtt cggtttagaa atcagtgact
     9541 atattggttt tgaccagatc gcaatccttg caagcattta gtaatgacta tcaaaccggt
     9601 gaatcatctg ataaggctat attagcttgt gcattcgcat gtatcgggaa acgaacggta
     9661 ccgggcccgg caaccaaacc catacatcgg gattcctata ataccttcgt tggtctccct
     9721 aacatgtagg tggcggaggg gagatataca atagaacaga taccagacaa gacataatgg
     9781 gctaaacaag actacaccaa ttacactgcc tcattgatgg tggtacataa cgaactaata
     9841 ctgtagccct agacttgata gccatcatca tatcgaagtt tcactaccct ttttccattt
     9901 gccatctatt gaagtaataa taggcgcatg caacttcttt tctttttttt tcttttctct
     9961 ctcccccgtt gttgtctcac catatccgca atgacaaaaa aatgatggaa gacactaaag
    10021 gaaaaattta acgacaaaga cagcaccaac agatgtcgtt gttccagagc tgatgagggg
    10081 tatctcgaag cacacgaaac tttttccttc cttcattcac gcacactact ctctaatgag
    10141 caacggtata cggccttcct tccagttact tgaatttgaa ataaaaaaaa gtttgctgtc
    10201 ttgctatcaa gtataa
//
