LOCUS       sequence-180413-       11525 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpressing yeast farnesyl pyrophosphate synthase Erg20p mutant 
            N127W and Actinidia chinensis nerolidol/linalool synhtase AcNES1 
            under the control of TEF1/TEF2 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11525)
  AUTHORS   Peng B, Nielsen LK, Kampranis SC, Vickers CE
  TITLE     Engineered protein degradation of farnesyl pyrophosphate synthase is
            an effective regulatory mechanism to increase monoterpene production
            in Saccharomyces cerevisiae.
  JOURNAL   Metab Eng. 2018 Feb 19. pii: S1096-7176(17)30214-8. doi: 
            10.1016/j.ymben.2018.02.005.
  PUBMED    29471044
REFERENCE   2  (bases 1 to 11525)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab Eng. 
            2018 Feb 19. pii: S1096-7176(17)30214-8. doi: 
            10.1016/j.ymben.2018.02.005."
COMMENT     Sequence Label: pJT2
FEATURES             Location/Qualifiers
     source          1..11525
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        2277..2690
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     primer_bind     complement(4786..4806)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(4786..4804)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4825..4841)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4825..4841)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4838..4860)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4849..4865
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4873..4879,4880..4897,4898..4903))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     primer_bind     complement(5056..5073)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5227..5815)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5307..5326)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5986..6777,6778..6846))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6609..6628
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6847..6951)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      6978..8320
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(7941..7988)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     8361..8379
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8417..8439)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8539..8558
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     CDS             complement(9051..10145)
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKVDAVLLGAVAGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANLLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVDPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     promoter        complement(10158..10562)
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     rep_origin      complement(10862..11317)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10999..11020)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     11211..11230
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11447..11469
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     11461..11478
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     11462..11478
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11488..11507
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        11488..11506
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ctgcagactt tgttatgtag agttttttta gctacctata ttccaccata acatcaatca
       61 tgcggttgct ggtgtattta ccaataatgt ttaatgtata tatatatata tatatatggg
      121 gccgtatact tacatatagt agatgtcaag cgtaggcgct tcccctgccg gctgtgaggg
      181 cgccataacc aaggtatcta tagaccgcca atcagcaaac tacctccgta cattcatgtt
      241 gcacccacac atttatacac ccagaccgcg acaaattacc cataaggttg tttgtgacgg
      301 cgtcgtacaa gagaacgtgg gaacttttta ggctcaccaa aaaagaaaga aaaaatacga
      361 gttgctgaca gaagcctcaa gaaaaaaaaa attcttcttc gactatgctg gaggcagaga
      421 tgatcgagcc ggtagttaac tatatatagc taaattggtt ccatcacctt cttttctggt
      481 gtcgctcctt ctagtgctat ttctggcttt tcctattttt ttttttccat ttttctttct
      541 ctctttctaa tatataaatt ctcttgcatt ttctattttt ctctctatct attctacttg
      601 tttattccct tcaaggtttt tttttaagga gtacttgttt ttagaatata cggtcaacga
      661 actataatta actaaacatg gcttcagaaa aagaaattag gagagagaga ttcttgaacg
      721 ttttccctaa attagtagag gaattgaacg catcgctttt ggcttacggt atgcctaagg
      781 aagcatgtga ctggtatgcc cactcattga actacaacac tccaggcggt aagctaaata
      841 gaggtttgtc cgttgtggac acgtatgcta ttctctccaa caagaccgtt gaacaattgg
      901 ggcaagaaga atacgaaaag gttgccattc taggttggtg cattgagttg ttgcaggctt
      961 acttcttggt cgccgatgat atgatggaca agtccattac cagaagaggc caaccatgtt
     1021 ggtacaaggt tcctgaagtt ggggaaattg ccatcgggac gcattcatgt tagaggctgc
     1081 tatctacaag cttttgaaat ctcacttcag aaacgaaaaa tactacatag atatcaccga
     1141 attgttccat gaggtcacct tccaaaccga attgggccaa ttgatggact taatcactgc
     1201 acctgaagac aaagtcgact tgagtaagtt ctccctaaag aagcactcct tcatagttac
     1261 tttcaagact gcttactatt ctttctactt gcctgtcgca ttggccatgt acgttgccgg
     1321 tatcacggat gaaaaggatt tgaaacaagc cagagatgtc ttgattccat tgggtgaata
     1381 cttccaaatt caagatgact acttagactg cttcggtacc ccagaacaga tcggtaagat
     1441 cggtacagat atccaagata acaaatgttc ttgggtaatc aacaaggcat tggaacttgc
     1501 ttccgcagaa caaagaaaga ctttagacga aaattacggt aagaaggact cagtcgcaga
     1561 agccaaatgc aaaaagattt tcaatgactt gaaaattgaa cagctatacc acgaatatga
     1621 agagtctatt gccaaggatt tgaaggccaa aatttctcag gtcgatgagt ctcgtggctt
     1681 caaagctgat gtcttaactg cgttcttgaa caaagtttac aagagaagca aataatagtg
     1741 agaagttttg ttagaaaata aatcattttt taattgagca ttcttattcc tattttattt
     1801 aaatagtttt atgtattgtt agctacatac aacagtttaa atcaaatttt ctttttccca
     1861 agtccaaaat ggaggtttat tttgatgacc cgcatgcgat tatgttttga aagtataaga
     1921 ctacatacat gtacatatat ttaaacatgt aaacccgtcc attatattgc ttactttctt
     1981 cttttttgcc gttttgactt ggacctctgg tttgctattt ccttacaatc tttgctacaa
     2041 tggatccccc gggttgacag cctagacatc aatagtcata caacagaaag cgaccaccca
     2101 actttggctg ataatagcgt ataaacaatg catactttgt acgttcaaaa tacaatgcag
     2161 tagatatatt tatgcatatt acatataata catatcacat aggaagcaac aggcgcgttg
     2221 gacttttaat tttcgaggac cgcgaatcct tacatcacac ccaatccccc acaagtgatc
     2281 ccccacacac catagcttca aaatgtttct actccttttt tactcttcca gattttctcg
     2341 gactccgcgc atcgccgtac cacttcaaaa cacccaagca cagcatacta aatttcccct
     2401 ctttcttcct ctagggtgtc gttaattacc cgtactaaag gtttggaaaa gaaaaaagag
     2461 accgcctcgt ttctttttct tcgtcgaaaa aggcaataaa aatttttatc acgtttcttt
     2521 ttcttgaaaa tttttttttt tgattttttt ctctttcgat gacctcccat tgatatttaa
     2581 gttaataaac ggtcttcaat ttctcaagtt tcagtttcat ttttcttgtt ctattacaac
     2641 tttttttact tcttgctcat tagaaagaaa gcatagcaat ctaatctaag ttttaattac
     2701 aaaatggcta ccgcagcagg tcctatcgca actaacaact ccccacaaaa ctccaacgct
     2761 tacagaactc caatcgctcc ttccgtacca attactcata aatggtctat agctgaagat
     2821 ttgacatgta tttccaatcc tagtaagcac aataaccctc aaactggtta cagatcattt
     2881 tctgacgaat tatacgttaa gtacgaagaa aagttggaag atgttagaaa agcattaaga
     2941 gaagttgaag aaaacccttt ggaaggttta gttatgatag acgctttgca aagattgggt
     3001 atcgattacc atttcagagg tgaaattggt gcattcttgc aaaagcaaca aatcatatct
     3061 tcaactccag atggttaccc tgaacatggt ttgtacgaag tttcaacatt gtttagattc
     3121 ttaagacaag aaggtcacaa tgttaccgct gacgtcttta ataacttcaa ggataaggaa
     3181 ggtagattca gatcagaatt gtcaacagat attagaggtt tgatgtcctt atacgaagca
     3241 agtcaattga gaatagaagg tgaagacatc ttagatcaag ctgctgattt ctccagtcaa
     3301 ttgttaggta gatggacaaa agatcctaat catcacgaag ccagattggt ttctaacact
     3361 ttaacacatc cataccacaa gtcattggct acctttatgg gtcaaaaatt gtcctacatg
     3421 aactgcaagg gtccaaactg ggacggtgtc gataatttgc aagaattagc taagatggat
     3481 ttgactatcg tacaaagtat ccatcaaaag gaagtattcc aagtttctca atggtggaag
     3541 gatacaggtt tagccaatga attgaaattg gctagaaacc aaccattgaa gtggtatatg
     3601 tggcctatgg ccgctttaac cgatccaaga ttcagtgaag aaagagttga attaactaag
     3661 cctatttctt ttatatacat catagatgac atcttcgacg tctatggtac cattgaagaa
     3721 ttgaccttgt ttactgatgc agttaataga tgggaattgt ctgccgtcga acaattacca
     3781 gactacatga aagtatgttt caaggctttg tacgatgtta ccaacgaaat cgcatacaaa
     3841 atctataaaa agcatggtca aaaccctatt gattccttgc aaaagacttg ggctagtttg
     3901 tgcaatgcat ttttagttga agcaaagtgg ttcgcctctg gtcacttgcc aaatgcagaa
     3961 gaatacttaa agaacggtat catctcttca ggtgttcatg ttgtcttggc ccacatgttt
     4021 ttcttgttag gtgacggtat tacacaagaa tcagttgatt tggtagatga ctatccaggt
     4081 atttccacaa gtatcgcaac cattttgaga ttatctgatg acttgggttc agccaaagat
     4141 gaagaccaag atggttatga tggttcttac atcgaatgtt acatgaagga acataagggt
     4201 tccagtgtcg attcagccag agaagaagta ataagaatga tctccgaagc atggaaatgt
     4261 ttgaataagg aatgcttatc accaaaccct ttttctgaat cattcagaat aggttccttg
     4321 aatatggcta gaatgatccc tatgatgtac tcttacgatg acaaccataa cttgccaatt
     4381 ttagaagaac acatgaaggc aatgatctat aacacatctt tataatagtg agcggattga
     4441 gagcaaatcg ttaagttcag gtcaagtaaa aattgatttc gaaaactaat ttctcttata
     4501 caatcctttg attggaccgt catcctttcg aatataagat tttgttaaga atattttaga
     4561 cagagatcta ctttatattt aatatctaga tattacataa tttcctctct aataaaatat
     4621 cattaataaa ataaaaatga agcgatttga ttttgtgttg tcaacttagt ttgccgctat
     4681 gcctcttggg taatgctatt attgaatcga agggctttat tatattaccc tttagcttat
     4741 tctgaggttt ctgtggcgtc cgcggtggag ctccagcttt tgttcccttt agtgagggtt
     4801 aattgcgcgc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     4861 cacaattcca cacaacatag gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     4921 agtgaggtaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     4981 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     5041 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     5101 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     5161 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     5221 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     5281 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     5341 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     5401 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     5461 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     5521 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     5581 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     5641 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc
     5701 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     5761 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     5821 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     5881 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     5941 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     6001 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     6061 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     6121 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     6181 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     6241 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     6301 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     6361 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     6421 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     6481 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     6541 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     6601 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     6661 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     6721 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     6781 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     6841 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     6901 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     6961 ccgaaaagtg ccacctgaac gaagcatctg tgcttcattt tgtagaacaa aaatgcaacg
     7021 cgagagcgct aatttttcaa acaaagaatc tgagctgcat ttttacagaa cagaaatgca
     7081 acgcgaaagc gctattttac caacgaagaa tctgtgcttc atttttgtaa aacaaaaatg
     7141 caacgcgaga gcgctaattt ttcaaacaaa gaatctgagc tgcattttta cagaacagaa
     7201 atgcaacgcg agagcgctat tttaccaaca aagaatctat acttcttttt tgttctacaa
     7261 aaatgcatcc cgagagcgct atttttctaa caaagcatct tagattactt tttttctcct
     7321 ttgtgcgctc tataatgcag tctcttgata actttttgca ctgtaggtcc gttaaggtta
     7381 gaagaaggct actttggtgt ctattttctc ttccataaaa aaagcctgac tccacttccc
     7441 gcgtttactg attactagcg aagctgcggg tgcatttttt caagataaag gcatccccga
     7501 ttatattcta taccgatgtg gattgcgcat actttgtgaa cagaaagtga tagcgttgat
     7561 gattcttcat tggtcagaaa attatgaacg gtttcttcta ttttgtctct atatactacg
     7621 tataggaaat gtttacattt tcgtattgtt ttcgattcac tctatgaata gttcttacta
     7681 caattttttt gtctaaagag taatactaga gataaacata aaaaatgtag aggtcgagtt
     7741 tagatgcaag ttcaaggagc gaaaggtgga tgggtaggtt atatagggat atagcacaga
     7801 gatatatagc aaagagatac ttttgagcaa tgtttgtgga agcggtattc gcaatatttt
     7861 agtagctcgt tacagtccgg tgcgtttttg gttttttgaa agtgcgtctt cagagcgctt
     7921 ttggttttca aaagcgctct gaagttccta tactttctag agaataggaa cttcggaata
     7981 ggaacttcaa agcgtttccg aaaacgagcg cttccgaaaa tgcaacgcga gctgcgcaca
     8041 tacagctcac tgttcacgtc gcacctatat ctgcgtgttg cctgtatata tatatacatg
     8101 agaagaacgg catagtgcgt gtttatgctt aaatgcgtac ttatatgcgt ctatttatgt
     8161 aggatgaaag gtagtctagt acctcctgtg atattatccc attccatgcg gggtatcgta
     8221 tgcttccttc agcactaccc tttagctgtt ctatatgctg ccactcctca attggattag
     8281 tctcatcctt caatgctatc atttcctttg atattggatc atactaagaa accattatta
     8341 tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg
     8401 gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt
     8461 aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc
     8521 ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac catatcgact
     8581 acgtcgtaag gccgtttctg acagagtaaa attcttgagg gaactttcac cattatggga
     8641 aatgcttcaa gaaggtattg acttaaactc catcaaatgg tcaggtcatt gagtgttttt
     8701 tatttgttgt attttttttt ttttagagaa aatcctccaa tatcaaatta ggaatcgtag
     8761 tttcatgatt ttctgttaca cctaactttt tgtgtggtgc cctcctcctt gtcaatatta
     8821 atgttaaagt gcaattcttt ttccttatca cgttgagcca ttagtatcaa tttgcttacc
     8881 tgtattcctt tactatcctc ctttttctcc ttcttgataa atgtatgtag attgcgtata
     8941 tagtttcgtc taccctatga acatattcca ttttgtaatt tcgtgtcgtt tctattatga
     9001 atttcattta taaagtttat gtacaaatat cataaaaaaa gagaatcttt ttaagcaagg
     9061 attttcttaa cttcttcggc gacagcatca ccgacttcgg tggtactgtt ggaaccacct
     9121 aaatcaccag ttctgatacc tgcatccaaa acctttttaa ctgcatcttc aatggcctta
     9181 ccttcttcag gcaagttcaa tgacaatttc aacatcattg cagcagacaa gatagtggcg
     9241 atagggtcaa ccttattctt tggcaaatct ggagcagaac cgtggcatgg ttcgtacaaa
     9301 ccaaatgcgg tgttcttgtc tggcaaagag gccaaggacg cagatggcaa caaacccaag
     9361 gaacctggga taacggaggc ttcatcggag atgatatcac caaacatgtt gctggtgatt
     9421 ataataccat ttaggtgggt tgggttctta actaggatca tggcggcaga atcaatcaat
     9481 tgatgttgaa ccttcaatgt agggaattcg ttcttgatgg tttcctccac agtttttctc
     9541 cataatcttg aagaggccaa aagattagct ttatccaagg accaaatagg caatggtggc
     9601 tcatgttgta gggccatgaa agcggccatt cttgtgattc tttgcacttc tggaacggtg
     9661 tattgttcac tatcccaagc gacaccatca ccatcgtctt cctttctctt accaaagtaa
     9721 atacctccca ctaattctct gacaacaacg aagtcagtac ctttagcaaa ttgtggcttg
     9781 attggagata agtctaaaag agagtcggat gcaaagttac atggtcttaa gttggcgtac
     9841 aattgaagtt ctttacggat ttttagtaaa ccttgttcag gtctaacact accggtaccc
     9901 catttaggac cagccacagc acctaacaaa acggcatcaa ccttcttgga ggcttccagc
     9961 gcctcatctg gaagtgggac acctgtagca tcgatagcag caccaccaat taaatgattt
    10021 tcgaaatcga acttgacatt ggaacgaaca tcagaaatag ctttaagaac cttaatggct
    10081 tcggctgtga tttcttgacc aacgtggtca cctggcaaaa cgacgatctt cttaggggca
    10141 gacatagggg cagacattag aatggtatat ccttgaaata tatatatata ttgctgaaat
    10201 gtaaaaggta agaaaagtta gaaagtaaga cgattgctaa ccacctattg gaaaaaacaa
    10261 taggtcctta aataatattg tcaacttcaa gtattgtgat gcaagcattt agtcatgaac
    10321 gcttctctat tctatatgaa aagccggttc cggcctctca cctttccttt ttctcccaat
    10381 ttttcagttg aaaaaggtat atgcgtcagg cgacctctga aattaacaaa aaatttccag
    10441 tcatcgaatt tgattctgtg cgatagcgcc cctgtgtgtt ctcgttatgt tgaggaaaaa
    10501 aataatggtt gctaagagat tcgaactctt gcatcttacg atacctgagt attcccacag
    10561 ttaactgcgg tcaagatatt tcttgaatca ggcgccttag accgctcggc caaacaacca
    10621 attacttgtt gagaaataga gtataattat cctataaata taacgttttt gaacacacat
    10681 gaacaaggaa gtacaggaca attgattttg aagagaatgt ggattttgat gtaattgttg
    10741 ggattccatt tttaataagg caataatatt aggtatgtgg atatactaga agttctcctc
    10801 gaccgtcgat atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag
    10861 gaaattgtaa acgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca
    10921 ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag
    10981 atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc
    11041 aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc
    11101 taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc
    11161 ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa
    11221 gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc
    11281 acacccgccg cgcttaatgc gccgctacag ggcgcgtcgc gccattcgcc attcaggctg
    11341 cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa
    11401 gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt
    11461 tgtaaaacga cggccagtga gcgcgcgtaa tacgactcac tatagggcga attgggtacc
    11521 gggcc
//
