LOCUS       sequence-180423-       14530 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Enhancing the upper-part mevalonate pathway; overexpressing 
            codon-optimized Enterococcus faecalis mevalonate pathway genes under
            the control of galactose-inducible promoters; integrated into genome
            HMG2 locus; overexpressing HMG2 K6R..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14530)
  AUTHORS   Peng B, Plan MR, Carpenter A, Nielsen LK, Vickers CE
  TITLE     Coupling gene regulatory patterns to bioprocess conditions to 
            optimize synthetic metabolic modules for improved sesquiterpene 
            production in yeast.
  JOURNAL   Biotechnol Biofuels. 2017 Feb 21;10:43. doi: 
            10.1186/s13068-017-0728-x.
  PUBMED    28239415
REFERENCE   2  (bases 1 to 14530)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Biofuels."; date: "2017-02-21"; volume: "10:43. doi"; pages: " 
            10.1186/s13068-017-0728-x"
COMMENT     Sequence Label: pIMVAu1
FEATURES             Location/Qualifiers
     source          1..14530
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(26..614)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(106..125)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(785..1576,1577..1645))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1408..1427
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1646..1750)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      1777..3119
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(2740..2787)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     3159..3177
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(3215..3237)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3337..3356
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        3503..3689
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
     CDS             complement(4569..5231)
                     /codon_start=1
                     /gene="S. cerevisiae HIS3"
                     /product="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /label=HIS3
                     /note="yeast auxotrophic marker"
                     /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE
                     QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF
                     KEALGAVRGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHF
                     LESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM"
     promoter        complement(5232..5419)
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
     promoter        complement(6903..7344)
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     primer_bind     complement(6909..6932)
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     protein_bind    7227..7344
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
ORIGIN
        1 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
       61 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
      121 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
      181 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
      241 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
      301 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
      361 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
      421 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
      481 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
      541 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
      601 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
      661 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
      721 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
      781 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
      841 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
      901 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
      961 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     1021 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     1081 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     1141 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     1201 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     1261 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     1321 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     1381 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     1441 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     1501 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     1561 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     1621 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     1681 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     1741 gggttccgcg cacatttccc cgaaaagtgc cacctgaacg aagcatctgt gcttcatttt
     1801 gtagaacaaa aatgcaacgc gagagcgcta atttttcaaa caaagaatct gagctgcatt
     1861 tttacagaac agaaatgcaa cgcgaaagcg ctattttacc aacgaagaat ctgtgcttca
     1921 tttttgtaaa acaaaaatgc aacgcgagag cgctaatttt tcaaacaaag aatctgagct
     1981 gcatttttac agaacagaaa tgcaacgcga gagcgctatt ttaccaacaa agaatctata
     2041 cttctttttt gttctacaaa aatgcatccc gagagcgcta tttttctaac aaagcatctt
     2101 agattacttt ttttctcctt tgtgcgctct ataatgcagt ctcttgataa ctttttgcac
     2161 tgtaggtccg ttaaggttag aagaaggcta ctttggtgtc tattttctct tccataaaaa
     2221 aagcctgact ccacttcccg cgtttactga ttactagcga agctgcgggt gcattttttc
     2281 aagataaagg catccccgat tatattctat accgatgtgg attgcgcata ctttgtgaac
     2341 agaaagtgat agcgttgatg attcttcatt ggtcagaaaa ttatgaacgg tttcttctat
     2401 tttgtctcta tatactacgt ataggaaatg tttacatttt cgtattgttt tcgattcact
     2461 ctatgaatag ttcttactac aatttttttg tctaaagagt aatactagag ataaacataa
     2521 aaaatgtaga ggtcgagttt agatgcaagt tcaaggagcg aaaggtggat gggtaggtta
     2581 tatagggata tagcacagag atatatagca aagagatact tttgagcaat gtttgtggaa
     2641 gcggtattcg caatatttta gtagctcgtt acagtccggt gcgtttttgg ttttttgaaa
     2701 gtgcgtcttc agagcgcttt tggttttcaa aagcgctctg aagttcctat actttctaga
     2761 gaataggaac ttcggaatag gaacttcaaa gcgtttccga aaacgagcgc ttccgaaaat
     2821 gcaacgcgag ctgcgcacat acagctcact gttcacgtcg cacctatatc tgcgtgttgc
     2881 ctgtatatat atatacatga gaagaacggc atagtgcgtg tttatgctta aatgcgtact
     2941 tatatgcgtc tatttatgta ggatgaaagg tagtctagta cctcctgtga tattatccca
     3001 ttccatgcgg ggtatcgtat gcttccttca gcactaccct ttagctgttc tatatgctgc
     3061 cactcctcaa ttggattagt ctcatccttc aatgctatca tttcctttga tattggatca
     3121 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct
     3181 ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga
     3241 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag
     3301 cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga
     3361 gagtgcacca taaattcccg ttttaagagc ttggtgagcg ctaggagtca ctgccaggta
     3421 tcgtttgaac acggcattag tcagggaagt cataacacag tcctttcccg caattttctt
     3481 tttctattac tcttggcctc ctctagtaca ctctatattt ttttatgcct cggtaatgat
     3541 tttcattttt ttttttcccc tagcggatga ctcttttttt ttcttagcga ttggcattat
     3601 cacataatga attatacatt atataaagta atgtgatttc ttcgaagaat atactaaaaa
     3661 atgagcaggc aagataaacg aaggcaaaga tgacagagca gaaagcccta gtaaagcgta
     3721 ttacaaatga aaccaagatt cagattgcga tctctttaaa gggtggtccc ctagcgatag
     3781 agcactcgat cttcccagaa aaagaggcag aagcagtagc agaacaggcc aattttgtag
     3841 aacaaacaca atcgcaagtg attaacgtcc acacaggtat agggtttctg gaccatatga
     3901 tacatgctct ggccaagcat tccggctggt cgctaatcgt tgagtgcatt ggtgacttac
     3961 acatagacga ccatcacacc actgaagact gcgggattgc tctcggtcaa gcttttaaag
     4021 aggccctact ggcgcgtgga gtaaaaaggt ttggatcagg atttgcgcct ttggatgagg
     4081 cactttccag agcggtggta gatctttcga acaggccgta cgcagttgtc gaacttggtt
     4141 tgcaaaggga gaaagtagga gatctctctt gcgagatgat cccgcatttt cttgaaagct
     4201 ttgcagaggc tagcttttaa ccaataggcc gaaatcggca aaatccctta tttaaataga
     4261 ccattgatcg ttagagacga gcgcattgtt ttgttgaatc cctttcaaca gcgcgacaaa
     4321 agttaatcga gaacaataag atgttgcatt caagggttag tcaaacatat cagaaggccg
     4381 ttttctaaat cacctcaaga acctaccaca gaaagcgcgc ctcgttcaga atgacacgta
     4441 tagaatgatg cattaccttg tcatcttcag tatcatactg ttcgtataca tacttactga
     4501 cattcatagg tatacatata tacacatgta tatatatcgt atgctgcagc tttaaataat
     4561 cggtgtcact acataagaac acctttggtg gagggaacat cgttggtacc attgggcgag
     4621 gtggcttctc ttatggcaac cgcaagagcc ttgaacgcac tctcactacg gtgatgatca
     4681 ttcttgcctc gcagacaatc aacgtggagg gtaattctgc tagcctctgc aaagctttca
     4741 agaaaatgcg ggatcatctc gcaagagaga tctcctactt tctccctttg caaaccaagt
     4801 tcgacaactg cgtacggcct gttcgaaaga tctaccaccg ctctggaaag tgcctcatcc
     4861 aaaggcgcaa atcctgatcc aaaccttttt actccacgca cggcccctag ggcctcttta
     4921 aaagcttgac cgagagcaat cccgcagtct tcagtggtgt gatggtcgtc tatgtgtaag
     4981 tcaccaatgc actcaacgat tagcgaccag ccggaatgct tggccagagc atgtatcata
     5041 tggtccagaa accctatacc tgtgtggacg ttaatcactt gcgattgtgt ggcctgttct
     5101 gctactgctt ctgcctcttt ttctgggaag atcgagtgct ctatcgctag gggaccaccc
     5161 tttaaagaga tcgcaatctg aatcttggtt tcatttgtaa tacgctttac tagggctttc
     5221 tgctctgtca tctttgcctt cgtttatctt gcctgctcat tttttagtat attcttcgaa
     5281 gaaatcacat tactttatat aatgtataat tcattatgtg ataatgccaa tcgctaagaa
     5341 aaaaaaagag tcatccgcta ggtggaaaaa aaaaaatgaa aatcattacc gaggcataaa
     5401 aaaatataga gtgtactagg atcctactga aattcatgac acaaataacg acatattttg
     5461 ttaaagattt gtcggaggta gaacttcatg ggcacatgca ttgtattgat aaaaaaatgc
     5521 tggacaagtc gaaattcatg tatttatacg aagagaaaag caacgaggaa gtgaaagcag
     5581 cttctcctca aaaggagaag atagtagata atatcattgg cctattatca aatgatgaag
     5641 aacattaaca tgaattggaa aaagaaaaaa ttgcatgtaa atatatacat acgtagaatt
     5701 ttatacttag gctacagatc aaagctatca ttaatttctg tatgatctaa ctgtgttatt
     5761 gatagcactg atggagtact tcaattcatc ttctaaagtt tgatcgatgt cagtatccaa
     5821 ggtttcagca aacatggctt cgtattctgc aatagataat tcggttctgt tatctaacaa
     5881 agccaagtgt gtttcctttt gcaaatggtt ttggtaacca gcgaccaatt caccagtgaa
     5941 aaattcggca actgcacctg aaccataaga aaacaaaccg atttggttac ctgcggtcaa
     6001 tgtagtagcg ttttctaaca aacttatcaa acctaagtac aaggaacctg tatacaaatt
     6061 accaactctt ctactgtata tgatggattc ttcgtatctg gctaatattc tttcttgttc
     6121 tgcttcggtt tgatctgaaa tctttgctaa caaagccttt ttacccattt ttgtgtatgg
     6181 aatgtggaaa gccaaggcgt cataatctgc gaaatctaaa ccagttctct ttttatgttc
     6241 gtcccaaact tgagcgaatg attgtatgta ggtttcgtta gacaaaggac catcaaccat
     6301 agggtatgga tgacctgttg gtctccagaa atcatagatg tcttgagtca acataacgtt
     6361 gtcttccttc aaagccaaga ttctaggttc tgaggcgacc aacattgcaa cagcaccggc
     6421 accttgagtt ggttcaccac cagaatttaa accgtattta gcaatatcgg ctgcaactac
     6481 caataccttt ttgtcagggt gtaaggcgac atgattcttt gctaattgca aacctgctgt
     6541 agcaccataa catgcttctt tgatttcgaa acttctagca aatggttgta tacccatcaa
     6601 tctgtgtaag acaacagcgg ctgctttgga ttcatcaatt gaagattcag tacctacaat
     6661 gaccatatcg attgcttcct tgtcttcttt agttaaaatg gcttcagcgg cgtttgcagc
     6721 gaaggtaact atatcttggg aaataggatt tacagccatt tggtcttgac caataccgat
     6781 atgaaattta ccagggtcta cgtttctggc ttctgctaat gctgtcatgt caatgtaata
     6841 aggagggacg aaaaaggaga ttttgtcaat accgatggtc attttgaatt ctatagtttt
     6901 ttctccttga cgttaaagta tagaggtata ttaacaattt tttgttgata cttttatgac
     6961 atttgaataa gaagtaatac aaactgaaaa tgttgaaagt attagttaaa gtggttatgc
     7021 agcttttcca tttatatatc tgttaataga tcaaaaatca tcgcttcgct gattaattac
     7081 cccagaaata aggctaaaaa actaatcgca ttatcatcct atggttgtta atttgattcg
     7141 ttaatttgaa ggtttgtggg gccaggttac tgccaatttt tcctcttcat aaccataaaa
     7201 gctagtattg tagaatcttt attgttcgga gcagtgcggc gcgaggcaca tctgcgtttc
     7261 aggaacgcga ccggtgaaga cgaggacgca cggaggagag tcttccgtcg gagggctgtc
     7321 gcccgctcgg cggcttctaa tccgtacttc aatatagcaa tgagcagtta agcgtattac
     7381 tgaaagttcc aaagagaagg tttttttagg ctaagataat ggggctcttt acatttccac
     7441 aacatataag taagattaga tatggatatg tatatggtgg taatgccatg taatatgatt
     7501 attaaacttc tttgcgtcca tccaaaaaaa aagtaagaat ttttgaaaat tcaatataag
     7561 tcgacaaaat gacaatcaag gaacataaag tagtttatga agctcacaac gtaaaggctc
     7621 ttaaggctcc tcaacatttt tacaacagcc aacccggcaa gggttacgtt actgatatgc
     7681 aacattatca agaaatgtat caacaatcta tcaatgagcc agaaaaattc tttgataaga
     7741 tggctaagga atacttgcat tgggatgctc catacaccaa agttcaatct ggttcattga
     7801 acaatggtga tgttgcatgg tttttgaacg gtaaattgaa tgcatcatac aattgtgttg
     7861 acagacatgc ctttgctaat cccgacaagc cagctttgat ctatgaagct gatgacgaat
     7921 ccgacaacaa aatcatcaca tttggtgaat tactcagaaa agtttcccaa atcgctggtg
     7981 tcttaaaaag ctggggcgtt aagaaaggtg acacagtggc tatctatttg ccaatgattc
     8041 cagaagcggt cattgctatg ttggctgtgg ctcgtattgg tgctattcac tctgttgtct
     8101 ttgctgggtt ctccgctggt tcgttgaaag atcgtgtcgt tgacgctaat tctaaagtgg
     8161 tcatcacttg tgatgaaggt aaaagaggtg gtaagaccat caacactaaa aaaattgttg
     8221 acgaaggttt gaacggagtc gatttggttt cccgtatctt ggttttccaa agaactggta
     8281 ctgaaggtat tccaatgaag gccggtagag attactggtg gcatgaggag gccgctaagc
     8341 agagaactta cctacctcct gtttcatgtg acgctgaaga tcctctattt ttattataca
     8401 cttccggttc cactggttct ccaaagggtg tcgttcacac tacaggtggt tatttattag
     8461 gtgccgcttt aacaactaga tacgtttttg atattcaccc agaagatgtt ctcttcactg
     8521 ccggtgacgt cggctggatc acgggtcaca cctatgctct atatggtcca ttaaccttgg
     8581 gtaccgcctc aataattttc gaatccactc ctgcctaccc agattatggt agatattgga
     8641 gaattatcca acgtcacaag gctacccatt tctatgtggc tccaactgct ttaagattaa
     8701 tcaaacgtgt aggtgaagcc gaaattgcca aatatgacac ttcctcatta cgtgtcttgg
     8761 gttccgtcgg tgaaccaatc tctccagact tatgggaatg gtatcatgaa aaagtgggta
     8821 acaaaaactg tgtcatttgt gacactatgt ggcaaacaga gtctggttct catttaattg
     8881 ctcctttggc aggtgctgtc ccaacaaaac ctggttctgc taccgtgcca ttctttggta
     8941 ttaacgcttg tatcattgac cctgttacag gtgtggaatt agaaggtaat gatgtcgaag
     9001 gtgtccttgc cgttaaatca ccatggccat caatggctag atctgtttgg aaccaccacg
     9061 accgttacat ggatacttac ttgaaacctt atcctggtca ctatttcaca ggtgatggtg
     9121 ctggtagaga tcatgatggt tactactgga tcaggggtag agttgacgac gttgtaaatg
     9181 tttccggtca tagattatcc acatcagaaa ttgaagcatc tatctcaaat cacgaaaacg
     9241 tctcggaagc tgctgttgtc ggtattccag atgaattgac cggtcaaacc gtcgttgcat
     9301 atgtttccct aaaagatggt tatctacaaa acaacgctac tgaaggtgat gcagaacaca
     9361 tcacaccaga taatttacgt agagaattga tcttacaagt taggggtgag attggtcctt
     9421 tcgcctcacc aaaaaccatt attctagtta gagatctacc aagaacaagg tcaggaaaga
     9481 ttatgagaag agttctaaga aaggttgctt ctaacgaagc cgaacagcta ggtgacctaa
     9541 ctactttggc caacccagaa gttgtacctg ccatcatttc tgctgtagag aaccaatttt
     9601 tctctcaaaa aaagaaataa cttaaatgag aaaaatttcg taatgagata aaatttcgct
     9661 ccttttctgt tttctatttt ctattttccc aacttttgct ctattcagtt ataaattact
     9721 atttatccat cagttaaaaa acaagatctt ttactggtca gctaggaaag cgaaaataca
     9781 aagactttat gcacttagtg atatatatgt atagatatat ccatttttac gcacttatca
     9841 tatatcttag ttatctaaat acaatctagt tattcgtaca caatcgcccc tgttatccct
     9901 atagtgggaa taaagtaatg catgctgtac taatccaagg aggtttacgg accaggggaa
     9961 ctttccagat tcagatcaca gcaatatagg actagaaaat atcaggtagc cgcactcaac
    10021 ttgtaactgg caactacttt gcatcaaact ccaattaaat gcggtagaat cttttcacaa
    10081 aaggtactca acgtcaattc ggaaagcttc cttccggaat ggcttaagta ggttgcaatt
    10141 tctttttcta ttagtagcta aaaatgggtc acgtgatcta tattcgaaag gggcggttgc
    10201 ctcaggaagg caccggcggt ctttcgtccg tgcggagata tctgcgccgt tcaggggtcc
    10261 atgtgccttg gacgatatta aggcagaagg cagtatcggg gcggatcact ccgaaccgag
    10321 attagttaag cccttcccat ctcaagatgg ggagcaaatg gcattatact cctgctagaa
    10381 agttaactgt gcacatattc ttaaattata caacattctg gagagctatt gttcaaaaaa
    10441 caaacatttc gcaggctaaa atgtggagat aggataagtt ttgtagacat atataaacaa
    10501 tcagtaattg gattgaaaat ttggtgttgt gaattgctct tcattatgca ccttattcaa
    10561 ttatcatcaa gaatagtaat agttaagtaa acacaagatt aacataataa aaaaaataat
    10621 tctttcataa ctagtaaaat gaagactgta gtaataatag acgcattgag aacaccaatc
    10681 ggtaaatata aaggtagttt gtcccaagtt tccgctgtag acttaggtac tcatgttact
    10741 acacaattgt tgaagagaca ctccacaatc agtgaagaaa tcgatcaagt catattcggt
    10801 aacgtattac aagctggtaa cggtcaaaac ccagctagac aaatagcaat caattctggt
    10861 ttgtcacatg aaattcctgc tatgacagta aacgaagttt gtggttcagg catgaaagcc
    10921 gtaattttgg ctaagcaatt gatccaattg ggtgaagccg aagttttgat cgctggtggt
    10981 atagaaaaca tgagtcaagc tccaaaatta caaagattca attacgaaac tgaatcttac
    11041 gatgcacctt tctcttcaat gatgtatgat ggtttaactg acgcattttc tggtcaagcc
    11101 atgggtttga cagcagaaaa tgtagccgaa aagtaccatg ttaccagaga agaacaagat
    11161 caattttccg ttcacagtca attgaaagct gcacaagcac aagccgaagg tattttcgct
    11221 gacgaaatag caccattaga agtttctggt acattggtcg aaaaggatga aggtataaga
    11281 cctaactcca gtgttgaaaa attgggtact ttgaagacag ttttcaagga agacggtaca
    11341 gtcaccgccg gtaatgcttc taccattaac gatggtgcta gtgcattgat aatcgcttct
    11401 caagaatatg ccgaagctca tggtttgcca tacttagcta tcattagaga tagtgtagaa
    11461 gttggtattg acccagcata tatgggtatt tctcctataa aagccatcca aaagttgttg
    11521 gctagaaacc aattgaccac tgaagaaatc gatttgtacg aaattaacga agcatttgcc
    11581 gctacttcaa tcgttgtcca aagagaattg gcattgcctg aagaaaaggt taacatctat
    11641 ggtggtggta tctccttagg tcacgcaatt ggtgccaccg gtgctagatt gttgacttcc
    11701 ttgagttacc aattgaacca aaaggaaaag aaatacggtg ttgcttcttt gtgcattggt
    11761 ggtggtttgg gtttagcaat gttgttagaa agaccacaac aaaagaaaaa ttcaagattc
    11821 taccaaatgt cccctgaaga aagattggca tcattgttga acgaaggtca aatttccgcc
    11881 gatactaaga aagaatttga aaacaccgca ttgtcttcac aaatcgccaa ccatatgatc
    11941 gaaaaccaaa tctctgaaac agaagttcca atgggtgtcg gtttgcactt aactgtagat
    12001 gaaacagact atttggttcc aatggctacc gaagaaccta gtgttatcgc agccttgtct
    12061 aatggtgcaa aaattgccca aggttttaag actgttaacc aacaaagatt gatgagaggt
    12121 caaattgtat tctacgatgt tgcagacgcc gaatcattga tcgatgaatt acaagttaga
    12181 gaaactgaaa tctttcaaca agctgaatta tcttacccat caatcgttaa gagaggtggt
    12241 ggtttgagag atttgcaata cagagctttt gacgaatcct tcgtcagtgt agatttcttg
    12301 gttgatgtca aagacgctat gggtgcaaat attgtcaacg ctatgttaga aggtgtagca
    12361 gaattgttta gagaatggtt cgctgaacaa aagattttgt tttctatctt gtcaaactat
    12421 gctacagaat ctgtagttac catgaaaact gcaattccag tttccagatt atctaagggt
    12481 tctaacggta gagaaatcgc tgaaaagatt gtcttagcct caagatatgc ttccttggac
    12541 ccttacagag cagttactca taataagggt ataatgaacg gtatcgaagc agtcgtattg
    12601 gccaccggta atgatactag agccgtttct gcttcatgtc acgctttcgc agtcaaggaa
    12661 ggtagatatc aaggtttgac atcatggacc ttagatggtg aacaattgat tggtgaaata
    12721 tccgttccat tggccttagc tactgtcggt ggtgctacaa aagtattacc taagagtcaa
    12781 gctgctgctg atttgttagc tgtaacagac gcaaaagaat tgtcaagagt tgtcgctgct
    12841 gttggtttgg cccaaaattt ggccgcttta agagctttgg tctcagaagg tattcaaaag
    12901 ggtcatatgg cattacaagc cagatccttg gctatgacag ttggtgcaac cggtaaagaa
    12961 gtcgaagcag tagcccaaca attgaagaga caaaagacta tgaaccaaga cagagcttta
    13021 gcaattttga acgatttgag aaaacaataa tgatagtatt gttcgttttt tatcatagtt
    13081 tttcaaatct tcatatgaat actaggtaga aatttggctt atcagtacga atgaattaca
    13141 gtttgactga tttgtacacg agaattgacg tataacttct tttgaaagtc tttgaaatta
    13201 catggtagta catacatata tatatatata tatatatgtt atattattgt taattaaata
    13261 ttattatgcg tattttcctt ttctttatta gtatagtatt aatgacagta ataataataa
    13321 taatagtaac aatatctctt tttttttttc agtgagcttt tatttttttt tcattgctct
    13381 tcttttggcc tcttttgttt ttttcttgat ttcctccagt ttcatctgtt ttttctttgg
    13441 atcagataca aaatctggtt tgaacgcgag ctctttgcca gcttactatc cttcttgaaa
    13501 atatgcactc tatatctttt agttcttaat tgcaacacat agatttgctg tataacgaat
    13561 tttatgctat tttttaaatt tggagttcag tgataaaagt gtcacagcga atttcctcac
    13621 atgtagggac cgaattgttt acaagttctc tgtaccacca tggagacatc aaaaattgaa
    13681 aatctatgga aagatatgga cggtagcaac aagaatatag cacgagccgc ggagttcatt
    13741 tcgttacttt tgatatcact cacaactatt gcgaagcgct tcagtgaaaa aatcataagg
    13801 aaaagttgta aatattattg gtagtattcg tttggtaaag tagagggggt aatttttccc
    13861 ctttattttg ttcatacatt cttaaattgc tttgcctctc cttttggaaa gctatacttc
    13921 ggagcactgt tgagcgaagg ctcattagat atattttctg tcattttcct taacccaaaa
    13981 ataagggaaa gggtccaaaa agcgctcgga caactgttga ccgtgatccg aaggactggc
    14041 tatacagtgt tcacaaaata gccaagctga aaataatgtg tagctatgtt cagttagttt
    14101 ggctagcaaa gatataaaag caggtcggaa atatttatgg gcattattat gcagagcatc
    14161 aacatgataa aaaaaaacag ttgaatattc cctcaaaaat gtcacttccc ttaagaacga
    14221 tagtacattt ggtaaagccc tttgcttgca ctgctaggtt tagtgcgaga tacccaatcc
    14281 acgtcattgt tgttgctgtt ttattgagtg ccgctgctta tctatccgtg acacaatctt
    14341 accttaacga atggaagctg gactctaatc agtattctac atacttaagc ataaagccgg
    14401 atgagttgtt tgaaaaatgc acacactact ataggtctcc tgtgtctgat acatggaagt
    14461 tactcagctc taaagaagcc gccgatattt aaatacatgt gagcaaaagg ccagcaaaag
    14521 gccaggaacc
//
