LOCUS       sequence-180434-         740 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses His-tagged mouse ECHB (35-475) in E.coli cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 740)
  AUTHORS   Sadhukhan S, Liu X, Ryu D, Nelson OD, Stupinski JA, Li Z, Chen W, 
            Zhang S, Weiss RS, Locasale JW, Auwerx J, Lin H
  TITLE     Metabolomics-assisted proteomics identifies succinylation and SIRT5 
            as important regulators of cardiac function.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 Apr 19;113(16):4320-5. doi: 
            10.1073/pnas.1519858113. Epub 2016 Apr 5.
  PUBMED    27051063
REFERENCE   2  (bases 1 to 740)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1519858113"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2016-04-19- 19"; volume: "113"; issue: "16"; pages: "4320-5"
COMMENT     Sequence Label: pet-ECHB(35-475)
FEATURES             Location/Qualifiers
     source          1..740
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             22..44
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             63..80
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             90..107
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             111..143
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
ORIGIN
        1 gngnaattcc ctctgaataa ttttgtttaa ctttaagaag gagatatacc atgggcagca
       61 gccatcatca tcatcatcac agcagcggcc tggtgccgcg cggcagccat atggctagca
      121 tgactggtgg acagcaaatg ggtcgcggat ccgaattctc tgccccagct gtccagacca
      181 agtcaaagaa gactttagca aaacccaata tgaagaatat tgtggtggtg gaaggggtcc
      241 gcattccatt tctgctgtca ggcacttcgt ataaagacct aatgccacat gacttggcta
      301 gagctgcact ttcgggtttg ttgcatcgga ccaatattcc aaaggatgtt gttgattata
      361 tcatctttgg tacagttatt caggaagtga aaacaagcaa tgtggctaga gaggctgccc
      421 tgggagctgg cttctctgat aagactccag ctcacactgt caccatggct tgtatctctt
      481 caaaccaagc catgaccaca gctgttggtc tgatagcttc tggccagtgt gatgtcgtcg
      541 tggctggtgg tgttgagtta atgtctgatg tccctattcg tcattcaaga aatatgagga
      601 aaatgatgct tgatctcaat aaagccaaga ctctgggcca gcgcctgtcc ttactcagta
      661 aattcagatt gaattttctg tcccctgagc tccctgcagt ggctgagttc tccactaatg
      721 agaccatggg ccactctgca
//
