LOCUS       sequence-180435-         768 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses His-tagged mouse ECHB (35-475) in E.coli cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 768)
  AUTHORS   Sadhukhan S, Liu X, Ryu D, Nelson OD, Stupinski JA, Li Z, Chen W, 
            Zhang S, Weiss RS, Locasale JW, Auwerx J, Lin H
  TITLE     Metabolomics-assisted proteomics identifies succinylation and SIRT5 
            as important regulators of cardiac function.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 Apr 19;113(16):4320-5. doi: 
            10.1073/pnas.1519858113. Epub 2016 Apr 5.
  PUBMED    27051063
REFERENCE   2  (bases 1 to 768)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1519858113"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2016-04-19- 19"; volume: "113"; issue: "16"; pages: "4320-5"
COMMENT     Sequence Label: pet-ECHB(35-475)
FEATURES             Location/Qualifiers
     source          1..768
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(31..48)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 actcttcggg cttgtagcag ccggatctca gtggtggtgg tggtggtgct cgagtgcggc
       61 cgcaagcttg tcgactcatt tggggtaagc ttccacaatc atagcatgac cctgtcctcc
      121 agctgcacag gcagccacta aagcatactg gcctccatcc ttcctcagtc tgttggcagc
      181 tgccatgacc agccgacagc cagtggctcc aaaagggtgc cccagagaca gtgatccgcc
      241 ccagatatta aacttctcca gaggaggtga tccaaccttg gttttcctac ccatgtagtt
      301 ttgtgcaaac caatcagaat ccatggcttt aaagttagct aaaatctggc ctgagaaggc
      361 ttcatgaaat tcaaaagcat cgatatcatt catggttaac cctgcctttt ctaaaacttt
      421 tggagtagca tatgttggtc cgagtaaaag ctgatctttc ggatcctggg acacatatat
      481 aaaatccctc aaatatgcct ttggtttata acccatggcc agagctctgt cctctgacat
      541 gattagcatc gcagaagcgc catcagtcag gaaagaagaa tttgcagctg tcactgtgcc
      601 atagggtttg atgaacgcag gttttagttt ggccatttgc tccagtgagg aaggacggat
      661 cccattatct ttggtaactg tgtcctttcc tggtactttg aagggtacaa tatcagaaag
      721 gtgtccttca tcctgtgcct tcttggccag actatgagaa cgcagtgc
//
