LOCUS       sequence-180441-         723 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses-flag tagged mouse ECHA(37-768) K351R in E.coli cells (no 
            His-tag).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 723)
  AUTHORS   Sadhukhan S, Liu X, Ryu D, Nelson OD, Stupinski JA, Li Z, Chen W, 
            Zhang S, Weiss RS, Locasale JW, Auwerx J, Lin H
  TITLE     Metabolomics-assisted proteomics identifies succinylation and SIRT5 
            as important regulators of cardiac function.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 Apr 19;113(16):4320-5. doi: 
            10.1073/pnas.1519858113. Epub 2016 Apr 5.
  PUBMED    27051063
REFERENCE   2  (bases 1 to 723)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1519858113"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2016-04-19- 19"; volume: "113"; issue: "16"; pages: "4320-5"
COMMENT     Sequence Label: pET-Duet-ECHA(37-763) K351R-FLAG (no His-tag)
FEATURES             Location/Qualifiers
     source          1..723
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(7..51)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             complement(67..90)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 tgtagacgag tccatgtgct ggcgttcaaa tttcgcagca gcggtttctt taccagactc
       61 gagtcactta tcgtcgtcat ccttgtaatc ctggtagaac ttcttgctag agttgttagc
      121 gtggtcgagg agcagctggc atggggtaaa ctgtgttcca taggcagact cgtacttccg
      181 tagccggtcc actaccttct gagcaccata cagatccaca aagcggaagg gccctccgag
      241 acaaggggga aagccaagcc caaagacagc tccgatgtct ccctctgccg gtgtggccag
      301 gatcccttcc tgtaggcaca ggactgcctc gttcacgaac cttgttatca cacggtattg
      361 gacgtcttca tcagaggaga cctcgggctt ggcaggcagc ctcagatttg ccaagatatt
      421 atccatttca gaattcaaac tcttattctt tgagccctcc tgatagatgt aaaagccctt
      481 cccagacttg cgacctaaga agcccttgga gaccatctgt ttcagcagtt ctacgctgcc
      541 acctccaaat cgctccccaa aggctttgcc tagatcttct gctacgtgct gtgctacatc
      601 cacacccact tcgtctgcca gcgtggcagc acccacaggg aagccgaaac ctgtggtcaa
      661 ggcgtccagc ttcttagggt caactccttc ctggaggatt cgcatgactt cagacatcat
      721 ggg
//
