LOCUS       sequence-180444-         728 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses flag-tagged mouse ECHA (37-768) in E.coli cells (no 
            His-tag).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 728)
  AUTHORS   Sadhukhan S, Liu X, Ryu D, Nelson OD, Stupinski JA, Li Z, Chen W, 
            Zhang S, Weiss RS, Locasale JW, Auwerx J, Lin H
  TITLE     Metabolomics-assisted proteomics identifies succinylation and SIRT5 
            as important regulators of cardiac function.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 Apr 19;113(16):4320-5. doi: 
            10.1073/pnas.1519858113. Epub 2016 Apr 5.
  PUBMED    27051063
REFERENCE   2  (bases 1 to 728)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1519858113"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2016-04-19- 19"; volume: "113"; issue: "16"; pages: "4320-5"
COMMENT     Sequence Label: pET-Duet-ECHA(37-763)-FLAG (no His-tag)
FEATURES             Location/Qualifiers
     source          1..728
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(4..48)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             complement(64..87)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 agacgagtcc atgtgctggc gttcaaattt cgcagcagcg gtttctttac cagactcgag
       61 tcacttatcg tcgtcatcct tgtaatcctg gtagaacttc ttgctagagt tgttagcgtg
      121 gtcgaggagc agctggcatg gggtaaactg tgttccatag gcagactcgt acttccgtag
      181 ccggtccact accttctgag caccatacag atccacaaag cggaagggcc ctccgagaca
      241 agggggaaag ccaagcccaa agacagctcc gatgtctccc tctgccggtg tggccaggat
      301 cccttcctgt aggcacagga ctgcctcgtt cacgaacctt gttatcacac ggtattggac
      361 gtcttcatca gaggagacct cgggcttggc aggcagcctc agatttgcca agatattatc
      421 catttcagaa ttcaaactct tattctttga gccctcctga tagatgtaaa agcccttccc
      481 agacttgcga cctaagaagc ccttggagac catctgtttc agcagttcta cgctgccacc
      541 tccaaatcgc tccccaaagg ctttgcctag atcttctgct acgtgctgtg ctacatccac
      601 acccacttcg tctgccagcg tggcagcacc cacagggaag ccgaaacctg tggtcaaggc
      661 gtccagcttc ttagggtcaa ctccttcctg gaggattcgc atgacttcag acatcatggg
      721 tgcaagac
//
