LOCUS       sequence-180490-         675 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  photoconvertible fluorescent protein pcDronpa2-A69T in pRSetB 
            plasmid, photoconvertible by primed conversion mechanism, bacterial 
            overexpression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 675)
  AUTHORS   Turkowyd B, Balinovic A, Virant D, Golz Carnero HG, Caldana F, 
            Endesfelder M, Bourgeois D, Endesfelder U
  TITLE     A general mechanism of photoconversion of green-to-red fluorescent 
            proteins based on blue and infrared light reduces phototoxicity in 
            live-cell single-molecule imaging.
  JOURNAL   Angew Chem Int Ed Engl. 2017 Jun 2. doi: 10.1002/anie.201702870.
  PUBMED    28574633
REFERENCE   2  (bases 1 to 675)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Angew Chem 
            Int Ed Engl. 2017 Jun 2. doi: 10.1002/anie.201702870."
COMMENT     Sequence Label: pRSETb-pcDronpa2-A69T
FEATURES             Location/Qualifiers
     source          1..675
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..675
                     /codon_start=1
                     /product="monomeric photoswitchable Dronpa-Green1 
                     fluorescent protein"
                     /label=Dronpa-Green1
                     /translation="MSVIKPDMKIKLRMEGAVNGHPFAIEGVGLGKPFEGKQSMDLKVK
                     EGGPLPFAYDILTTAFHYGNRVFTKYPENIVDYFKQSFPEGYSWERSMSYEDGGICIAT
                     NDITLDGDCYINEIRFDGVNFPANGPVMQKRTVKWEPSTEKLYVRDGVLKGDVNMALSL
                     EGGGHYRCDFKTTYKAKKVVQLPDYHFVDHHIEIKSHDKDYSNVNLHEHAEAHSGLPRQ
                     AK"
ORIGIN
        1 atgagtgtga ttaaaccaga catgaagatc aagctgcgta tggaaggcgc cgtaaatgga
       61 cacccgttcg cgattgaagg agttggcctt gggaagcctt tcgagggaaa acagagtatg
      121 gaccttaaag tcaaagaagg cggacctctg cctttcgcct atgacatctt gacaactgcg
      181 ttccattacg gcaacagggt attcactaaa tacccagaaa atatagtaga ctatttcaag
      241 cagtcgtttc ctgagggcta ctcttgggaa cgaagcatga gttacgaaga cgggggcatt
      301 tgtatcgcga caaacgacat aaccctggat ggtgactgtt atatcaacga aattcgattt
      361 gatggtgtga actttcctgc caatggtcca gttatgcaga agaggactgt gaaatgggag
      421 ccatccactg agaaattgta tgtgcgtgat ggagtgctga agggtgatgt taacatggct
      481 ctgtcgcttg aaggaggtgg ccattaccga tgtgacttca aaactactta taaagctaag
      541 aaggttgtcc agttgccaga ctaccacttt gtggaccacc acattgagat taaaagccac
      601 gacaaagatt acagtaatgt taatctgcac gagcatgccg aagcgcattc tgggctgccg
      661 aggcaggcca agtaa
//
