LOCUS       sequence-180503-         684 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  photoconvertible fluorescent protein mEos4b-V69T in pRSetA plasmid, 
            photoconvertible by primed conversion mechanism, bacterial 
            overexpression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 684)
  AUTHORS   Turkowyd B, Balinovic A, Virant D, Golz Carnero HG, Caldana F, 
            Endesfelder M, Bourgeois D, Endesfelder U
  TITLE     A general mechanism of photoconversion of green-to-red fluorescent 
            proteins based on blue and infrared light reduces phototoxicity in 
            live-cell single-molecule imaging.
  JOURNAL   Angew Chem Int Ed Engl. 2017 Jun 2. doi: 10.1002/anie.201702870.
  PUBMED    28574633
REFERENCE   2  (bases 1 to 684)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Angew Chem 
            Int Ed Engl. 2017 Jun 2. doi: 10.1002/anie.201702870."
COMMENT     Sequence Label: pRSETa-mEos4b-V69T
FEATURES             Location/Qualifiers
     source          1..684
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..684
                     /codon_start=1
                     /product="fixation-resistant truly monomeric green-to-red 
                     photoswitchable fluorescent protein (Paez-Segala et al., 
                     2015)"
                     /label=mEos4b
                     /note="derived from mEos2"
                     /translation="MVSAIKPDMRIKLRMEGNVNGHHFVIDGDGTGKPYEGKQTMDLEV
                     KEGGPLPFAFDILTTAFHYGNRVFTKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICNA
                     RNDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIEMALL
                     LEGNAHYRCDFRTTYKAKEKGVKLPGAHFVDHAIEILSHDKDYNKVKLYEHAVAHSGLP
                     DNARR"
ORIGIN
        1 atggtgagtg cgattaagcc agacatgagg atcaaactcc gtatggaagg caacgtaaac
       61 gggcaccact ttgtgatcga cggagatggt acaggcaagc cttatgaggg aaaacagacc
      121 atggatcttg aagtcaaaga gggcggacct ctgccttttg cctttgatat cctgaccact
      181 gcattccatt acggcaacag ggtattcacg aaatatccag acaacataca agactatttt
      241 aagcagtcgt ttcctaaggg gtattcgtgg gaacgaagct tgactttcga agacgggggc
      301 atttgcaatg ccagaaacga cataacaatg gaaggggaca ctttctataa taaagttcga
      361 ttttatggta ccaactttcc cgccaatggt ccagttatgc agaagaagac gctgaaatgg
      421 gagccctcca ctgagaaaat gtatgtgcgt gatggagtgc tgacgggtga tattgagatg
      481 gctttgttgc ttgaaggaaa tgcccattac cgatgtgact tcagaactac ttacaaagct
      541 aaggagaagg gtgtcaagtt accaggcgcc cactttgtgg accacgccat tgagatttta
      601 agccatgaca aagattacaa caaggttaag ctgtatgagc atgctgttgc tcattctgga
      661 ttgcctgaca atgccagacg ataa
//
