LOCUS       sequence-180510-        3280 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  NAT-marked pSNR52 promoter fragment for use in gRNA expression 
            cassette stitching PCR; for use with pADH119, 139, or 147 to 
            generate target-specific gRNA expression cassette..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3280)
  AUTHORS   Nguyen N, Quail MMF, Hernday AD
  TITLE     An Efficient, Rapid, and Recyclable System for CRISPR-Mediated 
            Genome Editing in Candida albicans.
  JOURNAL   mSphere. 2017 Apr 26;2(2):e00149-17. doi: 
            10.1128/mSphereDirect.00149-17. eCollection 2017 Mar-Apr.
  PUBMED    28497115
REFERENCE   2  (bases 1 to 3280)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/mSphereDirect.00149-17"; journalName: "mSphere"; date: 
            "2017-04-26- 26"; volume: "2"; issue: "2"; pages: "e00149-17"
COMMENT     Sequence Label: pADH110
FEATURES             Location/Qualifiers
     source          1..3280
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     111..130
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(349..453)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     521..539
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      1110..1307
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     promoter        complement(join(1655..1661,1662..1679,1680..1685))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1700..1721
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1838..1855)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2009..2597)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2089..2108)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2768..3280,1..279,280..348))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
       61 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
      121 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
      181 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
      241 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
      301 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
      361 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
      421 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
      481 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc
      541 ctttgacggc acggccacgc gtttaaaccg ccaggtgatg ctgaagctat tgaagctttg
      601 gatggttctt tcactaccga tactgttttt agagttactg ctactggtga tggtttcact
      661 ttgagagaag ttccagttga tccaccattg actaaggttt tcccagatga tgaatccgat
      721 gatgaatccg atgctggtga agatggtgat ccagattcta gaactttcgt tgcttatggt
      781 gatgatggtg atttggctgg tttcgttgtt gtttcttatt ctggttggaa cagaagattg
      841 actgttgaag atattgaagt tgctccagaa catagaggtc atggtgttgg tagagctttg
      901 atgggtttgg ctactgaatt cgccagagaa agaggtgctg gtcatttgtg gttggaagtt
      961 accaatgtta atgctccagc tattcatgct tatagaagaa tgggtttcac tttgtgtggt
     1021 ttggatactg ctttatacga tggtactgct tccgatggtg aacaagcttt gtatatgtct
     1081 atgccatgtc cataataact cgagtcagta ctgacaataa aaagattctt gttttcaaga
     1141 acttgtcatt tgtatagttt ttttatattg tagttgttct attttaatca aatgttagcg
     1201 tgatttatat tttttttcgc ctcgacatca tctgcccaga tgcgaagtta agtgcgcaga
     1261 aagtaatatc atgcgtcaat cgtatgtgaa tgctggtcgc tatactgctg tcgattcgat
     1321 actaacatct aatcaactcc cagatcacca ctatacattt aaaagtcatt ggtcaataac
     1381 tatactcgag tattgcctca tcaaagaaac aatcaaatat tatagatact cactccatca
     1441 cgtgataatt tcactggtat ggaaaagtgg aaaattttat aaaaaaaaat ttgatgcctt
     1501 tggcatagct gaaacttcgg cccaatagga ttggagaata tgttttcgca gcgttcttac
     1561 aattaaattg tggtggaagt tcgagacttg cgtaaactat ttttaatttg cggtgtttaa
     1621 accccagcgc ctggcgggcg ctcacaattc cacacaacat acgagccgga agcataaagt
     1681 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     1741 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     1801 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     1861 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     1921 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     1981 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     2041 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     2101 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     2161 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     2221 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     2281 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     2341 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     2401 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     2461 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     2521 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     2581 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     2641 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     2701 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     2761 ctgacagtta ccaatgcttg atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     2821 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     2881 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     2941 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     3001 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     3061 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     3121 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     3181 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     3241 tatcactcat ggttatggca gcactgcata attctcttac
//
