LOCUS       sequence-180521-        9185 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  NAT-marked CAS9 expression construct; part 1 of 2 of C.mal LEUpOUT 
            CRISPR system. Use with pADH143-series gRNA expression constructs..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9185)
  AUTHORS   Nguyen N, Quail MMF, Hernday AD
  TITLE     An Efficient, Rapid, and Recyclable System for CRISPR-Mediated 
            Genome Editing in Candida albicans.
  JOURNAL   mSphere. 2017 Apr 26;2(2):e00149-17. doi: 
            10.1128/mSphereDirect.00149-17. eCollection 2017 Mar-Apr.
  PUBMED    28497115
REFERENCE   2  (bases 1 to 9185)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/mSphereDirect.00149-17"; journalName: "mSphere"; date: 
            "2017-04-26- 26"; volume: "2"; issue: "2"; pages: "e00149-17"
COMMENT     Sequence Label: pADH140
FEATURES             Location/Qualifiers
     source          1..9185
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     205..224
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(443..547)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     615..633
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             2201..6304
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             2201..6304
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             6317..6337
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6341..6361
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6365..6385
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6386..6451
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     terminator      6516..6763
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(6532..6550)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     promoter        6803..7146
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     promoter        complement(join(7654..7660,7661..7678,7679..7684))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7699..7720
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7837..7854)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8008..8596)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8088..8107)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8767..9185,1..373,374..442))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
       61 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
      121 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
      181 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
      241 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
      301 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
      361 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
      421 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
      481 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
      541 ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga
      601 cattaaccta taaaaatagg cgtatcacga ggccctttga cggcacggcc acgcgtttaa
      661 accgccatgt cagttaaaac taaaactatt acaattttac caggtgatca cgtcggtacc
      721 gaaattgtca atgaagctat caaagtttta gaagcaattg aagctgccac tccttaccaa
      781 aaaatccatt tcgatttcaa acatcattta atcggtggtg ctgccattga tgccactggt
      841 gtcccattgc cagatgacgc acttgaaagt gccaaaaaat ctgatgccgt cttattgggt
      901 gctgttggtg gaccaaaatg ggggactggc gccgttcgtc cagaacaagg tttattaaag
      961 attcgtaaag aattgaacct ttatgctaac atcagaccat gtaactttgc cagtgactcc
     1021 ttattggaat tatccccatt gagaccagaa gtcgttaaag gtacaaactt gatcattgtt
     1081 cgtgaattag ttggtggtat ttatttcggt gatcgtgaag aacaagaaga aagtgaagat
     1141 aaacaaaccg catgggatac tgaaaaatac accgtcgatg aagtcaccag aatcacccgt
     1201 atggctgcct tcatggcttt acaacataac ccaccattac caatctggtc attggataag
     1261 gctaatgttt tagcttcttc tagattatgg agaagaactg tcgataaagt gatttctgaa
     1321 gagttcccaa ctttgtctgt tcaacatcaa ttgattgact ccgccgccat gattttaatt
     1381 caaaacccaa ccaaattgaa tggtataatc atcactacag ctaacaatta cacaaaaaaa
     1441 attctctttt cgatgtagca actatcttca actaaaacat tttctccttc ggcccatgat
     1501 tgtcctccgg gtcgacagca agccgttaca attgagatgg aaagcgacct accttcactc
     1561 gataaggtgc ttaattgtac ttcatataaa tctggcccgg atctaaacaa atgagttcca
     1621 ttaagccgtg ggttctcaat tagggttttt gtttttgatt tagaaaaaag agatcaagat
     1681 ttgtttacag gtgatgcctt tttttagaac ttatgcgttg caaaagttga ctaacgattt
     1741 ctataaggtg atccacacta attatacaaa cgtacaaaca gacatacttt tcctgcgttc
     1801 acctgatgtt ggccagattt ctctcttcat tgcatagaac ataaccacac tagggcaaca
     1861 gaaaaaaaaa aaaaagtgca tcgggaagtt gtgttccatt cattatatgt ctactactgc
     1921 atatgagtag cccacccacc accaccatag taagtttttg tgtatgcgcg ccgtcaggtt
     1981 atttcatttc tgaatttttc aaccacctta ctccctttat tgttgattga caattttgct
     2041 cacagtaaga tcttttagac tccaattaat ataaaataag tctgattttc caattcctgt
     2101 tttttctttt tttttctgtt tctatttctt tccttttctc cctttttttt aattcttcat
     2161 tcaatcatca attgataatt caggaatatt acaacccggg atggataaaa agtatagtat
     2221 tggtttagat attggtacta actctgtggg ttgggcagtt atcactgacg aatataaagt
     2281 tccatcaaag aaatttaagg tgttaggtaa cactgacaga cactcaataa aaaagaatct
     2341 tatcggtgct cttttgttcg actccggtga aactgccgag gctacacgtt taaaaagaac
     2401 agcaagaaga agatataccc gtagaaaaaa tagaatatgt tatttacaag aaatcttttc
     2461 taatgaaatg gctaaagttg atgattcctt tttccataga ttggaagagt catttttggt
     2521 tgaagaagac aaaaagcatg agagacatcc aatctttggg aatatagttg atgaagtggc
     2581 ttaccatgaa aaatatccta ccatttatca tttaagaaag aaattggtag attcaactga
     2641 taaagctgac cttagattaa tctatttagc acttgcccat atgattaaat ttagaggtca
     2701 ttttttgatt gaaggtgatt tgaacccaga taattctgac gtggataaat tatttattca
     2761 attagtccaa acctacaacc aattatttga ggaaaatcca attaatgcta gtggtgtcga
     2821 tgccaaagct atattatcag ccagattatc aaaatctaga cgtttggaaa atttgattgc
     2881 ccaattgcca ggagaaaaaa agaatggatt atttggaaac ttgatcgcat tatcattggg
     2941 tttgacacca aattttaaat ctaattttga tttagctgaa gatgctaaat tacaattatc
     3001 aaaagacacc tatgacgacg atttggacaa tttacttgct caaattggtg atcaatatgc
     3061 agatttgttc ttagctgcta aaaacttatc tgatgctatt ttgttgtctg atattttgag
     3121 agtgaacaca gaaataacca aagctccatt atcagcatct atgatcaaac gttatgatga
     3181 acaccatcag gatttgactt tattgaaagc tttggtgaga caacaattgc cagagaagta
     3241 taaagaaatc tttttcgatc aatctaaaaa cgggtatgca ggttatattg atgggggtgc
     3301 ctcccaagag gaattttaca aatttataaa acctatttta gaaaagatgg atgggactga
     3361 ggaacttttg gtcaaattga acagagaaga tttgttacgt aaacagagaa cttttgataa
     3421 tggtagtata cctcaccaaa ttcatttggg tgagttgcat gcaattttaa gaagacaaga
     3481 agatttttat ccatttttaa aagataatag agaaaaaatc gagaaaattt taacctttag
     3541 aattccatac tatgttgggc ctttggctag aggtaattca agatttgcct ggatgacacg
     3601 taaatcagaa gaaactatta ccccttggaa ttttgaagag gttgttgata aaggagcatc
     3661 agcacagagt tttattgaaa gaatgaccaa tttcgataaa aacttaccaa atgaaaaagt
     3721 tttaccaaaa cattccttgt tatacgaata ttttactgtt tacaatgaac ttacaaaggt
     3781 taaatatgtt actgaaggta tgcgtaagcc agccttttta tctggagaac agaaaaaggc
     3841 aatagttgat ttattgttta aaacaaatag aaaagttact gttaaacaat taaaagaaga
     3901 ttactttaag aaaattgaat gttttgattc agttgaaatc agtggtgttg aagacagatt
     3961 taatgctagt ttaggaactt accatgattt acttaaaatt atcaaagata aagatttctt
     4021 ggataacgaa gaaaatgaag acattttaga agacattgtt ttaaccttaa ctttattcga
     4081 agatagagag atgattgaag aacgtttgaa gacttatgca catttgtttg acgataaagt
     4141 gatgaaacag ttgaaaagaa gacgttatac tggatggggt agattgtctc gtaaattgat
     4201 caatggaatt agagataaac aaagtggtaa aactatcttg gactttttga aatctgacgg
     4261 atttgctaat agaaatttca tgcaattgat ccacgacgat agtttgacat ttaaagaaga
     4321 catccaaaag gcccaagtga gtgggcaagg tgattcatta catgaacata ttgcaaattt
     4381 agccggatct cctgctatta agaaagggat attacaaact gttaaagttg tggatgaatt
     4441 agtgaaagta atgggaagac ataaacctga aaacattgtc attgagatgg caagagaaaa
     4501 tcaaactaca caaaaaggac agaaaaatag tagagaacgt atgaaaagaa tagaagaggg
     4561 tattaaagaa ttgggtagtc aaatattgaa agaacaccca gtggaaaata cccagttgca
     4621 aaatgaaaaa ttatatcttt actaccttca aaatggacgt gatatgtatg ttgatcagga
     4681 attagatata aatagacttt cagattatga tgtagatcat atagttccac aatctttctt
     4741 gaaagatgat tccatagaca ataaagtatt aactagaagt gataaaaata gaggtaaaag
     4801 tgataatgtc ccaagtgagg aagtcgtcaa aaagatgaaa aattactggc gtcaactttt
     4861 gaatgctaaa ttaattactc aaagaaaatt tgataatttg actaaagcag aaagaggtgg
     4921 gctttctgaa ttagataaag ccgggttcat taaaagacaa ttggtcgaaa ctagacaaat
     4981 tactaaacat gttgcccaaa ttttagattc ccgtatgaac actaagtatg acgaaaatga
     5041 taagttaata cgtgaggtta aagtcattac tttaaaatca aaacttgtct ctgatttcag
     5101 aaaggatttc caattctata aagttagaga aattaataat tatcatcatg ctcatgatgc
     5161 atatttgaat gctgtagttg gaactgcttt aatcaagaaa taccctaaat tagaatctga
     5221 atttgtatat ggtgattaca aagtctatga tgttagaaag atgattgcta aatcagaaca
     5281 agaaattggt aaagctacag ctaaatactt cttttactct aacattatga atttctttaa
     5341 aacagaaatt actttggcaa acggtgaaat tagaaaaaga cctcttattg aaacaaatgg
     5401 tgagactgga gagatagttt gggacaaagg gcgtgatttc gctactgtta gaaaagtttt
     5461 atcaatgcca caagttaaca ttgtaaagaa aacagaggtt caaactggtg gtttctcaaa
     5521 agaaagtatt ttgcctaaaa gaaatagtga taaattgatt gccagaaaaa aggattggga
     5581 tccaaagaaa tatggtggtt tcgactcacc aaccgtagcc tattctgttt tggttgtggc
     5641 aaaggttgaa aagggtaaaa gtaaaaagct taaatcagta aaagaacttt tgggtattac
     5701 aataatggaa agaagttcct ttgaaaagaa ccctattgat tttttggaag ctaaaggtta
     5761 taaggaagta aagaaggact taataatcaa attgcctaaa tattctttat ttgaattaga
     5821 aaatgggaga aaaagaatgt tggcttctgc tggagaattg caaaagggta atgaattagc
     5881 attgccttcc aaatatgtta acttcttgta tttagcttca cactatgaaa agttgaaagg
     5941 gtcaccagaa gataacgagc aaaaacaatt atttgttgaa caacacaaac actacttaga
     6001 tgagattata gaacaaatta gtgaattcag taaaagagtg atattagctg atgcaaattt
     6061 agataaagtt ttgtcagcct ataacaaaca tagagataag ccaattagag aacaagcaga
     6121 aaacattatt cacttattta cccttaccaa tttaggagca cctgctgctt tcaagtattt
     6181 tgatacaaca attgatcgta aaagatatac ctcaacaaaa gaagtcttag acgccacctt
     6241 aattcatcaa tcaatcactg gattgtatga gacaagaatt gatttgtctc aattgggtgg
     6301 tgatgaaggg gctgatccta agaagaaaag aaaagttgat ccaaagaaaa agcgtaaggt
     6361 ggatcctaag aaaaagagaa aggttgacta caaagaccat gacggtgatt ataaagatca
     6421 tgacatcgac tacaaggatg acgatgacaa gtgataatga ctgcagagat ccatcgacct
     6481 gccgccaagc taattccggg cgaatttctg tcgagtcatg taattagtta tgtcacgctt
     6541 acattcacgc cctcccccca catccgctct aaccgaaaag gaaggagtta gacaacctga
     6601 agtctaggtc cctatttatt tttttatagt tatgttagta ttaagaacgt tatttatatt
     6661 tcaaattttt cttttttttc tgtacagacg cgtgtacgca tgtaacatta tactgaaaac
     6721 cttgctttag aaggttttgg gacgctcgaa ggctttaatt tgcggccagc ttgcctcgtc
     6781 cccgcctggt cacccggcca gcgacatgga ggcccagaat accctccttg acagtcttga
     6841 cgtgcgcagc tcaggggcat gatgtgactg tcgcccgtac atttagccca tacatcccca
     6901 tgtataatca tttgcatcca tacattttga tggccgcacg gcgcgaagca aaaattacgg
     6961 ctcctcgctg cagacctgcg agcagggaaa cgctcccctc acagacgcgt tgaattgtcc
     7021 ccacgccgcg cccctgtaga gaaatataaa aggttaggat ttgccactga ggttcttctt
     7081 tcatatactt ccttttaaaa tcttgctagg atacagttct cacatcacat ccgaacataa
     7141 acaaccggat cctacaatta tgtctactac tttggatgat actgcttata gatacagaac
     7201 ttctgttcca ggtgatgctg aagctattga agctttggat ggttctttca ctaccgatac
     7261 tgtttttaga gttactgcta ctggtgatgg tttcactttg agagaagttc cagttgatcc
     7321 accattgact aaggttttcc cagatgatga atccgatgat gaatccgatg ctggtgaaga
     7381 tggtgatcca gattctagaa ctttcgttgc ttatggtgat gatggtgatt tggctggttt
     7441 cgttgttgtt tcttattctg gttggaacag aagattgact gttgaagata ttgaagttgc
     7501 tccagaacat agaggtcatg gtgttggtag agctttgatg ggtttggcta ctgaattcgc
     7561 cagagaaaga ggtgctggtc atttgtggtt ggaagttacc aatgttaatc ggtgtttaaa
     7621 ccccagcgcc tggcgggcgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     7681 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     7741 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     7801 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     7861 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     7921 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     7981 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     8041 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     8101 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     8161 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     8221 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     8281 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     8341 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     8401 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     8461 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     8521 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     8581 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     8641 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     8701 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     8761 tgacagttac caatgcttga tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     8821 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     8881 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     8941 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     9001 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     9061 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     9121 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     9181 aaaag
//
