LOCUS       sequence-18054-e         629 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 629)
  AUTHORS   Donnenberg MS, Kaper JB
  TITLE     Construction of an eae deletion mutant of enteropathogenic 
            Escherichia coli by using a positive-selection suicide vector.
  JOURNAL   Infect Immun. 1991 Dec . 59(12):4310-7.
  PUBMED    1937792
REFERENCE   2  (bases 1 to 629)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Infect 
            Immun. 1991 Dec . 59(12):4310-7."
COMMENT     Sequence Label: pCVD442
FEATURES             Location/Qualifiers
     source          1..629
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    43..59
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        185..629
                     /gene="sacR"
                     /label=sacB promoter
                     /note="sacB promoter and control region"
ORIGIN
        1 ggctgacatg ggaattccac atgtggaatt ccacatgtgg aattgtgagc ggataacaat
       61 ttgtggaatt cccgggagag ctcgatatcg catgcggtac ctctagaaga agcttgggat
      121 ccgtcgacct gcagatccgt cgacctgcag gtcgactcta gaggatcgat cctttttaac
      181 ccatcacata tacctgccgt tcactattat ttagtgaaat gagatattat gatattttct
      241 gaattgtgat taaaaaggca actttatgcc catgcaacag aaactataaa aaatacagag
      301 aatgaaaaga aacagataga ttttttagtt ctttaggccc gtagtctgca aatcctttta
      361 tgattttcta tcaaacaaaa gaggaaaata gaccagttgc aatccaaacg agagtctaat
      421 agaatgaggt cgaaaagtaa atcgcgcggg tttgttactg ataaagcagg caagacctaa
      481 aatgtgtaaa gggcaaagtg tatactttgg cgtcacccct tacatatttt aggtcttttt
      541 ttattgtgcg taactaactt gccatcttca aacaggaggg ctggaagaag cagaccgcta
      601 acacagtaca taaaaaagga gacatgaac
//
