LOCUS       sequence-180556-        9333 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expresses 7D mutant BRD4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9333)
  AUTHORS   Shu S, Lin CY, He HH, Witwicki RM, Tabassum DP, Roberts JM, 
            Janiszewska M, Huh SJ, Liang Y, Ryan J, Doherty E, Mohammed H, Guo 
            H, Stover DG, Ekram MB, Peluffo G, Brown J, D'Santos C, Krop IE, 
            Dillon D, McKeown M, Ott C, Qi J, Ni M, Rao PK, Duarte M, Wu SY, 
            Chiang CM, Anders L, Young RA, Winer EP, Letai A, Barry WT, Carroll 
            JS, Long HW, Brown M, Liu XS, Meyer CA, Bradner JE, Polyak K
  TITLE     Response and resistance to BET bromodomain inhibitors in 
            triple-negative breast cancer.
  JOURNAL   Nature. 2016 Jan 21;529(7586):413-7. doi: 10.1038/nature16508. Epub 
            2016 Jan 6.
  PUBMED    26735014
REFERENCE   2  (bases 1 to 9333)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature16508"; journalName: "Nature"; date: "2016-01-21- 
            21"; volume: "529"; issue: "7586"; pages: "413-7"
COMMENT     Sequence Label: pcDNA5-Flag-BRD4-7D
FEATURES             Location/Qualifiers
     source          1..9333
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        107..486
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        487..690
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     641..661
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    692..710
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    713..731
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     741..765
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             864..887
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3105..3122
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5157..5174)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5163..5387
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5520..5539)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    5671..5718
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             5726..6748
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             5726..6748
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    6878..6999
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6915..6934)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6969..6988
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7048..7064)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7048..7064)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7061..7083)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7072..7088
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7096..7102,7103..7120,7121..7126))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7141..7162
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7279..7296)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7450..8038)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7530..7549)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8209..9000,9001..9069))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8832..8851
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9070..9174)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9249..9268)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct gcttagggtt
       61 aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca ttgattattg
      121 actagttatt aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc
      181 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
      241 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
      301 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
      361 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
      421 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
      481 accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg
      541 ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa
      601 cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt
      661 gtacggtggg aggtctatat aagcagagct ctccctatca gtgatagaga tctccctatc
      721 agtgatagag atcgtcgacg agctcgttta gtgaaccgtc agatcgcctg gagacgccat
      781 ccacgctgtt ttgacctcca tagaagacac cgggaccgat ccagcctccg gactctagcg
      841 tttaaactta agcttccacc atggattaca aggatgacga cgataaggga tctatgtctg
      901 cggagagcgg ccctgggacg agattgagaa atctgccagt aatgggggat ggactagaaa
      961 cttcccaaat gtctacaaca caggcccagg cccaacccca gccagccaac gcagccagca
     1021 ccaacccccc gcccccagag acctccaacc ctaacaagcc caagaggcag accaaccaac
     1081 tgcaatacct gctcagagtg gtgctcaaga cactatggaa acaccagttt gcatggcctt
     1141 tccagcagcc tgtggatgcc gtcaagctga acctccctga ttactataag atcattaaaa
     1201 cgcctatgga tatgggaaca ataaagaagc gcttggaaaa caactattac tggaatgctc
     1261 aggaatgtat ccaggacttc aacactatgt ttacaaattg ttacatctac aacaagcctg
     1321 gagatgacat agtcttaatg gcagaagctc tggaaaagct cttcttgcaa aaaataaatg
     1381 agctacccac agaagaaacc gagatcatga tagtccaggc aaaaggaaga ggacgtggga
     1441 ggaaagaaac agggacagca aaacctggcg tttccacggt accaaacaca actcaagcat
     1501 cgactcctcc gcagacccag acccctcagc cgaatcctcc tcctgtgcag gccacgcctc
     1561 accccttccc tgccgtcacc ccggacctca tcgtccagac ccctgtcatg acagtggtgc
     1621 ctccccagcc actgcagacg cccccgccag tgccccccca gccacaaccc ccacccgctc
     1681 cagctcccca gcccgtacag agccacccac ccatcatcgc ggccacccca cagcctgtga
     1741 agacaaagaa gggagtgaag aggaaagcag acaccaccac ccccaccacc attgacccca
     1801 ttcacgagcc accctcgctg cccccggagc ccaagaccac caagctgggc cagcggcggg
     1861 agagcagccg gcctgtgaaa cctccaaaga aggacgtgcc cgactctcag cagcacccag
     1921 caccagagaa gagcagcaag gtctcggagc agctcaagtg ctgcagcggc atcctcaagg
     1981 agatgtttgc caagaagcac gccgcctacg cctggccctt ctacaagcct gtggacgtgg
     2041 aggcactggg cctacacgac tactgtgaca tcatcaagca ccccatggac atgagcacaa
     2101 tcaagtctaa actggaggcc cgtgagtacc gtgatgctca ggagtttggt gctgacgtcc
     2161 gattgatgtt ctccaactgc tataagtaca accctcctga ccatgaggtg gtggccatgg
     2221 cccgcaagct ccaggatgtg ttcgaaatgc gctttgccaa gatgccggac gagcctgagg
     2281 agccagtggt ggccgtgtcc tccccggcag tgccccctcc caccaaggtt gtggccccgc
     2341 ccgattccag cgacgacagc agcgatgcct ccgcggacag tgacgctgcg actgatgacg
     2401 ctgaggagga gcgagcccag cggctggctg agctccagga gcagctcaaa gccgtgcacg
     2461 agcagcttgc agccctctct cagccccagc agaacaaacc aaagaaaaag gagaaagaca
     2521 agaaggaaaa gaaaaaagaa aagcacaaaa ggaaagagga agtggaagag aataaaaaaa
     2581 gcaaagccaa ggaacctcct cctaaaaaga cgaagaaaaa taatagcagc aacagcaatg
     2641 tgagcaagaa ggagccagcg cccatgaaga gcaagccccc tcccacgtat gagtcggagg
     2701 aagaggacaa gtgcaagcct atgtcctatg aggagaagcg gcagctcagc ttggacatca
     2761 acaagctccc cggcgagaag ctgggccgcg tggtgcacat catccagtca cgggagccct
     2821 ccctgaagaa ttccaacccc gacgagattg aaatcgactt tgagaccctg aagccgtcca
     2881 cactgcgtga gctggagcgc tatgtcacct cctgtttgcg gaagaaaagg aaacctcaag
     2941 ctgagaaagt tgatgtgatt gccggctcct ccaagatgaa gggcttctcg tcctcagagt
     3001 cggagagctc cagtgagtcc agctcctctg acagcgaaga ctccgaaaca gagatggctc
     3061 cgaagtcaaa aaagaagggg caccccggga gggagcagaa gaagcaccat catcaccacc
     3121 atcagcagat gcagcaggcc ccggctcctg tgccccagca gccgcccccg cctccccagc
     3181 agcccccacc gcctccacct ccgcagcagc aacagcagcc gccacccccg cctcccccac
     3241 cctccatgcc gcagcaggca gccccggcga tgaagtcctc gcccccaccc ttcattgcca
     3301 cccaggtgcc cgtcctggag ccccagctcc caggcagcgt ctttgacccc atcggccact
     3361 tcacccagcc catcctgcac ctgccgcagc ctgagctgcc ccctcacctg ccccagccgc
     3421 ctgagcacag cactccaccc catctcaacc agcacgcagt ggtctctcct ccagctttgc
     3481 acaacgcact accccagcag ccatcacggc ccagcaaccg agccgctgcc ctgcctccca
     3541 agcccgcccg gcccccagcc gtgtcaccag ccttgaccca aacacccctg ctcccacagc
     3601 cccccatggc ccaacccccc caagtgctgc tggaggatga agagccacct gccccacccc
     3661 tcacctccat gcagatgcag ctgtacctgc agcagctgca gaaggtgcag ccccctacgc
     3721 cgctactccc ttccgtgaag gtgcagtccc agcccccacc ccccctgccg cccccacccc
     3781 acccctctgt gcagcagcag ctgcagcagc agccgccacc acccccacca ccccagcccc
     3841 agcctccacc ccagcagcag catcagcccc ctccacggcc cgtgcacttg cagcccatgc
     3901 agttttccac ccacatccaa cagcccccgc caccccaggg ccagcagccc ccccatccgc
     3961 ccccaggcca gcagccaccc ccgccgcagc ctgccaagcc tcagcaagtc atccagcacc
     4021 accattcacc ccggcaccac aagtcggacc cctactcaac cggtcacctc cgcgaagccc
     4081 cctccccgct tatgatacat tccccccaga tgtcacagtt ccagagcctg acccaccagt
     4141 ctccacccca gcaaaacgtc cagcctaaga aacaggagct gcgtgctgcc tccgtggtcc
     4201 agccccagcc cctcgtggtg gtgaaggagg agaagatcca ctcacccatc atccgcagcg
     4261 agcccttcag cccctcgctg cggccggagc cccccaagca cccggagagc atcaaggccc
     4321 ccgtccacct gccccagcgg ccggaaatga agcctgtgga tgtcgggagg cctgtgatcc
     4381 ggcccccaga gcagaacgca ccgccaccag gggcccctga caaggacaaa cagaaacagg
     4441 agccgaagac tccagttgcg cccaaaaagg acctgaaaat caagaacatg ggctcctggg
     4501 ccagcctagt gcagaagcat ccgaccaccc cctcctccac agccaagtca tccagcgaca
     4561 gcttcgagca gttccgccgc gccgctcggg agaaagagga gcgtgagaag gccctgaagg
     4621 ctcaggccga gcacgctgag aaggagaagg agcggctgcg gcaggagcgc atgaggagcc
     4681 gagaggacga ggatgcgctg gagcaggccc ggcgggccca tgaggaggca cgtcggcgcc
     4741 aggagcagca gcagcagcag cgccaggagc aacagcagca gcagcaacag caagcagctg
     4801 cggtggctgc cgccgccacc ccacaggccc agagctccca gccccagtcc atgctggacc
     4861 agcagaggga gttggcccgg aagcgggagc aggagcgaag acgccgggaa gccatggcag
     4921 ctaccattga catgaatttc cagagtgatc tattgtcaat atttgaagaa aatcttttct
     4981 gagcgcacct aggtggcttc tgactttgat tttctggcaa aacattgact ttccatagtg
     5041 ttaggggcgg tggtggaggt gggatcagcg gccaggggat gcctcagggc ctggccctcc
     5101 tgcatgcaag cttcagctgc tcgagtctag agggcccgtt taaacccgct gatcagcctc
     5161 gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac
     5221 cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg
     5281 tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga
     5341 ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga
     5401 aagaaccagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc
     5461 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     5521 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     5581 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     5641 acttgattag ggtgatggtt cacgtaccta gaagttccta ttccgaagtt cctattctct
     5701 agaaagtata ggaacttcct tggccaaaaa gcctgaactc accgcgacgt ctgtcgagaa
     5761 gtttctgatc gaaaagttcg acagcgtctc cgacctgatg cagctctcgg agggcgaaga
     5821 atctcgtgct ttcagcttcg atgtaggagg gcgtggatat gtcctgcggg taaatagctg
     5881 cgccgatggt ttctacaaag atcgttatgt ttatcggcac tttgcatcgg ccgcgctccc
     5941 gattccggaa gtgcttgaca ttggggaatt cagcgagagc ctgacctatt gcatctcccg
     6001 ccgtgcacag ggtgtcacgt tgcaagacct gcctgaaacc gaactgcccg ctgttctgca
     6061 gccggtcgcg gaggccatgg atgcgatcgc tgcggccgat cttagccaga cgagcgggtt
     6121 cggcccattc ggaccgcaag gaatcggtca atacactaca tggcgtgatt tcatatgcgc
     6181 gattgctgat ccccatgtgt atcactggca aactgtgatg gacgacaccg tcagtgcgtc
     6241 cgtcgcgcag gctctcgatg agctgatgct ttgggccgag gactgccccg aagtccggca
     6301 cctcgtgcac gcggatttcg gctccaacaa tgtcctgacg gacaatggcc gcataacagc
     6361 ggtcattgac tggagcgagg cgatgttcgg ggattcccaa tacgaggtcg ccaacatctt
     6421 cttctggagg ccgtggttgg cttgtatgga gcagcagacg cgctacttcg agcggaggca
     6481 tccggagctt gcaggatcgc cgcggctccg ggcgtatatg ctccgcattg gtcttgacca
     6541 actctatcag agcttggttg acggcaattt cgatgatgca gcttgggcgc agggtcgatg
     6601 cgacgcaatc gtccgatccg gagccgggac tgtcgggcgt acacaaatcg cccgcagaag
     6661 cgcggccgtc tggaccgatg gctgtgtaga agtactcgcc gatagtggaa accgacgccc
     6721 cagcactcgt ccgagggcaa aggaatagca cgtactacga gatttcgatt ccaccgccgc
     6781 cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca
     6841 gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa
     6901 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     6961 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac
     7021 ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     7081 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     7141 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     7201 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     7261 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     7321 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     7381 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     7441 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     7501 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     7561 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     7621 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     7681 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     7741 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     7801 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     7861 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     7921 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     7981 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     8041 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     8101 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     8161 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     8221 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     8281 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     8341 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     8401 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     8461 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     8521 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     8581 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     8641 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     8701 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     8761 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     8821 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     8881 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     8941 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     9001 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     9061 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     9121 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     9181 tccccgaaaa gtgccacctg acgtcgacgg atcgggagat ctcccgatcc cctatggtgc
     9241 actctcagta caatctgctc tgatgccgca tagttaagcc agtatctgct ccctgcttgt
     9301 gtgttggagg tcgctgagta gtgcgcgagc aaa
//
