LOCUS       sequence-180561-       10017 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses FLAG-HA-AGO2 [S824E, S828E, T830E, S831E, S834E (5xE)].
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10017)
  AUTHORS   Golden RJ, Chen B, Li T, Braun J, Manjunath H, Chen X, Wu J, Schmid 
            V, Chang TC, Kopp F, Ramirez-Martinez A, Tagliabracci VS, Chen ZJ, 
            Xie Y, Mendell JT
  TITLE     An Argonaute phosphorylation cycle promotes microRNA-mediated 
            silencing.
  JOURNAL   Nature. 2017 Feb 9;542(7640):197-202. doi: 10.1038/nature21025. Epub
            2017 Jan 23.
  PUBMED    28114302
REFERENCE   2  (bases 1 to 10017)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature21025"; journalName: "Nature"; date: "2017-02-9- 9"; 
            volume: "542"; issue: "7640"; pages: "197-202"
COMMENT     Sequence Label: pLJM1-FH-AGO2-5xE
FEATURES             Location/Qualifiers
     source          1..10017
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        131..334
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     285..305
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             419..442
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             443..469
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     regulatory      2405..2414
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    3100..3217
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3266..3776
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     3695..3713
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             3798..4397
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3798..3817)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4294..4314
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             4525..4758
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4830..4951
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4867..4886)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4921..4940
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4991..5126
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5146..5165)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5147..5165)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5175..5192)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5175..5191)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5184..5206)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5333..5788
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5420..5439)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5630..5651
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5814..5918
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5919..5987,5988..6779)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6137..6156)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6950..7538
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7439..7458
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7692..7709
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7826..7847
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7862..7867,7868..7885,7886..7892)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7900..7916
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7905..7927
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7924..7940
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7924..7940
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7959..7979
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7961..7979
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8007..8233
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8234..8414
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8461..8586
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9079..9312
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9497..9541
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     primer_bind     9839..9859
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
       61 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
      121 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
      181 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
      241 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
      301 gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta
      361 gcgctaccgg gtacaagtcc ggactcagat ctcgagctca agcttcgaat tcgccatgga
      421 ctacaaggac gacgatgaca agtaccctta tgacgtgccc gattacgcta gcggccgcat
      481 gtactcggga gccggccccg cacttgcacc tcctgcgccg ccgcccccca tccaaggata
      541 tgccttcaag cctccaccta gacccgactt tgggacctcc gggagaacaa tcaaattaca
      601 ggccaatttc ttcgaaatgg acatccccaa aattgacatc tatcattatg aattggatat
      661 caagccagag aagtgcccga ggagagttaa cagggaaatc gtggaacaca tggtccagca
      721 ctttaaaaca cagatctttg gggatcggaa gcccgtgttt gacggcagga agaatctata
      781 cacagccatg ccccttccga ttgggaggga caaggtggag ctggaggtca cgctgccagg
      841 agaaggcaag gatcgcatct tcaaggtgtc catcaagtgg gtgtcctgcg tgagcttgca
      901 ggcgttacac gatgcacttt cagggcggct gcccagcgtc ccttttgaga cgatccaggc
      961 cctggacgtg gtcatgaggc acttgccatc catgaggtac acccccgtgg gccgctcctt
     1021 cttcaccgcg tccgaaggct gctctaaccc tcttggcggg ggccgagaag tgtggtttgg
     1081 cttccatcag tccgtccggc cttctctctg gaaaatgatg ctgaatattg atgtgtcagc
     1141 aacagcgttt tacaaggcac agccagtaat cgagtttgtt tgtgaagttt tggattttaa
     1201 aagtattgaa gaacaacaaa aacctctgac agattcccaa agggtaaagt ttaccaaaga
     1261 aattaaaggt ctaaaggtgg agataacgca ctgtgggcag atgaagagga agtaccgtgt
     1321 ctgcaatgtg acccggcggc ccgccagtca ccaaacattc ccgctgcagc aggagagcgg
     1381 gcagacggtg gagtgcacgg tggcccagta tttcaaggac aggcacaagt tggttctgcg
     1441 ctacccccac ctcccatgtt tacaagtcgg acaggagcag aaacacacct accttcccct
     1501 ggaggtctgt aacattgtgg caggacaaag atgtattaaa aaattaacgg acaatcagac
     1561 ctcaaccatg atcagagcga ctgctaggtc ggcgcccgat cggcaagaag agattagcaa
     1621 attgatgcga agtgcaagtt tcaacacaga tccatacgtc cgtgaatttg gaatcatggt
     1681 caaagatgag atgacagacg tgactgggcg ggtgctgcag ccgccctcca tcctctacgg
     1741 gggcaggaat aaagctattg cgacccctgt ccagggcgtc tgggacatgc ggaacaagca
     1801 gttccacacg ggcatcgaga tcaaggtgtg ggccattgcg tgcttcgccc cccagcgcca
     1861 gtgcacggaa gtccatctga agtccttcac agagcagctc agaaagatct cgagagacgc
     1921 cggcatgccc atccagggcc agccgtgctt ctgcaaatac gcgcaggggg cggacagcgt
     1981 ggagcccatg ttccggcacc tgaagaacac gtatgcgggc ctgcagctgg tggtggtcat
     2041 cctgcccggc aagacgcccg tgtacgccga ggtcaagcgc gtgggagaca cggtgctggg
     2101 gatggccacg cagtgcgtgc agatgaagaa cgtgcagagg accacgccac agaccctgtc
     2161 caacctctgc ctgaagatca acgtcaagct gggaggcgtg aacaacatcc tgctgcccca
     2221 gggcaggccg ccggtgttcc agcagcccgt catctttctg ggagcagacg tcactcaccc
     2281 ccccgccggg gatgggaaga agccctccat tgccgccgtg gtgggcagca tggacgccca
     2341 ccccaatcgc tactgcgcca ccgtgcgcgt gcagcagcac cggcaggaga tcatacaaga
     2401 cctggccgcc atggtccgcg agctcctcat ccagttctac aagtccacgc gcttcaagcc
     2461 cacccgcatc atcttctacc gcgacggtgt ctctgaaggc cagttccagc aggttctcca
     2521 ccacgagttg ctggccatcc gtgaggcctg tatcaagcta gaaaaagact accagcccgg
     2581 gatcaccttc atcgtggtgc agaagaggca ccacacccgg ctcttctgca ctgacaagaa
     2641 cgagcgggtt gggaaaagtg gaaacattcc agcaggcacg actgtggaca cgaaaatcac
     2701 ccaccccacc gagttcgact tctacctgtg tagtcacgct ggcatccagg ggacaagcag
     2761 gccttcgcac tatcacgtcc tctgggacga caatcgtttc tcctctgatg agctgcagat
     2821 cctaacctac cagctgtgtc acacctacgt gcgctgcaca cgctccgtgt ccatcccagc
     2881 gccagcatac tacgctcacc tggtggcctt ccgggccagg taccacctgg tggataagga
     2941 acatgacgag gctgaaggag agcatgagga ggggcaggag aacgggcgag accaccaagc
     3001 actggccaag gcggtccagg ttcaccaaga cactctgcgc accatgtact ttgcttgaga
     3061 attctcgacc tcgagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     3121 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     3181 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     3241 agatccactt tggccgcggc tcgagggggt tggggttgcg ccttttccaa ggcagccctg
     3301 ggtttgcgca gggacgcggc tgctctgggc gtggttccgg gaaacgcagc ggcgccgacc
     3361 ctgggtctcg cacattcttc acgtccgttc gcagcgtcac ccggatcttc gccgctaccc
     3421 ttgtgggccc cccggcgacg cttcctgctc cgcccctaag tcgggaaggt tccttgcggt
     3481 tcgcggcgtg ccggacgtga caaacggaag ccgcacgtct cactagtacc ctcgcagacg
     3541 gacagcgcca gggagcaatg gcagcgcgcc gaccgcgatg ggctgtggcc aatagcggct
     3601 gctcagcagg gcgcgccgag agcagcggcc gggaaggggc ggtgcgggag gcggggtgtg
     3661 gggcggtagt gtgggccctg ttcctgcccg cgcggtgttc cgcattctgc aagcctccgg
     3721 agcgcacgtc ggcagtcggc tccctcgttg accgaatcac cgacctctct ccccaggggg
     3781 atccaccgga gcttaccatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg
     3841 acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc
     3901 acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca
     3961 cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg
     4021 tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca
     4081 tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc
     4141 cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc
     4201 agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg
     4261 gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg
     4321 gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc
     4381 gcaagcccgg tgcctgacgc ccgccccacg acccgcagcg cccgaccgaa aggagcgcac
     4441 gaccccatgc atcggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
     4501 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc
     4561 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     4621 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     4681 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     4741 tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg
     4801 caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca
     4861 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     4921 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct
     4981 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     5041 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     5101 gtagtgagga ggcttttttg gaggcctagg gacgtaccca attcgcccta tagtgagtcg
     5161 tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
     5221 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
     5281 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc
     5341 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     5401 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     5461 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     5521 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     5581 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     5641 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     5701 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     5761 tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa
     5821 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     5881 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     5941 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     6001 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     6061 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     6121 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     6181 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     6241 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     6301 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     6361 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     6421 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     6481 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     6541 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     6601 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     6661 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     6721 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     6781 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     6841 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     6901 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     6961 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     7021 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     7081 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     7141 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     7201 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     7261 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     7321 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     7381 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     7441 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     7501 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     7561 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     7621 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     7681 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     7741 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     7801 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
     7861 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
     7921 acacaggaaa cagctatgac catgattacg ccaagcgcgc aattaaccct cactaaaggg
     7981 aacaaaagct ggagctgcaa gcttaatgta gtcttatgca atactcttgt agtcttgcaa
     8041 catggtaacg atgagttagc aacatgcctt acaaggagag aaaaagcacc gtgcatgccg
     8101 attggtggaa gtaaggtggt acgatcgtgc cttattagga aggcaacaga cgggtctgac
     8161 atggattgga cgaaccactg aattgccgca ttgcagagat attgtattta agtgcctagc
     8221 tcgatacata aacgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     8281 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     8341 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     8401 gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag
     8461 ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga
     8521 ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag
     8581 agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca
     8641 gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga
     8701 ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag
     8761 ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca
     8821 accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag
     8881 atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc tgatcttcag
     8941 acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt
     9001 aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga
     9061 aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac
     9121 tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt
     9181 gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac
     9241 agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga
     9301 tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc
     9361 ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat
     9421 ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc
     9481 gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt
     9541 gtggaattgg tttaacataa caaattggct gtggtatata aaattattca taatgatagt
     9601 aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag
     9661 gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag
     9721 gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt
     9781 gaacggatct cgacggtatc gatcacgaga ctagcctcga gcggccgccc ccttcaccga
     9841 gggcctattt cccatgattc cttcatattt gcatatacga tacaaggctg ttagagagat
     9901 aattggaatt aatttgactg taaacacaaa gatattagta caaaatacgt gacgtagaaa
     9961 gtaataattt cttgggtagt ttgcagtttt aaaattatgt tttaaaatgg actatca
//
