LOCUS       sequence-18058-e         713 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 713)
  AUTHORS   Nallamsetty S, Austin BP, Penrose KJ, Waugh DS
  TITLE     Gateway vectors for the production of combinatorially-tagged 
            His6-MBP fusion proteins in the cytoplasm and periplasm of 
            Escherichia coli.
  JOURNAL   Protein Sci. 2005 Dec . 14(12):2964-71.
  PUBMED    16322578
REFERENCE   2  (bases 1 to 713)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Sci. 2005 Dec . 14(12):2964-71."
COMMENT     Sequence Label: pDEST-periHisMBP
FEATURES             Location/Qualifiers
     source          1..713
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             113..130
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gagcacttcc caacaaggac catagattat gaaaataaaa acaggtgcac gcatcctcgc
       61 attatccgca ttaacgacga tgatgttttc cgcctcggct ctcgccaaaa tccatcacca
      121 tcaccatcac gaagaaggta aactggtaat ctggattaac ggcgataaag gctataacgg
      181 tctcgctgaa gtcggtaaga aattcgagaa agataccgga attaaagtca ccgttgagca
      241 tccggataaa ctggaagaga aattcccaca ggttgcggca actggcgatg gccctgacat
      301 tatcttctgg gcacacgacc gctttggtgg ctacgctcaa tctggcctgt tggctgaaat
      361 caccccggac aaagcgttcc aggacaagct gtatccgttt acctgggatg ccgtacgtta
      421 caacggcaag ctgattgctt acccgatcgc tgttgaagcg ttatcgctga tttataacaa
      481 agatctgctg ccgaacccgc caaaaacctg ggaagagatc ccggcgctgg ataaagaact
      541 gaaagcgaaa ggtaagagcg cgctgatgtt caacctgcaa gaaccgtact tcacctggcc
      601 gctgattgct gctgacgggg gttatgcgtt caagtatgaa aacggcaagt acgacattaa
      661 agacgtgggc gtggataacg ctggcgcgaa agcgggtctg accttcctgg ttg
//
