LOCUS       sequence-180580-       10149 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of human Ng with deletion mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10149)
  AUTHORS   Hwang H, Szucs MJ, Ding LJ, Allen A, Ren X, Haensgen H, Gao F, Rhim 
            H, Andrade A, Pan JQ, Carr SA, Ahmad R, Xu W
  TITLE     Neurogranin, Encoded by the Schizophrenia Risk Gene NRGN, 
            Bidirectionally Modulates Synaptic Plasticity via 
            Calmodulin-Dependent Regulation of the Neuronal Phosphoproteome.
  JOURNAL   Biol Psychiatry. 2021 Feb 1;89(3):256-269. doi: 
            10.1016/j.biopsych.2020.07.014. Epub 2020 Jul 29.
  PUBMED    33032807
REFERENCE   2  (bases 1 to 10149)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.biopsych.2020.07.014"; journalName: "Biol Psychiatry"; 
            date: "2021-02-1- 1"; volume: "89"; issue: "3"; pages: "256-269"
COMMENT     Sequence Label: Ng-Delta-IQ FUNgDIQGW
FEATURES             Location/Qualifiers
     source          1..10149
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     41..62
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      104..113
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(355..1566)
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     complement(499..518)
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     misc_feature    1631..1748
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(2046..2090)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2275..2508
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    3001..3126
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             3173..3353
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(3371..3569)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(3396..3416)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        3571..3950
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     4122..4141
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        4216..4320
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4321..4389,4390..5181)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4539..4558)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5352..5940
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5841..5860
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6094..6111
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6228..6249
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6264..6269,6270..6287,6288..6294)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6302..6318
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6307..6329
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6326..6342
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6326..6342
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    6391..6512
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6402..6421)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6456..6475
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(6642..7016)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(7035..7082)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(7130..7459)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7144..7279
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7198..7217)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     7444..7464
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(7473..7901)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7583..7604)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7795..7814
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    7947..8171
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     8160..8177
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     LTR             8203..8383
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     8888..8904
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     8889..8905
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    8908..9496
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             9014..9025
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     9423..9443
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(join(9537..10149,1..101,102..104,105..107))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(9540..10149,1..101,102..104,105..107))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(9582..9603)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     9931..9950
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ccctcgccct cgccggacac gctgaacttg tggccgttta cgtcgccgtc cagctcgacc
       61 aggatgggca ccaccccggt gaacagctcc tcgcccttgc tcaccatggt ggcgaccggt
      121 acccggggat ccccgtctcc gctggggccg ccgcccgcgc ctccccgggc caccccagct
      181 ccgccaggcc ccccagggcc cgggcccttc cggccgcgct ctccgctctt tatggccgcg
      241 ttggcgccgg gatcgtccag cgggatgtct agaatgtcgt cgtccggctt ggagcaggcg
      301 ttctcggtgc agcagtccat ggatcctcta gagtcgacct gcagcccaag cttcgtctaa
      361 caaaaaagcc aaaaacggcc agaatttagc ggacaattta ctagtctaac actgaaaatt
      421 acatattgac ccaaatgatt acatttcaaa aggtgcctaa aaaacttcac aaaacacact
      481 cgccaacccc gagcgcatag ttcaaaaccg gagcttcagc tacttaagaa gataggtaca
      541 taaaaccgac caaagaaact gacgcctcac ttatccctcc cctcaccaga ggtccggcgc
      601 ctgtcgattc aggagagcct accctaggcc cgaaccctgc gtcctgcgac ggagaaaagc
      661 ctaccgcaca cctaccggca ggtggcccca ccctgcatta taagccaaca gaacgggtga
      721 cgtcacgaca cgacgagggc gcgcgctccc aaaggtacgg gtgcactgcc caacggcacc
      781 gccataactg ccgcccccgc aacagacgac aaaccgagtt ctccagtcag tgacaaactt
      841 cacgtcaggg tccccagatg gtgccccagc ccatctcacc cgaataagag ctttcccgca
      901 ttagcgaagg cctcaagacc ttgggttctt gccgcccacc atgcccccca ccttgtttca
      961 acgacctcac agcccgcctc acaagcgtct tccattcaag actcgggaac agccgccatt
     1021 ttgctgcgct ccccccaacc cccagttcag ggcaaccttg ctcgcggacc cagactacag
     1081 cccttggcgg tctctccaca cgcttccgtc ccaccgagcg gcccggcggc cacgaaagcc
     1141 ccggccagcc cagcagcccg ctactcacca agtgacgatc acagcgatcc acaaacaaga
     1201 accgcgaccc aaatcccggc tgcgacggaa ctagctgtgc cacacccggc gcgtccttat
     1261 ataatcatcg gcgttcaccg ccccacggag atccctccgc agaatcgccg agaagggact
     1321 acttttcctc gcctgttccg ctctctggaa agaaaaccag tgccctagag tcacccaagt
     1381 cccgtcctaa aatgtccttc tgctgatact ggggttctaa ggccgagtct tatgagcagc
     1441 gggccgctgt cctgagcgtc cgggcggaag gatcaggacg ctcgctgcgc ccttcgtctg
     1501 acgtggcagc gctcgccgtg aggagggggg cgcccgcggg aggcgccaaa acccggcgcg
     1561 gaggccagat ctggagccga cacgggttaa ttaaccaaac tggatctctg ctgtccctgt
     1621 aataaacccg aaaattttga atttttgtaa tttgtttttg taattcttta gtttgtatgt
     1681 ctgttgctat tatgtctact attctttccc ctgcactgta ccccccaatc cccccttttc
     1741 ttttaaaatt gtggatgaat actgccattt gtctgcagaa ttggcgcacg cagtgccgat
     1801 ccgttcacta atcgaatgga tctgtctctg tctctctctc caccttcttc ttctattcct
     1861 tcgggcctgt cgggtcccct cggggttggg aggtgggtct gaaacgataa tggtgaatat
     1921 ccctgcctaa ctctattcac tatagaaagt acagcaaaaa ctattcttaa acctaccaag
     1981 cctcctacta tcattatgaa taattttata taccacagcc aatttgttat gttaaaccaa
     2041 ttccacaaac ttgcccattt atctaattcc aataattctt gttcattctt ttcttgctgg
     2101 ttttgcgatt cttcaattaa ggagtgtatt aagcttgtgt aattgttaat ttctctgtcc
     2161 cactccatcc aggtcgtgtg attccaaatc tgttccagag atttattact ccaactagca
     2221 ttccaaggca cagcagtggt gcaaatgagt tttccagagc aaccccaaat ccccaggagc
     2281 tgttgatcct ttaggtatct ttccacagcc aggattcttg cctggagctg cttgatgccc
     2341 cagactgtga gttgcaacag atgctgttgc gcctcaatag ccctcagcaa attgttctgc
     2401 tgctgcacta taccagacaa taattgtctg gcctgtaccg tcagcgtcat tgacgctgcg
     2461 cccatagtgc ttcctgctgc tcccaagaac ccaaggaaca aagctcctat tcccactgct
     2521 cttttttctc tctgcaccac tcttctcttt gccttggtgg gtgctactcc taatggttca
     2581 atttttacta ctttatattt atataattca cttctccaat tgtccctcat atctcctcct
     2641 ccaggtctga agatcagcgg ccgcttgctg tgcggtggtc ttacttttgt tttgctcttc
     2701 ctctatcttg tctaaagctt ccttggtgtc ttttatctct atcctttgat gcacacaata
     2761 gagggttgct actgtattat ataatgatct aagttcttct gatcctgtct gaagggatgg
     2821 ttgtagctgt cccagtattt gtctacagcc ttctgatgtt tctaacaggc caggattaac
     2881 tgcgaatcgt tctagctccc tgcttgccca tactatatgt tttaatttat attttttctt
     2941 tccccctggc cttaaccgaa ttttttccca tcgcgatcta attctccccc gcttaatact
     3001 gacgctctcg cacccatctc tctccttcta gcctccgcta gtcaaaattt ttggcgtact
     3061 caccagtcgc cgcccctcgc ctcttgccgt gcgcgcttca gcaagccgag tcctgcgtcg
     3121 agagagctcc tctggtttcc ctttcgcttt caagtccctg ttcgggcgcc actgctagag
     3181 attttccaca ctgactaaaa gggtctgagg gatctctagt taccagagtc acacaacaga
     3241 cgggcacaca ctacttgaag cactcaaggc aagctttatt gaggcttaag cagtgggttc
     3301 cctagttagc cagagagctc ccaggctcag atctggtcta accagagaga cccagtacag
     3361 gcaaaacgcg ctgcttatat agacctccca ccgtacacgc ctaccgccca tttgcgtcaa
     3421 tggggcggag ttgttacgac attttggaaa gtcccgttga ttttggtgcc aaaacaaact
     3481 cccattgacg tcaatggggt ggagacttgg aaatccccgt gagtcaaacc gctatccacg
     3541 cccattgatg tactgccaaa accgcatcac catggtaata gcgatgacta atacgtagat
     3601 gtactgccaa gtaggaaagt cccataaggt catgtactgg gcataatgcc aggcgggcca
     3661 tttaccgtca ttgacgtcaa tagggggcgt acttggcata tgatacactt gatgtactgc
     3721 caagtgggca gtttaccgta aatactccac ccattgacgt caatggaaag tccctattgg
     3781 cgttactatg ggaacatacg tcattattga cgtcaatggg cgggggtcgt tgggcggtca
     3841 gccaggcggg ccatttaccg taagttatgt aacgcggaac tccatatatg ggctatgaac
     3901 taatgacccc gtaattgatt actattaata actagtcaat aatcaatgtc aacgcgtata
     3961 tctggcccgt acatcgcgaa gcagcgcaaa acgcctaacc ctaagcagat tcttcatgca
     4021 attgtcggtc aagccttgcc ttgttgtagc ttaaattttg ctcgcgcact actcagcgac
     4081 ctccaacaca caagcaggga gcagatactg gcttaactat gcggcatcag agcagattgt
     4141 actgagagtg caccataggg gatcgggaga tctcccgatc cgtcgacgtc aggtggcact
     4201 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     4261 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     4321 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     4381 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     4441 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     4501 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     4561 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     4621 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     4681 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     4741 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     4801 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     4861 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     4921 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     4981 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     5041 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     5101 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     5161 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     5221 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     5281 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     5341 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     5401 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     5461 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     5521 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     5581 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     5641 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     5701 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     5761 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     5821 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     5881 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     5941 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     6001 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     6061 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     6121 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
     6181 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
     6241 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
     6301 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccaagctc
     6361 tagctagagg tcgacggtat acagacatga taagatacat tgatgagttt ggacaaacca
     6421 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
     6481 ttgtaaccat tataagctgc aataaacaag ttggggtggg cgaagaactc cagcatgaga
     6541 tccccgcgct ggaggatcat ccagccggcg tcccggaaaa cgattccgaa gcccaacctt
     6601 tcatagaagg cggcggtgga atcgaaatct cgtagcacgt gtcagtcctg ctcctcggcc
     6661 acgaagtgca cgcagttgcc ggccgggtcg cgcagggcga actcccgccc ccacggctgc
     6721 tcgccgatct cggtcatggc cggcccggag gcgtcccgga agttcgtgga cacgacctcc
     6781 gaccactcgg cgtacagctc gtccaggccg cgcacccaca cccaggccag ggtgttgtcc
     6841 ggcaccacct ggtcctggac cgcgctgatg aacagggtca cgtcgtcccg gaccacaccg
     6901 gcgaagtcgt cctccacgaa gtcccgggag aacccgagcc ggtcggtcca gaactcgacc
     6961 gctccggcga cgtcgcgcgc ggtgagcacc ggaacggcac tggtcaactt ggccatggtt
     7021 tagttcctca ccttgtcgta ttatactatg ccgatatact atgccgatga ttaattgtca
     7081 acacgtgctg atcagatccg aaaatggata tacaagctcc cgggagcttt ttgcaaaagc
     7141 ctaggcctcc aaaaaagcct cctcactact tctggaatag ctcagaggca gaggcggcct
     7201 cggcctctgc ataaataaaa aaaattagtc agccatgggg cggagaatgg gcggaactgg
     7261 gcggagttag gggcgggatg ggcggagtta ggggcgggac tatggttgct gactaattga
     7321 gatgcatgct ttgcatactt ctgcctgctg gggagcctgg ggactttcca cacctggttg
     7381 ctgactaatt gagatgcatg ctttgcatac ttctgcctgc tggggagcct ggggactttc
     7441 cacaccctaa ctgacacaca ttccacagaa ttaattcgcg ttaaattttt gttaaatcag
     7501 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
     7561 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
     7621 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
     7681 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
     7741 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
     7801 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
     7861 caccacaccc gccgcgctta atgcgccgct acagggcgcg tggggatacc ccctagagcc
     7921 ccagctggtt ctttccgcct cagaagccat agagcccacc gcatccccag catgcctgct
     7981 attgtcttcc caatcctccc ccttgctgtc ctgccccacc ccacccccca gaatagaatg
     8041 acacctactc agacaatgcg atgcaatttc ctcattttat taggaaagga cagtgggagt
     8101 ggcaccttcc agggtcaagg aaggcacggg ggaggggcaa acaacagatg gctggcaact
     8161 agaaggcaca gtcgaggctg atcagcgggt ttaaacgggc cctgctagag attttccaca
     8221 ctgactaaaa gggtctgagg gatctctagt taccagagtc acacaacaga cgggcacaca
     8281 ctacttgaag cactcaaggc aagctttatt gaggcttaag cagtgggttc cctagttagc
     8341 cagagagctc ccaggctcag atctggtcta accagagaga cccagtacag tccggatgca
     8401 gctctcgggc catgtgatga aatgctaggc ggctgtcaaa cctccactct aatacttctc
     8461 tctccgggtc atccatccca tgcaggctca cagggtgtaa caagcgggtg ttctctcctt
     8521 cattggcttc ttctaccttc tcttgctcaa ctggtactag cttgtagcac catccaaagg
     8581 tcagtggata tctgatccct ggccctggtg tgtagttctg ccaatcaggg aagtagcctt
     8641 gtgtgtggta gatccacaga tcaaggatat cttgtcttcg ttgggagtga attagccctt
     8701 ccagtccccc cttttctttt aaaaagtggc taagatctac agctgccttg taagtcattg
     8761 gtcttaaagg tacctgaggt gtgactggaa aacccacctc ctcctcctct tgtgcttcta
     8821 gccaggcaca atcagcattg gtagctgctg tattgctact tgtgattgct ccatgttttt
     8881 ctaggtctcg aggtcgacgg tatcgatgcg gggaggcggc ccaaagggag atccgactcg
     8941 tctgagggcg aaggcgaaga cgcggaagag gccgcagagc cggcagcagg ccgcgggaag
     9001 gaaggtccgc tggattgagg gccgaaggga cgtagcagaa ggacgtcccg cgcagaatcc
     9061 aggtggcaac acaggcgagc agccaaggaa aggacgatga tttccccgac aacaccacgg
     9121 aattgtcagt gcccaacagc cgagcccctg tccagcagcg ggcaaggcag gcggcgatga
     9181 gttccgccgt ggcaataggg agggggaaag cgaaagtccc ggaaaggagc tgacaggtgg
     9241 tggcaatgcc ccaaccagtg ggggttgcgt cagcaaacac agtgcacacc acgccacgtt
     9301 gcctgacaac gggccacaac tcctcataaa gagacagcaa ccaggattta tacaaggagg
     9361 agaaaatgaa agccatacgg gaagcaatag catgatacaa aggcattaaa gcagcgtatc
     9421 cacatagcgt aaaaggagca acatagttaa gaataccagt caatctttca caaattttgt
     9481 aatccagagg ttgattatcg ataagcttga tatcgaattc tagagtcgcg gccgctttac
     9541 ttgtacagct cgtccatgcc gagagtgatc ccggcggcgg tcacgaactc cagcaggacc
     9601 atgtgatcgc gcttctcgtt ggggtctttg ctcagggcgg actgggtgct caggtagtgg
     9661 ttgtcgggca gcagcacggg gccgtcgccg atgggggtgt tctgctggta gtggtcggcg
     9721 agctgcacgc tgccgtcctc gatgttgtgg cggatcttga agttcacctt gatgccgttc
     9781 ttctgcttgt cggccatgat atagacgttg tggctgttgt agttgtactc cagcttgtgc
     9841 cccaggatgt tgccgtcctc cttgaagtcg atgcccttca gctcgatgcg gttcaccagg
     9901 gtgtcgccct cgaacttcac ctcggcgcgg gtcttgtagt tgccgtcgtc cttgaagaag
     9961 atggtgcgct cctggacgta gccttcgggc atggcggact tgaagaagtc gtgctgcttc
    10021 atgtggtcgg ggtagcggct gaagcactgc acgccgtagg tcagggtggt cacgagggtg
    10081 ggccagggca cgggcagctt gccggtggtg cagatgaact tcagggtcag cttgccgtag
    10141 gtggcatcg
//
