LOCUS       sequence-180583-       10253 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  lentiviral expression of EGFP and Ng shRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10253)
  AUTHORS   Hwang H, Szucs MJ, Ding LJ, Allen A, Ren X, Haensgen H, Gao F, Rhim 
            H, Andrade A, Pan JQ, Carr SA, Ahmad R, Xu W
  TITLE     Neurogranin, Encoded by the Schizophrenia Risk Gene NRGN, 
            Bidirectionally Modulates Synaptic Plasticity via 
            Calmodulin-Dependent Regulation of the Neuronal Phosphoproteome.
  JOURNAL   Biol Psychiatry. 2021 Feb 1;89(3):256-269. doi: 
            10.1016/j.biopsych.2020.07.014. Epub 2020 Jul 29.
  PUBMED    33032807
REFERENCE   2  (bases 1 to 10253)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.biopsych.2020.07.014"; journalName: "Biol Psychiatry"; 
            date: "2021-02-1- 1"; volume: "89"; issue: "3"; pages: "256-269"
COMMENT     Sequence Label: shNg Lenti FHRC3J1UGW
FEATURES             Location/Qualifiers
     source          1..10253
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     259..278
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             join(468..470,471..473,474..1187)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(513..534)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(774..793)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1121..1142
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    1230..1818
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1283..1303)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1701..1712)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2337..2517
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(2543..2560)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2549..2773
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      2819..3247
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2906..2925)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3116..3137
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3256..3276)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3261..3590
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3441..3576
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     3503..3522
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        3638..3685
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             3704..4078
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    4208..4329
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4245..4264)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4299..4318
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4378..4394)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4378..4394)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4391..4413)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4402..4418
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4426..4432,4433..4450,4451..4456))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4471..4492
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4609..4626)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4780..5368)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4860..4879)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5539..6330,6331..6399))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6162..6181
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6400..6504)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(6579..6598)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        6770..7149
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7151..7349
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     7304..7324
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             7367..7547
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7594..7719
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8212..8445
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8630..8674
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8972..9089
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        9142..9356
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     9277..9296
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     promoter        join(9464..10253,1..422)
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
ORIGIN
        1 ccgttgggca gtgcacccgt acctttggga gcgcgcgccc tcgtcgtgtc gtgacgtcac
       61 ccgttctgtt ggcttataat gcagggtggg gccacctgcc ggtaggtgtg cggtaggctt
      121 ttctccgtcg caggacgcag ggttcgggcc tagggtaggc tctcctgaat cgacaggcgc
      181 cggacctctg gtgaggggag ggataagtga ggcgtcagtt tctttggtcg gttttatgta
      241 cctatcttct taagtagctg aagctccggt tttgaactat gcgctcgggg ttggcgagtg
      301 tgttttgtga agttttttag gcaccttttg aaatgtaatc atttgggtca atatgtaatt
      361 ttcagtgtta gactagtaaa ttgtccgcta aattctggcc gtttttggct tttttgttag
      421 acgaagcttg ggctgcaggt cgactctaga ggatccaccg gtccaccatg gtgagcaagg
      481 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg
      541 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc
      601 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc
      661 tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct
      721 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg
      781 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg
      841 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca
      901 actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggcga
      961 acttcaagat ccgccacaac atcgaggacg gcggcgtgca gctcgccgac cactaccagc
     1021 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc
     1081 agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg
     1141 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtaagct agctcgagct
     1201 agaattaatt cgatatcaag cttatcgata atcaacctct ggattacaaa atttgtgaaa
     1261 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
     1321 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
     1381 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt
     1441 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc
     1501 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc
     1561 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg
     1621 ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga
     1681 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc
     1741 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc
     1801 tttgggccgc ctccccgcat cgataccgtc gagacctaga aaaacatgga gcaatcacaa
     1861 gtagcaatac agcagctacc aatgctgatt gtgcctggct agaagcacaa gaggaggagg
     1921 aggtgggttt tccagtcaca cctcaggtac ctttaagacc aatgacttac aaggcagctg
     1981 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac
     2041 gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc cctgattggc
     2101 agaactacac accagggcca gggatcagat atccactgac ctttggatgg tgctacaagc
     2161 tagtaccagt tgagcaagag aaggtagaag aagccaatga aggagagaac acccgcttgt
     2221 tacaccctgt gagcctgcat gggatggatg acccggagag agaagtatta gagtggaggt
     2281 ttgacagccg cctagcattt catcacatgg cccgagagct gcatccggac tgtactgggt
     2341 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     2401 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     2461 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcaggg
     2521 cccgtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt
     2581 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
     2641 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
     2701 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg
     2761 gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac
     2821 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     2881 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     2941 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     3001 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     3061 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     3121 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     3181 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     3241 gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
     3301 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
     3361 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
     3421 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     3481 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc
     3541 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg
     3601 gagcttgtat atccattttc ggatctgatc agcacgtgtt gacaattaat catcggcata
     3661 gtatatcggc atagtataat acgacaaggt gaggaactaa accatggcca agttgaccag
     3721 tgccgttccg gtgctcaccg cgcgcgacgt cgccggagcg gtcgagttct ggaccgaccg
     3781 gctcgggttc tcccgggact tcgtggagga cgacttcgcc ggtgtggtcc gggacgacgt
     3841 gaccctgttc atcagcgcgg tccaggacca ggtggtgccg gacaacaccc tggcctgggt
     3901 gtgggtgcgc ggcctggacg agctgtacgc cgagtggtcg gaggtcgtgt ccacgaactt
     3961 ccgggacgcc tccgggccgg ccatgaccga gatcggcgag cagccgtggg ggcgggagtt
     4021 cgccctgcgc gacccggccg gcaactgcgt gcacttcgtg gccgaggagc aggactgaca
     4081 cgtgctacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt
     4141 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc
     4201 ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     4261 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     4321 tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc
     4381 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     4441 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     4501 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     4561 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     4621 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     4681 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     4741 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     4801 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     4861 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     4921 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     4981 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     5041 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     5101 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     5161 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     5221 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     5281 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     5341 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     5401 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     5461 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     5521 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     5581 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     5641 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     5701 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     5761 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     5821 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     5881 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     5941 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     6001 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     6061 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     6121 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     6181 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     6241 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     6301 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     6361 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     6421 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     6481 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg
     6541 atcgggagat ctcccgatcc cctatggtgc actctcagta caatctgctc tgatgccgca
     6601 tagttaagcc agtatctgct ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc
     6661 aaaatttaag ctacaacaag gcaaggcttg accgacaatt gcatgaagaa tctgcttagg
     6721 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
     6781 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
     6841 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
     6901 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
     6961 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
     7021 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
     7081 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
     7141 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
     7201 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
     7261 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
     7321 cgtgtacggt gggaggtcta tataagcagc gcgttttgcc tgtactgggt ctctctggtt
     7381 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     7441 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     7501 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac
     7561 agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct
     7621 gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact
     7681 agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt
     7741 agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa
     7801 catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa
     7861 acatcagaag gctgtagaca aatactggga cagctacaac catcccttca gacaggatca
     7921 gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata
     7981 gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag
     8041 accaccgcac agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa
     8101 ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc
     8161 caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt
     8221 gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac
     8281 ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc
     8341 tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc
     8401 aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg
     8461 ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc
     8521 tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta
     8581 cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca
     8641 agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg
     8701 gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt
     8761 ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca
     8821 gacccacctc ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg
     8881 agagagagac agagacagat ccattcgatt agtgaacgga tcggcactgc gtgcgccaat
     8941 tctgcagaca aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt
     9001 acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac
     9061 aaaaacaaat tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccag
     9121 tttggttaat cgtacgaatt cgaacgctga cgtcatcaac ccgctccaag gaatcgcggg
     9181 cccagtgtca ctaggcggga acacccagcg cgcgtgcgcc ctggcaggaa gatggctgtg
     9241 agggacaggg gagtggcgcc ctgcaatatt tgcatgtcgc tatgtgttct gggaaatcac
     9301 cataaacgtg aaatgtcttt ggatttggga atcttataag ttctgtatga gaccacagat
     9361 gcccgtgaca agacttccct actgtttcaa gagaacagta gggaagtctt gtcacttttt
     9421 ggaaagctta tcgaattaat taacccgtgt cggctccaga tctggcctcc gcgccgggtt
     9481 ttggcgcctc ccgcgggcgc ccccctcctc acggcgagcg ctgccacgtc agacgaaggg
     9541 cgcagcgagc gtcctgatcc ttccgcccgg acgctcagga cagcggcccg ctgctcataa
     9601 gactcggcct tagaacccca gtatcagcag aaggacattt taggacggga cttgggtgac
     9661 tctagggcac tggttttctt tccagagagc ggaacaggcg aggaaaagta gtcccttctc
     9721 ggcgattctg cggagggatc tccgtggggc ggtgaacgcc gatgattata taaggacgcg
     9781 ccgggtgtgg cacagctagt tccgtcgcag ccgggatttg ggtcgcggtt cttgtttgtg
     9841 gatcgctgtg atcgtcactt ggtgagtagc gggctgctgg gctggccggg gctttcgtgg
     9901 ccgccgggcc gctcggtggg acggaagcgt gtggagagac cgccaagggc tgtagtctgg
     9961 gtccgcgagc aaggttgccc tgaactgggg gttgggggga gcgcagcaaa atggcggctg
    10021 ttcccgagtc ttgaatggaa gacgcttgtg aggcgggctg tgaggtcgtt gaaacaaggt
    10081 ggggggcatg gtgggcggca agaacccaag gtcttgaggc cttcgctaat gcgggaaagc
    10141 tcttattcgg gtgagatggg ctggggcacc atctggggac cctgacgtga agtttgtcac
    10201 tgactggaga actcggtttg tcgtctgttg cgggggcggc agttatggcg gtg
//
