LOCUS       sequence-180597-        8401 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentivirus destination vector for stable expression of 
            mCherry-tagged kinetochore marker CENP-A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8401)
  AUTHORS   Pemble H, Kumar P, van Haren J, Wittmann T
  TITLE     GSK3-mediated CLASP2 phosphorylation modulates kinetochore dynamics.
  JOURNAL   J Cell Sci. 2017 Apr 15;130(8):1404-1412. doi: 10.1242/jcs.194662. 
            Epub 2017 Feb 23.
  PUBMED    28232523
REFERENCE   2  (bases 1 to 8401)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1242/jcs.194662"; journalName: "J Cell Sci"; date: "2017-04-15- 
            15"; volume: "130"; issue: "8"; pages: "1404-1412"
COMMENT     Sequence Label: pLenti6-CENP-A-mCherry
FEATURES             Location/Qualifiers
     source          1..8401
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(98..122)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(218..242)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(239..442)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(268..288)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        443..746
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             complement(1091..1135)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1320..1553
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2046..2171
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             2218..2398
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(2399..2625)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(2653..2673)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(2653..2671)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2692..2708)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2692..2708)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2705..2727)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2716..2732
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2740..2746,2747..2764,2765..2770))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2785..2806
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2923..2940)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3094..3682)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3174..3193)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3853..4644,4645..4713))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4476..4495
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4714..4818)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(4844..5299)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4981..5002)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5193..5212
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5426..5448
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5440..5457
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5441..5457
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5467..5486
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5467..5485
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      5506..5641
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    5681..5802
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5692..5711)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5746..5765
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             5874..6107
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     CDS             complement(6194..6592)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     promoter        complement(6611..6658)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(6706..7035)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6720..6855
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7020..7040
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     CDS             complement(7077..7118)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    7171..7195
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(7216..7926)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(7494..7513)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     7763..7781
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      7923..7932
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(7957..8046)
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
ORIGIN
        1 gggccggggg tcggggtcgg gctcgggctg cgcctcctcg gggcctcggg ctttcggctc
       61 cggcggcgcg ggcccatggt gaagggggcg gccgcggagc ctgctttttt gtacaaactt
      121 gttgatatct gcagaattcc accacactgg actagtggat cctctagagt cggtgtcttc
      181 tatggaggtc aaaacagcgt ggatggcgtc tccaggcgat ctgacggttc actaaacgag
      241 ctctgcttat atagacctcc caccgtacac gcctaccgcc catttgcgtc aatggggcgg
      301 agttgttacg acattttgga aagtcccgtt gattttggtg ccaaaacaaa ctcccattga
      361 cgtcaatggg gtggagactt ggaaatcccc gtgagtcaaa ccgctatcca cgcccattga
      421 tgtactgcca aaaccgcatc accatggtaa tagcgatgac taatacgtag atgtactgcc
      481 aagtaggaaa gtcccataag gtcatgtact gggcataatg ccaggcgggc catttaccgt
      541 cattgacgtc aatagggggc gtacttggca tatgatacac ttgatgtact gccaagtggg
      601 cagtttaccg taaatactcc acccattgac gtcaatggaa agtccctatt ggcgttacta
      661 tgggaacata cgtcattatt gacgtcaatg ggcgggggtc gttgggcggt cagccaggcg
      721 ggccatttac cgtaagttat gtaacgcgga actcccaagc ttatcgcaga cccagtcgct
      781 agcgttaacg tcgacatcgc agacccagtc gctagcgtta acgtcgacat cgataccgtc
      841 gagatccgtt cactaatcga atggatctgt ctctgtctct ctctccacct tcttcttcta
      901 ttccttcggg cctgtcgggt cccctcgggg ttgggaggtg ggtctgaaac gataatggtg
      961 aatatccctg cctaactcta ttcactatag aaagtacagc aaaaactatt cttaaaccta
     1021 ccaagcctcc tactatcatt atgaataatt ttatatacca cagccaattt gttatgttaa
     1081 accaattcca caaacttgcc catttatcta attccaataa ttcttgttca ttcttttctt
     1141 gctggttttg cgattcttca attaaggagt gtattaagct tgtgtaattg ttaatttctc
     1201 tgtcccactc catccaggtc gtgtgattcc aaatctgttc cagagattta ttactccaac
     1261 tagcattcca aggcacagca gtggtgcaaa tgagttttcc agagcaaccc caaatcccca
     1321 ggagctgttg atcctttagg tatctttcca cagccaggat tcttgcctgg agctgcttga
     1381 tgccccagac tgtgagttgc aacagatgct gttgcgcctc aatagccctc agcaaattgt
     1441 tctgctgctg cactatacca gacaataatt gtctggcctg taccgtcagc gtcattgacg
     1501 ctgcgcccat agtgcttcct gctgctccca agaacccaag gaacaaagct cctattccca
     1561 ctgctctttt ttctctctgc accactcttc tctttgcctt ggtgggtgct actcctaatg
     1621 gttcaatttt tactacttta tatttatata attcacttct ccaattgtcc ctcatatctc
     1681 ctcctccagg tctgaagatc agcggccgct tgctgtgcgg tggtcttact tttgttttgc
     1741 tcttcctcta tcttgtctaa agcttccttg gtgtctttta tctctatcct ttgatgcaca
     1801 caatagaggg ttgctactgt attatataat gatctaagtt cttctgatcc tgtctgaagg
     1861 gatggttgta gctgtcccag tatttgtcta cagccttctg atgtttctaa caggccagga
     1921 ttaactgcga atcgttctag ctccctgctt gcccatacta tatgttttaa tttatatttt
     1981 ttctttcccc ctggccttaa ccgaattttt tcccatcgcg atctaattct cccccgctta
     2041 atactgacgc tctcgcaccc atctctctcc ttctagcctc cgctagtcaa aatttttggc
     2101 gtactcacca gtcgccgccc ctcgcctctt gccgtgcgcg cttcagcaag ccgagtcctg
     2161 cgtcgagaga gctcctctgg tttccctttc gctttcaagt ccctgttcgg gcgccactgc
     2221 tagagatttt ccacactgac taaaagggtc tgagggatct ctagttacca gagtcacaca
     2281 acagacgggc acacactact tgaagcactc aaggcaagct ttattgaggc ttaagcagtg
     2341 ggttccctag ttagccagag agctcccagg ctcagatctg gtctaaccag agagacccgt
     2401 ttatgtatcg agctaggcac ttaaatacaa tatctctgca atgcggcaat tcagtggttc
     2461 gtccaatcca tgtcagaccc gtctgttgcc ttcctaataa ggcacgatcg taccacctta
     2521 cttccaccaa tcggcatgca cggtgctttt tctctccttg taaggcatgt tgctaactca
     2581 tcgttaccat gttgcaagac tacaagagta ttgcataaga ctacattaag cttgcagctc
     2641 cagcttttgt tccctttagt gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct
     2701 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
     2761 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
     2821 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     2881 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     2941 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     3001 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     3061 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     3121 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     3181 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     3241 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     3301 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     3361 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     3421 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     3481 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     3541 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     3601 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     3661 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     3721 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     3781 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     3841 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     3901 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     3961 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     4021 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     4081 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     4141 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     4201 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     4261 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     4321 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     4381 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     4441 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     4501 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     4561 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     4621 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     4681 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     4741 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     4801 acaaataggg gttccgcgca catttccccg aaaagtgcca cctaaattgt aagcgttaat
     4861 attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc
     4921 gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt
     4981 ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa
     5041 accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg
     5101 tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga
     5161 cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct
     5221 agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat
     5281 gcgccgctac agggcgcgtc ccattcgcca ttcaggctgc gcaactgttg ggaagggcga
     5341 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
     5401 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgag
     5461 cgcgcgtaat acgactcact atagggcgaa ttgggtacgt ccctaggcct ccaaaaaagc
     5521 ctcctcacta cttctggaat agctcagagg ccgaggcggc ctcggcctct gcataaataa
     5581 aaaaaattag tcagccatgg ggcggagaat gggcggaact gggcggagtt aggggcggga
     5641 tgggcggagt taggggcggg atagctagag ccagacatga taagatacat tgatgagttt
     5701 ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct
     5761 attgctttat ttgtaaccat tataagctgc aataaacaag ttcctctcac tctctgatat
     5821 tcatttcttt gcaagttata aatactgaat aataagatga catgaactac tactgctaga
     5881 gattttccac actgactaaa agggtctgag ggatctctag ttaccagagt cacacaacag
     5941 acgggcacac actacttgaa gcactcaagg caagctttat tgaggcttaa gcagtgggtt
     6001 ccctagttag ccagagagct cccaggctca gatctggtct aaccagagag acccagtaca
     6061 agcaaaaagc agatcttgtc ttcgttggga gtgaattagc ccttccagtc cccccttttc
     6121 ttttaaaaag tggctaagat ctacagctgc cttgtaagtc attggtctta aaggtaccga
     6181 gctcgaattg tgcttagccc tcccacacat aaccagaggg cagcaattca cgaatcccaa
     6241 ctgccgtcgg ctgtccatca ctgtccttca ctatggcttt gatcccagga tgcagatcga
     6301 gaagcacctg tcggcaccgt ccgcaggggc tcaagatgcc cctgttctca tttccgatcg
     6361 cgacgataca agtcaggttg ccagctgccg cagcagcagc agtgcccagc accacgagtt
     6421 ctgcacaagg tcccccagta aaatgatata cattgacacc agtgaagatg cggccgtcgc
     6481 tagagagagc tgcgctggcg acgctgtagt cttcagagat ggggatgctg ttgattgtag
     6541 ccgttgctct ttcaatgagg gtggattctt cttgagacaa aggcttggcc atggtttagt
     6601 tcctcacctt gtcgtattat actatgccga tatactatgc cgatgattaa ttgtcaacac
     6661 gtgctgatca gatccgaaaa tggatataca agctcccggg agctttttgc aaaagcctag
     6721 gcctccaaaa aagcctcctc actacttctg gaatagctca gaggcagagg cggcctcggc
     6781 ctctgcataa ataaaaaaaa ttagtcagcc atggggcgga gaatgggcgg aactgggcgg
     6841 agttaggggc gggatgggcg gagttagggg cgggactatg gttgctgact aattgagatg
     6901 catgctttgc atacttctgc ctgctgggga gcctggggac tttccacacc tggttgctga
     6961 ctaattgaga tgcatgcttt gcatacttct gcctgctggg gagcctgggg actttccaca
     7021 ccctaactga cacacattcc acagaattaa ttccaaactc attactaacc ggtacgcgta
     7081 gaatcgagac cgaggagagg gttagggata ggcttacctt cgaaccgcgg gccctctaga
     7141 ctcgagcggc cgccactgtg ctggatatca accactttgt acaagaaagc tgggtcggcg
     7201 cgcccaccct tgcgcttact tgtacagctc gtccatgccg ccggtggagt ggcggccctc
     7261 ggcgcgttcg tactgttcca cgatggtgta gtcctcgttg tgggaggtga tgtccaactt
     7321 gatgttgacg ttgtaggcgc cgggcagctg cacgggcttc ttggccttgt aggtggtctt
     7381 gacctcagcg tcgtagtggc cgccgtcctt cagcttcagc ctctgcttga tctcgccctt
     7441 cagggcgccg tcctcggggt acatccgctc ggaggaggcc tcccagccca tggtcttctt
     7501 ctgcattacg gggccgtcgg aggggaagtt ggtgccgcgc agcttcacct tgtagatgaa
     7561 ctcgccgtcc tgcagggagg agtcctgggt cacggtcacc acgccgccgt cctcgaagtt
     7621 catcacgcgc tcccacttga agccctcggg gaaggacagc ttcaagtagt cggggatgtc
     7681 ggcggggtgc ttcacgtagg ccttggagcc gtacatgaac tgaggggaca ggatgtccca
     7741 ggcgaagggc agggggccac ccttggtcac cttcagcttg gcggtctggg tgccctcgta
     7801 ggggcggccc tcgccctcgc cctcgatctc gaactcgtgg ccgttcacgg agccctccat
     7861 gtgcaccttg aagcgcatga actccttgat gatggccatg ttatcctcct cgcccttgct
     7921 caccatggtg gcgaccggtg cgcggccgca ctgagcagcg taatctggaa cgtcatatgg
     7981 ataggatcct gcatagtccg ggacgtcata gggatagccc gcatagtcag gaacatcgta
     8041 tgggtaaaag atgcggccgc cgagtccctc ctcaaggccc cggatcctcc gggccagttg
     8101 cacatccttt gggaagagag taactcggcc tgcatgtaag gtgaggagat aggcgtcctc
     8161 aaagagatga actagaaatg cttctgctgc ctcttgtagg gccaataggg cctgggcttg
     8221 ccaattgaag tccacaccac gagtgaattt aacacatatt tctcttgcca ggcggctgaa
     8281 gggcagcttc cttatcaaga ggtgtgtgct cttctgaagc tttcggatct cctttagcca
     8341 accttgtctc cgccgactgt gttgatggga ggaagcgcct aaggaggggc cccgccggga
     8401 g
//
