LOCUS       sequence-180605-        7556 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Human ZMPSTE24 mammalian expression vector. Retroviral bicistronic 
            puromycin vector..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7556)
  AUTHORS   Fu B, Wang L, Li S, Dorf ME
  TITLE     ZMPSTE24 defends against influenza and other pathogenic viruses.
  JOURNAL   J Exp Med. 2017 Feb 28. pii: jem.20161270. doi: 
            10.1084/jem.20161270.
  PUBMED    28246125
REFERENCE   2  (bases 1 to 7556)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp Med. 
            2017 Feb 28. pii: jem.20161270. doi: 10.1084/jem.20161270."
COMMENT     Sequence Label: hZMPSTE24-3FLAG_pQCXIP
FEATURES             Location/Qualifiers
     source          1..7556
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        27..330
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        331..534
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     531..555
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      679..688
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2131..2202
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags"
                     /label=3xFLAG
                     /translation="DYKDDDDKDYKDDDDKDYKDDDDK"
     LTR             2685..3110
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     Moloney murine leukemia virus"
     primer_bind     complement(3213..3235)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3371..3391)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3376..3705
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3556..3691
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     3727..3746
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(3827..3844)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3998..4586)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4078..4097)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4757..5548,5549..5617))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5380..5399
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5618..5722)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5790..5808
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     enhancer        5908..6287
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6288..6491
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             6492..6667
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    6730..6929
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             7130..7546
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     7476..7498
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     7514..7530
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
ORIGIN
        1 atctggggga tcgatcctct agagtccgtt acataactta cggtaaatgg cccgcctggc
       61 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
      121 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
      181 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
      241 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
      301 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
      361 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
      421 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
      481 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta
      541 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac
      601 cgggaccgat ccagcctccg cggccgggaa cggtgcattg gaacggacct gcaggaattg
      661 atccgcggcc gcaccggtgc caccatgggg atgtgggcat cgctggacgc tttgtgggag
      721 atgccggccg agaagcgtat cttcggggcc gtgctgctct tttcctggac agtgtatctt
      781 tgggagacct tcctagcaca gcggcagaga aggatatata aaacaacaac tcatgtacca
      841 ccggagttag gacagatcat ggattctgaa acatttgaga aatctcgact ctatcaactg
      901 gataaaagca ctttcagctt ctggtcagga ctctattcag agactgaagg cactcttatt
      961 cttctctttg gaggaatacc ttatctctgg agactttctg gacggttctg tggttatgct
     1021 ggctttggac cagaatatga gatcactcag tccctggtgt ttctgctgtt ggctacactt
     1081 ttcagtgcat tggctggttt gccatggagt ctttataata cttttgtgat agaagaaaaa
     1141 catggcttca atcaacagac tttggggttc ttcatgaaag atgcaatcaa gaaatttgtt
     1201 gtgactcagt gcattttgtt gcctgtgtct tcacttctac tttacattat taaaattggg
     1261 ggtgactatt tttttattta tgcctggctg ttcacattag ttgtgtctct ggttcttgtc
     1321 acaatctatg ctgattatat tgccccttta tttgacaaat tcacacctct gcctgaggga
     1381 aagcttaaag aagaaattga agtaatggca aagagtattg actttccttt gacgaaggtg
     1441 catgttgtgg aaggatctaa acgctcttcc cacagcaatg cttattttta tggcttcttc
     1501 aagaacaagc gaatagtttt gtttgacact ctactagaag agtactctgt actaaacaaa
     1561 gacatccagg aggattctgg catggaaccc cgcaatgagg aagaagggaa cagtgaagaa
     1621 ataaaagcta aagttaaaaa taagaaacaa ggatgtaaaa atgaggaggt actcgctgta
     1681 ctaggccatg aactggggca ctggaagttg ggacatacag tcaaaaatat cattattagc
     1741 cagatgaatt ctttcctgtg ttttttttta tttgctgtat taattggtcg aaaggagctt
     1801 tttgctgcat ttggttttta tgatagccaa cccactctta ttggactatt gatcatcttc
     1861 cagtttattt tttcacctta caatgaggtt ctttcttttt gcctaacagt cctaagccgc
     1921 agatttgagt ttcaagctga tgcatttgcc aagaaacttg ggaaggctaa agacttatat
     1981 tctgctttaa tcaaacttaa caaagataac ttgggattcc ctgtttctga ctggttgttc
     2041 tcaatgtggc attattctca tcctccactg ctagagagac ttcaagcttt gaaaactatg
     2101 aagcaacacg cggccgcaat cgatgtcgag gattacaagg atgacgacga taaggactat
     2161 aaggacgatg atgacaagga ctacaaagat gatgacgata aatagggaca acctcccctt
     2221 ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac
     2281 ctggtgcatg acccgcaagc ccggtgcctg acgcccgccc cacgacccgc agcgcccgac
     2341 cgaaaggagc gcacgacccc atggctccga ccgaagccac ccggggcggc cccgccgacc
     2401 ccgcacccgc ccccgaggcc caccgactct agtcgagggc tgcagcgctg cagaggccga
     2461 gtgcagaact gctccaaagg gacctcaagg ctttccgagg gacactaggc tgactccatc
     2521 gagccagtgt agagataagc ttatcgatta gtccaatttg ttaaagacag gatatcagtg
     2581 gtccaggctc tagttttgac tcaacaatat caccagctga agcctataga gtacgagcca
     2641 tagataaaat aaaagatttt atttagtctc cagaaaaagg ggggaatgaa agaccccacc
     2701 tgtaggtttg gcaagctagc ttaagtaacg ccattttgca aggcatggaa aaatacataa
     2761 ctgagaatag agaagttcag atcaaggtca ggaacagatg gaacagggtc gaccctagag
     2821 aaccatcaga tgtttccagg gtgccccaag gacctgaaat gaccctgtgc cttatttgaa
     2881 ctaaccaatc agttcgcttc tcgcttctgt tcgcgcgctt ctgctccccg agctcaataa
     2941 aagagcccac aacccctcac tcggggcgcc agtcctccga ttgactgagt cgcccgggta
     3001 cccgtgtatc caataaaccc tcttgcagtt gcatccgact tgtggtctcg ctgttccttg
     3061 ggagggtctc ctctgagtga ttgactaccc gtcagcgggg gtctttcatt tgggggctcg
     3121 tccgggatcg ggagacccct gcccagggac caccgaccca ccaccgggag gtaagctggc
     3181 tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg
     3241 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg
     3301 ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtag
     3361 atccggctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc
     3421 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
     3481 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg
     3541 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     3601 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
     3661 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct tactggctta
     3721 actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc
     3781 acagatgcgt aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg ctcactgact
     3841 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     3901 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     3961 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     4021 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     4081 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     4141 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     4201 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     4261 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     4321 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     4381 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga
     4441 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     4501 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     4561 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     4621 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     4681 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     4741 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     4801 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     4861 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     4921 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     4981 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     5041 ttaatagttt gcgcaacgtt gttgccattg ctgcaggcat cgtggtgtca cgctcgtcgt
     5101 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     5161 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     5221 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     5281 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     5341 tgcggcgacc gagttgctct tgcccggcgt caacacggga taataccgcg ccacatagca
     5401 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     5461 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     5521 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     5581 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     5641 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     5701 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa
     5761 ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtcttc
     5821 aagaattagc ttggccattg catacgttgt atccatatca taatatgtac atttatattg
     5881 gctcatgtcc aacattaccg ccatgttgac attgattatt gactagttat taatagtaat
     5941 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg
     6001 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
     6061 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
     6121 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
     6181 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
     6241 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
     6301 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
     6361 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
     6421 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
     6481 taagcagagc tcaataaaag agcccacaac ccctcactcg gcgcgccagt cttccgatag
     6541 actgcgtcgc ccgggtaccc gtattcccaa taaagcctct tgctgtttgc atccgaatcg
     6601 tggtctcgct gttccttggg agggtctcct ctgagtgatt gactacccac gacgggggtc
     6661 tttcatttgg gggctcgtcc gggatttgga gacccctgcc cagggaccac cgacccacca
     6721 ccgggaggta agctggccag caacttatct gtgtctgtcc gattgtctag tgtctatgtt
     6781 tgatgttatg cgcctgcgtc tgtactagtt agctaactag ctctgtatct ggcggacccg
     6841 tggtggaact gacgagttct gaacacccgg ccgcaaccct gggagacgtc ccagggactt
     6901 tgggggccgt ttttgtggcc cgacctgagg aagggagtcg atgtggaatc cgaccccgtc
     6961 aggatatgtg gttctggtag gagacgagaa cctaaaacag ttcccgcctc cgtctgaatt
     7021 tttgctttcg gtttggaacc gaagccgcgc gtcttgtctg ctgcagcgct gcagcatcgt
     7081 tctgtgttgt ctctgtctga ctgtgtttct gtatttgtct gaaaattagg gccagactgt
     7141 taccactccc ttaagtttga ccttaggtca ctggaaagat gtcgagcgga tcgctcacaa
     7201 ccagtcggta gatgtcaaga agagacgttg ggttaccttc tgctctgcag aatggccaac
     7261 ctttaacgtc ggatggccgc gagacggcac ctttaaccga gacctcatca cccaggttaa
     7321 gatcaaggtc ttttcacctg gcccgcatgg acacccagac caggtcccct acatcgtgac
     7381 ctgggaagcc ttggcttttg acccccctcc ctgggtcaag ccctttgtac accctaagcc
     7441 tccgcctcct cttcctccat ccgccccgtc tctccccctt gaacctcctc gttcgacccc
     7501 gcctcgatcc tccctttatc cagccctcac tccttctcta ggcgccggaa ttgaag
//
