LOCUS       sequence-180615-        8487 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive expression of creGFP fusion using PGK promoter in a 
            lentiviral backbone..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8487)
  TITLE     Torok-Storb lab lentiviral fluorescent protein
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8487)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pRRlsinPGK_CREGFP_WPRE
FEATURES             Location/Qualifiers
     source          1..8487
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     238..257
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     regulatory      560..569
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(579..1607)
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MANLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     regulatory      1604..1613
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(1634..2144)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(1697..1715)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     misc_feature    2190..2307
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(2574..2618)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2803..3036
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    3529..3654
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             3701..3881
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(3882..4108)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(4136..4156)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(4136..4154)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4175..4191)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4175..4191)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4188..4210)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4199..4215
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4223..4229,4230..4247,4248..4253))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     primer_bind     complement(4406..4423)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4577..5165)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4657..4676)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5336..6127,6128..6196))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5959..5978
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6197..6301)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(6327..6782)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6464..6485)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6676..6695
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6909..6931
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6923..6940
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6924..6940
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6950..6969
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6950..6968
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      6989..7124
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    7164..7285
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7175..7194)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7229..7248
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             7357..7590
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    7677..8265
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             7783..7794
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     8192..8212
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(join(8331..8487,1..557,558..560,561..563))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(8334..8487,1..557,558..560,561..563))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(8376..8397)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ggggtgttct gctggtagtg gtcggcgagc tgcacgctgc cgtcctcgat gttgtggcgg
       61 atcttgaagt tcaccttgat gccgttcttc tgcttgtcgg ccatgatata gacgttgtgg
      121 ctgttgtagt tgtactccag cttgtgcccc aggatgttgc cgtcctcctt gaagtcgatg
      181 cccttcagct cgatgcggtt caccagggtg tcgccctcga acttcacctc ggcgcgggtc
      241 ttgtagttgc cgtcgtcctt gaagaagatg gtgcgctcct ggacgtagcc ttcgggcatg
      301 gcggacttga agaagtcgtg ctgcttcatg tggtcggggt agcggctgaa gcactgcacg
      361 ccgtaggtca gggtggtcac gagggtgggc cagggcacgg gcagcttgcc ggtggtgcag
      421 atgaacttca gggtcagctt gccgtaggtg gcatcgccct cgccctcgcc ggacacgctg
      481 aacttgtggc cgtttacgtc gccgtccagc tcgaccagga tgggcaccac cccggtgaac
      541 agctcctcgc ccttgctcac catggtggcg accggtccat cgccatcttc cagcaggcgc
      601 accattgccc ctgtttcact atccaggtta cggatatagt tcatgacaat atttacattg
      661 gtccagccac cagcttgcat gatctccggt attgaaactc cagcgcgggc catatctcgc
      721 gcggctccga cacgggcact gtgtccagac caggccaggt atctctgacc agagtcatcc
      781 ttagcgccgt aaatcaatcg atgagttgct tcaaaaatcc cttccagggc gcgagttgat
      841 agctggctgg tggcagatgg cgcggcaaca ccattttttc tgacccggca aaacaggtag
      901 ttattcggat catcagctac accagagacg gaaatccatc gctcgaccag tttagttacc
      961 cccaggctaa gtgccttctc tacacctgcg gtgctaacca gcgttttcgt tctgccaata
     1021 tggattaaca ttctcccacc gtcagtacgt gagatatctt taaccctgat cctggcaatt
     1081 tcggctatac gtaacagggt gttataagca atccccagaa atgccagatt acgtatatcc
     1141 tggcagcgat cgctattttc catgagtgaa cgaacctggt cgaaatcagt gcgttcgaac
     1201 gctagagcct gttttgcacg ttcaccggca tcaacgtttt cttttcggat ccgccgcata
     1261 accagtgaaa cagcattgct gtcacttggt cgtggcagcc cggaccgacg atgaagcatg
     1321 tttagctggc ccaaatgttg ctggatagtt tttactgcca gaccgcgcgc ctgaagatat
     1381 agaagataat cgcgaacatc ttcaggttct gcgggaaacc atttccggtt attcaacttg
     1441 caccatgccg cccacgaccg gcaaacggac agaagcattt tccaggtatg ctcagaaaac
     1501 gcctggcgat ccctgaacat gtccatcagg ttcttgcgaa cctcatcact cgttgcatcg
     1561 accggtaatg caggcaaatt ttggtgtacg gtcagtaaat tggccatggt ggcggctcag
     1621 aattctttgc cccctgggga gagaggtcgg tgattcggtc aacgagggag ccgactgccg
     1681 acgtgcgctc cggaggcttg cagaatgcgg aacaccgcgc gggcaggaac agggcccaca
     1741 ctaccgcccc acaccccgcc tcccgcaccg ccccttcccg gccgctgctc tcggcgcgcc
     1801 ctgctgagca gccgctattg gccacagccc atcgcggtcg gcgcgctgcc attgctccct
     1861 ggcgctgtcc gtctgcgagg gtactagtga gacgtgcggc ttccgtttgt cacgtccggc
     1921 acgccgcgaa ccgcaaggaa ccttcccgac ttaggggcgg agcaggaagc gtcgccgggg
     1981 ggcccacaag ggtagcggcg aagatccggg tgacgctgcg aacggacgtg aagaatgtgc
     2041 gagacccagg gtcggcgccg ctgcgtttcc cggaaccacg cccagagcag ccgcgtccct
     2101 gcgcaaaccc agggctgcct tggaaaaggc gcaaccccaa ccccgtggaa ttcgatatca
     2161 agcttctcga ggctagtctc gtgatcgata aaattttgaa tttttgtaat ttgtttttgt
     2221 aattctttag tttgtatgtc tgttgctatt atgtctacta ttctttcccc tgcactgtac
     2281 cccccaatcc ccccttttct tttaaaagtt aaccgatacc gtcgagatcc gttcactaat
     2341 cgaatggatc tgtctctgtc tctctctcca ccttcttctt ctattccttc gggcctgtcg
     2401 ggtcccctcg gggttgggag gtgggtctga aacgataatg gtgaatatcc ctgcctaact
     2461 ctattcacta tagaaagtac agcaaaaact attcttaaac ctaccaagcc tcctactatc
     2521 attatgaata attttatata ccacagccaa tttgttatgt taaaccaatt ccacaaactt
     2581 gcccatttat ctaattccaa taattcttgt tcattctttt cttgctggtt ttgcgattct
     2641 tcaattaagg agtgtattaa gcttgtgtaa ttgttaattt ctctgtccca ctccatccag
     2701 gtcgtgtgat tccaaatctg ttccagagat ttattactcc aactagcatt ccaaggcaca
     2761 gcagtggtgc aaatgagttt tccagagcaa ccccaaatcc ccaggagctg ttgatccttt
     2821 aggtatcttt ccacagccag gattcttgcc tggagctgct tgatgcccca gactgtgagt
     2881 tgcaacagat gctgttgcgc ctcaatagcc ctcagcaaat tgttctgctg ctgcactata
     2941 ccagacaata attgtctggc ctgtaccgtc agcgtcattg acgctgcgcc catagtgctt
     3001 cctgctgctc ccaagaaccc aaggaacaaa gctcctattc ccactgctct tttttctctc
     3061 tgcaccactc ttctctttgc cttggtgggt gctactccta atggttcaat ttttactact
     3121 ttatatttat ataattcact tctccaattg tccctcatat ctcctcctcc aggtctgaag
     3181 atcagcggcc gcttgctgtg cggtggtctt acttttgttt tgctcttcct ctatcttgtc
     3241 taaagcttcc ttggtgtctt ttatctctat cctttgatgc acacaataga gggttgctac
     3301 tgtattatat aatgatctaa gttcttctga tcctgtctga agggatggtt gtagctgtcc
     3361 cagtatttgt ctacagcctt ctgatgtttc taacaggcca ggattaactg cgaatcgttc
     3421 tagctccctg cttgcccata ctatatgttt taatttatat tttttctttc cccctggcct
     3481 taaccgaatt ttttcccatc gcgatctaat tctcccccgc ttaatactga cgctctcgca
     3541 cccatctctc tccttctagc ctccgctagt caaaattttt ggcgtactca ccagtcgccg
     3601 cccctcgcct cttgccgtgc gcgcttcagc aagccgagtc ctgcgtcgag agagctcctc
     3661 tggtttccct ttcgctttca agtccctgtt cgggcgccac tgctagagat tttccacact
     3721 gactaaaagg gtctgaggga tctctagtta ccagagtcac acaacagacg ggcacacact
     3781 acttgaagca ctcaaggcaa gctttattga ggcttaagca gtgggttccc tagttagcca
     3841 gagagctccc aggctcagat ctggtctaac cagagagacc cgtttatgta tcgagctagg
     3901 cacttaaata caatatctct gcaatgcggc aattcagtgg ttcgtccaat ccatgtcaga
     3961 cccgtctgtt gccttcctaa taaggcacga tcgtaccacc ttacttccac caatcggcat
     4021 gcacggtgct ttttctctcc ttgtaaggca tgttgctaac tcatcgttac catgttgcaa
     4081 gactacaaga gtattgcata agactacatt aagcttgcag ctccagcttt tgttcccttt
     4141 agtgagggtt aattgcgcgc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
     4201 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     4261 gtgcctaatg agtgagctaa ctcaccttaa ttgcgttgcg ctcactgccc gctttccagt
     4321 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     4381 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     4441 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     4501 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     4561 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     4621 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     4681 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     4741 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     4801 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     4861 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     4921 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     4981 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     5041 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     5101 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     5161 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     5221 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     5281 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     5341 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     5401 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     5461 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     5521 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     5581 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     5641 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     5701 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     5761 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     5821 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     5881 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     5941 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     6001 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     6061 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     6121 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     6181 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     6241 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     6301 gcacatttcc ccgaaaagtg ccacctaaat tgtaagcgtt aatattttgt taaaattcgc
     6361 gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc
     6421 ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag
     6481 tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga
     6541 tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc
     6601 actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa
     6661 cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt
     6721 agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc
     6781 gtcccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc
     6841 gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc
     6901 agggttttcc cagtcacgac gttgtaaaac gacggccagt gagcgcgcgt aatacgactc
     6961 actatagggc gaattgggta cgtccctagg cctccaaaaa agcctcctca ctacttctgg
     7021 aatagctcag aggccgaggc ggcctcggcc tctgcataaa taaaaaaaat tagtcagcca
     7081 tggggcggag aatgggcgga actgggcgga gttaggggcg ggatgggcgg agttaggggc
     7141 gggatagcta gagccagaca tgataagata cattgatgag tttggacaaa ccacaactag
     7201 aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac
     7261 cattataagc tgcaataaac aagttcctct cactctctga tattcatttc tttgcaagtt
     7321 ataaatactg aataataaga tgacatgaac tactactgct agagattttc cacactgact
     7381 aaaagggtct gagggatctc tagttaccag agtcacacaa cagacgggca cacactactt
     7441 gaagcactca aggcaagctt tattgaggct taagcagtgg gttccctagt tagccagaga
     7501 gctcccaggc tcagatctgg tctaaccaga gagacccagt acaagcaaaa agcagatctt
     7561 gtcttcgttg ggagtgaatt agcccttcca gtcccccctt ttcttttaaa aagtggctaa
     7621 gatctacagc tgccttgtaa gtcattggtc ttaaaggtac cgagctcgaa ttccaggcgg
     7681 ggaggcggcc caaagggaga tccgactcgt ctgagggcga aggcgaagac gcggaagagg
     7741 ccgcagagcc ggcagcaggc cgcgggaagg aaggtccgct ggattgaggg ccgaagggac
     7801 gtagcagaag gacgtcccgc gcagaatcca ggtggcaaca caggcgagca gccatggaaa
     7861 ggacgtcagc ttccccgaca acaccacgga attgtcagtg cccaacagcc gagcccctgt
     7921 ccagcagcgg gcaaggcagg cggcgatgag ttccgccgtg gcaataggga gggggaaagc
     7981 gaaagtcccg gaaaggagct gacaggtggt ggcaatgccc caaccagtgg gggttgcgtc
     8041 agcaaacaca gtgcacacca cgccacgttg cctgacaacg ggccacaact cctcataaag
     8101 agacagcaac caggatttat acaaggagga gaaaatgaaa gccatacggg aagcaatagc
     8161 atgatacaaa ggcattaaag cagcgtatcc acatagcgta aaaggagcaa catagttaag
     8221 aataccagtc aatctttcac aaattttgta atccagaggt tgattgtcga ctgcagttcg
     8281 aacccgggca tatgtctaga accggtggat ccctgaggag tgcggccgct ttacttgtac
     8341 agctcgtcca tgccgagagt gatcccggcg gcggtcacga actccagcag gaccatgtga
     8401 tcgcgcttct cgttggggtc tttgctcagg gcggactggg tgctcaggta gtggttgtcg
     8461 ggcagcagca cggggccgtc gccgatg
//
