LOCUS       sequence-180624-        9843 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses YFP-NaVII-III from the Synapsin promoter, for 
            neuron-specific and activity-independent live label of the Axon 
            Initial Segment (AIS).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9843)
  AUTHORS   Dumitrescu AS, Evans MD, Grubb MS
  TITLE     Evaluating Tools for Live Imaging of Structural Plasticity at the 
            Axon Initial Segment.
  JOURNAL   Front Cell Neurosci. 2016 Nov 23;10:268. eCollection 2016.
  PUBMED    27932952
REFERENCE   2  (bases 1 to 9843)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front Cell 
            Neurosci."; date: "2016-11-23"; volume: "10"; pages: "268. 
            eCollection 2016"
COMMENT     Sequence Label: pl-Synapsin-YFP-NaVII-III
FEATURES             Location/Qualifiers
     source          1..9843
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2632..3079
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     regulatory      3093..3102
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3099..3101,3102..3104,3105..3815)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3144..3165)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3405..3424)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3752..3773
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    4535..5123
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4588..4608)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5006..5017)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5126..5142)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5127..5143)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5648..5828
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5854..5871)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5860..6084
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6130..6558
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6217..6236)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6427..6448
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6567..6587)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6572..6901
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6752..6887
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6814..6833
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6949..6996
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7015..7389
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7519..7640
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7556..7575)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7610..7629
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7689..7705)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7689..7705)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7702..7724)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7713..7729
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7737..7743,7744..7761,7762..7767))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7782..7803
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7920..7937)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8091..8679)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8171..8190)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8850..9641,9642..9710))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9473..9492
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9711..9815)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta agtgtctaga ctgcagaggg ccctgcgtat gagtgcaagt
     2641 gggttttagg accaggatga ggcggggtgg gggtgcctac ctgacgaccg accccgaccc
     2701 actggacaag cacccaaccc ccattcccca aattgcgcat cccctatcag agagggggag
     2761 gggaaacagg atgcggcgag gcgcgtgcgc actgccagct tcagcaccgc ggacagtgcc
     2821 ttcgcccccg cctggcggcg cgcgccaccg ccgcctcagc actgaaggcg cgctgacgtc
     2881 actcgccggt cccccgcaaa ctccccttcc cggccacctt ggtcgcgtcc gcgccgccgc
     2941 cggcccagcc ggaccgcacc acgcgaggcg cgagataggg gggcacgggc gcgaccatct
     3001 gcgctgcggc gccggcgact cagcgctgcc tcagtctgcg gtgggcagcg gaggagtcgt
     3061 gtcgtgcctg agagcgcagt cgagaaaccg gtgccaccat ggtgagcaag ggcgaggagc
     3121 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt
     3181 tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca
     3241 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ttcggctacg
     3301 gcctgcagtg cttcgcccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg
     3361 ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca
     3421 agacccgcgc cgaggtgaag ttcgagggtg acaccctggt gaaccgcatc gagctgaagg
     3481 gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca
     3541 gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga
     3601 tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc
     3661 ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagctac cagtccgccc
     3721 tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg
     3781 ccgggatcac tctcggcatg gacgagctgt acaagagttc tttcagctca gacaacctgg
     3841 ctgccacaga tgacgataac gaaatgaaca acctccagat agccgtggga aggatgcaga
     3901 agggaattga ttttgttaaa aggaagatac gtgaattcat tcagaaagcc tttgtcagaa
     3961 agcagaaagc tttagatgaa atcaaaccgc tggaagatct gaataacaag aaagacagtt
     4021 gtatctccaa ccacacgacc atagaaatag gcaaggacct caattacctc aaagatggaa
     4081 acgggacgac cagtggcata ggcagcagtg tggagaagta tgtggtagat gagagtgatt
     4141 acatgtcgtt cataaacaac cccagcctca ccgtgactgt gcccatcgcc cttggagagt
     4201 ctgactttga aaacttaaac acggaagaat tcagtagtga gtcagatatg gaagaaagca
     4261 aggagaaatt gaatgcaact agctcatctg agggcagtac ggttgatata ggcgctccgg
     4321 cagagggaga gcagccagag gccgaaccgg aagaatcgct tgaaccggaa gcgtgtttca
     4381 cagaagactg tgtgagaaag ttcaagtgtt gtcagataag catagaagag ggcaaaggca
     4441 agctctggtg gaacttgagg aaaacgtgct acaagataag ctagctgtac aagtaagaat
     4501 tcgaaagggc gaattcgata tcaagcttat cgataatcaa cctctggatt acaaaatttg
     4561 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
     4621 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
     4681 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
     4741 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
     4801 gctcctttcc ggaactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
     4861 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
     4921 gtcggggaaa tcatcgtcct ttccttggct gctcgcctat gttgccacct ggattctgcg
     4981 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg
     5041 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat
     5101 ctccctttgg gccgcctccc cgcatcgata ccgtcgacct cgagacctag aaaaacatgg
     5161 agcaatcaca agtagcaata cagcagctac caatgctgat tgtgcctggc tagaagcaca
     5221 agaggaggag gaggtgggtt ttccagtcac acctcaggta cctttaagac caatgactta
     5281 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat
     5341 tcactcccaa cgaagacaag atatccttga tctgtggatc taccacacac aaggctactt
     5401 ccctgattgg cagaactaca caccagggcc agggatcaga tatccactga cctttggatg
     5461 gtgctacaag ctagtaccag ttgagcaaga gaaggtagaa gaagccaatg aaggagagaa
     5521 cacccgcttg ttacaccctg tgagcctgca tgggatggat gacccggaga gagaagtatt
     5581 agagtggagg tttgacagcc gcctagcatt tcatcacatg gcccgagagc tgcatccgga
     5641 ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     5701 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     5761 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     5821 ctctagcagg gcccgtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc
     5881 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg
     5941 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc
     6001 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg
     6061 ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg
     6121 ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
     6181 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
     6241 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
     6301 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
     6361 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
     6421 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
     6481 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
     6541 aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc
     6601 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg
     6661 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     6721 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     6781 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     6841 tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
     6901 aaagctcccg ggagcttgta tatccatttt cggatctgat cagcacgtgt tgacaattaa
     6961 tcatcggcat agtatatcgg catagtataa tacgacaagg tgaggaacta aaccatggcc
     7021 aagttgacca gtgccgttcc ggtgctcacc gcgcgcgacg tcgccggagc ggtcgagttc
     7081 tggaccgacc ggctcgggtt ctcccgggac ttcgtggagg acgacttcgc cggtgtggtc
     7141 cgggacgacg tgaccctgtt catcagcgcg gtccaggacc aggtggtgcc ggacaacacc
     7201 ctggcctggg tgtgggtgcg cggcctggac gagctgtacg ccgagtggtc ggaggtcgtg
     7261 tccacgaact tccgggacgc ctccgggccg gccatgaccg agatcggcga gcagccgtgg
     7321 gggcgggagt tcgccctgcg cgacccggcc ggcaactgcg tgcacttcgt ggccgaggag
     7381 caggactgac acgtgctacg agatttcgat tccaccgccg ccttctatga aaggttgggc
     7441 ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg
     7501 gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     7561 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     7621 aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg
     7681 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     7741 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     7801 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     7861 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     7921 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     7981 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     8041 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     8101 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     8161 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     8221 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     8281 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     8341 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     8401 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     8461 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     8521 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     8581 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     8641 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     8701 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     8761 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     8821 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     8881 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     8941 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     9001 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     9061 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     9121 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     9181 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     9241 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     9301 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     9361 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     9421 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     9481 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     9541 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     9601 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     9661 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     9721 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     9781 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     9841 gac
//
