LOCUS       sequence-180657-       10163 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Doxycycline inducible expression of human Notch1 ICD.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10163)
  TITLE     MIG-REST
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10163)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLIX-hN1ICD
FEATURES             Location/Qualifiers
     source          1..10163
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..23
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    140..161
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(176..181,182..199,200..206)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    214..230
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     219..241
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     238..254
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     238..254
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     273..293
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        275..293
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        321..547
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             548..728
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    775..900
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1393..1626
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1811..1855
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2068..2185
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2239..2553
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    2247..2265
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2283..2301
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2318..2336
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2354..2372
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2390..2408
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2425..2443
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2461..2479
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2526..2550
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2574..2598
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5021..5045)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5050..5091
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             5105..5122
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        5132..5642
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     5561..5579
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             5652..6248
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5652..5671)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6148..6168
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             6249..6302
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(6303..6920,6921..6926,6927..6962,6963..6965,6966..7001,
                     7002..7004,7005..7040,7041..7049)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(7089..7109)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7507..7518)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7723..7956
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8028..8149
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8065..8084)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8119..8138
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8189..8324
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        8324..8395
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8396..8464,8465..9256)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8614..8633)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9427..10015
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9916..9935
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
       61 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
      121 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
      181 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag
      241 gaaacagcta tgaccatgat tacgccaagc gcgcaattaa ccctcactaa agggaacaaa
      301 agctggagct gcaagcttaa tgtagtctta tgcaatactc ttgtagtctt gcaacatggt
      361 aacgatgagt tagcaacatg ccttacaagg agagaaaaag caccgtgcat gccgattggt
      421 ggaagtaagg tggtacgatc gtgccttatt aggaaggcaa cagacgggtc tgacatggat
      481 tggacgaacc actgaattgc cgcattgcag agatattgta tttaagtgcc tagctcgata
      541 cataaacggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
      601 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
      661 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
      721 ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct
      781 cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg
      841 agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc
      901 agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga
      961 aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca
     1021 gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa
     1081 ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc
     1141 tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag
     1201 gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg
     1261 aggaggagat atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat
     1321 tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag
     1381 agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg
     1441 cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca
     1501 gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg
     1561 gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca
     1621 gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa
     1681 tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg
     1741 ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa
     1801 ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa
     1861 ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg
     1921 cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg
     1981 atattcacca ttatcgtttc agacccacct cccaaccccg aggggacaat tctcgacctc
     2041 gagacaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag
     2101 tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag aattacaaaa
     2161 acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag atccactttg
     2221 gccgcgaatc gatatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt
     2281 actccctatc agtgatagag aacgatgtcg agtttactcc ctatcagtga tagagaacgt
     2341 atgtcgagtt tactccctat cagtgataga gaacgtatgt cgagtttact ccctatcagt
     2401 gatagagaac gtatgtcgag tttatcccta tcagtgatag agaacgtatg tcgagtttac
     2461 tccctatcag tgatagagaa cgtatgtcga ggtaggcgtg tacggtggga ggcctatata
     2521 agcagagctc gtttagtgaa ccgtcagatc gcctggagaa ttggctagca tcaacaagtt
     2581 tgtacaaaaa agcaggctct ttaaaggaac caattcagtc gactggatcc accatgcctg
     2641 agggcttcaa agtgtctgag gccagcaaga agaagcggcg ggagcccctc ggcgaggact
     2701 ccgtgggcct caagcccctg aagaacgctt cagacggtgc cctcatggac gacaaccaga
     2761 atgagtgggg ggacgaggac ctggagacca agaagttccg gttcgaggag cccgtggttc
     2821 tgcctgacct ggacgaccag acagaccacc ggcagtggac tcagcagcac ctggatgccg
     2881 ctgacctgcg catgtctgcc atggccccca caccgcccca gggtgaggtt gacgccgact
     2941 gcatggacgt caatgtccgc gggcctgatg gcttcacccc gctcatgatc gcctcctgca
     3001 gcgggggcgg cctggagacg ggcaacagcg aggaagagga ggacgcgccg gccgtcatct
     3061 ccgacttcat ctaccagggc gccagcctgc acaaccagac agaccgcacg ggcgagaccg
     3121 ccttgcacct ggccgcccgc tactcacgct ctgattccgc caagcgcctg ctggaggcca
     3181 gcgcagatgc caacatccag gacaacatgg gccgcacccc gctgcatgcg gctgtgtctg
     3241 ccgacgcaca aggtgtcttc cagatcctga tccggaaccg agccacagac ctggatgccc
     3301 gcatgcatga tggcacgacg ccactgatcc tggctgcccg cctggccgtg gagggcatgc
     3361 tggaggacct catcaactca cacgccgacg tcaacgccgt agatgacctg ggcaagtccg
     3421 ccctgcactg ggccgccgcc gtgaacaatg tggatgccgc agttgtgctc ctgaagaacg
     3481 gggctaacaa agatatgcag aacaacaggg aggagacacc cctgtttctg gccgcccggg
     3541 agggcagcta cgagaccgcc aaggtgctgc tggaccactt tgccaaccgg gacatcacgg
     3601 atcatatgga ccgcctgccg cgcgacatcg cacaggagcg catgcatcac gacatcgtga
     3661 ggctgctgga cgagtacaac ctggtgcgca gcccgcagct gcacggagcc ccgctggggg
     3721 gcacgcccac cctgtcgccc ccgctctgct cgcccaacgg ctacctgggc agcctcaagc
     3781 ccggcgtgca gggcaagaag gtccgcaagc ccagcagcaa aggcctggcc tgtggaagca
     3841 aggaggccaa ggacctcaag gcacggagga agaagtccca ggatggcaag ggctgcctgc
     3901 tggacagctc cggcatgctc tcgcccgtgg actccctgga gtcaccccat ggctacctgt
     3961 cagacgtggc ctcgccgcca ctgctgccct ccccgttcca gcagtctccg tccgtgcccc
     4021 tcaaccacct gcctgggatg cccgacaccc acctgggcat cgggcacctg aacgtggcgg
     4081 ccaagcccga gatggcggcg ctgggtgggg gcggccggct ggcctttgag actggcccac
     4141 ctcgtctctc ccacctgcct gtggcctctg gcaccagcac cgtcctgggc tccagcagcg
     4201 gaggggccct gaatttcact gtgggcgggt ccaccagttt gaatggtcaa tgcgagtggc
     4261 tgtcccggct gcagagcggc atggtgccga accaatacaa ccctctgcgg gggagtgtgg
     4321 caccaggccc cctgagcaca caggccccct ccctgcagca tggcatggta ggcccgctgc
     4381 acagtagcct tgctgccagc gccctgtccc agatgatgag ctaccagggc ctgcccagca
     4441 cccggctggc cacccagcct cacctggtgc agacccagca ggtgcagcca caaaacttac
     4501 agatgcagca gcagaacctg cagccagcaa acatccagca gcagcaaagc ctgcagccgc
     4561 caccaccacc accacagccg caccttggcg tgagctcagc agccagcggc cacctgggcc
     4621 ggagcttcct gagtggagag ccgagccagg cagacgtgca gccactgggc cccagcagcc
     4681 tggcggtgca cactattctg ccccaggaga gccccgccct gcccacgtcg ctgccatcct
     4741 cgctggtccc acccgtgacc gcagcccagt tcctgacgcc cccctcgcag cacagctact
     4801 cctcgcctgt ggacaacacc cccagccacc agctacaggt gcctgagcac cccttcctca
     4861 ccccgtcccc tgagtcccct gaccagtggt ccagctcgtc cccgcattcc aacgtctccg
     4921 actggtccga gggcgtctcc agccctccca ccagcatgca gtcccagatc gcccgcattc
     4981 cggaggcctt caagtaagcg gccgcactcg agatatctag acccagcttt cttgtacaaa
     5041 gtggttgcag gaaagccaat cccaaaccca ctcctaggtc tcgacagtac agcatagacc
     5101 ggtccaccac caccaccacc actaaggatc cggggttggg gttgcgcctt ttccaaggca
     5161 gccctgggtt tgcgcaggga cgcggctgct ctgggcgtgg ttccgggaaa cgcagcggcg
     5221 ccgaccctgg gtctcgcaca ttcttcacgt ccgttcgcag cgtcacccgg atcttcgccg
     5281 ctacccttgt gggccccccg gcgacgcttc ctgctccgcc cctaagtcgg gaaggttcct
     5341 tgcggttcgc ggcgtgccgg acgtgacaaa cggaagccgc acgtctcact agtaccctcg
     5401 cagacggaca gcgccaggga gcaatggcag cgcgccgacc gcgatgggct gtggccaata
     5461 gcggctgctc agcagggcgc gccgagagca gcggccggga aggggcggtg cgggaggcgg
     5521 ggtgtggggc ggtagtgtgg gccctgttcc tgcccgcgcg gtgttccgca ttctgcaagc
     5581 ctccggagcg cacgtcggca gtcggctccc tcgttgaccg aatcaccgac ctctctcccc
     5641 agcaattcac catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc
     5701 ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg
     5761 tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg
     5821 tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga
     5881 ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg
     5941 agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc
     6001 ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca
     6061 agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc
     6121 ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca
     6181 ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc
     6241 ccggtgccga aggtagaggt tctctcctca cttgtggtga tgttgaagaa aaccctggtc
     6301 caatgtctag actggacaag agcaaagtca taaacggagc tctggaatta ctcaatggtg
     6361 tcggtatcga aggcctgacg acaaggaaac tcgctcaaaa gctgggagtt gagcagccta
     6421 ccctgtactg gcacgtgaag aacaagcggg ccctgctcga tgccctgcca atcgagatgc
     6481 tggacaggca tcatacccac ttctgccccc tggaaggcga gtcatggcaa gactttctgc
     6541 ggaacaacgc caagtcatac cgctgtgctc tcctctcaca tcgcgacggg gctaaagtgc
     6601 atctcggcac ccgcccaaca gagaaacagt acgaaaccct ggaaaatcag ctcgcgttcc
     6661 tgtgtcagca aggcttctcc ctggagaacg cactgtacgc tctgtccgcc gtgggccact
     6721 ttacactggg ctgcgtattg gaggaacagg agcatcaagt agcaaaagag gaaagagaga
     6781 cacctaccac cgattctatg cccccacttc tgagacaagc aattgagctg ttcgaccggc
     6841 agggagccga acctgccttc cttttcggcc tggaactaat catatgtggc ctggagaaac
     6901 agctaaagtg cgaaagcggc gggccgaccg acgcccttga cgattttgac ttagacatgc
     6961 tcccagccga tgcccttgac gactttgacc ttgatatgct gcctgctgac gctcttgacg
     7021 attttgacct tgacatgctc cccgggtaac taagtaagga tcgatccaag atatcgtatt
     7081 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     7141 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
     7201 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
     7261 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
     7321 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     7381 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc
     7441 tttccatggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
     7501 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
     7561 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
     7621 ccgcctgttt cgcctcggcg tccggactag aggtaccttt aagaccaatg acttacaagg
     7681 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact
     7741 cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct
     7801 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     7861 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     7921 tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta
     7981 ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg
     8041 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     8101 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc
     8161 tctagctatc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     8221 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
     8281 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctttcaa atatgtatcc
     8341 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     8401 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     8461 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     8521 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     8581 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
     8641 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     8701 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     8761 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     8821 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     8881 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     8941 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     9001 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     9061 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     9121 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     9181 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     9241 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     9301 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     9361 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     9421 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     9481 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     9541 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     9601 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     9661 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     9721 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     9781 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     9841 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     9901 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     9961 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
    10021 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
    10081 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
    10141 cgctcgccgc agccgaacga ccg
//
