LOCUS       sequence-180683-        8945 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FLAG tagged mouse Par6 wild type - Binds to aPKC, Par3, Lgl, Cdc42.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 8945)
  AUTHORS   Nolan ME, Aranda V, Lee S, Lakshmi B, Basu S, Allred DC, Muthuswamy 
            SK.
  TITLE     The polarity protein Par6 induces cell proliferation and is 
            overexpressed in breast cancer.
  JOURNAL   Cancer Res. 2008 Oct 15;68(20):8201-9.
  PUBMED    18922891
REFERENCE   2  (bases 1 to 8945)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer 
            Res."; date: "2008-10-15"; volume: "68(20)"; pages: "8201-9"
COMMENT     Sequence Label: FLAG-mPar6a WT
FEATURES             Location/Qualifiers
     source          1..8945
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     47..66
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             135..734
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(135..154)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     631..651
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     gap             928..984
                     /estimated_length=57
     gap             1123..1143
                     /estimated_length=21
     promoter        1197..1296
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     1247..1267
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      1367..1376
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1373..1375,1376..1378,1379..1891)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLXYGXQCFXRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFXGDTLVNRIELKGIDFKEDGNILGHKLEYNYNXHNVYIMADKQKNGIK
                     VNFKIRHNIE"
     primer_bind     complement(1418..1439)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1679..1698)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     LTR             2192..2706
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     protein_bind    2868..2884
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2892..2898,2899..2916,2917..2922))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2937..2958
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3075..3092)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3246..3834)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3326..3345)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4005..4796,4797..4865))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4628..4647
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4866..4970)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5038..5056
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(5094..5116)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5216..5235
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5429..5451
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5579..5598
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     LTR             5798..6314
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
     misc_feature    6378..6719
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             6786..7202
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     7132..7154
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     7170..7186
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             7227..7292
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     primer_bind     complement(8608..8631)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 gggctcaggg gcggggcggg cgcccgaagg tcctccggag gcccggcatt ctgcacgctt
       61 caaaagcgca cgtctgccgc gctgttctcc tcttcctcat ctccgggcct ttcgacctgc
      121 agcccaagct taccatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg
      181 tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca
      241 ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc
      301 gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct
      361 ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg
      421 ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc
      481 accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg
      541 gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg
      601 tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct
      661 tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca
      721 agcccggtgc ctgacgcccg ccccacgacc cgcagcgccc gaccgaaagg agcgcacgac
      781 cccatgcatc gataaaataa aagattttat ttagtctcca gaaaaagggg ggaatgaaag
      841 accccacctg taggtttggc aagctagctt aagtaacgcc attttgcaag gcatggaaaa
      901 tacataactg agaatagaga agttccannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      961 nnnnnnnnnn nnnnnnnnnn nnnngttgaa tgtcgtgaag gaagcagttc ctctggaagc
     1021 ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg cagcggaacc ccccacctgg
     1081 cgacaggtgc ctctgcggcc aaaagccacg trtatmarac kgnnnnnnnn nnnnnnnnnn
     1141 nnncggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa
     1201 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt
     1261 aggcgtgtac ggtgggaggt ctatataagc agagctggtt tagtgaaccg tcagatccgc
     1321 tagaattctg cagtcgacgg taccgcgggc ccgggatcca ccggtcgcca ccatggtgag
     1381 caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt
     1441 aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct
     1501 gaccctgaag ttgatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac
     1561 caccctsrsc tacggcstgc agtgcttcrc ccgctacccc gaccacatga agcagcacga
     1621 cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga
     1681 cgacggcaac tacaagaccc gcgccgaggt gaagttcgak ggcgacaccc tggtgaaccg
     1741 catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga
     1801 gtacaactac aacrsccaca aygtctatat catggccgac aagcagaaga acggcatcaa
     1861 ggtgaacttc aagatccgcc acaacatcga ggacggcrgc gtgcagctcg ccgaccacta
     1921 ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag
     1981 cwmccagtcc aamctgagca aagaccccaa cgagaagcgc gatcacatgg tscwcstcga
     2041 ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gacgcccgcc
     2101 ccacgacccg cagcgcccga ccgaaaggag cgcacgaccc catgcatcga taaaataaaa
     2161 gattttattt agtctccaga aaaagggggg aatgaaagac cccacctgta ggtttggcaa
     2221 gctagcttaa gtaacgccat tttgcaaggc atggaaaata cataactgag aatagagaag
     2281 ttcagatcaa ggttaggaac agagagacag cagaatatgg gccaaacagg atatctgtgg
     2341 taagcagttc ctgccccggc tcagggccaa gaacagatgg tccccagatg cggtcccgcc
     2401 ctcagcagtt tctagagaac catcagatgt ttccagggtg ccccaaggac ctgaaatgac
     2461 cctgtgcctt atttgaacta accaatcagt tcgcttctcg cttctgttcg cgcgcttctg
     2521 ctccccgagc tcaataaaag agcccacaac ccctcactcg gcgcgccagt cctccgatag
     2581 actgcgtcgc ccgggtaccc gtgtatccaa taaaccctct tgcagttgca tccgacttgt
     2641 ggtctcgctg ttccttggga gggtctcctc tgagtgattg actacccgtc agcgggggtc
     2701 tttcatgggt aacagtttct tgaagttgga gaacaacatt ctgagggtag gagtcgaata
     2761 ttaagtaatc ctgactcaat tagccactgt tttgaatcca catactccaa tactcctgaa
     2821 atagttcatt atggacagcg cagaaagagc tggggagaat tgtgaaattg ttatccgctc
     2881 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     2941 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     3001 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     3061 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     3121 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     3181 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     3241 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     3301 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     3361 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     3421 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     3481 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     3541 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     3601 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     3661 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     3721 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     3781 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     3841 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     3901 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     3961 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     4021 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     4081 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     4141 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     4201 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     4261 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     4321 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     4381 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     4441 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     4501 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     4561 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     4621 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     4681 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     4741 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     4801 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     4861 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     4921 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     4981 cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat
     5041 aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga aaacctctga
     5101 cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa
     5161 gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca
     5221 tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta
     5281 aggagaaaat accgcatcag gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg
     5341 cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg
     5401 cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggcgcaa
     5461 ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca
     5521 tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg
     5581 tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga
     5641 tgcgtccggc gtagaggcga ttagtccaat ttgttaaaga caggatatca gtggtccagg
     5701 ctctagtttt gactcaacaa tatcaccagc tgaagcctat agagtacgag ccatagataa
     5761 aataaaagat tttatttagt ctccagaaaa aggggggaat gaaagacccc acctgtaggt
     5821 ttggcaagct agcttaagta acgccatttt gcaaggcatg gaaaatacat aactgagaat
     5881 agagaagttc agatcaaggt taggaacaga gagacagcag aatatgggcc aaacaggata
     5941 tctgtggtaa gcagttcctg ccccggctca gggccaagaa cagatggtcc ccagatgcgg
     6001 tcccgccctc agcagtttct agagaaccat cagatgtttc cagggtgccc caaggacctg
     6061 aaatgaccct gtgccttatt tgaactaacc aatcagttcg cttctcgctt ctgttcgcgc
     6121 gcttctgctc cccgagctca ataaaagagc ccacaacccc tcactcggcg cgccagtcct
     6181 ccgatagact gcgtcgcccg ggtacccgta ttcccaataa agcctcttgc tgtttgcatc
     6241 cgaatcgtgg actcgctgat ccttgggagg gtctcctcag attgattgac tgcccacctc
     6301 gggggtcttt catttggagg ttccaccgag atttggagac ccctgcccag ggaccaccga
     6361 cccccccgcc gggaggtaag ctggccagcg gtcgtttcgt gtctgtctct gtctttgtgc
     6421 gtgtttgtgc cggcatctaa tgtttgcgcc tgcgtctgta ctagttagct aactagctct
     6481 gtatctggcg gacccgtggt ggaactgacg agttctgaac acccggccgc aaccctggga
     6541 gacgtcccag ggactttggg ggccgttttt gtggcccgac ctgaggaagg gagtcgatgt
     6601 ggaatccgac cccgtcagga tatgtggttc tggtaggaga cgagaaccta aaacagttcc
     6661 cgcctccgtc tgaatttttg ctttcggttt ggaaccgaag ccgcgcgtct tgtctgctgc
     6721 agcgctgcag catcgttctg tgttgtctct gtctgactgt gtttctgtat ttgtctgaaa
     6781 attagggcca gactgttacc actcccttaa gtttgacctt aggtcactgg aaagatgtcg
     6841 agcggatcgc tcacaaccag tcggtagatg tcaagaagag acgttgggtt accttctgct
     6901 ctgcagaatg gccaaccttt aacgtcggat ggccgcgaga cggcaccttt aaccgagacc
     6961 tcatcaccca ggttaagatc aaggtctttt cacctggccc gcatggacac ccagaccagg
     7021 tcccctacat cgtgacctgg gaagccttgg cttttgaccc ccctccctgg gtcaagccct
     7081 ttgtacaccc taagcctccg cctcctcttc ctccatccgc cccgtctctc ccccttgaac
     7141 ctcctcgttc gaccccgcct cgatcctccc tttatccagc cctcactcct tctctaggcg
     7201 ccggaattag atctctcgag accatggact acaaagacca tgacggtgat tataaagatc
     7261 atgacatcga ttacaaggat gacgatgaca agcttgcggc cgcgaattcc atggccaggc
     7321 cgcagaggac tccggcgcgc agtcccgata gcatcgtcga ggtgaagagc aaatttgacg
     7381 ccgagttccg acgctttgca ctaccccgca cttcggtgag aggctttcag gagttctcgc
     7441 gattgctgtg tgtggtacac cagatccctg gcctggacgt cctgcttggc tatacggatg
     7501 ctcacggtga cttgctgccc ctcaccaacg atgacagttt gcaccgggcc ctggccagcg
     7561 ggcccccacc tctgcgcctc ttggttcaga aacgggcaga aggtgactcg agtggcctgg
     7621 cttttgcctc caactctcta caaaggcgca agaaagggct cctgctacga ccagtggcac
     7681 ctctgcgcac caggccaccc ttgttaatca gcttgcccca agatttccgc caggtgtctt
     7741 cagttataga tgtggaccta ctacctgaga cccaccgacg agtgaggctg cacaaacatg
     7801 gttcagaccg tcccctgggc ttctacattc gagatggcat gagtgttcgc gtggctcccc
     7861 agggcctgga gcgggttcca ggtatcttca tctcccgcct ggtacgtggg ggcctggctg
     7921 agagtacagg gctgctggcg gtcagtgatg agatccttga ggtcaacggc attgaggtgg
     7981 ccgggaagac cttggaccaa gtgacggaca tgatggtcgc caacagccac aacctcatcg
     8041 tcactgtcaa gcctgccaac cagcgtaata atgtggtacg gggggcatct gggcgtctga
     8101 cagggccttc ctctgtaggg cctgggccta ctgatcctga cagtgacgat gacagcagtg
     8161 acctggtcat tgagaatcgc caccctccct gttctaatgg gctgtctcag gggcccctgt
     8221 gctgggacct gcaacctggc tgcctacttc ctggtgctgg cagctctctg ccctccttgg
     8281 atagcagaga gcaagccaat tctggctggg ggaatggcat gcgaggtgat gttagcggat
     8341 tcagcctctg acaggcaaag atgcagccct tgtcacttta ggctgcggga acatggcctg
     8401 gactttccag ctgggtagag aagattagct tgctcacaga gccctctcag tctggtgaac
     8461 attaaaggtt ttctacaaat gtaaaaaaaa aaaaaaaaaa aaaaaanttt ccgcggccat
     8521 cggtaccagt cgactctaga ggatcccggg aggttaacga attaattcta ccgggtaggg
     8581 gaggcgcttt tcccaaggca gtctggagca tgcgctttag cagccccgct gggcacttgg
     8641 cgctacacaa gtggcctctg gcctcgcaca cattccacat ccaccggtag gcgccaaccg
     8701 gctccgttct ttggtggccc cttcgcgcca ccttctactc ctcccctagt caggaagttc
     8761 ccccccgccc cgcagctcgc gtcgtgcagg acgtgacaaa tggaagtagc acgtctcact
     8821 agtctcgtgc agatggacag caccgctgag caatggaagc gggtaggcct ttggggcagc
     8881 ggccaatagc agctttgctc cttcgctttc tgggctcaga ggctgggaag gggtgggtcc
     8941 ggggg
//
