LOCUS       sequence-180698-        8477 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor template for generation of SOX9-ntdTomato reporter cell lines.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8477)
  AUTHORS   Otonkoski
  TITLE     Otonkoski lab Reporter cell lines plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8477)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: SOX9-T2A-NLS-tdTomato-PGK-Puro
FEATURES             Location/Qualifiers
     source          1..8477
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(143..148,149..166,167..173)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     186..208
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     205..221
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             865..918
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             928..948
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             958..978
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             985..2415
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGRYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     protein_bind    2446..2479
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        2540..3039
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2575..2598)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     2972..2991
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             3079..3678
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APHNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3079..3098)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     protein_bind    4235..4268
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(4910..4929)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4911..4929)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4936..4953)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4936..4952)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4945..4967)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5093..5521
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5180..5199)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5390..5411
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             5865..6659
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5919..5938)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6529..6548
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     CDS             join(6677..6745,6746..7537)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6895..6914)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7708..8296
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8197..8216
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8450..8467
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcttg
      241 gtaccgagct cggatcccta cacgggcagc tacggcatca gcagcaccgc ggccaccccg
      301 gcgagcgcgg gccacgtgtg gatgtccaag cagcaggcgc cgccgccacc cccgcagcag
      361 cccccacagg ccccgccggc cccgcaggcg cccccgcagc cgcaggcggc gcccccacag
      421 cagccggcgg cacccccgca gcagccacag gcgcacacgc tgaccacgct gagcagcgag
      481 ccgggccagt cccagcgaac gcacatcaag acggagcagc tgagccccag ccactacagc
      541 gagcagcagc agcactcgcc ccaacagatc gcctacagcc ccttcaacct cccacactac
      601 agcccctcct acccgcccat cacccgctca cagtacgact acaccgacca ccagaactcc
      661 agctcctact acagccacgc ggcaggccag ggcaccggcc tctactccac cttcacctac
      721 atgaaccccg ctcagcgccc catgtacacc cccatcgccg acacctctgg ggtcccttcc
      781 atcccgcaga cccacagccc ccagcactgg gaacaacccg tctacacaca gctcactcga
      841 cctgctagcg tcgacggctc cggagagggc agaggaagtc tgctaacatg cggtgacgtc
      901 gaggagaatc ctggcccaat cgatggtcca aaaaagaaga gaaaggtatt cgatggtcca
      961 aaaaagaaga gaaaggtatt cgatatggtg agcaagggcg aggaggtcat caaagagttc
     1021 atgcgcttca aggtgcgcat ggagggctcc atgaacggcc acgagttcga gatcgagggc
     1081 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggc
     1141 ggccccctgc ccttcgcctg ggacatcctg tccccccagt tcatgtacgg ctccaaggcg
     1201 tacgtgaagc accccgccga catccccgat tacaagaagc tgtccttccc cgagggcttc
     1261 aagtgggagc gcgtgatgaa cttcgaggac ggcggtctgg tgaccgtgac ccaggactcc
     1321 tccctgcagg acggcacgct gatctacaag gtgaagatgc gcggcaccaa cttccccccc
     1381 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctccaccga gcgcctgtac
     1441 ccccgcgacg gcgtgctgaa gggcgagatc caccaggccc tgaagctgaa ggacggcggc
     1501 cactacctgg tggagttcaa gaccatctac atggccaaga agcccgtgca actgcccggc
     1561 tactactacg tggacaccaa gctggacatc acctcccaca acgaggacta caccatcgtg
     1621 gaacagtacg agcgctccga gggccgccac cacctgttcc tggggcatgg caccggcagc
     1681 accggcagcg gcagctccgg caccgcctcc tccgaggaca acaacatggc cgtcatcaaa
     1741 gagttcatgc gcttcaaggt gcgcatggag ggctccatga acggccacga gttcgagatc
     1801 gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc
     1861 aagggcggcc ccctgccctt cgcctgggac atcctgtccc cccagttcat gtacggctcc
     1921 aaggcgtacg tgaagcaccc cgccgacatc cccgattaca agaagctgtc cttccccgag
     1981 ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gtctggtgac cgtgacccag
     2041 gactcctccc tgcaggacgg cacgctgatc tacaaggtga agatgcgcgg caccaacttc
     2101 ccccccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc caccgagcgc
     2161 ctgtaccccc gcgacggcgt gctgaagggc gagatccacc aggccctgaa gctgaaggac
     2221 ggcggccgct acctggtgga gttcaagacc atctacatgg ccaagaagcc cgtgcaactg
     2281 cccggctact actacgtgga caccaagctg gacatcacct cccacaacga ggactacacc
     2341 atcgtggaac agtacgagcg ctccgagggc cgccaccacc tgttcctgta cggcatggac
     2401 gagctgtaca agtaagaatt ccgatcatat tcaataaccc ttaatataac ttcgtataat
     2461 gtatgctata cgaagttatt aggtctgaag aggagtttac gtccagccaa gcttaggatc
     2521 tcgacctcga aattctaccg ggtaggggag gcgcttttcc caaggcagtc tggagcatgc
     2581 gctttagcag ccccgctggg cacttggcgc tacacaagtg gcctctggcc tcgcacacat
     2641 tccacatcca ccggtaggcg ccaaccggct ccgttctttg gtggcccctt cgcgccacct
     2701 tctactcctc ccctagtcag gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg
     2761 tgacaaatgg aagtagcacg tctcactagt ctcgtgcaga tggacagcac cgctgagcaa
     2821 tggaagcggg taggcctttg gggcagcggc caatagcagc tttgctcctt cgctttctgg
     2881 gctcagaggc tgggaagggg tgggtccggg ggcgggctca ggggcgggct caggggcggg
     2941 gcgggcgccc gaaggtcctc cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct
     3001 gccgcgctgt tctcctcttc ctcatctccg ggcctttcga cctgcatcca tctagatctc
     3061 gagcagctga agcttaccat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac
     3121 gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc
     3181 cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc
     3241 acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg
     3301 gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc
     3361 atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg
     3421 ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac
     3481 cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc
     3541 ggggtgcccg ccttcctgga gacctccgcg ccccacaacc tccccttcta cgagcggctc
     3601 ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc
     3661 cgcaagcccg gtgcctgacg cccgccccac gacccgcagc gcccgaccga aaggagcgca
     3721 cgaccccatg catcgatgat atcagatccc cgggatgcag aaattgatga tctattaaac
     3781 aataaagatg tccactaaaa tggaagtttt tcctgtcata ctttgttaag aagggtgaga
     3841 acagagtacc tacattttga atggaaggat tggagctacg ggggtggggg tggggtggga
     3901 ttagataaat gcctgctctt tactgaaggc tctttactat tgctttatga taatgtttca
     3961 tagttggata tcataattta aacaagcaaa accaaattaa gggccagctc attcctccca
     4021 ctcatgatct atagatctat agatctctcg tgggatcatt gtttttctct tgattcccac
     4081 tttgtggttc taagtactgt ggtttccaaa tgtgtcagtt tcatagcctg aagaacgaga
     4141 tcagcagcct ctgttccaca tacacttcat tctcagtatt gttttgccaa gttctaattc
     4201 catcagaagc tggtcgagat ccggaaccct taatataact tcgtataatg tatgctatac
     4261 gaagttatta ggtccctcga agaggttcac taggcgcgcc cgaagggcga agatgtccga
     4321 gatgatccta aaaataaccg aagaaagaga ggaccaacca gaattccctt tggacatttg
     4381 tgtttttttg tttttttatt ttgttttgtt ttttcttctt cttcttcttc cttaaagaca
     4441 tttaagctaa aggcaactcg tacccaaatt tccaagacac aaacatgacc tatccaagcg
     4501 cattacccac ttgtggccaa tcagtggcca ggccaacctt ggctaaatgg agcagcgaaa
     4561 tcaacgagaa actggacttt ttaaaccctc ttcagagcaa gcgtggagga tgatggagaa
     4621 tcgtgtgatc agtgtgctaa atctctctgc ctgtttggac tttgtaatta tttttttagc
     4681 agtaattaaa gaaaaaagtc ctctgtgagg aatattctct attttaaata tttttagtat
     4741 gtactgtgta tgattcatta ccattttgag gggatttata catattttta gataaaatta
     4801 aatgctctta tttttccaac agctaaacta ctcttagttg aacagtgtgc cctagctttt
     4861 cttgcaacca gagcggccgc tcgagcatgc atctagaggg cccaattcgc cctatagtga
     4921 gtcgtattac aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
     4981 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
     5041 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggacgcgccc
     5101 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     5161 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     5221 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     5281 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     5341 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     5401 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     5461 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     5521 tttaacaaaa ttcagggcgc aagggctgct aaaggaagcg gaacacgtag aaagccagtc
     5581 cgcagaaacg gtgctgaccc cggatgaatg tcagctactg ggctatctgg acaagggaaa
     5641 acgcaagcgc aaagagaaag caggtagctt gcagtgggct tacatggcga tagctagact
     5701 gggcggtttt atggacagca agcgaaccgg aattgccagc tggggcgccc tctggtaagg
     5761 ttgggaagcc ctgcaaagta aactggatgg ctttcttgcc gccaaggatc tgatggcgca
     5821 ggggatcaag atctgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg
     5881 gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac
     5941 aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg
     6001 ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaggac gaggcagcgc
     6061 ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg
     6121 aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatccc
     6181 accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc
     6241 ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta
     6301 ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg
     6361 cgccagccga actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg
     6421 tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat
     6481 tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc
     6541 gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta
     6601 tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgaa
     6661 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     6721 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     6781 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     6841 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     6901 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     6961 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     7021 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     7081 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     7141 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     7201 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     7261 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     7321 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     7381 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     7441 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     7501 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     7561 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     7621 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     7681 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     7741 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     7801 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     7861 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     7921 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     7981 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     8041 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     8101 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     8161 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     8221 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     8281 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     8341 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     8401 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     8461 gagcgaggaa gcggaag
//
