LOCUS       sequence-180699-        9772 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor template for generation of NGN3-ntdTomato reporter cell lines.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9772)
  AUTHORS   Otonkoski
  TITLE     Otonkoski lab Reporter cell lines plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9772)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: NGN3-T2A-NLS-tdTomato-ires-puro-BGHpA-PGK-puro
FEATURES             Location/Qualifiers
     source          1..9772
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(143..148,149..166,167..173)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             715..768
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             778..798
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             808..828
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             835..2265
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGRYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     misc_feature    join(2287..2861,2862..2864,2865..2873)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2454..2471)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2681..2700
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             2874..3473
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3554..3571)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3560..3784
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(3831..3847)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3855..3871
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    3924..3957
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        4018..4517
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4053..4076)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4450..4469
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             4557..5156
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APHNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     protein_bind    5713..5746
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(6205..6224)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6206..6224)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6231..6248)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6231..6247)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6240..6262)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6388..6816
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6475..6494)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6685..6706
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             7160..7954
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7214..7233)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7824..7843
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     CDS             join(7972..8040,8041..8832)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8190..8209)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9003..9591
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9492..9511
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9745..9762
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcttg
      241 gtaccgagct cggatccccg gcctaagagc gagttggcac tgagcaagca gcgacggagt
      301 cggcgaaaga aggccaacga ccgcgagcgc aatcgaatgc acaacctcaa ctcggcactg
      361 gacgccctgc gcggtgtcct gcccaccttc ccagacgacg cgaagctcac caagatcgag
      421 acgctgcgct tcgcccacaa ctacatctgg gcgctgactc aaacgctgcg catagcggac
      481 cacagcttgt acgcgctgga gccgccggcg ccgcactgcg gggagctggg cagcccaggc
      541 ggttcccccg gggactgggg gtccctctac tccccagtct cccaggctgg cagcctgagt
      601 cccgccgcgt cgctggagga gcgacccggg ctgctggggg ccaccttttc cgcctgcttg
      661 agcccaggca gtctggcttt ctcagatttt ctcgctagcg tcgacggctc cggagagggc
      721 agaggaagtc tgctaacatg cggtgacgtc gaggagaatc ctggcccaat cgatggtcca
      781 aaaaagaaga gaaaggtatt cgatggtcca aaaaagaaga gaaaggtatt cgatatggtg
      841 agcaagggcg aggaggtcat caaagagttc atgcgcttca aggtgcgcat ggagggctcc
      901 atgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc
      961 cagaccgcca agctgaaggt gaccaagggc ggccccctgc ccttcgcctg ggacatcctg
     1021 tccccccagt tcatgtacgg ctccaaggcg tacgtgaagc accccgccga catccccgat
     1081 tacaagaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac
     1141 ggcggtctgg tgaccgtgac ccaggactcc tccctgcagg acggcacgct gatctacaag
     1201 gtgaagatgc gcggcaccaa cttccccccc gacggccccg taatgcagaa gaagaccatg
     1261 ggctgggagg cctccaccga gcgcctgtac ccccgcgacg gcgtgctgaa gggcgagatc
     1321 caccaggccc tgaagctgaa ggacggcggc cactacctgg tggagttcaa gaccatctac
     1381 atggccaaga agcccgtgca actgcccggc tactactacg tggacaccaa gctggacatc
     1441 acctcccaca acgaggacta caccatcgtg gaacagtacg agcgctccga gggccgccac
     1501 cacctgttcc tggggcatgg caccggcagc accggcagcg gcagctccgg caccgcctcc
     1561 tccgaggaca acaacatggc cgtcatcaaa gagttcatgc gcttcaaggt gcgcatggag
     1621 ggctccatga acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag
     1681 ggcacccaga ccgccaagct gaaggtgacc aagggcggcc ccctgccctt cgcctgggac
     1741 atcctgtccc cccagttcat gtacggctcc aaggcgtacg tgaagcaccc cgccgacatc
     1801 cccgattaca agaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc
     1861 gaggacggcg gtctggtgac cgtgacccag gactcctccc tgcaggacgg cacgctgatc
     1921 tacaaggtga agatgcgcgg caccaacttc ccccccgacg gccccgtaat gcagaagaag
     1981 accatgggct gggaggcctc caccgagcgc ctgtaccccc gcgacggcgt gctgaagggc
     2041 gagatccacc aggccctgaa gctgaaggac ggcggccgct acctggtgga gttcaagacc
     2101 atctacatgg ccaagaagcc cgtgcaactg cccggctact actacgtgga caccaagctg
     2161 gacatcacct cccacaacga ggactacacc atcgtggaac agtacgagcg ctccgagggc
     2221 cgccaccacc tgttcctgta cggcatggac gagctgtaca agtaatttaa agaattccgc
     2281 cccccccccc tctccctccc ccccccctaa cgttactggc cgaagccgct tggaataagg
     2341 ccggtgtgcg tttgtctata tgttattttc caccatattg ccgtcttttg gcaatgtgag
     2401 ggcccggaaa cctggccctg tcttcttgac gagcattcct aggggtcttt cccctctcgc
     2461 caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg
     2521 aagacaaaca acgtctgtag cgaccctttg caggcagcgg aaccccccac ctggcgacag
     2581 gtgcctctgc ggccaaaagc cacgtgtata agatacacct gcaaaggcgg cacaacccca
     2641 gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa tggctctcct caagcgtatt
     2701 caacaagggg ctgaaggatg cccagaaggt accccattgt atgggatctg atctggggcc
     2761 tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa aaacgtctag gccccccgaa
     2821 ccacggggac gtggttttcc tttgaaaaac acgatgataa tatggccaca accatgaccg
     2881 agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc
     2941 tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca
     3001 tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca
     3061 aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg
     3121 aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc
     3181 tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt
     3241 ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg
     3301 tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct
     3361 ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg
     3421 aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgacgcccgc
     3481 cccacgaccc gcagcgcccg accgaaagga gcgcacgacc ccatgcatcg aagatctaga
     3541 gctcgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc
     3601 cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag
     3661 gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag
     3721 gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct
     3781 atggcttctg aggcggaaag aacctgcagc ccaagcttgg cgtaatcatg gtcatagctg
     3841 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggcaattcc
     3901 gatcatattc aataaccctt aatataactt cgtataatgt atgctatacg aagttattag
     3961 gtctgaagag gagtttacgt ccagccaagc ttaggatctc gacctcgaaa ttctaccggg
     4021 taggggaggc gcttttccca aggcagtctg gagcatgcgc tttagcagcc ccgctgggca
     4081 cttggcgcta cacaagtggc ctctggcctc gcacacattc cacatccacc ggtaggcgcc
     4141 aaccgactcc gttctttggt ggccccttcg cgccaccttc tactcctccc ctagtcagga
     4201 agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc
     4261 tcactagtct cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta ggcctttggg
     4321 gcagcggcca atagcagctt tgctccttcg ctttctgggc tcagaggctg ggaaggggtg
     4381 ggtccggggg cgggctcagg ggcgggctca ggggcggggc gggcgcccga aggtcctccg
     4441 gaggcccggc attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct
     4501 catctccggg cctttcgacc tgcatccatc tagatctcga gcagctgaag cttaccatga
     4561 ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gccgtacgca
     4621 ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccggaccgcc
     4681 acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg
     4741 gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg
     4801 tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc
     4861 ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg
     4921 cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg
     4981 ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga
     5041 cctccgcgcc ccacaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg
     5101 tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctgacgcc
     5161 cgccccacga cccgcagcgc ccgaccgaaa ggagcgcacg accccatgca tcgatgatat
     5221 cagatccccg ggatgcagaa attgatgatc tattaaacaa taaagatgtc cactaaaatg
     5281 gaagtttttc ctgtcatact ttgttaagaa gggtgagaac agagtaccta cattttgaat
     5341 ggaaggattg gagctacggg ggtgggggtg gggtgggatt agataaatgc ctgctcttta
     5401 ctgaaggctc tttactattg ctttatgata atgtttcata gttggatatc ataatttaaa
     5461 caagcaaaac caaattaagg gccagctcat tcctcccact catgatctat agatctatag
     5521 atctctcgtg ggatcattgt ttttctcttg attcccactt tgtggttcta agtactgtgg
     5581 tttccaaatg tgtcagtttc atagcctgaa gaacgagatc agcagcctct gttccacata
     5641 cacttcattc tcagtattgt tttgccaagt tctaattcca tcagaagctg gtcgagatcc
     5701 ggaaccctta atataacttc gtataatgta tgctatacga agttattagg tccctcgaag
     5761 aggttcacta ggcgcgccga gctgtctgtc gctgtgctgt gggtgctaag ggtaagggag
     5821 agggagggag ccgggagccg tagagggtgg ccgacggcgg cggccctcaa aagcacttgt
     5881 tccttctgct tctccctggc tgacccctgg ccggcccagg cctccacggg ggcggcaggc
     5941 tgggttcatt ccccggccct ccgagccgcg ccaacgcacg caacccttgc tgctgcccgc
     6001 gcgaagtggg cattgcaaag tgcgctcatt ttaggcctcc tctctgccac caccccataa
     6061 tctcattcaa agaatactag aatggtagca ctacccggcc ggagccgccc accgtcttgg
     6121 gtcgccctac cctcactcaa gtctgtctgc ctctcagtct cttaccaccc ctcctccaat
     6181 gtgatttcta gagggcccaa ttcgccctat agtgagtcgt attacaattc actggccgtc
     6241 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
     6301 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
     6361 cagttgcgca gcctgaatgg cgaatggacg cgccctgtag cggcgcatta agcgcggcgg
     6421 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     6481 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     6541 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     6601 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     6661 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     6721 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
     6781 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaattcag ggcgcaaggg
     6841 ctgctaaagg aagcggaaca cgtagaaagc cagtccgcag aaacggtgct gaccccggat
     6901 gaatgtcagc tactgggcta tctggacaag ggaaaacgca agcgcaaaga gaaagcaggt
     6961 agcttgcagt gggcttacat ggcgatagct agactgggcg gttttatgga cagcaagcga
     7021 accggaattg ccagctgggg cgccctctgg taaggttggg aagccctgca aagtaaactg
     7081 gatggctttc ttgccgccaa ggatctgatg gcgcagggga tcaagatctg atcaagagac
     7141 aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc
     7201 ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc
     7261 cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc
     7321 cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg
     7381 cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt
     7441 gggcgaagtg ccggggcagg atctcctgtc atcccacctt gctcctgccg agaaagtatc
     7501 catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga
     7561 ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga
     7621 tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct
     7681 caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc
     7741 gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt
     7801 ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg
     7861 cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat
     7921 cgccttctat cgccttcttg acgagttctt ctgaattgaa aaaggaagag tatgagtatt
     7981 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     8041 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     8101 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     8161 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
     8221 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     8281 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     8341 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     8401 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     8461 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     8521 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     8581 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     8641 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     8701 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     8761 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     8821 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     8881 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     8941 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     9001 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     9061 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     9121 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     9181 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     9241 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     9301 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     9361 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     9421 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     9481 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     9541 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     9601 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     9661 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     9721 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga ag
//
