LOCUS       sequence-180701-       10612 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Drosophila laccase2 minigene plasmid that expresses both a linear 
            RNA and a circular RNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10612)
  AUTHORS   Liang D, Tatomer DC, Luo Z, Wu H, Yang L, Chen LL, Cherry S, Wilusz 
            JE
  TITLE     The Output of Protein-Coding Genes Shifts to Circular RNAs When the 
            Pre-mRNA Processing Machinery Is Limiting.
  JOURNAL   Mol Cell. 2017 Nov 9. pii: S1097-2765(17)30835-3. doi: 
            10.1016/j.molcel.2017.10.034.
  PUBMED    29174924
REFERENCE   2  (bases 1 to 10612)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2017 Nov 9. pii: S1097-2765(17)30835-3. doi: 
            10.1016/j.molcel.2017.10.034."
COMMENT     Sequence Label: Hy_pMT Laccase2 Exons 1-3
FEATURES             Location/Qualifiers
     source          1..10612
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(952..970)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1038..1142
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1143..1211,1212..2003)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1361..1380)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2174..2762
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2663..2682
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2916..2933
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    3643..3764
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        complement(7254..7680)
                     /label=MT promoter
                     /note="Drosophila metallothionein promoter"
     polyA_signal    7681..7815
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(8240..8260)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          8390..8455
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             complement(8818..9843)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(8818..9843)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(9889..10168)
                     /label=copia promoter
                     /note="strong promoter from the Drosophila transposable 
                     element copia (Sinclair et al., 1986)"
ORIGIN
        1 tgttaaagtc gctgatagct tcgggccgct tgttcgcaat gataacgaca gtgtaacaaa
       61 ttccagaggg gagccccttc caaaatacga gattccaaaa aaaaaaaaac aaaaaaaaga
      121 agaagcgttt cgaaactctg aagaaaaccc agagaagata agagcgtact tgacagcata
      181 tagcaaaact attgtacatt tccaaatttc aattggaatc gaaacgggaa cagtcaatcg
      241 cttgggttat cggaataatc gcctcaattg cgtgaaccta tcgacacaaa gtgcattggt
      301 attgagtcaa agtaaggttt gcttatatta actttcatag attgcatatt tttgccggct
      361 tattcatggg aataagaaat gtttgagctg aactcgatag ctcaacaata acacgacttg
      421 gtgtcaatta aatgataata cgtgactaga ccatggaaat tcaatgtatt gctattggat
      481 acaatacata tacaagtagt tgttgaaaag aaagcagcga aaactggtca ggtaatgagt
      541 catcatcatc ggagtcatct tgactgatta gcacaaaagc cagttctcat ctgtatgcaa
      601 atggcttaaa cgcttctacg tacataagta gttgatgttg ttaaacagga acttaagatg
      661 ccgccaacaa cagtttccac agcaattaaa cccaaatttt taattgcgca taacaggcgt
      721 ctttcatgtt cctgtccgca tttgacattg atctccttct gaatcctgat tcgataatat
      781 gtaatcttgg ggccatcaat cacgcagtca gcagcgattt gatatatgta tatataacac
      841 catattattc aaagccgttt gccttgctcg gttgatgagg aaattggtga aaaactcgaa
      901 cttttgataa aaaaaaaatt cgtacaaatg tgaattcttg aagacgaaag ggcctcgtga
      961 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca
     1021 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     1081 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     1141 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     1201 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     1261 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     1321 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     1381 cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     1441 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     1501 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     1561 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     1621 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     1681 tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     1741 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     1801 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     1861 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     1921 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     1981 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     2041 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     2101 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     2161 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     2221 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     2281 aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt
     2341 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     2401 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     2461 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     2521 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     2581 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     2641 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     2701 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     2761 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     2821 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     2881 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     2941 aagagcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat
     3001 gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc
     3061 ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac
     3121 aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg
     3181 cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc
     3241 acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg
     3301 tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg
     3361 ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg
     3421 ccgaggcggc ataaatcgcc gtgacgatca gcggtccaat gatcgaagtt aggctggtaa
     3481 gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca
     3541 tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg
     3601 ccatccagcc tcgcgtcggc ggcccctagt ggatccacta gttaagatac attgatgagt
     3661 ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg
     3721 ctattgcttt atttgtaacc attataagct gcaataaaca agttgcggcc gcattgtatt
     3781 tacatcaaca ggatgcacag gctcaccatc ggttgctata accgtcatgc cgtgaccctc
     3841 aattgttacc tgtgctggac agactgaagc aaatgcattt atcattcgaa agcggtaacg
     3901 acgtcctggc gtaattgtga atatttccaa cggagtatta gtcataaatc cagtgtttgg
     3961 gtcacgaaac tggccttttc cgttaatcaa catagactcc ggatcttggc ctgtgttaac
     4021 agccaagcgt ccaggatatc tctctgctgc atcttcatgc aaccaatcac tgataagcat
     4081 tatatgtgtt gttaaatcaa aatcatataa atgggaatta gggtcccttg atggtggctg
     4141 tcgaaccacg acgctgccgt agagtccatc tagtttctgt agaccggtat gagcatgcca
     4201 aaaatgtgtc ccagcgtttc ctgtccactg ataacggaag gtattaccct gctgaatggg
     4261 gcactgagtg acgaagggca ccccatcata atactgcgat ccacgttgcc atataccgtg
     4321 ccagtgtata gttacttcca ttccctccat atggttctcc acatcaataa cgactttgtc
     4381 gttttcacac acttgaatgc ttggcccggg tatcatacga ttcgcagtta gaattccacg
     4441 ttcaactcca tcagctaaga cacactgaca atggctccaa accgtattcg ttgcgtttgg
     4501 agtacatact tgacacgcac tgtgtgtaaa taattataat tataattagg tcaatccgat
     4561 aaaaatatat taatttgatt tatttatttt aaacttgctc aattgtaagg aaagacatat
     4621 taagtgtagc caatattggt gaataaaatg aaaaaatatt taaaaattgt ttaacagtgt
     4681 ttgtgtgtct atcttgggag tgtaggtgaa agggggcgtg accagggttt ctggcggccg
     4741 cctgaggtgc cacagactaa ttgtgtctcc aatgttggca ataattgaag acataatttt
     4801 aggtgctgct ggagggtgcg agacccgaca atgagtattt ttgtcttcaa tgtgcctgcg
     4861 ccatgtgagt cttatgtcga ggaggttaca ccttaagccc aaataaatat tcatattaaa
     4921 taaaaagcta taatcgatat tcatatgatt aaattcattt tcattgtata taaaaaataa
     4981 gtcgatttcc ccaactatca gatactcgtt actcagctag tgtgaatgcg aacgcgaaat
     5041 ttaataattt cataatcggg gatatcgata gatattgggg aataaaatga gaaaaaattt
     5101 gataattgct caatagtgtg ggcgtgacca aattggcggc tttagggcgt taacgcgtga
     5161 caaaaagttt tttggcaaat tgatggaaat ttacacgagt aatacaatta tgaaaaaatt
     5221 tcgaaaatag ttcaaaaatg tgggcatggc agttttgggc aatttgagaa agttaaagcg
     5281 tggcagcatg gatcaacaaa cttgctctgc gtccttgtct ctagaatctg tatgctaaaa
     5341 ctcatccttc tagcttttat agttcctgat gtctcggcgg taataaaaca ataagacaga
     5401 cggacatggc gagatcgact cggctattga tccttattaa gaatatatat actttacatg
     5461 gtcggaaatg ctttattctt cctgttacat acttttcaac gaatctacaa tagtatacaa
     5521 tagtatagaa actgcctatt aaaaaatcga atatttctag taaaaaattt tggaattttg
     5581 aatacttacg ctgagctccc cggggctccc aatactgtgt agtattccaa cgtaaaatga
     5641 tagtagcaaa ttctcggagg ctcgccttcg cgacaagcgc gtgcacattc atcaggagca
     5701 gatagcgcag gattgcgccg cagctcggca gttgcactag tagagaaatc tatgtggcgg
     5761 aatggagatt ttgggttggg ttccacgacg gttcctgtga tcttattagc tggataacgt
     5821 gcagaggtcg gtagactccc gcgtgagttt aggtaaccgc ttccagcggt tagggctggc
     5881 ttagtaccag ctaggattga ggatggagct ccagacccaa ggctattgcc atttaatgga
     5941 gcgggcccag agtggcttcc acctatagac gggtgagtct gcacaaggcc gtgtgttgcc
     6001 gaaaaaaatg gtgaatcgaa tggagttgaa ctactaggag tggccacatg ttgctgccag
     6061 aaggacccca ttggtgtaag atctggttga ttttggtacc tttctgcata aaataaaaaa
     6121 aatatatata acaataaaac ttagtctcga tttagcttat tattataccc ataaatcgta
     6181 cagtaaaaag gtatactaga ttcgttgaaa aataggtaac atgtagaagg aagcggtata
     6241 aagtatatat attcttgatc aggatcaaag cctagtcgat gtaaccctgt cctcgcctac
     6301 tctaacgccc taaaaccgcc caaaactgtc acgcccacgt gtttaaaatt ttgttgtatt
     6361 ttttcataat tttataagtc ttgtaaattt ctagcgattt gccaaaaaac tttttgccac
     6421 gccctaaacc cgcccaaaat ttatttattt aaatttattc cccaatatct atcgatatcc
     6481 gagaaaactg ataaaatttt gggttcgcat tccccctaga taagtaatgg gtatcagata
     6541 gtcgggaaac tatactatac gactatagca ctctctcttg ttttttcttt ttgtagctta
     6601 ctggcttcgc aacagaattt aatgtttttg aagtcgttat tacagagacc aaataaaagt
     6661 cgacaaagat caatgacttg agagcaccgg aactcagtca tttctcaatg ctcgagccca
     6721 aaagatcccc caaatctttg gaacacccct tggaaatata gtcatgttta gatgtatacg
     6781 agtatatacg cattgcactt aaataatcat tttaatgaaa ggaatatgta ctgtcgacaa
     6841 atcacaaaat gatatttcaa gacttcttag gtgaaaaaaa ttaccaagat gggatttcct
     6901 tacctttctt aggttgattt tgctgtgagt tggcaacacg tactgcaaat acttcaggaa
     6961 aattggaaaa tgcaccccat atcaggaacg aaataaaagt ttggtaaaat ccgttaagat
     7021 ccatggcttt catagtcgta tgaatggcgt ataattttat ttttcactat tcttcttgta
     7081 tttttattaa ttcctttaag ctatttaact atactttggg tggatgttca caacataaga
     7141 ctatatattt ttaaagtatg tgtactaagc aatagcaaca ccacctgaat tgattacggc
     7201 ttaatgaagt acgagcaacg cgtttaagca ccaaactgaa gctcgagatc ccccctttag
     7261 ttgcactgag atgattcact tgattttgct gctgaccaca actgatgcac attggccaga
     7321 tgctctcggt tttatacatt ttggaaacgc ggctttacac acgggtcttt cgttcgattc
     7381 acctcttttg cacacgccgg ccggctccaa attcgagtgg agacgtgtgc agaagctttt
     7441 gttctgcccg cgaagaattg gttcggttct ccactttggg gacttgtatg ggatgcaaag
     7501 ctcttggtgg ggcacatggg gcatgcgcag gcactcttac ttgcgtacca catatgtatg
     7561 ggggtggcgg tgggcggtgg ggggccggag ttctgggtct cttatcggaa ccagaggata
     7621 ggacggcctg cagcaaagag ctagtcacat cgggcaccac atcctgtcct gcaacgaatt
     7681 gatccagaca tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga
     7741 aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc
     7801 tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag
     7861 gtgtgggagg ttttttaaag caagtaaaac ctctacaaat gtggtatggc tgattatgat
     7921 ctctagtcaa ggcactatac atcaaatatt ccttattaac ccctttacaa attaaaaagc
     7981 taaaggtaca caatttttga gcatagttat taatagcaga cactctatgc ctgtgtggag
     8041 taagaaaaaa cagtatgtta tgattataac tgttatgcct acttataaag gttacagaat
     8101 atttttccat aattttcttg tatagcagtg cagctttttc ctttgtggtg taaatagcaa
     8161 agcaagcaag agttctatta ctaaacacag catgactcaa aaaacttagc aattctgaag
     8221 gaaagtcctt ggggtcttct acctttctct tcttttttgg aggagtagaa tgttgagagt
     8281 cagcagtagc ctcatcatca ctagatggca tttcttctga gcaaaacagg ttttcctcat
     8341 taaaggcatt ccaccactgc tcccattcat cagttccata ggttggaatc taaaatacac
     8401 aaacaattag aatcagtagt ttaacacatt atacacttaa aaattttata tttaccttag
     8461 agctttaaat ctctgtaggt agtttgtcca attatgtcac accacagaag taaggttcct
     8521 tcacaaagat ctgcacccaa tcggcaggca cgggcggcga tctccaatct gcgggatcag
     8581 tcagatcacc cgagtgcgtg ggcatgacaa tcgtgccctg gggaccaaca caatccagaa
     8641 gggcctgaat cactgcgacc ggccctcccg cgacccagcc gagcgagctt agcgaactgt
     8701 ggacgagaac tgtgccacca agcgtaaggc cgttctctcg catttgcctt gctaggctcg
     8761 cgcgagttgc tggctgaggc gttctcgaaa tcagctcttg ttcggtcggc atctactcta
     8821 ttcctttgcc ctcggacgag tgctggggcg tcggtttcca ctatcggcga gtacttctac
     8881 acagccatcg gtccagacgg ccgcgcttct gcgggcgatt tgtgtacgcc cgacagtccc
     8941 ggctccggat cggacgattg cgtcgcatcg accctgcgcc caagctgcat catcgaaatt
     9001 gccgtcaacc aagctctgat agagttggtc aagaccaatg cggagcatat acgcccggag
     9061 ccgcggcgat cctgcaagct ccggatgcct ccgctcgaag tagcgcgtct gctgctccat
     9121 acaagccaac cacggcctcc agaagaagat gttggcgacc tcgtattggg aatccccgaa
     9181 catcgcctcg ctccagtcaa tgaccgctgt tatgcggcca ttgtccgtca ggacattgtt
     9241 ggagccgaaa tccgcgtgca cgaggtgccg gacttcgggg cagtcctcgg cccaaagcat
     9301 cagctcatcg agagcctgcg cgacggacgc actgacggtg tcgtccatca cagtttgcca
     9361 gtgatacaca tggggatcag caatcgcgca tatgaaatca cgccatgtag tgtattgacc
     9421 gattccttgc ggtccgaatg ggccgaaccc gctcgtctgg ctaagatcgg ccgcagcgat
     9481 cgcatccatg gcctccgcga ccggctgcag aacagcgggc agttcggttt caggcaggtc
     9541 ttgcaacgtg acaccctgtg cacggcggga gatgcaatag gtcaggctct cgctgaattc
     9601 cccaatgtca agcacttccg gaatcgggag cgcggccgat gcaaagtgcc gataaacata
     9661 acgatctttg tagaaaccat cggcgcagct atttacccgc aggacatatc cacgccctcc
     9721 tacatcgaag ctgaaagcac gagattcttc gccctccgag agctgcatca ggtcggagac
     9781 gctgtcgaac ttttcgatca gaaacttctc gacagacgtc gcggtgagtt caggcttttt
     9841 catatctcat tgcccccgga cgagcgccaa gcttggctgc aggtccataa cctgttgtaa
     9901 tttataattt atatttcctt tcttaataaa taaataaata gtcaagttta tgtttgagtt
     9961 ttatgattta tattttaagt tatttcaact gcaacaccag caccacgacc tacttacagc
    10021 aaaaaacgta caagaaggaa agaaggaata aaaagagtgg tattctctta caatatgttt
    10081 tatggcataa aaggtgtggc cattcatatc aaatataaag tagtgttgtt taacgttact
    10141 tttgtaggtt gaatagtata ttccaacaga tgatgagggg ttcccaatcc taaacccatt
    10201 tgccgttccc agaagcatga aaccaccacg caccggaaac aacgttaaat gcgtaacaag
    10261 gcgcttttta atttaaccga tccaaataaa agaattaaat aaatggaatt gtgtccacat
    10321 gcaaagcagt tggaggacag cagcgttatc agcatttcga agcgcacgaa aacgcatgac
    10381 gaacgggcgg cttaattgaa attgtcattg attcaatacg caacatgcag aggagcatat
    10441 catgatggtc ggttcgttct tcgtttcact gggaaaaagc gaaattgaat ccattttgat
    10501 tgcgtttgtg tcgcaagagc aacacattgc ctgcgccaca tcgcgttcgc ttatcaacgg
    10561 gcgaagaaag gaaaatgtac atttaattgg tcgtaaaaaa gtgcattgta aa
//
