LOCUS       sequence-180704-        5550 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses N-terminally His6, Xpress and HA-tagged BIG1(E793K) in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5550)
  AUTHORS   Shin HW, Morinaga N, Noda M, Nakayama K
  TITLE     BIG2, a guanine nucleotide exchange factor for ADP-ribosylation 
            factors: its localization to recycling endosomes and implication in 
            the endosome integrity.
  JOURNAL   Mol Biol Cell. 2004 Dec;15(12):5283-94. Epub 2004 Sep 22.
  PUBMED    15385626
REFERENCE   2  (bases 1 to 5550)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell."; date: "2004-12"; volume: "15(12)"; pages: "5283-94. Epub 
            2004 Sep 22"
COMMENT     Sequence Label: pcDNA4c/His-Xpress-HA-hBIG1(E793K)
FEATURES             Location/Qualifiers
     source          1..5550
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgtatgagg ggaagaagac gaagaacatg ttcctgaccc gggctctgga gaagatattg
       61 gccgacaagg aagtgaagaa ggcgcatcac tcccagctgc gcaaagcttg cgaggtggcg
      121 ttagaggaaa taaaagcgga aactgaaaaa cagagtcctc ctcatggaga agcaaaagct
      181 ggatcaagca cccttccacc agtgaaatca aagacaaatt ttattgaagc agacaagtac
      241 ttcttgcctt ttgagttggc atgccagtcc aaatgtcctc gcatagttag tacatctcta
      301 gattgcttac agaaacttat tgcttatggg cacttgactg gcaatgctcc agatagtaca
      361 acaccaggca aaaaattaat tgatagaatt attgaaacaa tatgtggctg ctttcagggc
      421 cctcagacag atgaaggagt tcagctgcag ataataaagg ctttacttac tgcagtaaca
      481 tcacaacaca tagaaattca tgaagggact gtactgcaag ctgtgagaac atgttacaat
      541 atctacttag caagcaaaaa tctcatcaat cagacaacag ccaaagctac tctcactcag
      601 atgctaaatg ttatctttgc acgcatggaa aaccaagcat tacaggaagc aaaacaaatg
      661 gaaaaagaaa ggcatcggca gcatcatcat ctgttacagt ctccagtaag ccatcacgag
      721 cctgaatcac ctcaacttag atatttgcca cctcagactg ttgatcatat atcccaagaa
      781 cacgaagggg accttgacct ccatacaaat gatgtagata aaagtcttca ggatgacaca
      841 gagcctgaaa atggatctga tatttccagt gcagaaaatg aacagactga agctgatcag
      901 gcaactgcag ctgaaacatt atctaaaaat gaagtgttat atgacggaga aaaccatgat
      961 tgtgaggaaa agccacaaga cattgtacag aacattgtag aagaaatggt gaacattgtt
     1021 gttggagata tgggagaagg gactactata aatgcaagtg cagatggcaa cattggaact
     1081 atagaggatg gtagtgacag tgaaaatatt caagcaaatg gaattccagg aacaccaatt
     1141 tctgttgcat atacaccatc cttacctgat gatagattgt cagtctcttc caatgatact
     1201 caggaatctg gaaattcttc aggaccttca cctggtgcta agttttccca cattttacaa
     1261 aaggatgcct ttctagtatt caggtcattg tgtaaactgt caatgaaacc actgtcagat
     1321 ggaccaccag atccaaagtc tcatgaacta cgatccaaga ttctttcatt gcagttactt
     1381 ctatccattc tgcagaatgc aggacctatt ttcaggacaa atgagatgtt tattaatgct
     1441 attaagcagt atctttgtgt tgcactctca aaaaatggag tctcatctgt tccagaggtt
     1501 tttgagcttt ctctttctat atttcttact ttgttgtcaa atttcaagac acatctgaag
     1561 atgcaaattg aggtgttctt taaagaaatt ttcttataca ttttggaaac ttctaccagc
     1621 tcatttgatc acaaatggat ggttattcag acactgacga ggatttgtgc agatgctcag
     1681 agtgtagtgg atatttatgt aaactatgac tgtgacttaa atgcagccaa tatatttgaa
     1741 agactagtaa atgatctatc aaaaattgct caaggaaggg gcagtcaaga acttggtatg
     1801 agtaatgttc aggaattgag cctgaggaaa aaaggtttag aatgcttagt gtcgattttg
     1861 aagtgtatgg ttgaatggag taaggatcag tatgtgaatc ccaactccca gacaactctt
     1921 ggtcaggaaa aaccctcaga gcaagagatg agtgaaatca aacaccctga gacaataaac
     1981 agatacggaa gtttaaattc cctggagtca acatcatcat caggaatagg cagctacagt
     2041 acacagatgt ctggcactga taatccagaa caatttgagg tcctaaagca acaaaaagaa
     2101 ataatagaac aagggataga tttatttaat aagaaaccaa agagaggaat acagtacctc
     2161 caagaacaag ggatgcttgg caccacacct gaagatattg cccaattctt acatcaagag
     2221 gaaagattag actctactca agtgggtgag ttcctgggag ataatgataa atttaacaaa
     2281 gaagtcatgt atgcatatgt ggaccaacat gacttttcag gaaaagactt cgtttcagcc
     2341 cttcgtatgt ttctagaagg atttcgtctt ccaggggaag ctcagaaaat cgatcgatta
     2401 atggaaaaat ttgctgcaag atacctagaa tgcaaccaag gacaaactct ctttgctagt
     2461 gcggatacag cttatgtttt ggcttattca attatcatgt tgaccacaga ccttcacagt
     2521 ccacaggtga aaaataaaat gacaaaggaa caatacatta agatgaatag aggtatcaat
     2581 gacagtaaag accttcctga agagtatcta tcagccatct ataatgaaat agctgggaaa
     2641 aagatatcaa tgaaagaaac aaaagaacta acaatcccta caaaatcaag taaacagaat
     2701 gtagccagtg aaaaacaaag aagacttctg tataacttag aaatggagca gatggccaag
     2761 acagctaaag cactcatgga ggccgtgagc catgttcagg caccctttac aagtgcaaca
     2821 catttggagc atgtgaggcc catgtttaag ttggcttgga cgccttttct ggctgcattc
     2881 agtgtgggtc tacaagattg tgatgatact gaagtagcct ctctttgcct ggaaggtata
     2941 agatgtgcaa tcagaattgc atgcattttc agcattcagc tggagagaga tgcatatgtc
     3001 caggcactag caagatttac cttactcaca gtgagttccg gtattactga aatgaaacag
     3061 aagaacattg acacaataaa aacacttatc acagtggctc atacagatgg aaattattta
     3121 ggaaattcat ggcatgagat tctgaagtgc atcagtcagt tggagctggc acagcttata
     3181 ggaactggag tgaaacctcg atacatttct ggaacagtgc gaggcagaga aggatctctt
     3241 actggaacaa aagatcaggc tcctgatgaa tttgtgggtt tagggctagt tggaggaaat
     3301 gtggactgga aacagatagc aagtattcag gaatccattg gagaaaccag ttctcagagt
     3361 gttgttgttg ctgtagatag aatattcaca ggatctacaa ggctagatgg aaatgccatt
     3421 gtggattttg tccgttggct ctgtgctgtg tctatggatg aattactttc cacgacacac
     3481 ccaagaatgt ttagtctaca aaaaatagta gaaatatcat attacaacat gggaagaata
     3541 agattacagt ggtctcgaat ttgggaagtt attggagatc attttaataa ggttgggtgt
     3601 aatcctaatg aagatgtagc tatttttgca gtagactcct tgaggcagtt gtcaatgaag
     3661 ttcttagaga aaggggagct tgctaacttc agattccaga aggatttctt aagacctttt
     3721 gaacatataa tgaaacggaa caggtctcca acaattcgag atatggttgt acggtgtata
     3781 gcacagatgg ttaattctca agctgctaac attcgatctg gatggaagaa cattttctct
     3841 gtatttcatc tagctgcatc tgatcaagat gaaagcatag tggaacttgc attccaaaca
     3901 accgggcaca ttgtcaccct tgtatttgaa aaacactttc cagcgaccat tgattctttc
     3961 caggatgcag tgaagtgttt gtctgaattt gcgtgcaatg cagctttccc agacacaagt
     4021 atggaagcaa ttcgacttat tcgccattgt gcaaaatatg tgtctgatag acctcaggct
     4081 ttcaaggaat acacaagcga tgatatgaac gtagcacctg aagacagggt gtgggtgaga
     4141 ggatggttcc caattctctt tgagttatcc tgtatcatca atagatgcaa attagatgta
     4201 agaaccaggg gtttaacagt aatgtttgaa ataatgaaaa catatggcca cacttatgag
     4261 aaacactggt ggcaggattt atttagaatt gttttcagaa tctttgacaa tatgaaattg
     4321 ccagaacaac agacagagaa agctgaatgg atgacaacaa cttgcaatca tgcactttat
     4381 gcaatctgtg atgtattcac tcagtattta gaagtactca gtgatgtact tttggatgac
     4441 atttttgctc agctctactg gtgtgtgcag caagacaatg agcagttagc gcgatctggt
     4501 acaaactgtt tagagaatgt tgttattctg aatggtgaaa aatttaccct agaaatctgg
     4561 gataaaactt gcaactgcac actggatatc ttcaaaacca caatcccaca tgcgctgttg
     4621 acctggcgac ccaattctgg agaaactgcc cccccacctc catctcctgt aagtgaaaag
     4681 ccattggata caatatcaca gaagtctgta gatattcatg attctattca accaaggtct
     4741 gtggataaca gaccacaagc accactggtt tctgcgtctg ctgttaatga agaagtcagc
     4801 aaaattaaat ctacagcaaa atttccagaa caaaaattgt ttgctgccct gttgattaaa
     4861 tgtgttgtgc agctggaact catccagact atcgacaaca ttgtcttctt cccagccaca
     4921 agtaagaaag aagatgcaga aaacttagct gcagcacaga gagatgcggt ggactttgat
     4981 gttcgcgttg atactcaaga ccaaggaatg taccgctttt taacatcaca acaacttttt
     5041 aagctactgg actgcttatt agagtcacat agatttgcaa aagcgtttaa ttccaacaac
     5101 gaacagagga ctgccctgtg gaaagcagga ttcaaaggca aatccaagcc caaccttctg
     5161 aagcaggaga ccagcagcct ggcctgtggg ctgcgcattc tcttccggat gtacatggat
     5221 gagagccgcg ttagtgcctg ggaggaggtc cagcagaggc ttttgaatgt ctgcagtgaa
     5281 gcactaagtt acttcctcac tctaacatca gaaagtcatc gagaagcctg gactaactta
     5341 ctgcttttgt ttctaactaa agttctaaag ataagtgata ataggtttaa agctcatgca
     5401 tcattctact accctctctt atgtgaaatt atgcaatttg acttgattcc tgaacttcgt
     5461 gctgttctta gaagattttt tctgcgaatc ggagtagttt ttcagatatc acaaccacct
     5521 gaacaggaac ttggaataaa caagcaatga
//
