LOCUS       sequence-180710-        7386 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses AimR gene from the B. subtilis phage phi3T, with its 
            native promoter, C' His-tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7386)
  AUTHORS   Erez Z, Steinberger-Levy I, Shamir M, Doron S, Stokar-Avihail A, 
            Peleg Y, Melamed S, Leavitt A, Savidor A, Albeck S, Amitai G, Sorek 
            R
  TITLE     Communication between viruses guides lysis-lysogeny decisions.
  JOURNAL   Nature. 2017 Jan 26;541(7638):488-493. doi: 10.1038/nature21049. 
            Epub 2017 Jan 18.
  PUBMED    28099413
REFERENCE   2  (bases 1 to 7386)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature21049"; journalName: "Nature"; date: "2017-01-26- 
            26"; volume: "541"; issue: "7638"; pages: "488-493"
COMMENT     Sequence Label: pAimR
FEATURES             Location/Qualifiers
     source          1..7386
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1145..1164)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     rep_origin      complement(3582..4170)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3662..3681)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4341..5132,5133..5201))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4964..4983
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5202..5306)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5374..5392
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             7361..7378
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gtccggcaaa aaagggcaag gtgtcaattc tcatgtttga cagcttatca tcggcaatag
       61 ttacccttat tatcaagata agaaagaaaa ggatttttcg ctacgctcaa atcctttaaa
      121 aaaacacaaa agaccacatt ttttaatgtg gtctttattc ttcaactaaa gcacccatta
      181 gttcaacaaa cgaaaattgg ataaagtggg atatttttaa aatatatatt tatgttacag
      241 taatattgac ttttaaaaaa ggattgattc taatgaagaa agcagacaag taagcctcct
      301 aaattcactt tagataaaaa tttaggaggc atatcaaatg aactttaata aaattgattt
      361 agacaattgg aagagaaaag agatatttaa tcattatttg aaccaacaaa cgacttttag
      421 tataaccaca gaaattgata ttagtgtttt ataccgaaac ataaaacaag aaggatataa
      481 attttaccct gcatttattt tcttagtgac aagggtgata aactcaaata cagcttttag
      541 aactggttac aatagcgacg gagagttagg ttattgggat aagttagagc cactttatac
      601 aatttttgat ggtgtatcta aaacattctc tggtatttgg actcctgtaa agaatgactt
      661 caaagagttt tatgatttat acctttctga tgtagagaaa tataatggtt cggggaaatt
      721 gtttcccaaa acacctatac ctgaaaatgc tttttctctt tctattattc catggacttc
      781 atttactggg tttaacttaa atatcaataa taatagtaat taccttctac ccattattac
      841 agcaggaaaa ttcattaata aaggtaattc aatatattta ccgctatctt tacaggtaca
      901 tcattctgtt tgtgatggtt atcatgcagg attgtttatg aactctattc aggaattgtc
      961 agataggcct aatgactggc ttttataata tgagataatg ccgactgtac tttttacagt
     1021 cggttttcta atgtcactaa cctgccccgt tagttgaaga aggtttttat attacagctc
     1081 cagatcctct acgccggacg catcgtggca ggcatcaccg gcgccacagg tgcggttgct
     1141 ggcgcctata tcgccgacat caccgatggg gaagatcggg ctcgccactt cgggctcatg
     1201 agcgcttgtt tcggcgtggg tatggtggca ggccccgtgg ccgggggact gttgggcgcc
     1261 atctccttgc atgcaccatt ccttgcggcg gcggtgctca acggcctcaa cctactactg
     1321 ggctgcttcc taatgcagga gtcgcataag ggagagcgtc gacatggatg agcgatgatg
     1381 atatccgttt aggctgggcg gtgatagctt ctcgttcagg cagtacgcct cttttctttt
     1441 ccagacctga gggaggcgga aatggtgtga ggttcccggg gaaaagccaa ataggcgatc
     1501 gcgggagtgc tttatttgaa gatcaggcta tcactgcggt caatagattt cacaatgtga
     1561 tggctggaca gcctgaggaa ctctcgaacc cgaatggaaa caaccagata tttatgaatc
     1621 agcgcggctc acatggcgtt gtgctggcaa atgcaggttc atcctctgtc tctatcaata
     1681 cggcaacaaa attgcctgat ggcaggtatg acaataaagc tggagcgggt tcatttcaag
     1741 tgaacgatgg taaactgaca ggcacgatca atgccaggtc tgtagctgtg ctttatcctg
     1801 atgatattgc aaaagcgcct catgttttcc ttgagaatta caaaacaggt gtaacacatt
     1861 ctttcaatga tcaactgacg attaccttgc gtgcagatgc gaatacaaca aaagccgttt
     1921 atcaaatcaa taatggacca gacgacaggc gtttaaggat ggagatcaat tcacaatcgg
     1981 aaaaggagat ccaatttggc aaaacataca ccatcatgtt aaaaggaacg aacagtgatg
     2041 gtgtaacgag gaccgagaaa tacagttttg ttaaaagaga tccagcgtcg gccaaaacca
     2101 tcggctatca aaatccgaat cattggagcc aggtaaatgc ttatatctat aaacatgatg
     2161 ggagccgagt aattgaattg accggatctt ggcctggaaa accaatgact aaaaatgcag
     2221 acggaattta cacgctgacg ctgcctgcgg acacggatac aaccaacgca aaagtgattt
     2281 ttaataatgg cagcgcccaa gtgcccggtc agaatcagcc tggctttgat tacgtgctaa
     2341 atggtttata taatgactcg ggcttaagcg gttctcttcc ccattgaggg caaggctaga
     2401 cgggacttac cgaaagaaac catcaatgat ggtttctttt ttgttcataa atcagacaaa
     2461 acttttctct tgcaaaagtt tgtgaagtgt tgcacaatat aaatgtgaaa tacttcacaa
     2521 acaaaaagac atcaaagaga aacataccct gcaaggatgc tgatattgtc tgcatttgcg
     2581 ccggagcaaa ccaaaaacct ggtgagacac gccttgaatt agtagaaaag aacttgaaga
     2641 ttttcaaagg catcgttagt gaagtcatgg cgagcggatt tgacggcatt ttcttagtcg
     2701 cgacgcgagg ctggatggcc ttccccatta tgattcttct cgcttccggc ggcatcggga
     2761 tgcccgcgtt gcaggccatg ctgtccaggc aggtagatga cgaccatcag ggacagcttc
     2821 aaggatcgct cgcggctctt accagcctaa cttcgatcac tggaccgctg atcgtcacgg
     2881 cgatttatgc cgcctcggcg agcacatgga acgggttggc atggattgta ggcgccgccc
     2941 tataccttgt ctgcctcccc gcgttgcgtc gcggtgcatg gagccgggcc acctactgaa
     3001 gtggatttct ttaagagctc ctttaacttc ctcaccagta gttgtatcgg taccataagt
     3061 agaagcagca acccaagtag ctttaccagc atccggttca accagcatag taagaatctt
     3121 actggacatc ggcagttctt cgaacagtgc gccaactacc agctctttct ccagaatggg
     3181 ctatacctct tttacctaaa gagcatgtaa ctttactgga tatagctaga aaaggctatc
     3241 ggggagagtg tgatgataag tgggaaggac tatattcaaa ggtgaaagca ctcgttaagt
     3301 atatgaaaaa ttctatagaa acttctctca attaggctaa ttttattgca ataacaggtg
     3361 cttacttttc tggagttctt tagcaaattt ttttattagc tgaacttagt attagtggcc
     3421 atactcctcc aatccaaagc tatttagaaa gattactata tcctcaaaca ggcggtaacc
     3481 ggcctcttca tcgggaatgc gcgcgacctt cagcatcacc ggcatgtccc cctggcggac
     3541 gggaagtatc cagctcgagg tcgggccgcg ttgctggcgt ttttccatag gctccgcccc
     3601 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     3661 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     3721 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     3781 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     3841 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     3901 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     3961 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     4021 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttgat
     4081 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     4141 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     4201 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     4261 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     4321 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     4381 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     4441 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     4501 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     4561 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     4621 ccagttaata gtttgcgcaa cgttgttgcc attgctgctg gcatcgtggt gtcacgctcg
     4681 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     4741 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     4801 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     4861 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     4921 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaacac gggataatac cgcgccacat
     4981 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     5041 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     5101 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     5161 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     5221 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     5281 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa
     5341 gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt
     5401 cttcaagaat taacaaaatt ctccagtctt cacatcggtt tgaaaggagg aagcggaaga
     5461 atgaagtaag agggattttt gactccgaag taagtcttca aaaaatcaaa taaggagtgt
     5521 caagaatgtt tgcaaaacga ttcaaaacct ctttactgcc gttattcgct ggatttttat
     5581 tgctgtttca tttggttctg gcaggaccgg cggctgcgag tgctgaaacg gcgaacaaat
     5641 cgaatgagct tacagcaccg tcgatcaaaa gcggaaccat tcttcatgca tggaattggt
     5701 cgttcaatac gttaaaacac aatatgaagg atattcatga tgcaggatat acagccattc
     5761 agacatctcc gattaaccaa gtaaaggaag ggaatcaagg agataaaagc atgtcgaact
     5821 ggtactggct gtatcagccg acatcgtatc aaattggcaa ccgttactta ggtactgaac
     5881 aagaatttaa agaaatgtgt gcagccgctg aagaatatgg cataaaggtc attgttgacg
     5941 cggtcatcaa tcataccacc agtgattatg ccgcgatttc caatgaggtt aagagtattc
     6001 caaactggac acatggaaac acacaaatta aaaactggtc tgatcggatc ctagaagctt
     6061 atcgaattcc tcattgtgtt taggtaaaat aagaaattca ttgatttaac aacaaataaa
     6121 gaataaaaat aaaatatata cttgaaattc tgacaactat gaggtatatt ataaacaagg
     6181 gaagtgataa ctgaataaag gggggagaca attgaaatta aagcagatga ttaagaatga
     6241 atgcgaaaag gataatcagc ttgcagctcg acttgcaaaa ttggccggtt atgaaaaagt
     6301 aaatggtttt tataagtttg tgaacacccc agaaaaagaa atggaaaact tgggtggatt
     6361 actcaagatc gtaaaaaact tgtttcctga tagtgaagag caacttttaa gtgaatactt
     6421 cttagaatta gaccctaata aaaaatgtgc aaggcaatca gttgagtact cagatataaa
     6481 ccaatgggac acacttactg ataagattat cattaactta tgcaactcaa aaaattccac
     6541 aagtcaagag tggggaaaag tttacagctt acatagaaaa ttaaacaaaa acgaaatcag
     6601 tttaaatgat gctattaggg aatcagggaa atgtaaaata aaatccgcgg aaatgctctt
     6661 cttttcaaat gcaatgctga tgtatgcgta tttaaacatt ggtgaatttg gattaatgaa
     6721 gagcacttca aaattgttag aatttgatga tttacccgaa gggttcatta aagagtcatt
     6781 caaaagcaga gtatctatgc tcgaagcgaa cataagctta aatgaaaata gcctacttga
     6841 agcgagacag cattctaacc gcgcaattga aaattctaac gtgaatcgta tttgtttttt
     6901 tgcatattta acaattggca acactttaat ttttgaggat tatgatgagg ccaaaaaggc
     6961 gtacattaaa ggtcaaaaat atgctaaaaa tccagtgcac caagaaatgc ttgatggtgc
     7021 gttgtgcttt ttgtcaaaca tctggaaaaa agaaaatcaa tgggtgaatt ataactctga
     7081 taacattaaa tatttgcaat taagagcttt ttattacata aatcaaggta acattgagga
     7141 agccacggaa attttagatg aactgtcatc aagagatcaa gatgagaatg aattaggatt
     7201 ctactattac tacaaaggat taatatctca ggataagaca gactattata aatcaataag
     7261 atatttcaaa aaatcagatg ataaatattt tatacaattg ccattacttc aactcgaacg
     7321 aatgggggct gatcttgaat tattaaacct tatctctatt caccaccacc accaccacta
     7381 actgca
//
