LOCUS       sequence-180714-       10562 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A control plasmid containing the intact bsdMX marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10562)
  AUTHORS   Zhang XR, He JB, Wang YZ, Du LL
  TITLE     A Cloning-Free Method for CRISPR/Cas9-Mediated Genome Editing in 
            Fission Yeast.
  JOURNAL   G3 (Bethesda). 2018 Apr 27. pii: g3.118.200164. doi: 
            10.1534/g3.118.200164.
  PUBMED    29703785
REFERENCE   2  (bases 1 to 10562)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2018 Apr 27. pii: g3.118.200164. doi: 
            10.1534/g3.118.200164."
COMMENT     Sequence Label: pDB4279
FEATURES             Location/Qualifiers
     source          1..10562
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      539..1320
                     /label=ars1
                     /note="Schizosaccharomyces pombe autonomously replicating 
                     sequence ars1"
     promoter        1398..1741
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     terminator      2140..2337
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     2528..2550
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2542..2559
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2543..2559
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_RNA        complement(2609..2660)
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
     promoter        complement(2779..3135)
                     /label=rrk1 promoter/leader
                     /note="promoter and leader RNA for the Schizosaccharomyces 
                     pombe rrk1 gene encoding the K-RNA component of RNase P 
                     (Jacobs et al., 2014)"
     primer_bind     complement(3172..3188)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3172..3188)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3185..3207)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3196..3212
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3220..3226,3227..3244,3245..3250))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3265..3286
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3403..3420)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3574..4162)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3654..3673)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4333..5124,5125..5193))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4956..4975
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5194..5298)
                     /gene="bla"
                     /label=AmpR promoter
     promoter        5327..6068
                     /label=adh1 promoter
                     /note="promoter for the Schizosaccharomyces pombe adh1 gene
                     encoding alcohol dehydrogenase"
     CDS             6089..10192
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             6089..10192
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             6089..10192
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             10205..10225
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      10241..10488
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(10257..10275)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     10535..10553
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataatcg atggccaacc ttccaattca ttaaatcgag tcctccatca gatgatttac
      241 aaggttttgc atagaatccc taagtaagag aagttgttag tgatttcttc aagtttacta
      301 aaatttacat acaaatggat gatctcttct ccattgtttc ctttcttctt gcaaacgcgt
      361 tttacaaaga gatgacatgc tattttctga ttattttttt ctatagtttt ctattttgta
      421 cgtccttggg agtccgaaat gtaaaatcgg ggtatggtta gtagcggcag cgttcattat
      481 ggtatataaa agataaaaag taggggcaag cgagacagaa tgggatacaa gggcatcgtc
      541 tataattata gctaaaaatt gtattttaat ttgtattttt tgtaatttta tttctcattg
      601 ttttacttaa aatgaatgcg aattagagaa aacttcaacg aaatgtcaaa ataagctcag
      661 caaaatatac aattttaggg aaagcgatca gcaacttatt ctccgttgta gattcttttt
      721 cttcataaat tccaacaatg taactgccaa ttgatttttc tctagatgct aataaattga
      781 aactaatgaa tataataaca caggttttga taaattgtaa actcttactc aaaaaaagaa
      841 tgttaaaact aatttaacct ttttataaag gctcatggcg ttaaagagga tagtaaaaat
      901 tctacaattt tttaatttaa tgtcacaatt gtcttgcgaa tataattcta ttaattttcg
      961 ctaactgctg ttttagtact attactttca atatgcctta tatgcaactt taattcatga
     1021 agaatgcaat atttttgcaa tctttcttgt actgagcgct ttttatcatt tagtactcat
     1081 tatttaattt ttcagtaaag ggcaataaat aaatttgtag aagatgcaat gtaatctcct
     1141 ctatcctttt gctcatatgt ttatgagtat acctagtcta gaaaggcttg tatttaaaat
     1201 attattcaat aaaaattcac aatttttacg acatgtgcta ccattcactt aacttcctga
     1261 ttataaaatt ggttcgttta tactaattac ttaagtacct ttaactaaac aaaatgccta
     1321 tatatatatt aatttacaat gagtgtcaga taagtcacta tgtccgagtg gttaaggagt
     1381 tagactcgcc atcgatggac atggaggccc agaataccct ccttgacagt cttgacgtgc
     1441 gcagctcagg ggcatgatgt gactgtcgcc cgtacattta gcccatacat ccccatgtat
     1501 aatcatttgc atccatacat tttgatggcc gcacggcgcg aagcaaaaat tacggctcct
     1561 cgctgcagac ctgcgagcag ggaaacgctc ccctcacaga cgcgttgaat tgtccccacg
     1621 ccgcgcccct gtagagaaat ataaaaggtt aggatttgcc actgaggttc ttctttcata
     1681 tacttccttt taaaatcttg ctaggataca gttctcacat cacatccgaa cataaacaac
     1741 catgcctttg tctcaagaag aatccaccct cattgaaaga gcaacggcta caatcaacag
     1801 catccccatc tctgaagact acagcgtcgc cagcgcagct ctctctagcg acggccgcat
     1861 cttcactggt gtcaatgtat atcattttac tgggggacct tgtgcagaac tcgtggtgct
     1921 gggcactgct gctgctgcgg cagctggcaa cctgacttgt atcgtcgcga tcggaaatga
     1981 gaacaggggc atcttgagcc cctgcggacg gtgtcgacag gtgcttctcg atctgcatcc
     2041 tgggatcaaa gccatagtga aggacagtga tggacagccg acggcagttg ggattcgtga
     2101 attgctgccc tctggttatg tgtgggaggg ctaatcagta ctgacaataa aaagattctt
     2161 gttttcaaga acttgtcatt tgtatagttt ttttatattg tagttgttct attttaatca
     2221 aatgttagcg tgatttatat tttttttcgc ctcgacatca tctgcccaga tgcgaagtta
     2281 agtgcgcaga aagtaatatc atgcgtcaat cgtatgtgaa tgctggtcgc tatactgcat
     2341 cgcgatggta tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg
     2401 cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg
     2461 ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca
     2521 gggttttccc agtcacgacg ttgtaaaacg acggccagtg ccaagcttgc atgcctgcag
     2581 gtcgactcta gaggatcccc acggtacccc tgtttcgtcc tcacggactc atcagaccgg
     2641 aaagcacatc cggtgacagg gcaccaccga ctcggtgcca ctcacttttt tcaagttgat
     2701 aacggactag ccttatttta acttgctatt tctagctcta aaacagagag ataacccaca
     2761 agaattgagt taagcccatt cttcggtaca ggttatgttt tttggcaaca gcaactattc
     2821 aaatcaactg aaagtttttt agaattcaac aacttccaat actcaagtgt ccaagctagc
     2881 aaactgttta ataatcgtga cccaacattc ctttcatgcg caccctcagc gtcacataaa
     2941 tcaacacttt acaacaccta ttcaaagctt ctatatgcct tcgtcattta aatgtctaac
     3001 atttcaaaaa agtgtttgct aatggtaaat tcttgagttt tcttggaagc acatatataa
     3061 cgagtgtgac tgttctacgc tccttgctga ctagtcatca gtctacgcat gccaactacc
     3121 accaacataa gcaaaaggta cccggggtac cgagctcgaa ttcgtaatca tgtcatagct
     3181 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
     3241 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
     3301 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     3361 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     3421 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     3481 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     3541 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     3601 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     3661 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     3721 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     3781 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     3841 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     3901 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     3961 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     4021 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     4081 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     4141 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     4201 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     4261 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     4321 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     4381 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     4441 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     4501 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     4561 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     4621 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     4681 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     4741 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     4801 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     4861 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     4921 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     4981 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     5041 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     5101 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     5161 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     5221 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     5281 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgccc tacaacaact
     5341 aagaaaatgg ctatcatgcg gaagctggtg agaagaacag catcgggaca agggaaggaa
     5401 gaacaaagac aaagaaaaca aaagaaagca attgaaaaca aaacaaaaca attttcattc
     5461 cttctcttat cattcctttt cttttctttt ctctcattca acgcactcca tcgtatccgt
     5521 attcctctta ttttttctct ttctctatat ccatttcttt ctctctaggt gtgtcctctc
     5581 tctctcttca atttctctac tccgcattcc aacgcatcct tcccccaacc tcccatttcc
     5641 tccttacggc ccgatagcga tcgtctttcc ctcgctatca ctcgctaccg gcccctcctc
     5701 tgcaccgtaa cctcctacgt atttaccata tcataaagtt ttttccgacg cttatcgctg
     5761 accccctgtc gccctcctat tggcttccgg attatcttct tgtccataag gtgatccatg
     5821 cttcctgaag attcccgaaa tgtgtccact ttggcgggga atcattccat ccacttcttt
     5881 ctctctcgct ttcctcattc ggcgctcccc ttccgcgtct cattggtctt ccgctccgtt
     5941 tttgctttgc cgatgttact tggggagagg tgcgataatc ctttcgcaaa aactcggttt
     6001 gacgcctccc atggtataaa tagtgggtgg tggacaggtg ccttcgcttt tctttaagca
     6061 agagaattgc tgcaggtcga ctctagagat ggacaagaag tactccattg ggctcgatat
     6121 cggcacaaac agcgtcggtt gggccgtcat tacggacgag tacaaggtgc cgagcaaaaa
     6181 attcaaagtt ctgggcaata ccgatcgcca cagcataaag aagaacctca ttggcgccct
     6241 cctgttcgac tccggggaga cggccgaagc cacgcggctc aaaagaacag cacggcgcag
     6301 atatacccgc agaaagaatc ggatctgcta cctgcaggag atctttagta atgagatggc
     6361 taaggtggat gactctttct tccataggct ggaggagtcc tttttggtgg aggaggataa
     6421 aaagcacgag cgccacccaa tctttggcaa tatcgtggac gaggtggcgt accatgaaaa
     6481 gtacccaacc atatatcatc tgaggaagaa gcttgtagac agtactgata aggctgactt
     6541 gcggttgatc tatctcgcgc tggcgcatat gatcaaattt cggggacact tcctcatcga
     6601 gggggacctg aacccagaca acagcgatgt cgacaaactc tttatccaac tggttcagac
     6661 ttacaatcag cttttcgaag agaacccgat caacgcatcc ggagttgacg ccaaagcaat
     6721 cctgagcgct aggctgtcca aatcccggcg gctcgaaaac ctcatcgcac agctccctgg
     6781 ggagaagaag aacggcctgt ttggtaatct tatcgccctg tcactcgggc tgacccccaa
     6841 ctttaaatct aacttcgacc tggccgaaga tgccaagctt caactgagca aagacaccta
     6901 cgatgatgat ctcgacaatc tgctggccca gatcggcgac cagtacgcag accttttttt
     6961 ggcggcaaag aacctgtcag acgccattct gctgagtgat attctgcgag tgaacacgga
     7021 gatcaccaaa gctccgctga gcgctagtat gatcaagcgc tatgatgagc accaccaaga
     7081 cttgactttg ctgaaggccc ttgtcagaca gcaactgcct gagaagtaca aggaaatttt
     7141 cttcgatcag tctaaaaatg gctacgccgg atacattgac ggcggagcaa gccaggagga
     7201 attttacaaa tttattaagc ccatcttgga aaaaatggac ggcaccgagg agctgctggt
     7261 aaagcttaac agagaagatc tgttgcgcaa acagcgcact ttcgacaatg gaagcatccc
     7321 ccaccagatt cacctgggcg aactgcacgc tatcctcagg cggcaagagg atttctaccc
     7381 ctttttgaaa gataacaggg aaaagattga gaaaatcctc acatttcgga taccctacta
     7441 tgtaggcccc ctcgcccggg gaaattccag attcgcgtgg atgactcgca aatcagaaga
     7501 gaccatcact ccctggaact tcgaggaagt cgtggataag ggggcctctg cccagtcctt
     7561 catcgaaagg atgactaact ttgataaaaa tctgcctaac gaaaaggtgc ttcctaaaca
     7621 ctctctgctg tacgagtact tcacagttta taacgagctc accaaggtca aatacgtcac
     7681 agaagggatg agaaagccag cattcctgtc tggagagcag aagaaagcta tcgtggacct
     7741 cctcttcaag acgaaccgga aagttaccgt gaaacagctc aaagaagact atttcaaaaa
     7801 gattgaatgt ttcgactctg ttgaaatcag cggagtggag gatcgcttca acgcatccct
     7861 gggaacgtat cacgatctcc tgaaaatcat taaagacaag gacttcctgg acaatgagga
     7921 gaacgaggac attcttgagg acattgtcct cacccttacg ttgtttgaag atagggagat
     7981 gattgaagaa cgcttgaaaa cttacgctca tctcttcgac gacaaagtca tgaaacagct
     8041 caagaggcgc cgatatacag gatgggggcg gctgtcaaga aaactgatca atgggatccg
     8101 agataagcag tctggaaaga caatcctgga ttttcttaag tccgatggat ttgccaaccg
     8161 gaacttcatg cagttgatcc atgatgactc tctcaccttt aaggaggaca tccagaaagc
     8221 acaagtttct ggccaggggg acagtcttca cgagcacatc gctaatcttg caggtagccc
     8281 agctatcaaa aagggaatac tgcagaccgt taaggtcgtg gatgaactcg tcaaagtaat
     8341 gggaaggcat aagcccgaga atatcgttat cgagatggcc cgagagaacc aaactaccca
     8401 gaagggacag aagaacagta gggaaaggat gaagaggatt gaagagggta taaaagaact
     8461 ggggtcccaa atccttaagg aacacccagt tgaaaacacc cagcttcaga atgagaagct
     8521 ctacctgtac tacctgcaga acggcaggga catgtacgtg gatcaggaac tggacatcaa
     8581 tcggctctcc gactacgacg tggatcatat cgtgccccag tcttttctca aagatgattc
     8641 tattgataat aaagtgttga caagatccga taaaaataga gggaagagtg ataacgtccc
     8701 ctcagaagaa gttgtcaaga aaatgaaaaa ttattggcgg cagctgctga acgccaaact
     8761 gatcacacaa cggaagttcg ataatctgac taaggctgaa cgaggtggcc tgtctgagtt
     8821 ggataaagcc ggcttcatca aaaggcagct tgttgagaca cgccagatca ccaagcacgt
     8881 ggcccaaatt ctcgattcac gcatgaacac caagtacgat gaaaatgaca aactgattcg
     8941 agaggtgaaa gttattactc tgaagtctaa gctggtctca gatttcagaa aggactttca
     9001 gttttataag gtgagagaga tcaacaatta ccaccatgcg catgatgcct acctgaatgc
     9061 agtggtaggc actgcactta tcaaaaaata tcccaagctt gaatctgaat ttgtttacgg
     9121 agactataaa gtgtacgatg ttaggaaaat gatcgcaaag tctgagcagg aaataggcaa
     9181 ggccaccgct aagtacttct tttacagcaa tattatgaat tttttcaaga ccgagattac
     9241 actggccaat ggagagattc ggaagcgacc acttatcgaa acaaacggag aaacaggaga
     9301 aatcgtgtgg gacaagggta gggatttcgc gacagtccgg aaggtcctgt ccatgccgca
     9361 ggtgaacatc gttaaaaaga ccgaagtaca gaccggaggc ttctccaagg aaagtatcct
     9421 cccgaaaagg aacagcgaca agctgatcgc acgcaaaaaa gattgggacc ccaagaaata
     9481 cggcggattc gattctccta cagtcgctta cagtgtactg gttgtggcca aagtggagaa
     9541 agggaagtct aaaaaactca aaagcgtcaa ggaactgctg ggcatcacaa tcatggagcg
     9601 atcaagcttc gaaaaaaacc ccatcgactt tctcgaggcg aaaggatata aagaggtcaa
     9661 aaaagacctc atcattaagc ttcccaagta ctctctcttt gagcttgaaa acggccggaa
     9721 acgaatgctc gctagtgcgg gcgagctgca gaaaggtaac gagctggcac tgccctctaa
     9781 atacgttaat ttcttgtatc tggccagcca ctatgaaaag ctcaaagggt ctcccgaaga
     9841 taatgagcag aagcagctgt tcgtggaaca acacaaacac taccttgatg agatcatcga
     9901 gcaaataagc gaattctcca aaagagtgat cctcgccgac gctaacctcg ataaggtgct
     9961 ttctgcttac aataagcaca gggataagcc catcagggag caggcagaaa acattatcca
    10021 cttgtttact ctgaccaact tgggcgcgcc tgcagccttc aagtacttcg acaccaccat
    10081 agacagaaag cggtacacct ctacaaagga ggtcctggac gccacactga ttcatcagtc
    10141 aattacgggg ctctatgaaa caagaatcga cctctctcag ctcggtggag acagcagggc
    10201 tgaccccaag aagaagagga aggtgtgatc tcttctcgag tcatgtaatt agttatgtca
    10261 cgcttacatt cacgccctcc ccccacatcc gctctaaccg aaaaggaagg agttagacaa
    10321 cctgaagtct aggtccctat ttattttttt atagttatgt tagtattaag aacgttattt
    10381 atatttcaaa tttttctttt ttttctgtac agacgcgtgt acgcatgtaa cattatactg
    10441 aaaaccttgc ttgagaaggt tttgggacgc tcgaaggctt taatttgcga tccccgtcta
    10501 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg
    10561 tc
//
