LOCUS       sequence-18073-e         658 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 658)
  TITLE     PTEN
REFERENCE   2  (bases 1 to 658)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: 830 pcDNA3 GFP PTEN 1-336
FEATURES             Location/Qualifiers
     source          1..658
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(104..132)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagcttgcc accatgagta aaggagaaga actcttcact ggagttgtcc caattcttgt
      121 tgaattagat ggtgatgtta atgggcacaa attttctgtc agtggagagg gtgaaggtga
      181 tgcaacatac ggaaaactta cccttaaatt tatttgcact actggaaaac tacctgttcc
      241 atggccaaca cttgtcacta ctttcactta tggtgttcag tgcttttcaa gatacccgga
      301 tcatatgaaa cggcatgact ttttcaagag tgccatgccc gaaggttatg tacaggaaag
      361 aactatattt ttcaaagatg acgggaacta caagacacgt gctgaagtca agtttgaagg
      421 tgataccctt gttaatagaa tcgagttaaa aggtattgat tttaaagaag atggaaacat
      481 tcttgggcac aaattggaat acaactataa ctcacacaat gtatacatca tggcagacaa
      541 acaaaagaat ggaatcaaag ctaacttcaa aattaaacac aacattgaag atggaagcgt
      601 tcaactagca gaccattatc aacaaaatac tccaattggc gatggccctg tcctttta
//
