LOCUS       sequence-180744-        7919 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses mCherry under control of GAL1 promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7919)
  AUTHORS   Aranda-Diaz A, Mace K, Zuleta I, Harrigan P, El-Samad H
  TITLE     Robust Synthetic Circuits for Two-Dimensional Control of Gene 
            Expression in Yeast.
  JOURNAL   ACS Synth Biol. 2016 Dec 27. doi: 10.1021/acssynbio.6b00251.
  PUBMED    27930885
REFERENCE   2  (bases 1 to 7919)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 Dec 27. doi: 10.1021/acssynbio.6b00251."
COMMENT     Sequence Label: pHES875
FEATURES             Location/Qualifiers
     source          1..7919
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        2171..2612
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     protein_bind    2171..2288
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     2583..2606
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     primer_bind     complement(2775..2793)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3043..3062
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(3324..3340)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3324..3340)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(4475..4494)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4476..4494)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4501..4518)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4501..4517)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4510..4532)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4658..5113
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4745..4764)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4955..4976
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5469..5488)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5588..5610
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5648..5666)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5751..5855
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5856..5924,5925..6716)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6074..6093)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6887..7475
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7376..7395
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7629..7646
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7763..7784
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7799..7804,7805..7822,7823..7829)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7837..7853
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7842..7864
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7861..7877
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7861..7877
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        7898..7916
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 caaaagctgg gccggccatt taaatgttta aacggccacg ctggccatat ttctaccaat
       61 ctctcaacac tgagtaatgg tagttataag aaagagaccg agttagggac agttagaggc
      121 ggtggagata ttccttatgg catgtctggc gatgataaaa cttttcaaac ggcagccccg
      181 atctaaaaga gctgacaggg aaatggtcag aaaaagaaac gtgcacccgc ccgtctggac
      241 gcgccgctca cccgcacggc agagaccaat cagtaaaaat caacggttaa cgacattact
      301 atatatataa tataggaagc atttaataga acagcatcgt aatatatgtg tactttgcag
      361 ttatgacgcc agatggcagt agtggaagat attctttatt gaaaaatagc ttgtcacctt
      421 acgtacaatc ttgatccgga gcccgtcgct ttgagagaaa tcagaagatt ccaaaagtct
      481 actgaacttt tgatcagaaa gttgcctttc caaagactag tcagagaaat cgcccaagat
      541 ttcaagaccg atctaagatt ccaatcctct gccatcggtg ctctacagga atccgttgaa
      601 gcttacttgg tctctttgtt cgaagacacc aacttggccg ctatccacgc taagcgtgtt
      661 accatccaaa agaaggatat caagttggct agaagattga gaggtgaaag atcctaagca
      721 cacagggatt gttgtacaat tactttttat gattttagat acacacaaat cttttctacg
      781 attacctttc atttattttc gtttcatgtt ttattaatca ttctttttgt atatttcaca
      841 tcgggtgggg tttttatatt aaattttaat aacataatct tttaaagttt ctactttcta
      901 tcctgtatat tatttccata tctatatcta tattttcaag cttgttcaca tccttatcgg
      961 aattgttcca gcgtttttgt acagaaaatg atgttatggt aacgagctat atcagaccta
     1021 ctagtcttat agagctgtac agagtcttgg aaacataacc aacacaccaa caagatgtca
     1081 tttgattcgt tactcgacaa gaatgataag ctggtaaaag tttgcgggat tcaaaccgtc
     1141 gaggctgccg aaactgcgct tcaagcaggc gctgatttga tagggatcat atgtgtcccc
     1201 aacaggaagc ggactatcga gagcgctgtg gctcgtgaaa tatccaaatt gattcacaaa
     1261 tcaggtacta caaagctggt gggggtgttc aggaatcaat ctgttgagga cgtacatcgg
     1321 ctttctgagg aatatgacct tgacataatc caattacatg gtgatgaatc atggccagag
     1381 tactataacg tcattaagaa accaataatc aaaagagtca tattccctag agatgtcgat
     1441 gttgtaacac aagtgtgtca aagaaaaccc ttggtatgtc taccattgtt cgactccgag
     1501 gcaggcggta caggtgaaaa gctggactgg tccagtattt cctcctgggc aagcgaacag
     1561 aataacgtat tttatatact agcgggtgga ctaactgcag aaaacgtcat ggaggcggtg
     1621 gggttgcctg gtgtaattgg tgttgacgtc tcaggtggtg tagagactga cggcgtcaaa
     1681 gacaatgaca agataattaa atatgtacaa aatgcaaaga aacaatgaat tacgaagaga
     1741 agtctcaatg aggttttaca aacaatccta taatacatat atgccactta gatatacttt
     1801 gaatactgaa tatactgact cattatattc tttaattgta gtttctcaaa aatattcata
     1861 caggttagct atgaattcat gctataattt ccattttgtt catgtaagat acacataggg
     1921 ataaaatgtg ataactaatc agcggtaccg ggcccttata ttgaattttc aaaaattctt
     1981 actttttttt tggatggacg caaagaagtt taataatcat attacatggc attaccacca
     2041 tatacatatc catatctaat cttacttata tgttgtggaa atgtaaagag ccccattatc
     2101 ttagcctaaa aaaaccctct ctttggaact ttcagtaata cgcttaactg ctcattgcta
     2161 tattgaagta cggattagaa gccgccgagc gggcgacagc cctccgacgg aagactctcc
     2221 tccgtgcgtc ctcgtcttca ccggtcgcgt tcctgaaacg cagatgtgcc tcgcgccgca
     2281 ctgctccgaa caataaagat tctacaatac tagcttttat ggttatgaag aggaaaaatt
     2341 agcagtaacc tggccccaca aaccttcaaa ttaacgaatc aaattaacaa ccataggatg
     2401 ataatgcgat tagtttttta gccttatttc tggggtaatt aatcagcgaa gcgatgattt
     2461 ttgatctatt aacagatata taaatggaaa agctgcataa ccactttaac taatactttc
     2521 aacattttca gtttgtatta cttcttattc aaatgtcata aaagtatcaa caaaaaattg
     2581 ttaatatacc tctatacttt aacgtcaagg agaaaaaact atactcgaga tggtgagcaa
     2641 gggcgaggag gataacatgg ccatcatcaa ggagttcatg agattcaagg tgcacatgga
     2701 gggctccgtg aacggccacg agttcgagat cgagggcgag ggcgagggca gaccctacga
     2761 gggcacccag accgccaagc tgaaggtgac caagggtggc cccctgccct tcgcctggga
     2821 catcctgtcc cctcagttca tgtacggctc caaggcctac gtgaagcacc ccgccgacat
     2881 ccctgactac ttgaagctgt ccttccccga gggcttcaag tgggagagag tgatgaactt
     2941 cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg acgagttcat
     3001 ctacaaggtg aagttgagag gcaccaactt cccctccgac ggccccgtaa tgcagaagaa
     3061 gaccatgggc tgggaggcct cctccgagag aatgtacccc gaggacggcg ccctgaaggg
     3121 cgagatcaag cagaggctga agctgaagga cggcggccac tacgacgctg aggtcaagac
     3181 cacctacaag gccaagaagc ccgtgcagct gcccggcgcc tacaacgtca acatcaagtt
     3241 ggacatcacc tcccacaacg aggactacac cattgtggaa caatatgaaa gagctgaagg
     3301 tagacattct actggtgctt agggatccac tagttctaga cacctgcaac atgcgtgagt
     3361 aaataagcgg ccgccaccgc ggtggagctc taagcaaata gctaaattat atacgaatta
     3421 atattatgat taagtgttta cgtgagtgcg atatttttat tactatctta tacagttgta
     3481 tatactctat aaaatgagtt gtctattaat taacgcgatg aatgctttct gggtttacct
     3541 ctccaacaac tctagtttac ttctcaatac attcaattgt atttgatttg tcaatacttc
     3601 atcattaatc aattctatag ttttgttttt ctcgtttatt tccaaattta atgcatcaat
     3661 tttattattc aatttgtcgt tgattttggt taatgatttt atggtttgat ctctggcatt
     3721 gattgtttgt gttagttttt cattattgat aattaaatta tttaagttag ttatcaactc
     3781 ggtgttttca agtttcaagt tttcaatttc tttagagttt attagatttg tcaaagtttc
     3841 tgaattgctt gattggtcct gtagaagagt atttgttgtt gtggataatt gattcaattt
     3901 ttgagacaat tgctggaagg cgttgaaata tctagcatca atctcatggt ttttttcccg
     3961 agagtctcgt agattcaatt gttttaatat atcttgggac cactcttgat ttgaactcat
     4021 ggaaattaaa ctgggtgttg tgttgtggtg taatgattgt accccctttg cttataattg
     4081 tgtggttccg atgctgactt actgggtatt atatgtgtgc ccaatagaaa gagaacaatt
     4141 gacccggtta ttgcaaggaa aatttcaagt cttgtaaaag catataaaaa tagttcaggc
     4201 actccgaaat acttggttgg cgtgtttcgt aatcaaccta aggaggatgt tttggctctg
     4261 gtcaatgatt acggcattga tatcgtccaa ctgcatggag atgagtcgtg gcgagaatac
     4321 caagagttcc tcggtttgcc agttattaaa agactcgtat ttccaaaaga ctgcaacata
     4381 ctactcagtg cagcttcaca gaaacctcat tcgtttattc ccttgtttga ttcagaaggc
     4441 cggccattta aatgtttaaa cggccacgct ggccccctat agtgagtcgt attacaattc
     4501 actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg
     4561 ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg
     4621 cccttcccaa cagttgcgca gcctgaatgg cgaatggacg cgccctgtag cggcgcatta
     4681 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     4741 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     4801 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     4861 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     4921 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     4981 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     5041 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
     5101 acgcttacaa tttcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc
     5161 gcatagggta ataactgata taattaaatt gaagctctaa tttgtgagtt tagtattagg
     5221 atctctaccc ttggcgaaaa gtcctctgcc aacaatgatg atatctgatc caccacttac
     5281 aacttcgtcg acggttctgt actgctgacc caatatgcgt atatatacca atctaagtct
     5341 gtgctccttc cttcgttctt ccttctgttc ggagattacc gaatcaaaaa aatttcaagg
     5401 aaaccgaaat caaaaaaaag aataaaaaaa aaatgatgaa ttgaaaaggt ggtatggtgc
     5461 actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca
     5521 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg
     5581 accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga
     5641 cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct
     5701 tagacgtcgg cgcgccgagg acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     5761 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     5821 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     5881 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     5941 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     6001 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     6061 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     6121 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     6181 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     6241 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     6301 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     6361 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     6421 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     6481 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     6541 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     6601 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     6661 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     6721 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     6781 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     6841 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     6901 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     6961 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     7021 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     7081 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     7141 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     7201 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     7261 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     7321 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     7381 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     7441 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     7501 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     7561 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     7621 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
     7681 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     7741 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
     7801 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
     7861 caggaaacag ctatgaccat gattacgcca agctcggaat taaccctcac taaagggaa
//
