LOCUS       sequence-18075-e         846 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 846)
  TITLE     PTEN
REFERENCE   2  (bases 1 to 846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: 832 pcDNA3 GFP PTEN delta274-342
FEATURES             Location/Qualifiers
     source          1..846
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     27..46
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        27..45
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             65..778
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKA
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(95..123)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     718..738
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 cccactgctt actggcttat cgaaattaat acgactcact atagggagac ccaagcttgc
       61 caccatgagt aaaggagaag aactcttcac tggagttgtc ccaattcttg ttgaattaga
      121 tggtgatgtt aatgggcaca aattttctgt cagtggagag ggtgaaggtg atgcaacata
      181 cggaaaactt acccttaaat ttatttgcac tactggaaaa ctacctgttc catggccaac
      241 acttgtcact actttcactt atggtgttca gtgcttttca agatacccgg atcatatgaa
      301 acggcatgac tttttcaaga gtgccatgcc cgaaggttat gtacaggaaa gaactatatt
      361 tttcaaagat gacgggaact acaagacacg tgctgaagtc aagtttgaag gtgataccct
      421 tgttaataga atcgagttaa aaggtattga ttttaaagaa gatggaaaca ttcttgggca
      481 caaattggaa tacaactata actcacacaa tgtatacatc atggcagaca aacaaaagaa
      541 tggaatcaaa gctaacttca aaattagaca caacattgaa gatggaagcg ttcaactagc
      601 agaccattat caacaaaata ctccaattgg cgatggccct gtccttttac cagacaacca
      661 ttacctgtcc acacaatctg ccctttcgaa agatcccaac gaaaagagag accacatggt
      721 ccttcttgag tttgtaacag ctgctgggat tacacatggc atggatgaac tatacaaaca
      781 gcagcttcag ggatccatga cagccatcat caaagagatc gttagcagaa acaaaaggag
      841 atatca
//
