LOCUS       sequence-18085-e         793 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 793)
  TITLE     PTEN
REFERENCE   2  (bases 1 to 793)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: 892 pFastBacHTb PTEN
FEATURES             Location/Qualifiers
     source          1..793
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             105..122
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             144..164
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 ctgttttcgn nnngttttgt nnaaaaaaac ctataaatat tccggattat tcataccgtc
       61 ccaccatcgg gcgcggatct cggtccgaaa ccatgtcgta ctaccatcac catcaccatc
      121 acgattacga tatcccaacg accgaaaacc tgtattttca gggcgccatg ggatccatga
      181 cagccatcat caaagagatc gttagcagaa acaaaaggag atatcaagag gatggattcg
      241 acttagactt gacctatatt tatccaaaca ttattgctat gggatttcct gcagaaagac
      301 ttgaaggcgt atacaggaac aatattgatg atgtagtaag gtttttggat tcaaagcata
      361 aaaaccatta caagatatac aatctttgtg ctgaaagaca ttatgacacc gccaaattta
      421 attgcagagt tgcacaatat ccttttgaag accataaccc accacagcta gaacttatca
      481 aacccttttg tgaagatctt gaccaatggc taagtgaaga tgacaatcat gttgcagcaa
      541 ttcactgtaa agctggaaag ggacgaactg gtgtaatgat atgtgcatat ttattacatc
      601 ggggcaaatt tttaaaggca caagaggccc tagatttcta tggggaagta aggaccagag
      661 acaaaaaggg agtaactatt cccagtcaga ggcgctatgt gtattattat agctacctgt
      721 taaagaatca tctggattat agaccagtgg cactgttgtt tcanaagatg atgtttgaaa
      781 cnnttccaan ntt
//
