LOCUS       sequence-180878-        1519 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  In vivo visualization of the ER (can be used for colocalization 
            studies and the OSER assay).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1519)
  TITLE     Gadella lab plasmids
REFERENCE   2  (bases 1 to 1519)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: CytERM-mKOkappa
FEATURES             Location/Qualifiers
     source          1..1519
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     enhancer        97..400
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        401..604
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     555..575
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      826..835
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             853..1500
                     /codon_start=1
                     /product="monomeric Kusabira-Orange 2 fluorescent protein"
                     /label=mKO2
                     /translation="SVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRVTM
                     AEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSASV
                     SAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMYLK
                     LEGGGNHKCQFKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAHS"
ORIGIN
        1 cctgattctg tggataaccg tattaccgcc atgcattagt tattaatagt aatcaattac
       61 ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg
      121 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc
      181 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac
      241 tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa
      301 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac
      361 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta
      421 catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga
      481 cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa
      541 ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag
      601 agctggttta gtgaaccgtc agatccgcta gcgctaccgg actcagatct ggtacccctt
      661 gcagcttcca gttgtcagga agagaaggct cccatggatc ctgtggtggt gctggggctc
      721 tgtctctcct gtttgcttct cctttcactc tggaaacaga gctatggggg agggaagctt
      781 cgaattctgc agtcgacggt accgcgggcc cgggatccac cggtcgccac catggtgagc
      841 aagggcgagg agagtgtgat taaaccagag atgaagatga ggtactacat ggacggctcc
      901 gtcaatgggc atgagttcac aattgaaggt gaaggcacag gcagacctta cgagggacat
      961 caagagatga cactacgcgt cacaatggcc gagggcgggc caatgccttt cgcgtttgac
     1021 ttagtgtcac acgtgttctg ttacggccac agagtattta ctaaatatcc agaagagata
     1081 ccagactatt tcaaacaagc atttcctgaa ggcctgtcat gggaaaggtc gttggagttc
     1141 gaagatggtg ggtccgcttc agtcagtgcg catataagcc ttagaggaaa caccttctac
     1201 cacaaatcca aatttactgg ggttaacttt cctgccgatg gtcctatcat gcaaaaccaa
     1261 agtgttgatt gggagccatc aaccgagaaa attactgcca gcgacggagt tctgaagggt
     1321 gatgttacga tgtacctaaa acttgaagga ggcggcaatc acaaatgcca attcaagact
     1381 acttacaagg cggcaaaaga gattcttgaa atgccaggag accattacat cggccatcgc
     1441 ctcgtcagga aaaccgaagg caacattact gagcaggtag aagatgcagt agctcattcc
     1501 actggcgacg agctgtaca
//
