LOCUS       sequence-180887-        5486 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5486)
  AUTHORS   Heckler MM, Thakor H, Schafer CC, Riggins RB
  TITLE     ERK/MAPK regulates ERRgamma expression, transcriptional activity, 
            and receptor-mediated Tamoxifen resistance in ER+ breast cancer.
  JOURNAL   FEBS J. 2014 Mar 29. doi: 10.1111/febs.12797.
  PUBMED    24684682
REFERENCE   2  (bases 1 to 5486)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEBS J. 
            2014 Mar 29. doi: 10.1111/febs.12797."
COMMENT     Sequence Label: pSG5-HA-ERR3 S45A
FEATURES             Location/Qualifiers
     source          1..5486
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..27
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             231..242
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     polyA_signal    1431..1552
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1468..1487)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1522..1541
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1586..1603)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1757..2345)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1837..1856)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2516..3307,3308..3376))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3139..3158
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3377..3481)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3549..3567
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(3605..3627)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3727..3746
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(3813..4268)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3950..3971)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4162..4181
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4394..4416
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4408..4425
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4409..4425
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4462..4482)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4570..4780
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        4592..4788
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      4639..4774
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          4819..5391
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     5399..5418
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     5446..5465
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5446..5464
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      5475..5484
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tacccatacg atgttcctga ctatgcggaa ttctcgagtg attcggtaga actttgcctg
       61 cctgaatctt tttccctgca ctacgaagaa gagcttctct gcagaatgtc aaacaaagat
      121 cgacacattg attccagctg ttcgtccttc atcaagacgg aaccctccgc cccagcctcc
      181 ctgacggaca gcgtcaacca ccacagccct ggtgggtctt ccgacgccag tgggagttac
      241 agttcaacca tgaatggcca tcagaacgga ctggactcgc cacctctcta cccctctgct
      301 ccgatcctgg gaggcagcgg gcctgtccgg aaactgtatg atgactgctc cagcaccatc
      361 gtagaggatc cccagaccaa gtgtgaatat atgctcaact ccatgcccaa gagactgtgc
      421 ttagtgtgtg gcgacatcgc ctctgggtac cactatgggg ttgcatcatg tgaagcctgc
      481 aaggcattct tcaagaggac gattcaaggt aacatagagt acagctgccc agccacgaat
      541 gaatgtgaga tcacaaagcg cagacgcaaa tcctgccagg cctgccgctt catgaagtgt
      601 ctcaaagtgg gcatgctgaa agaaggggtc cgtcttgaca gagtgcgtgg aggtcggcag
      661 aagtacaagc gcagaataga tgctgagaac agcccatacc tgaaccctca gctggtgcag
      721 ccagccaaaa agccatataa caagattgtc tcgcatttgt tggtggctga accagagaag
      781 atctatgcca tgcctgaccc tactgtcccc gacagtgaca tcaaagccct caccacactc
      841 tgtgacttgg ctgaccgaga gttggtggtt atcattggat gggcaaaaca tattccaggc
      901 ttctccacac tgtcccttgc agaccagatg agcctcctcc agagtgcatg gatggagatt
      961 ctgatcctcg gcgttgtgta ccgatcgctt tcgtttgagg atgaacttgt ctatgcagac
     1021 gattatataa tggatgaaga ccagtctaaa ttagcaggcc ttcttgacct aaataatgct
     1081 atcctgcagc tggtgaagaa gtacaagagc atgaagctag agaaggaaga attcgtcacc
     1141 ctcaaagcaa tagctcttgc taattcagat tccatgcata tagaagatgt ggaagctgtg
     1201 cagaaacttc aggatgtgtt acatgaggcc ctgcaggatt acgaggctgg ccagcacatg
     1261 gaagaccctc gccgtgcagg caagatgctg atgacgctgc cgctgctgag gcagacctcc
     1321 accaaggcag tccagcactt ctacaacatc aaactcgaag gcaaagtgcc catgcacaaa
     1381 ctttttttgg aaatgctgga ggccaaggtc tgaggatctt attaaagcag aacttgttta
     1441 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     1501 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     1561 ggtcgactct agactcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     1621 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     1681 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     1741 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     1801 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     1861 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     1921 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     1981 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     2041 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     2101 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     2161 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     2221 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     2281 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     2341 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     2401 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     2461 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     2521 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     2581 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     2641 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     2701 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     2761 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     2821 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     2881 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     2941 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     3001 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     3061 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     3121 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     3181 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     3241 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     3301 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     3361 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     3421 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     3481 gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa
     3541 cctataaaaa taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg
     3601 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg
     3661 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta
     3721 actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc
     3781 acagatgcgt aaggagaaaa taccgcatca ggaaattgta agcgttaata ttttgttaaa
     3841 attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa
     3901 aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa
     3961 caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca
     4021 gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg
     4081 taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc
     4141 ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc
     4201 aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca
     4261 gggcgcgtcc attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc
     4321 tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta
     4381 acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gtcgacttct
     4441 gaggcggaaa gaaccagctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct
     4501 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaagg aaagtcccca
     4561 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
     4621 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     4681 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     4741 ttccagaagt agtgaagagg cttttttgga ggcctaggct tttgcaaaaa gctccggatc
     4801 gatcctgaga acttcagggt gagtttgggg acccttgatt gttctttctt tttcgctatt
     4861 gtaaaattca tgttatatgg agggggcaaa gttttcaggg tgttgtttag aatgggaaga
     4921 tgtcccttgt atcaccatgg accctcatga taattttgtt tctttcactt tctactctgt
     4981 tgacaaccat tgtctcctct tattttcttt tcattttctg taactttttc gttaaacttt
     5041 agcttgcatt tgtaacgaat ttttaaattc acttttgttt atttgtcaga ttgtaagtac
     5101 tttctctaat cacttttttt tcaaggcaat cagggtatat tatattgtac ttcagcacag
     5161 ttttagagaa caattgttat aattaaatga taaggtagaa tatttctgca tataaattct
     5221 ggctggcgtg gaaatattct tattggtaga aacaactaca tcctggtcat catcctgcct
     5281 ttctctttat ggttacaatg atatacactg tttgagatga ggataaaata ctctgagtcc
     5341 aaaccgggcc cctctgctaa ccatgttcat gccttcttct ttttcctaca gctcctgggc
     5401 aacgtgctgg ttattgtgct gtctcatcat tttggcaaag aattgtaata cgactcacta
     5461 tagggcgaat taccgccgcc atgggc
//
