LOCUS       sequence-180889-        4711 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry vector for an inducible N-terminally 3XFLAG-tagged and
            C-terminally AU1-tagged human wtGATA6.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4711)
  AUTHORS   Chitforoushzadeh Z, Ye Z, Sheng Z, LaRue S, Fry RC, Lauffenburger 
            DA, Janes KA
  TITLE     TNF-insulin crosstalk at the transcription factor GATA6 is revealed 
            by a model that links signaling and transcriptomic data tensors.
  JOURNAL   Sci Signal. 2016 Jun 7;9(431):ra59. doi: 10.1126/scisignal.aad3373.
  PUBMED    27273097
REFERENCE   2  (bases 1 to 4711)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2016-06-7"; volume: "9(431):ra59. doi"; pages: " 
            10.1126/scisignal.aad3373"
COMMENT     Sequence Label: pEN_TT 3XFLAG-wtGATA6-3XAU1
FEATURES             Location/Qualifiers
     source          1..4711
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             144..173
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     protein_bind    complement(1052..1151)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1263..1282)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(1341..1360)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     CDS             complement(1934..2467)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(2656..2661,2662..2678,2679..2684))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     rep_origin      3341..3929
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3830..3849
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      4094..4180
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      4272..4299
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     4307..4330
                     /label=pENTR-F
                     /note="pENTR vectors, forward primer"
     protein_bind    4349..4448
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    4488..4506
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4524..4542
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4559..4577
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4595..4613
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4631..4649
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4666..4684
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 cgcgggagcc gggaggctac gcggcggcgg gcagtggggg cgcgggaggc gtgagcggcg
       61 gcggcagtag cctggcggcc atgggcggcc gcgagcccca gtacagctcg ctgtcggccg
      121 cgcggccgct gaacgggacg taccaccacc accaccacca ccaccaccac catccgagcc
      181 cctactcgcc ctacgtgggg gcgccactga cgcctgcctg gcccgccgga cccttcgaga
      241 ccccggtgct gcacagcctg cagagccgcg ccggagcccc gctcccggtg ccccggggtc
      301 ccagtgcaga cctgctggag gacctgtccg agagccgcga gtgcgtgaac tgcggctcca
      361 tccagacgcc gctgtggcgg cgggacggca ccggccacta cctgtgcaac gcctgcgggc
      421 tctacagcaa gatgaacggc ctcagccggc ccctcatcaa gccgcagaag cgcgtgcctt
      481 catcacggcg gcttggattg tcctgtgcca actgtcacac cacaactacc accttatggc
      541 gcagaaacgc cgagggtgaa cccgtgtgca atgcttgtgg actctacatg aaactccatg
      601 gggtgcccag accacttgct atgaaaaaag agggaattca aaccaggaaa cgaaaaccta
      661 agaacataaa taaatcaaag acttgctctg gtaatagcaa taattccatt cccatgactc
      721 caacttccac ctcttctaac tcagatgatt gcagcaaaaa tacttccccc acaacacaac
      781 ctacagcctc aggggcgggt gccccggtga tgactggtgc gggagagagc accaatcccg
      841 agaacagcga gctcaagtat tcgggtcaag atgggctcta cataggcgtc agtctcgcct
      901 cgccggccga agtcacgtcc tccgtgcgac cggattcctg gtgcgccctg gccctggccg
      961 acacctaccg ctacatcgac acctaccgct acatcgacac ctaccgctac atctaacaat
     1021 tggaagacta atgcgttttc gagatatcta gacccagctt tcttgtacaa agttggcatt
     1081 ataagaaagc attgcttatc aatttgttgc aacgaacagg tcactatcag tcaaaataaa
     1141 atcattattt gccatccagc tgcagctctg gcccgtgtct caaaatctct gatgttacat
     1201 tgcacaagat aaaaatatat catcatgaac aataaaactg tctgcttaca taaacagtaa
     1261 tacaaggggt gttatgagcc atattcaacg ggaaacgtcg aggccgcgat taaattccaa
     1321 catggatgct gatttatatg ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc
     1381 gacaatctat cgcttgtatg ggaagcccga tgcgccagag ttgtttctga aacatggcaa
     1441 aggtagcgtt gccaatgatg ttacagatga gatggtcaga ctaaactggc tgacggaatt
     1501 tatgcctctt ccgaccatca agcattttat ccgtactcct gatgatgcat ggttactcac
     1561 cactgcgatc cccggaaaaa cagcattcca ggtattagaa gaatatcctg attcaggtga
     1621 aaatattgtt gatgcgctgg cagtgtccct gcgccggttg cattcgattc ctgtttgtaa
     1681 ttgtcctttt aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa
     1741 cggtttggtt gatgcgagtg attttgatga cgagcgtaat ggctggcctg ttgaacaagt
     1801 ctggaaagaa atgcataaac ttttgccatt ctcaccggat tcagtcgtca ctcatggtga
     1861 tttctcactt gataacctta tttttgacga ggggaaatta ggccgggaag ccgatctcgg
     1921 cttgaacgaa ttgttaggtg gcggtacttg ggtcgatatc aaagtgcatc acttcttccc
     1981 gtatgcccaa ctttgtatag agagccactg cgggatcgtc accgtaatct gcttgcacgt
     2041 agatcacata agcaccaagc gcgttggcct catgcttgag gagattgatg agcgcggtgg
     2101 caatgccctg cctccggtgc tcgccggaga ctgcgagatc atagatatag atctcactac
     2161 gcggctgctc aaacttgggc agaacgtaag ccgcgagagc gccaacaacc gcttcttggt
     2221 cgaaggcagc aagcgcgatg aatgtcttac tacggagcaa gttcccgagg taatcggagt
     2281 ccggctgatg ttgggagtag gtggctacgt ctccgaactc acgaccgaaa agatcaagag
     2341 cagcccgcat ggatttgact tggtcagggc cgagcctaca tgtgcgaatg atgcccatac
     2401 ttgagccacc taactttgtt ttagggcgac tgccctgctg cgtaacatcg ttgctgctgc
     2461 gtaacatcgt tgctgctcca taacatcaaa catcgaccca cggcgtaacg cgcttgctgc
     2521 ttggatgccc gaggcataga ctgtacaaaa aaacagtcat aacaagccat gaaaaccgcc
     2581 actgcgccgt taccaccgct gcgttcggtc aaggttctgg accagttgcg tgagcgcata
     2641 cgctacttgc attacagttt acgaaccgaa caggcttatg tcaactgggt tcgtgccttc
     2701 atccgtttcc acggtgtgcg tcacccggca accttgggca gcagcgaagt cgaggcattt
     2761 ctgtcctggc tggcgaacga gcgcaaggtt tcggtctcca cgcatcgtca ggcattggcg
     2821 gccttgctgt tcttctacgg caaggtgctg tgcacggatc tgccctggct tcaggagatc
     2881 ggaagacctc ggccgtcgcg gcgcttgccg gtggtgctga ccccggatga agtggttcgc
     2941 atcctcggtt ttctggaagg cgagcatcgt ttgttcgccc aggactctag ctatagttct
     3001 agtggttggc tactaatagg ttgtattgat gttggacgag tcggaatcgc agaccgatac
     3061 caggatcttg ccatcctatg gaactgcctc ggtgagtttt ctccttcatt acagaaacgg
     3121 ctttttcaaa aatatggtat tgataatcct gatatgaata aattgcagtt tcatttgatg
     3181 ctcgatgagt ttttctaatc agaattggtt aattggttgt aacactggca gagcattacg
     3241 ctgacttgac gggacggcgc aagctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     3301 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     3361 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     3421 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     3481 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     3541 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     3601 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     3661 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     3721 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     3781 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     3841 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     3901 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     3961 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     4021 ataaccgtat taccgctagc atggatctcg gggacgtcta actactaagc gagagtaggg
     4081 aactgccagg catcaaataa aacgaaaggc tcagtcggaa gactgggcct ttcgttttat
     4141 ctgttgtttg tcggtgaacg ctctcctgag taggacaaat ccgccgggag cggatttgaa
     4201 cgttgtgaag caacggcccg gagggtggcg ggcaggacgc ccgccataaa ctgccaggca
     4261 tcaaactaag cagaaggcca tcctgacgga tggccttttt gcgtttctac aaactcttcc
     4321 tgttagttag ttacttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
     4381 ctgttcgttg caacaaattg ataagcaatg cttttttata atgccaactt tgtacaaaaa
     4441 agcaggcttt aaaggaacca attcagtcga ctggatcccg agtttactcc ctatcagtga
     4501 tagagaacgt atgtcgagtt tactccctat cagtgataga gaacgatgtc gagtttactc
     4561 cctatcagtg atagagaacg tatgtcgagt ttactcccta tcagtgatag agaacgtatg
     4621 tcgagtttac tccctatcag tgatagagaa cgtatgtcga gtttatccct atcagtgata
     4681 gagaacgtat gtcgagttta ctccctatca g
//
