LOCUS       sequence-180892-       14278 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Cas9 with fluorescent Cyan reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14278)
  AUTHORS   Herold
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14278)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: FUCas9Cyan
FEATURES             Location/Qualifiers
     source          1..14278
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     259..278
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     regulatory      470..479
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             479..544
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             551..571
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             596..4696
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             596..4696
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             4697..4744
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             4754..4807
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(4808..4810,4811..4813,4814..5527)
                     /codon_start=1
                     /product="cyan variant of GFP"
                     /label=CFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     AHFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5114..5133)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5461..5482
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    5555..6143
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5608..5628)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6026..6037)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6146..6162)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6147..6163)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6672..6852
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6878..6895)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6884..7108
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7154..7582
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7241..7260)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7451..7472
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7591..7611)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7596..7925
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7776..7911
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7838..7857
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7973..8020
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8039..8413
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8543..8664
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8580..8599)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8634..8653
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8713..8729)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8713..8729)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8726..8748)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8737..8753
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8761..8767,8768..8785,8786..8791))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8806..8827
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8944..8961)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9115..9703)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9195..9214)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9874..10665,10666..10734))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10497..10516
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10735..10839)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10914..10933)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        11105..11484
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        11486..11684
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     11639..11659
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             11702..11882
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    11929..12054
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    12547..12780
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             12965..13009
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    13307..13424
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        join(13489..14278,1..422)
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
ORIGIN
        1 ccgttgggca gtgcacccgt acctttggga gcgcgcgccc tcgtcgtgtc gtgacgtcac
       61 ccgttctgtt ggcttataat gcagggtggg gccacctgcc ggtaggtgtg cggtaggctt
      121 ttctccgtcg caggacgcag ggttcgggcc tagggtaggc tctcctgaat cgacaggcgc
      181 cggacctctg gtgaggggag ggataagtga ggcgtcagtt tctttggtcg gttttatgta
      241 cctatcttct taagtagctg aagctccggt tttgaactat gcgctcgggg ttggcgagtg
      301 tgttttgtga agttttttag gcaccttttg aaatgtaatc atttgggtca atatgtaatt
      361 ttcagtgtta gactagtaaa ttgtccgcta aattctggcc gtttttggct tttttgttag
      421 acgaagcttg ggctgcaggt cgactctaga ggatctgaat tctgacggtg ccaccatgga
      481 ctataaggac cacgacggag actacaagga tcatgatatt gattacaaag acgatgacga
      541 taagatggcc ccaaagaaga agcggaaggt cggtatccac ggagtcccag cagccgacaa
      601 gaagtacagc atcggcctgg acatcggcac caactctgtg ggctgggccg tgatcaccga
      661 cgagtacaag gtgcccagca agaaattcaa ggtgctgggc aacaccgacc ggcacagcat
      721 caagaagaac ctgatcggag ccctgctgtt cgacagcggc gaaacagccg aggccacccg
      781 gctgaagaga accgccagaa gaagatacac cagacggaag aaccggatct gctatctgca
      841 agagatcttc agcaacgaga tggccaaggt ggacgacagc ttcttccaca gactggaaga
      901 gtccttcctg gtggaagagg ataagaagca cgagcggcac cccatcttcg gcaacatcgt
      961 ggacgaggtg gcctaccacg agaagtaccc caccatctac cacctgagaa agaaactggt
     1021 ggacagcacc gacaaggccg acctgcggct gatctatctg gccctggccc acatgatcaa
     1081 gttccggggc cacttcctga tcgagggcga cctgaacccc gacaacagcg acgtggacaa
     1141 gctgttcatc cagctggtgc agacctacaa ccagctgttc gaggaaaacc ccatcaacgc
     1201 cagcggcgtg gacgccaagg ccatcctgtc tgccagactg agcaagagca gacggctgga
     1261 aaatctgatc gcccagctgc ccggcgagaa gaagaatggc ctgttcggaa acctgattgc
     1321 cctgagcctg ggcctgaccc ccaacttcaa gagcaacttc gacctggccg aggatgccaa
     1381 actgcagctg agcaaggaca cctacgacga cgacctggac aacctgctgg cccagatcgg
     1441 cgaccagtac gccgacctgt ttctggccgc caagaacctg tccgacgcca tcctgctgag
     1501 cgacatcctg agagtgaaca ccgagatcac caaggccccc ctgagcgcct ctatgatcaa
     1561 gagatacgac gagcaccacc aggacctgac cctgctgaaa gctctcgtgc ggcagcagct
     1621 gcctgagaag tacaaagaga ttttcttcga ccagagcaag aacggctacg ccggctacat
     1681 tgacggcgga gccagccagg aagagttcta caagttcatc aagcccatcc tggaaaagat
     1741 ggacggcacc gaggaactgc tcgtgaagct gaacagagag gacctgctgc ggaagcagcg
     1801 gaccttcgac aacggcagca tcccccacca gatccacctg ggagagctgc acgccattct
     1861 gcggcggcag gaagattttt acccattcct gaaggacaac cgggaaaaga tcgagaagat
     1921 cctgaccttc cgcatcccct actacgtggg ccctctggcc aggggaaaca gcagattcgc
     1981 ctggatgacc agaaagagcg aggaaaccat caccccctgg aacttcgagg aagtggtgga
     2041 caagggcgct tccgcccaga gcttcatcga gcggatgacc aacttcgata agaacctgcc
     2101 caacgagaag gtgctgccca agcacagcct gctgtacgag tacttcaccg tgtataacga
     2161 gctgaccaaa gtgaaatacg tgaccgaggg aatgagaaag cccgccttcc tgagcggcga
     2221 gcagaaaaag gccatcgtgg acctgctgtt caagaccaac cggaaagtga ccgtgaagca
     2281 gctgaaagag gactacttca agaaaatcga gtgcttcgac tccgtggaaa tctccggcgt
     2341 ggaagatcgg ttcaacgcct ccctgggcac ataccacgat ctgctgaaaa ttatcaagga
     2401 caaggacttc ctggacaatg aggaaaacga ggacattctg gaagatatcg tgctgaccct
     2461 gacactgttt gaggacagag agatgatcga ggaacggctg aaaacctatg cccacctgtt
     2521 cgacgacaaa gtgatgaagc agctgaagcg gcggagatac accggctggg gcaggctgag
     2581 ccggaagctg atcaacggca tccgggacaa gcagtccggc aagacaatcc tggatttcct
     2641 gaagtccgac ggcttcgcca acagaaactt catgcagctg atccacgacg acagcctgac
     2701 ctttaaagag gacatccaga aagcccaggt gtccggccag ggcgatagcc tgcacgagca
     2761 cattgccaat ctggccggca gccccgccat taagaagggc atcctgcaga cagtgaaggt
     2821 ggtggacgag ctcgtgaaag tgatgggccg gcacaagccc gagaacatcg tgatcgaaat
     2881 ggccagagag aaccagacca cccagaaggg acagaagaac agccgcgaga gaatgaagcg
     2941 gatcgaagag ggcatcaaag agctgggcag ccagatcctg aaagaacacc ccgtggaaaa
     3001 cacccagctg cagaacgaga agctgtacct gtactacctg cagaatgggc gggatatgta
     3061 cgtggaccag gaactggaca tcaaccggct gtccgactac gatgtggacc atatcgtgcc
     3121 tcagagcttt ctgaaggacg actccatcga caacaaggtg ctgaccagaa gcgacaagaa
     3181 ccggggcaag agcgacaacg tgccctccga agaggtcgtg aagaagatga agaactactg
     3241 gcggcagctg ctgaacgcca agctgattac ccagagaaag ttcgacaatc tgaccaaggc
     3301 cgagagaggc ggcctgagcg aactggataa ggccggcttc atcaagagac agctggtgga
     3361 aacccggcag atcacaaagc acgtggcaca gatcctggac tcccggatga acactaagta
     3421 cgacgagaat gacaagctga tccgggaagt gaaagtgatc accctgaagt ccaagctggt
     3481 gtccgatttc cggaaggatt tccagtttta caaagtgcgc gagatcaaca actaccacca
     3541 cgcccacgac gcctacctga acgccgtcgt gggaaccgcc ctgatcaaaa agtaccctaa
     3601 gctggaaagc gagttcgtgt acggcgacta caaggtgtac gacgtgcgga agatgatcgc
     3661 caagagcgag caggaaatcg gcaaggctac cgccaagtac ttcttctaca gcaacatcat
     3721 gaactttttc aagaccgaga ttaccctggc caacggcgag atccggaagc ggcctctgat
     3781 cgagacaaac ggcgaaaccg gggagatcgt gtgggataag ggccgggatt ttgccaccgt
     3841 gcggaaagtg ctgagcatgc cccaagtgaa tatcgtgaaa aagaccgagg tgcagacagg
     3901 cggcttcagc aaagagtcta tcctgcccaa gaggaacagc gataagctga tcgccagaaa
     3961 gaaggactgg gaccctaaga agtacggcgg cttcgacagc cccaccgtgg cctattctgt
     4021 gctggtggtg gccaaagtgg aaaagggcaa gtccaagaaa ctgaagagtg tgaaagagct
     4081 gctggggatc accatcatgg aaagaagcag cttcgagaag aatcccatcg actttctgga
     4141 agccaagggc tacaaagaag tgaaaaagga cctgatcatc aagctgccta agtactccct
     4201 gttcgagctg gaaaacggcc ggaagagaat gctggcctct gccggcgaac tgcagaaggg
     4261 aaacgaactg gccctgccct ccaaatatgt gaacttcctg tacctggcca gccactatga
     4321 gaagctgaag ggctcccccg aggataatga gcagaaacag ctgtttgtgg aacagcacaa
     4381 gcactacctg gacgagatca tcgagcagat cagcgagttc tccaagagag tgatcctggc
     4441 cgacgctaat ctggacaaag tgctgtccgc ctacaacaag caccgggata agcccatcag
     4501 agagcaggcc gagaatatca tccacctgtt taccctgacc aatctgggag cccctgccgc
     4561 cttcaagtac tttgacacca ccatcgaccg gaagaggtac accagcacca aagaggtgct
     4621 ggacgccacc ctgatccacc agagcatcac cggcctgtac gagacacgga tcgacctgtc
     4681 tcagctggga ggcgacaaaa ggccggcggc cacgaaaaag gccggccagg caaaaaagaa
     4741 aaagggatcc ggtgagggca gaggaagtct gctaacatgc ggtgacgtcg aggagaatcc
     4801 tggcccaatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
     4861 gctggacggc gacgtaaacg gccacaggtt cagcgtgtcc ggcgagggcg agggcgatgc
     4921 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg
     4981 gcccaccctc gtgaccaccc tgacctgggg cgtgcagtgc ttcagccgct accccgacca
     5041 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgtac
     5101 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
     5161 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
     5221 ggggcacaag ctggagtaca actacatcag ccacaacgtc tatatcaccg ccgacaagca
     5281 gaagaacggc atcaaggccc acttcaagat ccgccacaac atcgaggacg gcagcgtgca
     5341 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
     5401 caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca
     5461 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta
     5521 caagtgatga caattcgata tcaagcttat cgataatcaa cctctggatt acaaaatttg
     5581 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
     5641 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
     5701 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
     5761 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
     5821 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
     5881 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
     5941 gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg
     6001 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg
     6061 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat
     6121 ctccctttgg gccgcctccc cgcatcgata ccgtcgacct cgatcgagac ctagaaaaac
     6181 atggagcaat cacaagtagc aatacagcag ctaccaatgc tgattgtgcc tggctagaag
     6241 cacaagagga ggaggaggtg ggttttccag tcacacctca ggtaccttta agaccaatga
     6301 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc
     6361 taattcactc ccaacgaaga caagatatcc ttgatctgtg gatctaccac acacaaggct
     6421 acttccctga ttggcagaac tacacaccag ggccagggat cagatatcca ctgacctttg
     6481 gatggtgcta caagctagta ccagttgagc aagagaaggt agaagaagcc aatgaaggag
     6541 agaacacccg cttgttacac cctgtgagcc tgcatgggat ggatgacccg gagagagaag
     6601 tattagagtg gaggtttgac agccgcctag catttcatca catggcccga gagctgcatc
     6661 cggactgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     6721 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     6781 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     6841 aaatctctag cagggcccgt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc
     6901 cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc
     6961 actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct
     7021 attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg
     7081 catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct
     7141 agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     7201 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     7261 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     7321 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     7381 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     7441 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
     7501 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
     7561 taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt
     7621 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     7681 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     7741 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
     7801 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     7861 cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
     7921 gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcagcac gtgttgacaa
     7981 ttaatcatcg gcatagtata tcggcatagt ataatacgac aaggtgagga actaaaccat
     8041 ggccaagttg accagtgccg ttccggtgct caccgcgcgc gacgtcgccg gagcggtcga
     8101 gttctggacc gaccggctcg ggttctcccg ggacttcgtg gaggacgact tcgccggtgt
     8161 ggtccgggac gacgtgaccc tgttcatcag cgcggtccag gaccaggtgg tgccggacaa
     8221 caccctggcc tgggtgtggg tgcgcggcct ggacgagctg tacgccgagt ggtcggaggt
     8281 cgtgtccacg aacttccggg acgcctccgg gccggccatg accgagatcg gcgagcagcc
     8341 gtgggggcgg gagttcgccc tgcgcgaccc ggccggcaac tgcgtgcact tcgtggccga
     8401 ggagcaggac tgacacgtgc tacgagattt cgattccacc gccgccttct atgaaaggtt
     8461 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat
     8521 gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag
     8581 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     8641 gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt
     8701 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     8761 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     8821 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     8881 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     8941 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     9001 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     9061 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     9121 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     9181 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     9241 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     9301 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     9361 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     9421 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     9481 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     9541 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     9601 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     9661 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     9721 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     9781 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     9841 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     9901 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     9961 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
    10021 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
    10081 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
    10141 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
    10201 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
    10261 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
    10321 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
    10381 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
    10441 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
    10501 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
    10561 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
    10621 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
    10681 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
    10741 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
    10801 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
    10861 acctgacgtc gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc
    10921 tgctctgatg ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct
    10981 gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg
    11041 aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg
    11101 cgttgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat
    11161 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
    11221 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
    11281 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
    11341 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
    11401 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
    11461 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
    11521 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
    11581 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
    11641 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagcgcgtt ttgcctgtac
    11701 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
    11761 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
    11821 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
    11881 cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc
    11941 aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg
    12001 ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt
    12061 aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa
    12121 aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat
    12181 cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc
    12241 cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt
    12301 gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag
    12361 caaaacaaaa gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg
    12421 agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc
    12481 attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt
    12541 gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc
    12601 gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa
    12661 caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat
    12721 caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct
    12781 ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag
    12841 ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag
    12901 agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca
    12961 agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt
    13021 taacataaca aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt
    13081 aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc
    13141 accattatcg tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat
    13201 agaagaagaa ggtggagaga gagacagaga cagatccatt cgattagtga acggatcggc
    13261 actgcgtgcg ccaattctgc agacaaatgg cagtattcat ccacaatttt aaaagaaaag
    13321 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
    13381 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
    13441 acagcagaga tccagtttgg ttaattaacc cgtgtcggct ccagatctgg cctccgcgcc
    13501 gggttttggc gcctcccgcg ggcgcccccc tcctcacggc gagcgctgcc acgtcagacg
    13561 aagggcgcag cgagcgtcct gatccttccg cccggacgct caggacagcg gcccgctgct
    13621 cataagactc ggccttagaa ccccagtatc agcagaagga cattttagga cgggacttgg
    13681 gtgactctag ggcactggtt ttctttccag agagcggaac aggcgaggaa aagtagtccc
    13741 ttctcggcga ttctgcggag ggatctccgt ggggcggtga acgccgatga ttatataagg
    13801 acgcgccggg tgtggcacag ctagttccgt cgcagccggg atttgggtcg cggttcttgt
    13861 ttgtggatcg ctgtgatcgt cacttggtga gtagcgggct gctgggctgg ccggggcttt
    13921 cgtggccgcc gggccgctcg gtgggacgga agcgtgtgga gagaccgcca agggctgtag
    13981 tctgggtccg cgagcaaggt tgccctgaac tgggggttgg ggggagcgca gcaaaatggc
    14041 ggctgttccc gagtcttgaa tggaagacgc ttgtgaggcg ggctgtgagg tcgttgaaac
    14101 aaggtggggg gcatggtggg cggcaagaac ccaaggtctt gaggccttcg ctaatgcggg
    14161 aaagctctta ttcgggtgag atgggctggg gcaccatctg gggaccctga cgtgaagttt
    14221 gtcactgact ggagaactcg gtttgtcgtc tgttgcgggg gcggcagtta tggcggtg
//
