LOCUS       sequence-180893-        1071 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Huntingtin Chromobody 1: ""Happ1"" intrabody recognizing amino acids
            41–81 of huntingtin, fused to eYFP..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1071)
  AUTHORS   Maiuri T, Mocle AJ, Hung CL, Xia J, van Roon-Mom WM, Truant R
  TITLE     Huntingtin is a scaffolding protein in the ATM oxidative DNA damage 
            response complex.
  JOURNAL   Hum Mol Genet. 2016 Dec 25. pii: ddw395. doi: 10.1093/hmg/ddw395.
  PUBMED    28017939
REFERENCE   2  (bases 1 to 1071)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Hum Mol 
            Genet. 2016 Dec 25. pii: ddw395. doi: 10.1093/hmg/ddw395."
COMMENT     Sequence Label: HCB1
FEATURES             Location/Qualifiers
     source          1..1071
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(190..702,703..705,706..708))
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIE"
     primer_bind     383..402
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     642..663
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 gctcgtcntg ccgagnntga tcccggcggc ggtcacgaac tccagcaggn ncatgtgatc
       61 gcgcttctcg ttggggtctt tgctcagggc ggactggtag ctcaggtagt ggttgtcggg
      121 cagcagcacg gggccgtcgc cgatgggggt gttctgctgg tagtggtcgg cgagctgcac
      181 gctgccgtcc tcgatgttgt ggcggatctt gaagttcacc ttgatgccgt tcttctgctt
      241 gtcggccatg atatagacgt tgtggctgtt gtagttgtac tccagcttgt gccccaggat
      301 gttgccgtcc tccttgaagt cgatgccctt cagctcgatg cggttcacca gggtgtcgcc
      361 ctcgaacttc acctcggcgc gggtcttgta gttgccgtcg tccttgaaga agatggtgcg
      421 ctcctggacg tagccttcgg gcatggcgga cttgaagaag tcgtgctgct tcatgtggtc
      481 ggggtagcgg gcgaagcact gcaggccgta gccgaaggtg gtcacgaggg tgggccaggg
      541 cacgggcagc ttgccggtgg tgcagatgaa cttcagggtc agcttgccgt aggtggcatc
      601 gccctcgccc tcgccggaca cgctgaactt gtggccgttt acgtcgccgt ccagctcgac
      661 caggatgggc accaccccgg tgaacagctc ctcgcccttg ctcaccatgg atcccgggcc
      721 cgcggtaccg tcgactccac ctccaccgtt ggtccctccg ccgaatacca gagcaacaca
      781 caggctgtca tcccatgctg cacagtaata atcagcctca tcctcgggcc ggagcccact
      841 gatggccagg gaggctgang tgccagactt ggagccngan aatcggtcag gganccctga
      901 gggccgctga ttattcctat agatgaggag tttgggggcc gttcctggga gctgctggta
      961 ccagtataca taattacttc cgatgttgga gctgcttcca gaacaagana nngtgaccct
     1021 ctgcccgggg tcccanncgc tgagggtggc tgagtcagca cagnctgcat t
//
