LOCUS       sequence-180894-        1062 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Nuclear Huntingtin Chromobody 1: ""Happ1"" intrabody recognizing 
            amino acids 41–81 of huntingtin, fused to eYFP and nuclear 
            localization signal..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Maiuri T, Mocle AJ, Hung CL, Xia J, van Roon-Mom WM, Truant R
  TITLE     Huntingtin is a scaffolding protein in the ATM oxidative DNA damage 
            response complex.
  JOURNAL   Hum Mol Genet. 2016 Dec 25. pii: ddw395. doi: 10.1093/hmg/ddw395.
  PUBMED    28017939
REFERENCE   2  (bases 1 to 1062)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Hum Mol 
            Genet. 2016 Dec 25. pii: ddw395. doi: 10.1093/hmg/ddw395."
COMMENT     Sequence Label: nucHCB1
FEATURES             Location/Qualifiers
     source          1..1062
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(397..399,400..402,403..915)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELXGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIE"
     primer_bind     complement(442..463)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(703..722)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 gctagcgcta ccggtcgcnc catggtgtcc ggaatgcagt ctgtgctgac tcagccnccc
       61 tcagcgtctg ggacccccgg gcaganggtc accatctctt gttctggaag cagctccaac
      121 atcggaagta attatgtata ctggtaccag cagctcccag gaacggcccc caaactcctc
      181 atctatagga ataatcagcg gccctcaggg gtccctgacc gattctctgg ctccaagtct
      241 ggcacctcag cctccctggc catcagtggg ctccggcccg aggatgaggc tgattattac
      301 tgtgcagcat gggatgacag cctgtgtgtt gctctggtat tcggcggagg gaccaacggt
      361 ggaggtggag tcgacggtac cgcgggcccg ggatccatgg tgagcaaggg cgaggagctg
      421 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc
      481 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc
      541 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccacctt cggctacggc
      601 ctgcagtgct tcgcccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc
      661 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag
      721 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaanggc
      781 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc
      841 cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc
      901 cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc
      961 atcggcnacg gccccgtgct gctgcccgac aaccactacc tgannnccag tccgccctga
     1021 ncaaagaccc caacgagaag cgcgatcaca tggtcctgct gg
//
