LOCUS       sequence-180956-        6946 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6946)
  AUTHORS   Takahashi R, Deveraux Q, Tamm I, Welsh K, Assa-Munt N, Salvesen GS, 
            Reed JC
  TITLE     A single BIR domain of XIAP sufficient for inhibiting caspases.
  JOURNAL   J Biol Chem. 1998 Apr 3. 273(14):7787-90.
  PUBMED    9525868
REFERENCE   2  (bases 1 to 6946)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 1998 Apr 3. 273(14):7787-90."
COMMENT     Sequence Label: pcDNA3-Xiap-Myc
FEATURES             Location/Qualifiers
     source          1..6946
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      156..291
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     218..237
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             372..1166
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(426..445)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1036..1055
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    1340..1461
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1377..1396)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1431..1450
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1510..1526)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1510..1526)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1523..1545)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1534..1550
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1558..1564,1565..1582,1583..1588))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1603..1624
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1741..1758)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1912..2500)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1992..2011)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2671..3462,3463..3531))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3294..3313
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3532..3636)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3711..3730)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3902..4281
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4282..4485
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4436..4456
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4530..4549
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4530..4548
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4576..4605
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     promoter        complement(6166..6184)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(6167..6184)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(6204..6221)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6210..6434
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6480..6908
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6567..6586)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6777..6798
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6917..6937)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        join(6922..6946,1..305)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
ORIGIN
        1 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
       61 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
      121 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
      181 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
      241 cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
      301 gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcaagag acaggatgag
      361 gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg
      421 agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt
      481 tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc
      541 tgaatgaact gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt
      601 gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag
      661 tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg
      721 ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag
      781 cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg
      841 atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc
      901 gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca
      961 tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc
     1021 gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg
     1081 ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct
     1141 atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc
     1201 gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg
     1261 cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct
     1321 ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa
     1381 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     1441 caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc
     1501 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     1561 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     1621 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     1681 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     1741 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     1801 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     1861 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     1921 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     1981 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     2041 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     2101 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     2161 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     2221 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     2281 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     2341 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     2401 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     2461 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     2521 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     2581 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     2641 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     2701 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     2761 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     2821 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     2881 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     2941 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     3001 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     3061 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     3121 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     3181 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     3241 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     3301 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     3361 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     3421 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     3481 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     3541 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     3601 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     3661 tgacgtcgac ggatcgggag atctcccgat cccctatggt gcactctcag tacaatctgc
     3721 tctgatgccg catagttaag ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag
     3781 tagtgcgcga gcaaaattta agctacaaca aggcaaggct tgaccgacaa ttgcatgaag
     3841 aatctgctta gggttaggcg ttttgcgctg cttcgcgatg tacgggccag atatacgcgt
     3901 tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc
     3961 ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc
     4021 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg
     4081 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
     4141 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc
     4201 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta
     4261 ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag
     4321 cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt
     4381 tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa
     4441 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctctctg gctaactaga
     4501 gaacccactg cttactggct tatcgaaatt aatacgactc actataggga gacccaagct
     4561 tcgatcgtcg acatggaaca gaaactcatc tctgaagagg atctgtctag aggatccgaa
     4621 ttcatgactt ttaacagttt tgaaggatct aaaacttgtg tacctgcaga catcaataag
     4681 gaagaagaat ttgtagaaga gtttaataga ttaaaaactt ttgctaattt tccaagtggt
     4741 agtcctgttt cagcatcaac actggcacga gcagggtttc tttatactgg tgaaggagat
     4801 accgtgcggt gctttagttg tcatgcagct gtagataggt ggcaatatgg agactcagca
     4861 gttggaagac acaggaaagt atccccaaat tgcagattta tcaacggctt ttatcttgaa
     4921 aatagtgcca cgcagtctac aaattctggt atccagaatg gtcagtacaa agttgaaaac
     4981 tatctgggaa gcagagatca ttttgcctta gacaggccat ctgagacaca tgcagactat
     5041 cttttgagaa ctgggcaggt tgtagatata tcagacacca tatacccgag gaaccctgcc
     5101 atgtatagtg aagaagctag attaaagtcc tttcagaact ggccagacta tgctcaccta
     5161 accccaagag agttagcaag tgctggactc tactacacag gtattggtga ccaagtgcag
     5221 tgcttttgtt gtggtggaaa actgaaaaat tgggaacctt gtgatcgtgc ctggtcagaa
     5281 cacaggcgac actttcctaa ttgcttcttt gttttgggcc ggaatcttaa tattcgaagt
     5341 gaatctgatg ctgtgagttc tgataggaat ttcccaaatt caacaaatct tccaagaaat
     5401 ccatccatgg cagattatga agcacggatc tttacttttg ggacatggat atactcagtt
     5461 aacaaggagc agcttgcaag agctggattt tatgctttag gtgaaggtga taaagtaaag
     5521 tgctttcact gtggaggagg gctaactgat tggaagccca gtgaagaccc ttgggaacaa
     5581 catgctaaat ggtatccagg gtgcaaatat ctgttagaac agaagggaca agaatatata
     5641 aacaatattc atttaactca ttcacttgag gagtgtctgg taagaactac tgagaaaaca
     5701 ccatcactaa ctagaagaat tgatgatacc atcttccaaa atcctatggt acaagaagct
     5761 atacgaatgg ggttcagttt caaggacatt aagaaaataa tggaggaaaa aattcagata
     5821 tctgggagca actataaatc acttgaggtt ctggttgcag atctagtgaa tgctcagaaa
     5881 gacagtatgc aagatgagtc aagtcagact tcattacaga aagagattag tactgaagag
     5941 cagctaaggc gcctgcaaga ggagaagctt tgcaaaatct gtatggatag aaatattgct
     6001 atcgtttttg ttccttgtgg acatctagtc acttgtaaac aatgtgctga agcagttgac
     6061 aagtgtccca tgtgctacac agtcattact ttcaagcaaa aaatttttat gtcttaatct
     6121 aactctatag taggcatgct cgagcatgca tctagagggc cctattctat agtgtcacct
     6181 aaatgctaga gctcgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt
     6241 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta
     6301 ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg
     6361 ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc
     6421 ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca
     6481 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     6541 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     6601 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     6661 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
     6721 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     6781 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
     6841 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     6901 cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagt
//
