LOCUS       sequence-181034-        8088 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  constitutive expression of DDX5 D248N in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8088)
  AUTHORS   Huang W, Thomas B, Flynn RA, Gavzy SJ, Wu L, Kim SV, Hall JA, 
            Miraldi ER, Ng CP, Rigo F, Meadows S, Montoya NR, Herrera NG, 
            Domingos AI, Rastinejad F, Myers RM, Fuller-Pace FV, Bonneau R, 
            Chang HY, Acuto O, Littman DR
  TITLE     DDX5 and its associated lncRNA Rmrp modulate TH17 cell effector 
            functions.
  JOURNAL   Nature. 2015 Dec 24;528(7583):517-22. doi: 10.1038/nature16193. Epub
            2015 Dec 16.
  PUBMED    26675721
REFERENCE   2  (bases 1 to 8088)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature16193"; journalName: "Nature"; date: "2015-12-24- 
            24"; volume: "528"; issue: "7583"; pages: "517-22"
COMMENT     Sequence Label: pMThy1.1_hDDX5_FL_D248N
FEATURES             Location/Qualifiers
     source          1..8088
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..517
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
     misc_feature    581..922
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             989..1405
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1335..1357
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1373..1389
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    3323..3889
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    3339..3891
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3483..3500)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3710..3729
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3939..4427
                     /codon_start=1
                     /product="mouse GPI-anchored cell surface glycoprotein of 
                     the immunoglobulin superfamily"
                     /label=Thy1.1
                     /note="This allele of Thy1 is also known as CD90.1."
                     /translation="MNPAISVALLLSVLQVSRGQKVTSLTACLVNQNLRLDCRHENNTK
                     DNSIQHEFSLTREKRKHVLSGTLGIPEHTYRSRVTLSNQPYIKVLTLANFTTKDEGDYF
                     CELRVSGANPMSSNKSISVYRDKLVKCGGISLLVQNTSWMLLLLLSLSLLQALDFISL"
     LTR             4483..4997
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     protein_bind    5159..5175
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5183..5189,5190..5207,5208..5213))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5228..5249
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5366..5383)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5537..6125)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5617..5636)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6296..7087,7088..7156))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6919..6938
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7157..7261)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7329..7347
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7385..7407)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7507..7526
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7720..7742
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7870..7889
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 aatgaaagac cccacctgta ggtttggcaa gctagcttaa gtaacgccat tttgcaaggc
       61 atggaaaata cataactgag aatagagaag ttcagatcaa ggttaggaac agagagacag
      121 cagaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa
      181 gaacagatgg tccccagatg cggtcccgcc ctcagcagtt tctagagaac catcagatgt
      241 ttccagggtg ccccaaggac ctgaaatgac cctgtgcctt atttgaacta accaatcagt
      301 tcgcttctcg cttctgttcg cgcgcttctg ctccccgagc tcaataaaag agcccacaac
      361 ccctcactcg gcgcgccagt cctccgatag actgcgtcgc ccgggtaccc gtattcccaa
      421 taaagcctct tgctgtttgc atccgaatcg tggactcgct gatccttggg agggtctcct
      481 cagattgatt gactgcccac ctcgggggtc tttcatttgg aggttccacc gagatttgga
      541 gacccctgcc cagggaccac cgaccccccc gccgggaggt aagctggcca gcggtcgttt
      601 cgtgtctgtc tctgtctttg tgcgtgtttg tgccggcatc taatgtttgc gcctgcgtct
      661 gtactagtta gctaactagc tctgtatctg gcggacccgt ggtggaactg acgagttctg
      721 aacacccggc cgcaaccctg ggagacgtcc cagggacttt gggggccgtt tttgtggccc
      781 gacctgagga agggagtcga tgtggaatcc gaccccgtca ggatatgtgg ttctggtagg
      841 agacgagaac ctaaaacagt tcccgcctcc gtctgaattt ttgctttcgg tttggaaccg
      901 aagccgcgcg tcttgtctgc tgcagcgctg cagcatcgtt ctgtgttgtc tctgtctgac
      961 tgtgtttctg tatttgtctg aaaattaggg ccagactgtt accactccct taagtttgac
     1021 cttaggtcac tggaaagatg tcgagcggat cgctcacaac cagtcggtag atgtcaagaa
     1081 gagacgttgg gttaccttct gctctgcaga atggccaacc tttaacgtcg gatggccgcg
     1141 agacggcacc tttaaccgag acctcatcac ccaggttaag atcaaggtct tttcacctgg
     1201 cccgcatgga cacccagacc aggtccccta catcgtgacc tgggaagcct tggcttttga
     1261 cccccctccc tgggtcaagc cctttgtaca ccctaagcct ccgcctcctc ttcctccatc
     1321 cgccccgtct ctcccccttg aacctcctcg ttcgaccccg cctcgatcct ccctttatcc
     1381 agccctcact ccttctctag gcgccggaat tagatctctc gagatcgatg cggccgcgcc
     1441 gccaccatgt cgggttattc gagtgaccga gaccgcggcc gggaccgagg gtttggtgca
     1501 cctcgatttg gaggaagtag ggcagggccc ttatctggaa agaagtttgg aaaccctggg
     1561 gagaaattag ttaaaaagaa gtggaatctt gatgagctgc ctaaatttga gaagaatttt
     1621 tatcaagagc accctgattt ggctaggcgc acagcacaag aggtggaaac atacagaaga
     1681 agcaaggaaa ttacagttag aggtcacaac tgcccgaagc cagttctaaa tttttatgaa
     1741 gccaatttcc ctgcaaatgt catggatgtt attgcaagac agaatttcac tgaacccact
     1801 gctattcaag ctcagggatg gccagttgct ctaagtggat tggatatggt tggagtggca
     1861 cagactggat ctgggaaaac attgtcttat ttgcttcctg ccattgtcca catcaatcat
     1921 cagccattcc tagagagagg cgatgggcct atttgtttgg tgctggcacc aactcgggaa
     1981 ctggcccaac aggtgcagca agtagctgct gaatattgta gagcatgtcg cttgaagtct
     2041 acttgtatct acggtggtgc tcctaaggga ccacaaatac gtgatttgga gagaggtgtg
     2101 gaaatctgta ttgcaacacc tggaagactg attgactttt tagagtgtgg aaaaaccaat
     2161 ctgagaagaa caacctacct tgtccttaat gaagcagata gaatgcttga tatgggcttt
     2221 gaaccccaaa taaggaagat tgtggatcaa ataagacctg ataggcaaac tctaatgtgg
     2281 agtgcgactt ggccaaaaga agtaagacag cttgctgaag atttcctgaa agactatatt
     2341 catataaaca ttggtgcact tgaactgagt gcaaaccaca acattcttca gattgtggat
     2401 gtgtgtcatg acgtagaaaa ggatgaaaaa cttattcgtc taatggaaga gatcatgagt
     2461 gagaaggaga ataaaaccat tgtttttgtg gaaaccaaaa gaagatgtga tgagcttacc
     2521 agaaaaatga ggagagatgg gtggcctgcc atgggtatcc atggtgacaa gagtcaacaa
     2581 gagcgtgact gggttctaaa tgaattcaaa catggaaaag ctcctattct gattgctaca
     2641 gatgtggcct ccagagggct agatgtggaa gatgtgaaat ttgtcatcaa ttatgactac
     2701 cctaactcct cagaggatta tattcatcga attggaagaa ctgctcgcag taccaaaaca
     2761 ggcacagcat acactttctt tacacctaat aacataaagc aagtgagcga ccttatctct
     2821 gtgcttcgtg aagctaatca agcaattaat cccaagttgc ttcagttggt cgaagacaga
     2881 ggttcaggtc gttccagggg tagaggaggc atgaaggatg accgtcggga cagatactct
     2941 gcgggcaaaa ggggtggatt taataccttt agagacaggg aaaattatga cagaggttac
     3001 tctagcctgc ttaaaagaga ttttggggca aaaactcaga atggtgttta cagtgctgca
     3061 aattacacca atgggagctt tggaagtaat tttgtgtctg ctggtataca gaccagtttt
     3121 aggactggta atccaacagg gacttaccag aatggttatg atagcactca gcaatacgga
     3181 agtaatgttc caaatatgca caatggtatg aaccaacagg catatgcata tcctgctact
     3241 gcagctgcac ctatgattgg ttatccaatg ccaacaggat attcccaata agtcgacggt
     3301 atcgataagc ttgatatcga attccgcccc cccccctaac gttactggcc gaagccgctt
     3361 ggaataaggc cggtgtgcgt ttgtctatat gttattttcc accatattgc cgtcttttgg
     3421 caatgtgagg gcccggaaac ctggccctgt cttcttgacg agcattccta ggggtctttc
     3481 ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga
     3541 agcttcttga agacaaacaa cgtctgtagc gaccctttgc aggcagcgga accccccacc
     3601 tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa gatacacctg caaaggcggc
     3661 acaaccccag tgccacgttg tgagttggat agttgtggaa agagtcaaat ggctctcctc
     3721 aagcgtattc aacaaggggc tgaaggatgc ccagaaggta ccccattgta tgggatctga
     3781 tctggggcct cggtgcacat gctttacatg tgtttagtcg aggttaaaaa aacgtctagg
     3841 ccccccgaac cacggggacg tggttttcct ttgaaaaaca cgatgataat atggccacaa
     3901 ccatgggtct tttctgcagt caccgtcctc gaggcaccat gaacccagcc atcagcgtcg
     3961 ctctcctgct ctcagtcttg caggtgtccc gagggcagaa ggtgaccagc ctgacagcct
     4021 gcctggtgaa ccaaaacctt cgcctggact gccgccatga gaataacacc aaggataact
     4081 ccatccagca tgagttcagc ctgacccgag agaagaggaa gcacgtgctc tcaggcaccc
     4141 ttgggatacc cgagcacacg taccgctccc gcgtcaccct ctccaaccag ccctatatca
     4201 aggtccttac cctagccaac ttcaccacca aggatgaggg cgactacttt tgtgagcttc
     4261 gcgtctcggg cgcgaatccc atgagctcca ataaaagtat cagtgtgtat agagacaagc
     4321 tggtcaagtg tggcggcata agcctgctgg ttcagaacac atcctggatg ctgctgctgc
     4381 tgctttccct ctccctcctc caagccctgg acttcatttc tctgtgacgc gtagaattcg
     4441 ataaaataaa agattttatt tagtctccag aaaaaggggg gaatgaaaga ccccacctgt
     4501 aggtttggca agctagctta agtaacgcca ttttgcaagg catggaaaat acataactga
     4561 gaatagagaa gttcagatca aggttaggaa cagagagaca gcagaatatg ggccaaacag
     4621 gatatctgtg gtaagcagtt cctgccccgg ctcagggcca agaacagatg gtccccagat
     4681 gcggtcccgc cctcagcagt ttctagagaa ccatcagatg tttccagggt gccccaagga
     4741 cctgaaatga ccctgtgcct tatttgaact aaccaatcag ttcgcttctc gcttctgttc
     4801 gcgcgcttct gctccccgag ctcaataaaa gagcccacaa cccctcactc ggcgcgccag
     4861 tcctccgata gactgcgtcg cccgggtacc cgtgtatcca ataaaccctc ttgcagttgc
     4921 atccgacttg tggtctcgct gttccttggg agggtctcct ctgagtgatt gactacccgt
     4981 cagcgggggt ctttcatggg taacagtttc ttgaagttgg agaacaacat tctgagggta
     5041 ggagtcgaat attaagtaat cctgactcaa ttagccactg ttttgaatcc acatactcca
     5101 atactcctga aatagttcat tatggacagc gcagaaagag ctggggagaa ttgtgaaatt
     5161 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     5221 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     5281 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     5341 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     5401 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     5461 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     5521 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     5581 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     5641 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     5701 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     5761 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     5821 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     5881 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     5941 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     6001 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     6061 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     6121 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     6181 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     6241 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     6301 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     6361 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     6421 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     6481 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     6541 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     6601 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     6661 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     6721 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     6781 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     6841 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     6901 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     6961 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     7021 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     7081 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     7141 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     7201 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     7261 gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa
     7321 cctataaaaa taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg
     7381 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg
     7441 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta
     7501 actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc
     7561 acagatgcgt aaggagaaaa taccgcatca ggcgccattc gccattcagg ctgcgcaact
     7621 gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat
     7681 gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa
     7741 cgacggcgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg
     7801 gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc
     7861 ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat
     7921 gccggccacg atgcgtccgg cgtagaggcg attagtccaa tttgttaaag acaggatatc
     7981 agtggtccag gctctagttt tgactcaaca atatcaccag ctgaagccta tagagtacga
     8041 gccatagata aaataaaaga ttttatttag tctccagaaa aagggggg
//
