LOCUS       sequence-181037-       11677 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Yeast expression of S. cerevisiae Rpb1 with an internal PreScission 
            protease site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 11677)
  AUTHORS   Sdano MA, Fulcher JM, Palani S, Chandrasekharan MB, Parnell TJ, 
            Whitby FG, Formosa T, Hill CP
  TITLE     A novel SH2 recognition mechanism recruits Spt6 to the doubly 
            phosphorylated RNA polymerase II linker at sites of transcription.
  JOURNAL   Elife. 2017 Aug 16;6. pii: 28723. doi: 10.7554/eLife.28723.
  PUBMED    28826505
REFERENCE   2  (bases 1 to 11677)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2017-08-16"; volume: "6. pii: 28723. doi"; pages: " 
            10.7554/eLife.28723"
COMMENT     Sequence Label: CPH3400  (YCp LEU2 Rpb1 A1529_G1534delInsLEVLF...
FEATURES             Location/Qualifiers
     source          1..11677
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     33..52
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(271..375)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    412..915
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     primer_bind     957..975
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(1013..1035)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     1135..1154
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     CDS             complement(1648..2742)
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     promoter        complement(2743..3150)
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     rep_origin      complement(3451..3906)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3588..3609)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3800..3819
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4032..4054
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4046..4063
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4047..4063
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4070..4089
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4070..4088
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             10280..10303
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     promoter        complement(11178..11196)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(11217..11233)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11217..11233)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11230..11252)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11241..11257
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11265..11271,11272..11289,11290..11295))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11310..11331
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11448..11465)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
       61 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
      121 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
      181 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
      241 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
      301 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
      361 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gggtcctttt catcacgtgc
      421 tataaaaata attataattt aaatttttta atataaatat ataaattaaa aatagaaagt
      481 aaaaaaagaa attaaagaaa aaatagtttt tgttttccga agatgtaaaa gactctaggg
      541 ggatcgccaa caaatactac cttttatctt gctcttcctg ctctcaggta ttaatgccga
      601 attgtttcat cttgtctgtg tagaagacca cacacgaaaa tcctgtgatt ttacatttta
      661 cttatcgtta atcgaatgta tatctattta atctgctttt cttgtctaat aaatatatat
      721 gtaaagtacg ctttttgttg aaatttttta aacctttgtt tatttttttt tcttcattcc
      781 gtaactcttc taccttcttt atttactttc taaaatccaa atacaaaaca taaaaataaa
      841 taaacacaga gtaaattccc aaattattcc atcattaaaa gatacgaggc gcgtgtaagt
      901 tacaggcaag cgatccgtcc taagaaacca ttattatcat gacattaacc tataaaaata
      961 ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac
     1021 acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag
     1081 cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat
     1141 cagagcagat tgtactgaga gtgcaccata tcgactacgt cgttaaggcc gtttctgaca
     1201 gagtaaaatt cttgagggaa ctttcaccat tatgggaaat ggttcaagaa ggtattgact
     1261 taaactccat caaatggtca ggtcattgag tgttttttat ttgttgtatt tttttttttt
     1321 tagagaaaat cctccaatat ataaattagg aatcatagtt tcatgatttt ctgttacacc
     1381 taactttttg tgtggtgccc tcctccttgt caatattaat gttaaagtgc aattcttttt
     1441 ccttatcacg ttgagccatt agtatcaatt tgcttacctg tattccttta catcctcctt
     1501 tttctccttc ttgataaatg tatgtagatt gcgtatatag tttcgtctac cctatgaaca
     1561 tattccattt tgtaatttcg tgtcgtttct attatgaatt tcatttataa agtttatgta
     1621 caaatatcat aaaaaaagag aatcttttta agcaaggatt ttcttaactt cttcggcgac
     1681 agcatcaccg acttcggtgg tactgttgga accacctaaa tcaccagttc tgatacctgc
     1741 atccaaaacc tttttaactg catcttcaat ggccttacct tcttcaggca agttcaatga
     1801 caatttcaac atcattgcag cagacaagat agtggcgata gggttgacct tattctttgg
     1861 caaatctgga gcagaaccgt ggcatggttc gtacaaacca aatgcggtgt tcttgtctgg
     1921 caaagaggcc aaggacgcag atggcaacaa acccaaggaa cctgggataa cggaggcttc
     1981 atcggagatg atatcaccaa acatgttgct ggtgattata ataccattta ggtgggttgg
     2041 gttcttaact aggatcatgg cggcagaatc aatcaattga tgttgaacct tcaatgtagg
     2101 gaattcgttc ttgatggttt cctccacagt ttttctccat aatcttgaag aggccaaaac
     2161 attagcttta tccaaggacc aaataggcaa tggtggctca tgttgtaggg ccatgaaagc
     2221 ggccattctt gtgattcttt gcacttctgg aacggtgtat tgttcactat cccaagcgac
     2281 accatcacca tcgtcttcct ttctcttacc aaagtaaata cctcccacta attctctgac
     2341 aacaacgaag tcagtacctt tagcaaattg tggcttgatt ggagataagt ctaaaagaga
     2401 gtcggatgca aagttacatg gtcttaagtt ggcgtacaat tgaagttctt tacggatttt
     2461 tagtaaacct tgttcaggtc taacactacc ggtaccccat ttaggaccac ccacagcacc
     2521 taacaaaacg gcatcagcct tcttggaggc ttccagcgcc tcatctggaa gtggaacacc
     2581 tgtagcatcg atagcagcac caccaattaa atgattttcg aaatcgaact tgacattgga
     2641 acgaacatca gaaatagctt taagaacctt aatggcttcg gctgtgattt cttgaccaac
     2701 gtggtcacct ggcaaaacga cgatcttctt aggggcagac attagaatgg tatatccttg
     2761 aaatatatat atatatattg ctgaaatgta aaaggtaaga aaagttagaa agtaagacga
     2821 ttgctaacca cctattggaa aaaacaatag gtccttaaat aatattgtca acttcaagta
     2881 ttgtgatgca agcatttagt catgaacgct tctctattct atatgaaaag ccggttccgg
     2941 cgctctcacc tttccttttt ctcccaattt ttcagttgaa aaaggtatat gcgtcaggcg
     3001 acctctgaaa ttaacaaaaa atttccagtc atcgaatttg attctgtgcg atagcgcccc
     3061 tgtgtgttct cgttatgttg aggaaaaaaa taatggttgc taagagattc gaactcttgc
     3121 atcttacgat acctgagtat tcccacagtt aactgcggtc aagatatttc ttgaatcagg
     3181 cgccttagac cgctcggcca aacaaccaat tacttgttga gaaatagagt ataattatcc
     3241 tataaatata acgtttttga acacacatga acaaggaagt acaggacaat tgattttgaa
     3301 gagaatgtgg attttgatgt aattgttggg attccatttt taataaggca ataatattag
     3361 gtatgtagat atactagaag ttctcctcga ccggtcgata tgcggtgtga aataccgcac
     3421 agatgcgtaa ggagaaaata ccgcatcagg aaattgtaag cgttaatatt ttgttaaaat
     3481 tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa
     3541 tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca
     3601 agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg
     3661 gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta
     3721 aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg
     3781 cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa
     3841 gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg
     3901 gcgcgtccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc
     3961 ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac
     4021 gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgaattgt aatacgactc
     4081 actatagggc gaattggagc tcttgggaag cttcagtacc gcgatttttc agctaatgga
     4141 tcagatgata tataaacaaa aagtgggata cagcatatcg acgcctccac tacaaattaa
     4201 cagaaggatg aaaaccaata agaaaatttc taaaagaaga agcctgaaaa atctccatgg
     4261 tgccttaaaa ggactattga aagagtccgg caagaagagt gagtccaaaa tacgaaaaca
     4321 tagcgattgc aatcctgttc atcgagtata tccgccaaat atcgaaaaac ggaagaccaa
     4381 aaaggacgat ggaatttcac ggccaattgc tgaaagaaat ggtcatgtgt atataatgtc
     4441 caaggaaaac cacatcatcc ctaaactaac cgatgatgaa gtcatggagc gccacaaact
     4501 agcagacgaa aacatgagaa aagtgtggtc caacatcata agcaaatacg agtctattga
     4561 agaacaagga gacctcgtag acctgaaaac tggtgaaata gttgaagata atgggcatat
     4621 caagacgctg acggccaaca acagcacgaa agataaaaga acaaaataca caagcgtgct
     4681 cagagacatt atcgacatca gcgacgagga agatggtgat aaaaatgatg aatatactct
     4741 ttgggctaac gatagcgagg caagcgattc agaggtggat gctgacaacg acactgaaga
     4801 ggaaaaagac gagaaattga tagacgcaga tttcaagaag tacgaggcca aactctcgaa
     4861 aaggatatta cgagattaag gtgctaaaat gccgtatatt cgaatttcat taccatcaga
     4921 atatatatat atatatatga gtctatatta gggactatca tacggtaacc cggacctcca
     4981 ttacccgcat gactttactc actaaagacg attgtcggtt ttaaacattt tttttttttc
     5041 atttcgaccg tcggttctcc ttagtttgtt cagtggcaac aagtggacaa acaagaagag
     5101 cgtgcagcac cattcaccgc cagctttgta tcgtccttta gagtgcagca agaacattaa
     5161 ttgctctcac agaggtatca taagaacatc cgattcgttc attaactgaa atcatatctg
     5221 tgttgttctg atcgactctc tttgttagtt ttatcttcca ttttttttta ttcctttcat
     5281 tttccaataa aatttgaaca ggatcgatat aaacagataa aaaccctcca aagttaacca
     5341 tttgtttata tttgcttttc tgcataagtt ttcctagcga attccctgat caactttcaa
     5401 ggaaaactaa aactactgta ttataagagg tttttttttt cattccagat taattttgaa
     5461 atacgatatc ctcaagttta tctaccagaa tatttgacta agaaatcaaa ctctgttaat
     5521 aataatataa ttataaaaac ctcaactaga aactccaaaa aaaaaaattt accatttttt
     5581 actttctatc cttgttaacc aaatttcaaa aaaattttac cttttctttt tccagaagag
     5641 ggaccaatca taaagatagc aataacactt taccccaaaa tataaatcag acatggtagg
     5701 acaacagtat tctagtgctc cactccgtac agtaaaagag gtccaattcg gtcttttctc
     5761 acctgaagaa gttagagcaa tcagtgtggc caaaattaga tttccagaga caatggatga
     5821 aacccagacg agagcgaaaa ttggtggtct aaacgaccct aggttaggct ctattgatcg
     5881 taatctgaag tgtcaaactt gtcaagaggg tatgaacgaa tgtcctggtc attttggtca
     5941 catagattta gcaaaacctg tatttcatgt tggttttatt gccaaaatta agaaagtatg
     6001 tgagtgtgtc tgtatgcact gtggtaagct attactggat gaacataatg aattaatgag
     6061 acaagctcta gcaatcaaag acagtaaaaa aaggtttgct gcaatttgga ctttatgtaa
     6121 aacaaaaatg gtctgcgaaa cagatgtccc ttctgaagat gatcctactc agctcgtatc
     6181 aaggggaggt tgtggtaata cacagcctac aattcgtaag gatgggttga aattagttgg
     6241 tagttggaaa aaagatagag ccacggggga tgcggatgaa ccagaactaa gagttttaag
     6301 tacggaggaa atcttgaata tttttaagca tatctcagta aaagacttca ctagtttggg
     6361 tttcaacgaa gttttttctc gtccagaatg gatgatttta acatgccttc ctgtcccacc
     6421 accaccggtg cgtccatcca tttccttcaa tgaatctcaa agaggtgagg atgatttaac
     6481 ctttaaactt gctgatattt taaaagctaa tattagtttg gaaacactag agcataacgg
     6541 tgctccacat catgctattg aagaagcaga gagtttatta caatttcatg ttgccactta
     6601 tatggataat gatattgctg gtcaaccaca agctctgcag aagtccggcc gtcccgttaa
     6661 atctattcgt gctcgtttga agggtaaaga gggtcgtatc agaggtaatt taatgggtaa
     6721 gcgtgtggat ttttcggcaa gaactgttat ttctggtgat cctaatttgg aattagacca
     6781 agtcggtgtt ccaaaatcta ttgccaagac tttaacatac ccagaagtgg tcacaccata
     6841 taacatagat cgtctgacgc aacttgttag gaatggacca aatgagcacc ccggtgccaa
     6901 atacgtcatt cgtgatagcg gagaccgtat agatttaaga tacagtaaaa gggcaggtga
     6961 tattcaatta cagtatgggt ggaaagttga acgtcatatt atggacaatg atccagtttt
     7021 attcaaccgt caaccttcgt tgcacaaaat gtccatgatg gcccacagag taaaagttat
     7081 tccatattct acatttagat tgaatttgtc cgttacatct ccatacaatg ccgatttcga
     7141 cggtgacgaa atgaatcttc acgttcctca gtctgaggaa acaagggcgg aactttctca
     7201 attatgtgct gttcctctgc aaattgtttc accacaatct aacaaacctt gtatgggtat
     7261 tgttcaagat actttgtgtg gtattcgtaa actgacatta agagatacat ttatagaact
     7321 tgatcaagtt ttgaatatgc tttattgggt tccagattgg gatggtgtta ttccgacacc
     7381 tgcaattatc aagcccaaac ctttgtggtc cggtaaacaa atcttgtctg tggctatccc
     7441 aaacggtatt catttacaac gttttgatga gggcactact ctgctttctc caaaggataa
     7501 tggtatgctt attattgacg gtcaaatcat ttttggtgta gtagagaaaa aaaccgttgg
     7561 atcctccaat ggtggtttaa ttcatgttgt tacgagagaa aagggacctc aagtttgtgc
     7621 taagttgttt ggtaacatac agaaagttgt taacttttgg ttactacata atgggttttc
     7681 aacaggtatt ggtgatacca ttgcggacgg cccaacaatg agggaaatta cagagacaat
     7741 tgcagaggct aaaaagaaag ttttggatgt tacgaaagaa gcccaggcaa acttattgac
     7801 tgctaaacat ggtatgactc tccgtgagtc ttttgaggat aacgttgttc ggttcctaaa
     7861 tgaagcaaga gataaggcag gtcgtttagc tgaagtcaat ttgaaagatt tgaacaatgt
     7921 gaaacaaatg gttatggcag gttccaaggg ttcatttatt aatatcgcgc aaatgtcagc
     7981 ttgtgtagga cagcaatctg ttgaaggtaa acgtattgct tttgggttcg ttgatcgtac
     8041 cttacctcat ttctctaaag atgattactc cccagagtct aaaggttttg ttgagaactc
     8101 atatttgaga ggtttgaccc cacaagaatt ttttttccat gcaatgggtg gtcgtgaagg
     8161 tcttatcgat accgccgtca aaacagccga aacaggttat attcaacgtc gtttagtgaa
     8221 agctctagaa gatatcatgg ttcattacga taacaccaca agaaactcat tgggtaacgt
     8281 tattcagttt atttatggtg aagatggtat ggatgctgcg catattgaaa agcaatcgct
     8341 agatactatt ggtggctccg atgcagcttt tgaaaagaga tacagagttg atttattgaa
     8401 tacagaccat acccttgatc cctcactatt ggaatccgga tctgagatac ttggcgattt
     8461 gaaacttcaa gttctcctgg atgaagaata caaacaatta gtgaaagatc gtaaattttt
     8521 gagggaagtt tttgttgatg gtgaagcaaa ctggccatta ccagtcaaca taagacgtat
     8581 tattcaaaat gctcaacaaa ctttccacat agatcatacg aaaccatctg atttaacaat
     8641 caaagacatc gttcttggtg taaaggattt gcaagaaaac ttattagtgt tgcgtggtaa
     8701 gaatgaaatt atacaaaatg cccagcgaga tgcagttaca ttgttctgct gtttattacg
     8761 ttcccgtttg gccacacgta gagttctaca agagtacaga ctaacaaaac aggcattcga
     8821 ttgggtatta agtaatatcg aggcacaatt cctccgttct gttgttcacc ctggtgaaat
     8881 ggttggtgtt ctagcagccc aatccattgg tgaaccagcc acacaaatga cccttaacac
     8941 cttccatttt gctggtgttg cttccaaaaa agttacttct ggtgtccccc gtttaaagga
     9001 aattttgaat gtggccaaaa acatgaaaac cccttccttg actgtatact tagagcccgg
     9061 gcatgctgcc gatcaagaac aagcgaagtt gatcagatct gctatcgagc ataccacttt
     9121 aaagagtgtc actattgctt cagaaattta ctatgatcct gatccacgtt ccacagttat
     9181 tccagaagat gaagaaatta tccaacttca tttctcatta ttggatgaag aggctgaaca
     9241 atcttttgac caacaatcac cttggttatt acgtctggaa ctggatcgtg cagcaatgaa
     9301 tgataaagac ttaacaatgg gtcaggttgg tgaaagaatc aagcaaacat tcaaaaatga
     9361 tttgtttgtt atctggtctg aagacaacga tgagaagttg atcatccgtt gtcgtgttgt
     9421 tcgtccaaag tcactagatg ctgagactga agcagaagaa gatcatatgt tgaagaaaat
     9481 tgagaacaca atgttagaga atattacatt acgtggtgta gagaacatcg agcgtgttgt
     9541 catgatgaaa tatgaccgta aagtaccaag tccaactggt gaatacgtta aggaacctga
     9601 atgggtgttg gaaacagatg gtgttaactt atctgaagtt atgactgttc ctggtatcga
     9661 cccaaccaga atctatacca actccttcat tgatataatg gaagttctag gtattgaagc
     9721 tggtcgtgca gccttgtata aagaagttta caatgttatt gcttctgatg gttcgtatgt
     9781 taactaccgt catatggctt tgttagtcga tgttatgaca acccaaggtg gcttaacttc
     9841 tgttactcgt catggtttca acagatcaaa tacaggtgcc ttaatgagat gttcatttga
     9901 agaaactgtc gaaattttgt ttgaagctgg tgcttcagcc gaattagatg attgtcgtgg
     9961 tgtttcggaa aatgtcattc ttggtcaaat ggctccaatc ggtaccggtg catttgatgt
    10021 gatgatcgat gaggagtcac tggtaaaata catgccagaa caaaaaataa ctgagattga
    10081 agacggacaa gatggtggcg tcacaccata cagtaacgaa agtggtttgg tcaatgcaga
    10141 tcttgacgtt aaagatgagc taatgttttc acctctggtt gattcgggtt caaatgacgc
    10201 tatggctgga ggatttacag cgtacggtgg tgctgattat ggtgaagcca cgtctccatt
    10261 tggtgcttat ggtgaagctc tagaagttct gttccagggg ccctttggag tctcctcacc
    10321 aggcttttct ccaacttccc caacatactc tcctacctct ccagcgtact caccaacatc
    10381 accatcgtac tcaccaacat caccatcgta ctcgccaaca tcaccatcgt actcacctac
    10441 atcaccatcg tattcaccaa cgtcaccatc atattcgcca acgtcaccat catattcgcc
    10501 aacgtcgcca tcgtattctc caacgtcacc atcgtattcg ccaacgtcgc cttcctactc
    10561 tcccacgtcg ccaagctaca gccctacgtc tccttcttat tctcctacat ctccatcata
    10621 ctctcctacg tcaccaagtt acagcccaac gtcaccaagt tacagcccaa cgtctccagc
    10681 ctattcccca acatcaccaa gttatagtcc tacatcgcct tcatactctc caacatcacc
    10741 atcctattcc ccaacatcac cttcttactc tcccacctct ccaaactata gccctacttc
    10801 accttcttac tccccaacat ctccaggcta cagcccagga tctcctgcat attctccaaa
    10861 gcaagacgaa caaaagcata atgaaaatga aaattccaga tgatatagta tatcatcctt
    10921 acgtatttga cgttattaca ttatatatag tttctcaaat aatatttcta gtttattttt
    10981 gtatcataat aaaaacgtat accaaatata ccattatttt tcatagcatt atggtaggga
    11041 tagggaatca agtaactaat ttatatccgc agagcattgg gaaaaccaac ggcgctagta
    11101 aatgcattta aattacgtcc gtccaacttc taagcttaag ggtttctcga gggggggccc
    11161 ggtaccagct tttgttccct ttagtgaggg ttaatttcga gcttggcgta atcatggtca
    11221 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
    11281 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
    11341 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
    11401 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
    11461 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
    11521 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
    11581 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
    11641 gacgagcatc acaaaaatcg acgctcaagt cagaggt
//
