LOCUS       sequence-181040-        1114 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Nuclear Huntingtin Chromobody 2: ""iVHH4"" intrabody recognizing 
            amino acids 49–148 of huntingtin, fused to eYFP and nuclear 
            localization signal..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1114)
  AUTHORS   Maiuri T, Mocle AJ, Hung CL, Xia J, van Roon-Mom WM, Truant R
  TITLE     Huntingtin is a scaffolding protein in the ATM oxidative DNA damage 
            response complex.
  JOURNAL   Hum Mol Genet. 2016 Dec 25. pii: ddw395. doi: 10.1093/hmg/ddw395.
  PUBMED    28017939
REFERENCE   2  (bases 1 to 1114)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Hum Mol 
            Genet. 2016 Dec 25. pii: ddw395. doi: 10.1093/hmg/ddw395."
COMMENT     Sequence Label: nucHCB2
FEATURES             Location/Qualifiers
     source          1..1114
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    28..93
                     /label=MCS
                     /note="multiple cloning site"
     CDS             join(481..483,484..486,487..999)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPXXYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIE"
     primer_bind     complement(526..547)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(787..806)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 gctagcgcta ccggtcgcnc catggtgtcc ggactcagat ctcgagctca agcttcgaat
       61 tctgcagtcg acggtaccgc gggcccggga tccatggcag aggtgcagct ggtggagtct
      121 gggggaggct tggtgcagcc tggggggtct ctgagactct cctgtgcagc ctctggattc
      181 actttggatt attatgccat aggctggttc cgccaggccc cagggaagga gcgcgagggg
      241 gtctcatgta ttagtagtag tgatggtagc acatactatg cagactccgt gaagggccga
      301 ttcaccatct ccaganacaa tgccaagaac acggtgtatc tgcaaatgaa cagcctgaaa
      361 cctgaggaca cggccgttta ttactgtgcg acagtccgcg ctccctatag cgactattgt
      421 aatgggtatt atgactactg gggccagggg acccaggtca ccgtctcctc aggaggatcc
      481 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
      541 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
      601 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
      661 ctcgtgacca ccttcggcta cggcctgcag tgcttcgccc gctaccccga ccacatgaag
      721 cagcacgact tcttcaagtc cgccatgccc ganngctacg tccaggagcg caccatcttc
      781 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
      841 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
      901 aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac
      961 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc
     1021 gaccactacc agcananacc cccatcggcg anggccccgt gctgctgccc nanaccncta
     1081 nctganctac cagtccgncc nnancaanna cccc
//
